High efficient expression of human endostatin in E.coli and its antiangiogensis activity     

ZHANG Ge  KE Xi-song  DING Ai-ling  YING Wei  YANG kun  ZHU Zhen-yu 《园艺学报》2002,18(11):1373-1375

AIM: To examine the expression of human endostatin in E.coli, produce its fusion protein antibody and observe its biological activity. METHODS: Endostatin gene was amplified by polymerase chain reaction,recombined with plasmid vector pGEX-2T and induced expression with IPTG.The protein activity was tested by endothelial cell proliferation inhibitory assay.Inclusion body crudely purified was used to generate polyclonal antibody to detect its expression at mouse's liver and kidney etc. RESULTS: The protein expressed was 20kD after digestion by thrombin,it appeared the anti-angiogenesis activity and Western blotting indicated the expression of endostatin in liver and kidney of mouse. CONCLUSION: The successful expression of human endostatin and the preparation of polycolonal antibody indicated its potential application in anti-angiogenesis therapy and diagnosis tumors.  相似文献   

Study on the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes of mouse     

WANG Xiang  ZENG Qiu-tang  ZHU Wu-qiang  CAO Lin-sheng 《园艺学报》2002,18(9):1085-1089

AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D₃ line(ES-D₃) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D₃ cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D₃ cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC.  相似文献   

Changes of myocardial glycogen content and right ventricular function in hypoxia swimming rats     

ZHOU Xiao-mei  MA Ning  ZHU Long-kun  QI Bao-shen 《园艺学报》2002,18(1):21-23

AIM: To investigate the changes of myocardium glycogen content and the relation ship between changes of the myocardial glycogen content and the myocardial function. METHODS: Male Wistar rats were placed in (hypoxia rats) and made to swim in (hypoxia swimming rats) a hypobaxic chamber simulating an altitude of 5 000 m above sea level. The content of myocardium glycogens was determined by colorimetry. RESULTS: The myocardium
glycogen content of rats significantly reduced along with the prolongation of hypoxic exposure and approached to control level in hypoxia swimming rats. The myocardial function of right ventricule was improved significantly compared with control group.CONCLUSION: Moderate exercise (swimming) is beneficial to hypoxic adaptation of rats under the condition of chronic hypoxia.  相似文献   

Effects of angiotensin II-induced hypertension chemotherapy on the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas     

SHANG Zhan-ping  HOU Zhong-chi  ZHU Shi-gong  JI Guo-yi  WANG Jian-chun  ZHAO Wan 《园艺学报》2002,18(12):1521-1523

AIM: To investigate the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas under angiotensin II-induced hypertension chemotherapy. METHODS: The brain tumor model was produced in Wistar rats by stereotaxic inoculation of C6 glioma cells (1×10¹² /L). Tumor-bearing rats were treated with carmustine, teniposide and lisplatin (chemotherapy) during angiotensin II-induced hypertension. Then, the survival time of tumor-bearing rats, tumor blood flow, the concentration of drug, volume of gliomas and the activity of interleukin-1β converting enzyme in glioma were examined.RESULTS: The survival time of tumor-bearing rats was significantly longer in chemotherapy with angiotensin II-induced hypertension group than that of chemotherapy alone. In addition, regional tumor blood flow, the concentration of chemotherapeutic drug and the activity of interleukin -1β converting enzyme in transplanted rat gliomas were increased, while the volume of gliomas was decreased in hypertention chemotherapy group compared with chemotherapy alone. CONCLUSION: Chemotherapy with angiotensin II-induced hypertension has a enhancing effect on chemotherapy for improving the drug delivery to tumor tissue by a increased tumor blood flow and enhancing activity of interleukin -1β converting enzyme.  相似文献   

Effects of hydrogen peroxide on cell proliferation and expression of gelatinase A and its inhibitor in vascular smooth muscle cells     

GUO Xiao-gang  CHEN Jun-zhu  ZHU Jian-hua  ZHENG Liang-rong  ZHANG Fu-rong  TAO Qian-min  ZHANG Yun 《园艺学报》2002,18(12):1524-1528

