Incidence of and risks associated with Giardia infections in herds on dairy farms in the New York City Watershed     

Miguella P Mark-Carew  Yasin Khan  Susan E Wade  Stephanie Schaaf  Hussni O Mohammed 《Acta veterinaria Scandinavica》2010,52(1):44

Background

The primary aims of this study were to determine the incidence of Giardia infections in dairy herds on farms in the New York City Watershed region and to evaluate risk factors associated with infections. Because co-infections of Giardia and Cryptosporidium spp. are common in this population, we also evaluated the effect of herd infection status on Giardia infections.

Methods

Farms were grouped into three cohorts based on their prior infection status with Giardia and/or Cryptosporidium spp. The sampling plan included collecting fecal samples from all calves below 30 days of age and proportional sampling of calves, young stock, and adults. A total of 10,672 fecal samples were collected and analyzed for the presence of Giardia cysts using zinc sulfate flotation. Herds enrolled in the study were sampled seasonally for a study period of two years. The probability of shedding cysts past a certain age and the factors that influenced the likelihood of shedding were evaluated using survival analysis. Linear regression was used to evaluate factors that were associated with the intensity of shedding.

Results

The majority of Giardia infections occurred in calves within their first 180 days of age, with the most number of calves shedding Giardia cysts between 11 and 20 days of age. The incidence of shedding of Giardia cysts ranged from 0.0004 per animal day for cattle in the low risk cohort to 0.0011 per animal day for cattle in the high risk cohort. The likelihood of shedding was influenced by the prior infection status of the herd and the season of collection. Infected animals shed on average 9,658 cysts/gram and the intensity of shedding Giardia cysts varied significantly with the age (p < 0.0001) and the season of collection (p = 0.0151 for Spring).

Conclusion

Giardia infections are common in dairy herds in the New York City watershed, particularly in calves less than 6 months of age. Seasonality may be an important factor in the perpetuation of infections based on changes in management practices corresponding to weather patterns of a particular season. A dairy herd''s prior infection status with Cryptosporidium influences the likelihood of infection with Giardia.  相似文献   

Effects of interactions among Metarhizium anisopliae, Bacillus thuringiensis and chlorantraniliprole on the mortality and pupation of six geographically distinct Helicoverpa armigera field populations     

Waqas Wakil  M. Usman Ghazanfar  Tahira Riasat  Mirza Abdul Qayyum  Sohail Ahmed  Muhammad Yasin 《Phytoparasitica》2013,41(2):221-234

A local isolate of Metarhizium anisopliae (Hypocreales: Clavicipitaceae), Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole were assessed against six field populations of tomato fruitworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in a series of laboratory bioassays. Two dose rates of B. thuringiensis (0.5, 1 μg g^?1), one of both M. anisopliae (1.3?×?10⁶ conidia ml^?1) and chlorantraniliprole (0.01 ppm) were applied alone and in combination with each other against 2nd, 3rd, 4th and 5th larval instars. The mortality was observed every 24 h until pupation. The bioassays were carried out at 25°C and 75% r.h. The highest mortality was observed in Rawalpindi with the lowest pupation rate by applying the combined concentrations of B. thuringiensis and chlorantraniliprole. The lowest mortality was observed in population from Gujranwala among all the tested populations. The antagonistic interaction was noted where the high dose rate of B. thuringiensis was combined with M. anisopliae; however, the remaining interactions enhanced the mortality and reduced the percent pupation. The overall results demonstrated that all the treatments gave significant control of the larval instars of H. armigera. The population from Gujranwala proved least susceptible whereas the one from Rawalpindi was highly susceptible.  相似文献   

The importance of reduced light intensity on the growth and development of six weed species     

M Yasin  E Rosenqvist  S M Jensen  C Andreasen 《Weed Research》2019,59(2):130-144

Crops limit light for photosynthesis and growth of weeds. We studied the effect of reduced light on performance of six weed species [one invasive species (Amsinckia micrantha), three common species (Veronica persica, Capsella bursa‐pastoris, Viola arvensis) and two less common weeds (Anagallis arvensis and Scleranthus annuus)]. In two glasshouse experiments, six light levels were achieved aiming at 0%, 20%, 50%, 80%, 90% and 95% reduction of light and corresponding with daily light integrals (DLI) of 12.4, 9.63, 7.13, 2.74, 0.95 and 0.69 mol m^?2 day^?1 in experiment 1 and 21.2, 18.0, 10.7, 3.71, 1.64 and 1.20 mol m^?2 day^?1 in experiment 2. The number of leaves was strictly controlled by DLI. Chlorophyll content index, maximum photochemical efficiency of photosystem II (F_v/F_m), stomatal conductance, flowering and dry matter were strongly reduced when DLI was reduced to 0.69–3.71 mol m^?2 day^?1 for all species. Threshold DLI for flowering was ca. 3.71 mol m^?2 day^?1 for S. annuus, V. arvensis, A. arvensis and V. persica, while C. bursa‐pastoris deviated by flowering at DLI of 0.95 mol m^?2 day^?1. This may explain why C. bursa‐pastoris is common in the seedbank of Danish arable soils in spite of intensive farming with well‐fertilised and dense crops.  相似文献   

