Generalized Alopecia with Vasculitis-Like Changes in a Dog with Babesiosis     

Yumi TASAKI  Naoki MIURA  Keita IYORI  Koji NISHIFUJI  Yasuyuki ENDO  Yasuyuki MOMOI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(10):1367-1369

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利用农杆菌介导Bar基因转化稻瘟病菌*     

袁  远；辻元人；久保康之；何月秋 《云南农业大学学报(自然科学版)》2007,22(5):760-761

 用携带除草剂抗性基因（Bar基因）的质粒pBIG4MRBrev和农杆菌EHA105，建立了农杆菌介导Bar基因转化稻瘟病菌的转化体系，使稻瘟病菌菌株95－8－36得到高效转化，且能稳定表达。  相似文献   

Pyridinoline concentrations in muscular and skin collagen of fish and relationship between collagen solubility and pyridinoline concentration in fish muscular collagen     

Masashi Ando  Yoko Nakagishi  Keiko Yoshida  Masashi Nakao  Takayuki Nakagawa  Yasuo Makinodan  Yasuyuki Tsukamasa  Ken-Ichi Kawasaki 《Fisheries Science》2006,72(5):1104-1108

Pyridinoline (Pyr), one of the mature crosslinks of collagen, was determined in muscular collagen of three species of fish. The amounts of muscular Pyr in red sea bream, yellowtail, and tiger puffer were 3.4, 8.8, and 50.3 mmol/mol collagen, respectively, indicating that the Pyr concentration in muscular collagen differs greatly among fish species. The Pyr concentration in tiger puffer muscular collagen was the greatest, but it was only one-fourth that in rabbit muscle. As in mammalian skin collagen, Pyr was not detected in skin collagen of red sea bream and yellowtail. However, tiger puffer skin contained Pyr (3.75 mmol/mol collagen). The presence of Pyr would have a relationship to some features of tiger puffer skin, such as mechanical strength and thickness. Pyr concentrations in acid-soluble collagen (ASC), pepsin-solubilized collagen (PSC), and insoluble collagen (ISC) in muscles of three species of fish were determined. Pyr was found in ISC > PSC > ASC, from the highest to the lowest concentration, and the concentration in ISC was 45–200 times that in ASC. Therefore, Pyr crosslinks that are formed between collagen molecules would have a close relationship to collagen solubility.  相似文献   

Effects of microbial growth inhibition by antibiotics on the production of taste-active components during the processing of heshiko produced by aging salted mackerel with rice bran     

Kosaka  Yasuyuki  Ooizumi  Tooru 《Fisheries Science》2012,78(3):735-742

To elucidate the role of microbes and endogenous proteases in the formation of the taste-active components during processing of heshiko, a Japanese traditional seafood produced by aging salted mackerel with rice bran, the production of lactic acid, free amino acids, and acid-soluble peptides throughout the aging process was compared in the absence and presence of antibiotics including chloramphenicol, penicillin, and cycloheximide. Although viable counts on fish flesh on a 2.5% NaCl-GYP agar plate increased to 10⁶ cfu/g with the aging process of heshiko in the absence of the antibiotics, adding the antibiotics suppressed the increase in viable counts to less than 10³ cfu/g throughout the aging process. Lactic acid barely increased due to the suppression of microbial growth by the antibiotics. In spite of the inhibition of bacterial growth throughout the processing of heshiko upon the addition of the antibiotics, little difference was observed in the production of free amino acids and acid-soluble peptides. These results suggest that, unlike lactic acid (which is a fermentation product of lactic acid bacteria), free amino acids and acid-soluble peptides are mainly produced through protein hydrolysis by endogenous proteases in mackerel meats during the aging process of heshiko.  相似文献   

Paragonimus ohirai metacercariae in crabs collected along the Arakawa River in Tokyo, Japan     

Sugiyama H  Morishima Y  Kameoka Y  Arakawa K  Kawanaka M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):927-931

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Effects of thiosulfate addition on ammonia and nitrogen removal in biofilters packed with Oyaishi (pumice tuff)     

Tomoko Yasuda  Yasuyuki Fukumoto  Miyoko Waki  Toshimi Matsumoto  Hirohide Uenishi 《Animal Science Journal》2020,91(1)

Ammonia removal is achieved partly by absorption and nitrification in biofilters, resulting in the accumulation of nitrogen and the necessity of treating the effluent water. We investigated the effects of thiosulfate addition to a biofilter containing pumice tuff for ammonia and nitrogen removal in a laboratory‐scale experiment. The addition of thiosulfate to the circulating water led to a decreased nitrate and nitrite along with an increase of sulfate. The inorganic nitrogen in the circulating water decreased by up to 44% with thiosulfate addition compared to without thiosulfate. Batch experiments revealed that denitrification activity decreased exponentially along with increases in dissolved oxygen; however, approximately 30% of denitrification activity was maintained at dissolved oxygen concentration of 3.3 mg/L. Metabarcoding of 16S rRNA genes indicated that the genus Thiobacillus had a relative abundance of 0.002%–0.016% of total bacteria in the biofilter packing material. The circulating water pH was decreased below 5 with sulfur oxidation, and ammonium was accumulated without pH control resulting in a decrease in the relative abundance of the family Nitrosomonadaceae. Its relative abundance increased with control of pH to near neutral, indicating that ammonia‐oxidizing activity could be maintained by adjusting pH. Thiosulfate addition could stimulate nitrogen removal by sulfur‐dependent denitrification in biofiltration systems.  相似文献   