  相似文献   

黄花蒿粗提物对几种害虫拒食性的初步研究   总被引：10，自引：0，他引：10  

朱芬  雷朝亮等 《昆虫天敌》2003,25(1):16-19

以黄花镐为原料进行浸提，经生物活性测定，结果表明黄花蒿Artenisia annua L.粗提物对供试的6种害虫均具有拒食性。其中对黑翅土白蚁Odontotermes formosanus Shiraki、赤拟谷盗Thibolium castaneum Herbst、谷蠹Rhizopertha dominica Fabricius拒食性极强，对棉蚜Aphis gossypii Glover、棉红蜘蛛Tetranychus urticae Koch及豇豆荚螟Etiella zinckenella Treitschke 也具有较强的巨食性。使用黄花蒿粗提物时，以稀释500倍和800倍效果最好，处理与对照之间有极显著差异。  相似文献   

生物传感器在农药残留分析中的应用   总被引：13，自引：0，他引：13  

李颖娇  张荣全  叶非 《农药科学与管理》2003,24(8):11-13

本文简要介绍了几类生物传感器（酶传感器、微生物传感器、免疫传感器及压电传感器）在农药残留分析中的应用进展，并探讨了其发展趋势。  相似文献   

中生菌素对水稻白叶枯病的防治机制   总被引：6，自引：1，他引：6  

蒋细良  朱昌雄  姬军红  孙东园  魏刚  田云龙 《中国生物防治》2003,19(2):69-72

对不同白叶枯病抗性水稻品种用200μg/ml中生菌素55℃温汤药液浸种，自然降温，秧田3—4叶期和移栽前5d各用30μg/ml中生菌素处理后，于成株期剪叶接种白叶枯病菌。结果表明中生菌素前期处理，于成株期接种白叶枯病菌时，高抗、中等抗性和感病品种中过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶3种酶活性都有不同程度的提高，且以中等抗性品种酶活提高最多，接种24和48h，3种酶活性较清水对照分别增加26．92％、26．74％、24．06％和7．09％、1．31％、1．60％。盆栽试验表明，中生菌素对中抗品种的防治效果最好，达58．4％。说明中生菌素对水稻防御酶活性的诱激作用是其防治白叶枯病的机制之一。  相似文献   

节能日光温室中番茄灰霉病发生规律的研究   总被引：9，自引：2，他引：9       下载免费PDF全文

李宝聚  朱国仁  关天舒  赵奎华 《植物保护》2003,29(2):26-29

通过 3年对节能日光温室环境温湿度监测、病菌孢子捕捉和番茄灰霉病发生规律研究明确 ,一般年份 ,灰霉病在番茄叶片上表现为明显的始发期、盛发期和末发期等 3个阶段 ;果实发病后 ,即进入盛发期。温室中病菌的发生与病害呈正相关 ,病菌的高峰期较病害的高峰期提前10～15d。持续的低温、高湿、苗期带病等是引起番茄灰霉病发生的重要因子。提出要从优化设施环境等方面来控制番茄灰霉病的危害。  相似文献   

哈茨木霉对水稻恶苗病菌的拮抗作用   总被引：11，自引：0，他引：11       下载免费PDF全文

产祝龙  丁克坚  檀根甲  苏贤岩  朱圣杰  陈琪 《植物保护》2003,29(3):35-39

PDA平板拮抗试验表明 ,哈茨木霉对水稻恶苗病菌有强烈的拮抗作用 ,其孢子悬浮液的含孢量为 10⁶～10⁷个 /mL时 ,对恶苗病菌的抑制力达 92.33%。通过哈茨木霉菌液和 3种药剂对水稻恶苗病菌抑制效果的比较 ,哈茨木霉孢子悬浮液含孢量为 10⁷个 /mL与施保克质量浓度 1μg/mL的抑菌效果接近 ,分别为 76.7%、75.4%。显微摄影结果显示 ,哈茨木霉以附着胞附着在恶苗病菌菌丝上 ,然后穿透菌丝在其内生长 ,或与恶苗病菌的菌丝平行生长 ,然后再侵入病菌内寄生。  相似文献