Antioxidant and immune potential marker enzymes assessment in the various tissues of rats exposed to indoleacetic acid and kinetin: A drinking water study     

Ismail Celik  Yasin Tuluce  Musa Turker 《Pesticide biochemistry and physiology》2006,86(3):180-185

In the present study, the influence of two different PGRs, indoleacetic acid (IAA) and kinetin (Kn) on immune potential enzymes, adenosine deaminase (ADA) and myeloperoxidase (MPO), and antioxidant defense enzymes such as glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD) in various tissues of rats were investigated during the treatment as a drinking water model. 100 ppm of IAA and Kn as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 21 days continuously. The PGRs treatments caused different effects on the immune potential and antioxidant defense enzymes of experimented rats compared to controls. Results show that IAA caused a significant decrease in GR activity in the lungs and liver and an increase in the spleen. Also, IAA caused a significant decline in GPx activity in the lungs and an increase in the heart. SOD was significantly reduced in the heart, while increased in the lungs. Furthermore, IAA caused a significant decrease in ADA activity in the heart and blood whereas an increase in the kidney and spleen. MPO activity was also significantly increased in the heart by IAA treatment. The activity of enzymes were also seriously affected by Kn; GR activity decreased in the lungs, brain, and blood while GPx activity decreased in the spleen, brain, and heart. ADA activity was also significantly reduced in the blood whereas MPO activity rose in the spleen. In addition, SOD activity lowered in all tissues except for lungs where a significant increment was determined. As a conclusion, the results indicate that PGRs might affect on antioxidant and immune potential enzymes. These data, along with the determined changes suggest that PGRs produced substantial systemic organ toxicity in the erythrocyte, liver, brain, heart, lungs, spleen, and kidney during the period of a 21-day subacute exposure.  相似文献   

The lumbosacral Plexus of the Barn Owl (tyto alba): Formation,branches and distribution     

Yasin Demiraslan  İftar Gürbüz  İsmail Demircioğlu  Mustafa Orhun Dayan 《Anatomia, histologia, embryologia》2021,50(3):572-578

The aim of the present study is to reveal the anatomical structure of lumbosacral plexus of barn owl. Six barn owls were included in the study. Nerves originating from plexus were dissected individually, and findings of innervation areas of nerves were determined. Lumbosacral plexus of barn owl was observed to be formed by ventral rami of ten synsacral spinal nerves. It was found that while the r. cutaneus femoris lateralis, the n. obturatorius, the n. coxalis cranialis, the r. cutaneus femoris medialis, the n. cutaneus femoris cranialis and the n. femoralis originated from the lumbar plexus, the n. isciofemoralis, the n. cutaneus femoris caudalis, the n. coxalis caudalis and the n. ischiadicus originated from the sacral plexus. Consequently, when the results of the study were compared with information of different avian species, it was observed that differences focused on the formation of the lumbar and sacral plexuses and innervation level of digits.  相似文献   

Resistance to common insecticides and mechanisms of resistance in Aphis pomi de Geer (Hemiptera: Aphididae), in apple orchards in Turkey     

Erdogan  Cem  Ozdem  Ayse  Alpkent  Yasin Nazim  Demiroz  Duygu 《Phytoparasitica》2023,51(2):323-335

Phytoparasitica - The Green apple aphid, Aphis pomi de Geer (Hemiptera: Aphididae), is one of the most common and serious insect pests of apple orchards in Turkey. This species feeds on succulent...  相似文献   

Daylight crop water stress index for continuous monitoring of water status in apple trees     

Yasin Osroosh  Robert T. Peters  Colin S. Campbell 《Irrigation Science》2016,34(3):209-219

