新型绿色饲料资源——海带     

赵京辉  高振华 《饲料工业》2007,28(14):59-61

<正>海带是褐藻门、海带目、海带科、海带属的一种大型海藻,生长在海底的岩石上,形状像带子,因此得名海带,别名纶布,药用称昆布,为一种大型食用藻类。海带在我国有着比较悠久的食用历史,具有丰富的营  相似文献   

鸭病毒性肝炎的发生与防治   总被引：2，自引：0，他引：2  

赵守山  孙红霞  陈守亮 《水禽世界》2007,(8):27-27

鸭病毒性肝炎是由鸭肝炎病毒引起雏鸭的一种传播迅速和高度致死性传染病。本病主要感染3周龄以内的雏鸭,3～10日龄雏为高感群体,潜伏期为1～4d。雏鸭突然发病,神经症状明显,运动失调,身体倾向一侧,双腿做痉挛性运动,呈角弓反张姿势,俗称"背脖病"。该病的发病率和死亡率都较高,一旦鸭群发病后,能迅速传播,引起大批死亡。  相似文献   

快速鉴别诊断山羊痘病毒和羊口疮病毒二联PCR方法的建立   总被引：2，自引：1，他引：2  

朱伟  熊毅  刘棋  郭建刚  李华明 《中国预防兽医学报》2007,29(5):394-396

参照GenBmk已发表的山羊痘病毒(GPV)和羊口疮病毒(ORF)的核苷酸序列设计了两对引物,建立了一种可以快速鉴别山羊痘病毒和羊口疮病毒二重PCR方法,检测结果显示,对山羊痘病毒和羊口疮病毒可以分别扩增出413 bp和595 bp的特异性片段,经敏感性测定,最低能检测到4 pg的DNA。对广西的送检的山羊病料20份进行检测,其中7份可扩增出413 bp的山羊痘特异片段、1份可扩增出595 bp的羊口疮特异片段。该方法能在4 h内完成整个检测过程,不仅快速,而且特异强、敏感性高,很适合于快速诊断。  相似文献   

叶面喷施硼酸对苹果果实硼和钙含量的影响   总被引：2，自引：0，他引：2  

魏宗梅  许雪峰  李天忠  王忆  孔瑾  韩振海 《园艺学报》2007,34(5):1111-1116

 以‘富士’苹果为试材，通过幼果期、果实膨大期和果实成熟期叶面喷施硼酸，研究硼对果实硼和钙含量的影响。结果表明：从叶面喷硼对果实吸收硼的短期效果来看，果实发育的不同时期叶面喷施硼酸均促进果实对硼、钙的吸收，并且处理的效果顺序是：幼果期>果实膨大期>果实成熟期；在果实采收时硼含量为：果实膨大期>果实成熟期>幼果期。不同时期叶面喷施硼酸均提高了果肉细胞中水溶态硼、半束缚态硼和束缚态硼的绝对含量，从3种形态硼所占的比例来看，随着果实不断发育，水溶态硼和半束缚态硼比例升高，而束缚态硼比例下降。  相似文献   

小白菜新品种京绿7号的选育     

赵岫云  徐家炳  张凤兰  余阳俊  张德双  卢桂香 《中国蔬菜》2007,1(11):33-34

京绿7号是由来源于上海和北京的两个地方品种选择的自交不亲和系Y134和Y105杂交而成。植株半直立,株高24cm,开展度37cm;叶深绿色、有光泽,叶面稍皱,叶柄绿色、有光泽、宽5.7cm、厚1.0cm;单株质量0.25kg左右,每667m2产量2400kg左右;高抗黑腐病,抗病毒病、黑斑病,晚抽薹性极强,适宜北方地区冬春季栽培。  相似文献   

Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers     

Li-Wang Liu  Yan Wang  Yi-Qin Gong  Tong-Min Zhao  Guang Liu  Xiao-Yan Li  Fan-Min Yu 《Scientia Horticulturae》2007

Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed genetic purity of two commercial hybrid tomato (Lycopersicon esculentum L.) cultivars ‘Hezuo 903’ and ‘Sufen No. 8’. Genomic DNA from the two F₁ hybrid cultivars and their corresponding parental lines was screened with 218 RAPD decamer primers, 54 ISSR primers and 49 SSR primers. Among the 321 primers, 4 primers for ‘Hezuo 903’ and 3 for ‘Sufen No. 8’, which could produce both female and male parent-specific markers, were selected for testing the genetic purity. A total of 210 hybrid individuals of each cultivar were analyzed using the identified primers. The combined results of the marker analysis showed that eight of the 210 F₁ plants in ‘Hezuo 903’ and 13 of 210 in ‘Sufen No. 8’ were false hybrids, and the overall genetic purity of the two F₁ hybrid seed lots was 96.2 and 93.8%, respectively. This study showed that RAPD and SSR markers could provide a practical and efficient tool in quality control of the tomato commercial hybrid seeds.  相似文献   

VEGF induces HUVECs to produce extracellular H₂O₂ and its proliferation role     

QIAN Zhong-qing  ZENG Yao-ying  WANG Tong  LIN Yi  JI Yu-hua  ZHAO Jing-xian 《园艺学报》2007,23(3):533-535

