岛东林场森林碳汇能力分析与评价     

薛杨  王小燕  林之盼  郭海燕  刘宪钊 《热带林业》2012,(2):27-29

为了探讨森林碳汇能力、森林碳汇贮存量,该研究利用IPCC(政府间气候变化专门委员会)制定计算方法,通过对岛东林场2009年森林资源二类清查数据进行分析,得出目前岛东林场碳汇蓄积量约31.4万t。数据分析结果表明,当前岛东林场森林经营方式不能满足国家层面上以应对全球气候变化为目的的多功能森林经营要求,也不能满足海南国际旅游岛建设中对森林游憩资源开发需求,在这基础上提出对岛东林场人工林科学合理的近自然化改造模式。  相似文献   

三达生态型现代农业园农业环境现状调查与评价     

刘长勇  李红敏  玛依拉  代天文  王世俊  薛晓燕 《绿色科技》2012,(7):181-183

以标准NY/T1054-2006为依据,确定了对三达生态型现代农业园的环境调查内容,建立了园区环境监测方案,通过对园区农业环境要素:土壤、种植业灌溉水、畜禽饮用水、农产品加工用水和环境空气的监测评价,结合环境调查结果得出结论:三达生态型现代农业园农业生产环境达到了生产绿色食品的环境要求。  相似文献   

马先蒿无性系建立的研究     

李冬雪  高珂  宋诗迎  徐娜  姜长阳 《绿色科技》2012,(7):53-55

为满足马先蒿观赏栽培对种苗的需要,以生长点为材料,采用组织培养的方法,进行了愈伤组织诱导与分化、试管苗的生根与生根继代增殖培养,以及移栽和定植的研究。结果表明:MS+6-BA0.6mg·L-1+2,4-D1.0mg·L-1+NAA0.3mg·L-1是生长点愈伤组织诱导和分化培养的理想培养基;1/3MS+ABT2号10mg·L-1是不定芽生根培养和试管苗生根继代增殖培养的理想培养基;试管苗移栽和定植的成活率分别为93.4%、98.5%,定植的试管苗保持了马先蒿的植物学性状和观赏性状。  相似文献   

浅谈畜禽病毒病的治疗   总被引：1，自引：0，他引：1  

韩开顺  宋文双  张勃 《中国动物检疫》2012,29(4):70-71

本文从临床兽医角度分析了病毒颗粒结构,病毒的侵入、致病过程,介绍了现在兽医临床上常用的几种抗病毒药物,并根据病毒病的特点,结合笔者临床经验着重介绍了病毒病在不同时期的预防和治疗方案。  相似文献   

Detection and molecular characterization of porcine type 3 orthoreoviruses circulating in South Korea     

Kwon HJ  Kim HH  Kim HJ  Park JG  Son KY  Jung J  Lee WS  Cho KO  Park SJ  Kang MI 《Veterinary microbiology》2012,157(3-4):456-463

Orthoreoviruses infect virtually all mammalian species, causing systemic infections including mild gastrointestinal and respiratory illnesses. However, little is known about the prevalence or genetic diversity of porcine orthoreoviruses in South Korea. We examined 237 diarrheic fecal samples collected from 78 pig farms around the country. RT-PCR utilizing primers specific for the L1 gene of mammalian orthoreoviruses showed that 45 (19.0%) samples were positive. The 10 strains isolated from orthoreovirus-positive samples formed typical perinuclear cytoplasmic inclusion bodies and had an atypical hemagglutination pattern; these are characteristics of type 3 orthoreovirus. Phylogenetic analysis of the S1 gene in these 10 Korean and other strains showed that type 3 orthoreoviruses could be divided into four lineages; the 10 Korean strains were included in porcine lineage IV, along with T3/porcine/Sichuan/2006. Sequence analysis showed that strains in lineage IV had nucleotide identities of 97.0-98.1% and deduced amino acid identities of 96.4-98.2%. Sequence analysis of the σ1 protein, a viral attachment protein, revealed that the amino acid sequences associated with neurotropism (amino acids 198-204, 249I, 350D, and 419E) were highly conserved among the Korean strains, confirming that neural tropism was present. In conclusion, our findings suggest that porcine orthoreovirus infections are endemic in pig farms in South Korea and that the 10 novel Korean porcine orthoreoviruses belong to porcine lineage IV of type 3 orthoreovirus. In addition, sequence analysis of S1 genes encoding the σ1 protein showed that the 9 of 10 Korean porcine orthoreoviruses exhibited neural tropism.  相似文献   

大庆湿地类型及文化旅游价值估算   总被引：1，自引：0，他引：1  

宋立全  张思冲  许瀛元  张人月  邢爽 《森林工程》2012,28(2):83-86

湿地是一种特殊的具有多种功能的生态系统,湿地在保护生物多样性、保持生态环境和可持续发展方面具有重要价值。本文以大庆湿地为研究对象,结合大庆地区2007年LANDSAT TM遥感图形解译数据和实地调查数据,采用地理信息系统技术(GIS),分析大庆湿地分布特征和类型。在此基础上选用费用支出法对大庆湿地文化旅游价值进行了估算:计算结果表明,大庆湿地文化科研价值为4.159 236亿元,大庆湿地旅游价值为7.48亿元,大庆湿地文化旅游价值为11.639 236亿元。  相似文献   