Rapid visualization of mammary gland tumor lesions of dogs using the enzyme-activated fluorogenic probe; γ-glutamyl hydroxymethyl rhodamine green     

Yui HIROSE  Mona UCHIDA  Masaya TSUBOI  Takayuki NAKAGAWA  Leo YAGA  Shingo MAEDA  Yasuyuki MOMOI  Yugo KURIKI  Mako KAMIYA  Yasuteru URANO  Tomohiro YONEZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):593

Since gamma-glutamyl transpeptidase (GGT) is highly and locally expressed in human breast cancer, a GGT-enzymatically activatable fluorescent probe, gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), has been developed to detect the human breast cancer area with high performance. In this study, GGT expression and the efficacy of gGlu-HMRG on visualization were investigated in canine mammary gland tumors (MGT). Seventeen non-fixed fresh-frozen MGT specimens and each peritumoral control tissue were utilized. The GGT mRNA levels were highly observed in the tumor specimens compared with the control. GGT immunostaining was mostly observed on the cell membrane and cytosol of the alveolar and duct mammary epithelium of MGT tissues. These signals were strongly positive in several cases while they were mild to not observed in other cases. When gGlu-HMRG solution was dropped to the non-fixed tissue pieces of MGT or control tissues, the fluorescence intensities (FIs) were measured using Maestro in-vivo imaging device. FIs in MGT tissues were significantly higher than each control tissue 20 min after treatment. Based on Youden index method said that the maximum sensitivity and specificity of FI was 82.4% and 82.4%. These findings suggest that GGT is highly expressed in several MGTs in dogs and gGlu-HMRG could visualize at least a part of MGT tissues in dogs. Nevertheless, it should be needed to assess the false-negative areas more carefully in canine than human cases.  相似文献   

Interspecific variation in wildlife responses to cattle,swine and chicken feed in the forests surrounding poultry farms     

Shinsuke H. SAKAMOTO  Yasuyuki MIYAMOTO  Rina UKYO  Seiji IEIRI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):653

To strengthen farm biosecurity, wildlife behaviors around livestock environments require significant attention. Livestock feed is considered one of essential factors that attract wildlife to the livestock environment. We experimentally studied wildlife response to cattle, swine, and chicken concentrated feeds in the forests surrounding poultry farms. In 14 feeding sites, four feed conditions were established: without feed (control); cattle feed; chicken feed; and swine feed. Wildlife behaviors at each feed point were monitored using infrared cameras. In 3,175 videos, 10 mammals were photographed on 10 or more occasions. Wildlife more frequently appeared at the points with feed than without feed. In addition, the number of videos that captured foraging or interest behaviors was largest for swine feed, followed by chicken feed, then cattle feed. There was a large difference among wildlife in their response to livestock feeds, although each species did not have a strong preference for a specific feed. Livestock feeds invite frequent visits by high and moderate response groups, especially omnivores and carnivores with omnivorous tendencies. Therefore, to protect against such wildlife intrusion, leftover feed and feed storage must be properly managed. This study also suggests that livestock feeds may not cause intrusions by rare response group species; hence, if their intrusions occur, they may be due to factors other than livestock feed. The study situation can partly reflect actual feed-stealing situations. The results will contribute to consider the properly management to protect livestock environments from wildlife intrusions.  相似文献   

Sorafenib inhibits tumor cell growth and angiogenesis in canine transitional cell carcinoma     

Shohei YOKOTA  Tomohiro YONEZAWA  Yasuyuki MOMOI  Shingo MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):666

Canine transitional cell carcinoma (cTCC) is the most common naturally occurring bladder cancer and accounts for 1–2% of canine tumors. The prognosis is poor due to the high rate of invasiveness and metastasis at diagnosis. Sorafenib is a multi-kinase inhibitor that targets rapidly accelerated fibrosarcoma (RAF), vascular endothelial growth factor receptor (VEGFR)-1, VEGFR-2, VEGFR-3, platelet-derived growth factor receptor-β (PDGFR-β), and KIT. In previous studies, a somatic mutation of B-rapidly accelerated fibrosarcoma (BRAF) and expressions of VEGFR-2 and PDGFR-β were observed in over 80% of patients with cTCC. Therefore, in this study, we investigated the anti-tumor effects of sorafenib on cTCC. Five cTCC cell lines were used in the in vitro experiments. All five cTCC cell lines expressed VEGFR-2 and PDGFR-β and sorafenib showed growth inhibitory effect on cTCC cell lines. Cell cycle arrest at the G0/G1 phase and subsequent apoptosis were observed following sorafenib treatment. In the in vivo experiments, cTCC (Sora) cells were subcutaneously injected into nude mice. Mice were orally administered with sorafenib (30 mg/kg daily) for 14 days. Sorafenib inhibited tumor growth compared to vehicle control. The necrotic area in the tumor tissues was increased in the sorafenib-treated group. Sorafenib also inhibited angiogenesis in the tumor microenvironment. Thus, sorafenib may be potential therapeutic agent for cTCC via its direct anti-tumor effect and inhibition of angiogenesis.  相似文献