AIM:To study the effect of VEGF on extracellular H₂O₂ production in HUVECs and the role of H₂O₂ in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H₂O₂ was detected by H₂DCFDA staining. MTT method was used to value the influences of 3×10⁶ U/L catalase and 5-20 mmol/L H₂O₂ to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×10⁶ U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×10⁶ U/L catalase. The extrinsic H₂O₂ at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H₂O₂ and plays role in proliferation, but extrinsic H₂O₂ restrains VEGF function.  相似文献   

Protective effect of atrial natriuretic peptide on alveolar type II cells     

YAN Zhi-qiang  WEI Min  LI Zhi-chao  LI Zhi-bin  LIU Yi  ZHANG Bo  ZHANG Qi  PENG LI-jing  LUO Ying 《园艺学报》2007,23(6):1111-1115

AIM: To study the protective effect of atrial natriuretic peptide (ANP) on alveolar type II cells (AT-Ⅱ) damaged by lipopolysaccharide (LPS).METHODS: AT-Ⅱ were placed in a 6 well cell culture cluster (0.5×106 cells/cm2) and divided into 3 groups: (1) control group (n=6), the medium consisted of RPMI-1640 without FBS. (2) LPS group (n=6), the medium consisted of RPMI-1640 without FBS supplemented with LPS (1 mg/L). (3) ANP group (n=6), the medium consisted of RPMI-1640 without FBS supplemented with LPS (1 mg/L) and ANP (10-8, 10-7, 10-6 mol/L). After 4, 12 and 24 h, the cell culture mediums of control group, LPS group and ANP (10-7 mol/L) group were collected, and those of the ANP (10-6, 10-8 mol/L) group were collected after 12 h. Alkaline phosphatase(AKP), lactate dehydrogenase(LDH), malondialdehyde(MDA), total phospholipids (TPL) and surface tension (ST) in the medium of every group were examined. RESULTS: AT-Ⅱ were characterized by AKP staining. The contents of LDH, AKP and MDA in the medium of every ANP group were lower than those in the corresponding LPS group. The TPL content in the medium of every ANP group was higher than that in the corresponding LPS group, and the change of ST of the medium was opposite to that of TPL. The effect at 12 h was the most significant, for example, at 12 h, the activities of AKP in the mediums were: control (43.5±10.4) U/L, LPS (98.1±16.4) U/L, LPS+ANP (10-6) (46.4±10.5) U/L, LPS+ANP(10-7) (60.7±9.5) U/L, LPS+ANP(10-8) (91.3±13.9) U/L.CONCLUSION: ANP protects the AT-Ⅱ from being damaged by LPS and promotes the secretion of pulmonary surfactants.  相似文献   

Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes     

XIONG Long-gen  ZHANG Yi  SU Tao  LIU Shi-ming  DONG Qi  CHEN Min-sheng 《园艺学报》2007,23(7):1254-1257

AIM: To investigate the effect of neuropeptide Y (NPY) on intracellular free calcium(［Ca²⁺］_i) and Ca²⁺ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS: Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h. Fluorescent indicator Fluo-4 AM was used to detect ［Ca²⁺］_i and Fluo-5N AM was used to detect Ca²⁺ in sarcoplasmic reticulum (SR). Calcium image was recorded by laser scanning confocal microscope. The SR Ca²⁺ load was estimated by caffeine-induced Ca2+ transient (CCT). RESULTS: 24 h after incubation with NPY, compared with control group, the concentration of ［Ca²⁺］_i was significantly elevated (P<0.05), and the concentration of free Ca2+ in SR (［Ca²⁺］_SR) was significantly decreased (P<0.05), and the peak of CCT was attenuated.CONCLUSION: Stimulation with NPY for 24 h causes redistribution of free calcium in rat cardiomyocytes, namely the elevation in ［Ca²⁺］_i and decline in ［Ca²⁺］_SR.  相似文献   

uclear factor κB in LPS stimulated rat alveolar macrophages promotes TNF-α secretion     

ZHANG Fang  LI Zi-ling  SHI Yi  ZHAO Ming  XIN Xiao-feng  QIAN Gui-sheng 《园艺学报》2007,23(7):1412-1414

AIM: To investigate the activation of nuclear factor κB (NF-κB) induced by lipopolysaccharides (LPS) in rat alveolar macrophages (AMs) and its regulatory role in tumor necrosis factor (TNF-α) secretion. METHODS: The dynamic activity changes of NF-κB induced by LPS were determined with electrophoretic mobility shift assay (EMSA). Antisense oligonucleotides of NF-κB subunit (p65) were transfected into AMs prior to LPS stimulation. The effect of antisense oligonucleotide transfection on expressions of p65 and TNF-α in supernatant were measured with Western blotting and enzyme linked immunosorbent assay (ELISA), respectively.RESULTS: NF-κB activity increased markedly and reached its peak level at 4 h after LPS stimulation. After transfected with antisense oligonucleotides of NF-κB subunit (p65), expression of p65 and TNF-α in supernatant decreased markedly.CONCLUSION: NF-κB activity has a positive effect on regulating secretion of TNF-α in AMs induced by LPS.  相似文献