Pharmacokinetic study of luteolin,apigenin, chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract in rats     

Zhongjian Chen  Sisi KongFeifeng Song  Liping LiHuidi Jiang 《Fitoterapia》2012

Flos Chrysanthemi (the flower of Chrysanthemum morifolium Ramat.) is widely used in China as a food and traditional Chinese medicine for many diseases. Luteolin and apigenin are two main bioactive components in Flos Chrysanthemi, and chrysoeriol and diosmetin are two methylated metabolites of luteolin in vivo by cathechol-O-methyltransferase (COMT). However, there was lack of pharmacokinetic information of chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract (FCE). The present study aimed to develop an HPLC-UV method for simultaneous determination of rat plasma concentration of luteolin, apigenin, chrysoeriol and diosmetin and utilize it in pharmacokinetic study of the four compounds after orally giving FCE to rats. The method was successfully validated and applied to the pharmacokinetic study when oral administration of FCE to rats with or without co-giving a COMT inhibitor, entacapone. Chrysoeriol and diosmetin were detected in rat plasma after oral administration of FCE and their concentrations were significantly decreased after co-giving entacapone. Furthermore, AUC of luteolin was significantly increased by entacapone, while that of chrysoeriol was decreased by entacapone, which revealed COMT might play an important role in the disposition of luteolin in rats after dosing of FCE. In conclusion, a sensitive, accurate and reproducible HPLC-UV method for simultaneous determination of luteolin, apigenin, chrysoeriol and diosmetin in rat plasma were developed, pharmacokinetics of chrysoeriol and diosmetin combined with luteolin and apigenin were characterized after oral administration of FCE to rats, which gave us more information on pharmacokinetics and potential pharmacological effects of FCE in vivo.  相似文献   

梅花鹿鹿茸异位生长的试验     

高志光  赵海平  高颂  赵桂炎  杨福合 《中国兽医杂志》2012,48(9):27-29,98

为了揭示鹿茸发生机理,阐明生茸区骨膜在鹿茸发生中的作用,选择4只1周岁雄性梅花鹿,在鹿茸发生前,通过外科手术将一侧完整的生茸区骨膜摘除并移植到额骨上。结果,摘除骨膜的生茸区没有发生鹿茸,而在被移植的额骨部位上发生了鹿茸且生长发育正常。证明了生茸区骨膜是鹿茸发生的组织基础,同时为通过异位生茸提高鹿茸产量开辟了新的途径。  相似文献   

Pharmacokinetic study of baicalein after oral administration in monkeys     

Tian S  He G  Song J  Wang S  Xin W  Zhang D  Du G 《Fitoterapia》2012,83(3):532-540

Baicalein, a flavonoid originally isolated from the root of Scutellaria baicalensis Georgi, has numerous pharmacological activities. Up to now, several studies regarding the pharmacokinetic profiles of baicalein have been described, while there is no such study reported in monkey, the species which is more similar to human. The purpose of this study was to investigate the pharmacokinetic profiles of baicalein after oral administration in monkeys. After orally administrating three doses of baicalein in monkeys, multi-peaks of the plasma concentration-time curves were observed and the non-linear pharmacokinetics for baicalein and its metabolite baicalin were found at doses of 50-500mg/kg. In order to calculate the absolute bioavailability, the intravenous pharmacokinetic study was also carried out after intravenous administration of 10mg/kg baicalein. The absolute bioavailability of baicalein in different doses was ranged from 13.1% to 23.0%. In this study, baicalein and baicalin were determined by LC-MS method. The chromatographic separation was performed on Agilent Poroshell 120 SB-C18 column (2.7μm, 2.1×50mm). Baicalein and baicalin were detected by single quadrupole mass spectrometer equipment with electrospray ionization interface with the selected ion monitoring mode. The assay was linear for both baicalein and baicalin with the correlation coefficients>0.99. The intra- and inter-day precisions for baicalein and baicalin were all less than 15% by relative standard deviation. The analytes were stable during samples storage and handling, and no matrix effects were observed. The method we developed in this study was sensitive, precise, stable and producible.  相似文献   

Lathyrane diterpenes from Euphorbia prolifera and their inhibitory activities on LPS-induced NO production     

J Xu  DQ Jin  H Song  Y Guo  Y He 《Fitoterapia》2012,83(7):1205-1209

A new lathyrane diterpene (1), an unreported spectroscopic data lathyrane diterpenene (2), and two known analaogues (3 and 4) have been isolated from Euphorbia prolifera. Their structures were elucidated as (12E,2S,3S,4R,5R,6S,9S,11S,15R)-3-butyryloxy-5,15-diacetoxy-6,17-epoxylathyra- 12-en-14-one (1), (12E,2S,3S,4R,5R,6S,9S,11S,15R)-3-propionyloxy-5,15-diacetoxy-6,17- epoxylathyra-12-en-14-one (2), (12E,2S,3S,4R,5R,6S,9S,11S,15R)-3-benzoyloxy-5,15-diacetoxy -6,17-epoxylathyra-12-en-14-one (3), and 15-O-acetyl-17-hydroxyjolkinol (4) by spectroscopic methods (IR, ESIMS, HR-ESIMS, NMR, and X-ray crystallography). The inhibitory activities on LPS-induced NO production of these diterpenes were evaluated and compounds 1, 3 and 4 showed inhibitory effects.  相似文献