We studied the suitability of empirical crop water stress index (CWSI) averaged over daylight hours (CWSI_d) for continuous monitoring of water status in apple trees. The relationships between a midday CWSI (CWSI_m) and the CWSI_d and stem water potential (ψ _stem), and soil water deficit (SWD) were investigated. The treatments were: (1) non-stressed where the soil water was close to field capacity and (2) mildly stressed where SWD fluctuated between 0 and a maximum allowable depletion (MAD of 50 %). The linear relationship between canopy and air temperature difference (ΔT) and air vapor pressure deficit (VPD) averaged over daylight hours resulted in a non-water-stressed baseline (NWSBL) with higher correlation (?T = ?0.97 VPD – 0.46, R ² = 0.78, p < 0.001) compared with the conventional midday approach (?T = ?0.59 VPD – 0.67, R ² = 0.51, p < 0.001). Wind speed and solar radiation showed no significant effect on the daylight NWSBL. There was no statistically meaningful relationship between midday ψ _stem and CWSI_m. The CWSI_d agreed well with SWD (R ² = 0.70, p < 0.001), while the correlation between SWD and CWSI_m was substantially weaker (R ² = 0.38, p = 0.033). The CWSI_d exhibited high sensitivity to mild variations in the soil water content, suggesting it as a promising indicator of water availability in the root zone. The CWSI_d is stable under transitional weather conditions as it reflects the daily activity of an apple crop.  相似文献   

Effect of Different Nitrogen Doses on Dry Matter Ratio,Chlorophyll and Macro/Micro Nutrient Content in Sweet Herb (Stevia rebaudiana Bertoni)     

Esra Ucar  Yasar Ozyigit  Ahmet Demirbas  Dilek Yasin Guven  Kenan Turgut 《Communications in Soil Science and Plant Analysis》2017,48(10):1231-1239

With this study, the effect of different nitrogen (N) doses applied to sweet herb (0, 50, 100, 150 and 200 N kg ha^?1) on the macro/micro nutrient element content, dry matter ratio and on the chlorophyll amount in the plant leaves is examined. The research was carried out in the research and trial field of Faculty of Agriculture Agronomy department, Akdeniz University, during the 2012–2013 growing season under field conditions for 2 years with four replications according to the randomized block trial design. Chlorophyll amounts were determined and the average was obtained with three readings from each leaf by a chlorophyll meter when the plants were in the beginning of flowering. Post-harvest macro and micro nutrient elements of the sweet herb were also investigated. According to the obtained data, while the highest chlorophyll and N amounts in the leaf for both the first and second years were determined as 46.97 SPAD (Minolta Corp, NJ, USA) and 1.34 N% 44.9 SPAD, 1.42 N%, respectively, under 200 kg ha^?1 N application, the lowest chlorophyll and N amounts were determined in the control group. In addition, N application in different doses for both years increased the phosphorus (P) and potassium (K) contents and increased the microelement contents, especially in the second year. At the same time, a correlation between the chlorophyll content in the plant leaves and the N amount was determined and a linear increase was observed in the chlorophyll amount with the increased green part.  相似文献   

近16年来新疆耕地与人口变化相关分析   总被引：1，自引：1，他引：0  

吐尼沙古丽·牙生  满苏尔·沙比提 《干旱区资源与环境》2010,(5)

利用1990～2006年的统计数据,采用统计分析方法,建立了耕地与人口相关模型,在此基础上分析了近16年来新疆的耕地变化过程、地域分异特征、耕地与人口相关性变化趋势、以及不同地区耕地与乡村人口相关的区域差异。结果表明:近16年来新疆耕地面积呈现波动增加趋势,但增加速度缓慢,不同时段耕地年均递增率呈现高-低-高-低模式,耕地变化地域差异特征明显;利用2006年耕地与对应的乡村人口数据进行相关分析,发现耕地面积与乡村人口数之间存在着极显著的正相关关系;1990～2006年新疆耕地面积与乡村人口之间的相关性呈正相关,并达到了0.01水平的显著性,且不同地区乡村人口数与耕地面积之间的相关系数在不同时期差别较大;1990～2006年期间,人均耕地面积减少了0.0874hm2,年均递减率为1.46%,而劳耕密度呈现增长趋势,净增加0.1926人/hm2,年递增率为0.2754%,在不同时段人均耕地面积及劳耕密度的变化幅度及趋势不同。  相似文献   

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling,Lates calcarifer     

Yong Kit Chin  Nurhidayu Al‐saari  Zarirah Zulperi  Aslizah Mohd‐Aris  Annas Salleh  Santha Silvaraj  Aslah Mohamad  JingYie Lee  Mohd Zamri-Saad  Md Yasin Ina-Salwany 《Aquaculture Research》2020,51(1):389-399

Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh.  相似文献