Expression and distribution of monocarboxylate transporter 1 (MCT1) in the gastrointestinal tract of calves     

Kirat D  Inoue H  Iwano H  Hirayama K  Yokota H  Taniyama H  Kato S 《Research in veterinary science》2005,79(1):45-50

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Changes in Activities of Enzymes Related to Energy Metabolism in Peripheral Leukocytes of Fattening Steers     

Kimura N  Yoshimura I  Sako T  Inoue A  Tadami K  Arai T 《Veterinary research communications》2005,29(1):19-26

Glucose, triglyceride, cholesterol and immunoreactive insulin (IRI) concentrations, some enzyme activities in plasma, and activities of enzymes related to energy metabolism in peripheral leukocytes were measured in fattening Japanese Black Wagyu x Holstein steers fed on different diets at 8, 12, 16, 20 and 24 months of age. The plasma IRI concentrations at 20 and 24 months of age were significantly higher than those at 8 months of age. Activities of hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), aspartate aminotransferase (AST), and malate dehydrogenase (MDH) in cytosolic fractions, and glutamate dehydrogenase (GLDH), MDH and AST in mitochondrial fractions in peripheral leukocytes of steers at 24 months of age were significantly higher than those at 8 months. Increasing plasma insulin concentration was considered to induce acceleration of glucose utilization in leukocytes of fattening steers. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity in leukocytes increased significantly in the fattening process and was considered to be a useful indicator for evaluating changes in energy metabolism in steers.  相似文献   

Changes in expression and localization of connexin 43 mRNA and protein in porcine ovary granulosa cells during follicular atresia   总被引：2，自引：0，他引：2  

Cheng Y  Inoue N  Matsuda-Minehata F  Goto Y  Maeda A  Manabe N 《The Journal of reproduction and development》2005,51(5):627-637

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Insulin-like growth factor-I stimulated DNA replication in mouse endometrial stromal cells     

Inoue A  Takeuchi S  Takahashi S 《The Journal of reproduction and development》2005,51(3):305-313

Much evidence has suggested that sex steroid hormone-induced growth of uterine cells is mediated by polypeptide growth factors synthesized in uterine tissues. The present study aimed to clarify the effect of insulin-like growth factor-I (IGF-I) on the proliferation of mouse endometrial stromal cells obtained from immature mice. IGF-I and IGF-I receptor (type I) mRNAs were detected in the endometrial stromal cells. IGF-I increased bromodeoxyuridine (BrdU) uptake in the endometrial stromal cells, indicating an increase in DNA replication. E2 increased IGF-I mRNA levels in the endometrial stromal cells. IGF-I receptor is a tyrosine kinase receptor, and treatment with genistein, a tyrosine kinase inhibitor, reduced IGF-I-induced BrdU-uptake in the endometrial stromal cells. IGF-I signaling pathways involve mitogen-activated protein (MAP) kinase and phosphatidylinositol-3 kinase (PI-3 kinase). Treatment with 10(-7) M of the MAP kinase inhibitor PD098059 and 10(-5) M of the PI-3 kinase inhibitor LY294002 decreased IGF-I-induced BrdU-uptake in the endometrial stromal cells. However, LY294002 (10(-5) M) also decreased the BrdU-uptake in the absence of IGF-I treatment. These results suggest that endometrial IGF-I is involved in the proliferation of endometrial stromal cells in a paracrine or autocrine manner, and that the MAP kinase pathway is involved in DNA replication of endometrial stromal cells.  相似文献   

Drug susceptibility of Haemophilus pleuropneumoniae strains isolated from pigs   总被引：1，自引：0，他引：1  

A Inoue  K Yamamoto  N Hirano  T Murakami 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(2):175-180

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Involvement of Phosphoglucose Isomerase in Pathogenicity of Xanthomonas oryzae pv. oryzae     

Tsuge S  Ochiai H  Inoue Y  Oku T  Tsuno K  Kaku H  Kubo Y 《Phytopathology》2004,94(5):478-483

ABSTRACT Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, was subjected to transposon mutagenesis to generate mutants defective in pathogenicity. A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf blight-resistant rice and tomato. Cloning and sequence analysis revealed that the transposon in 74M913 was inserted in a gene homologous to the phosphoglucose isomerase (pgi) gene of X. axonopodis pv. citri. Growth of the mutant in a synthetic medium containing fructose or xylose as a sole carbohydrate source was much reduced, indicating the transposon disrupted pgi function. The interaction between expression of pgi and hypersensitive response and pathogenicity (hrp) genes was investigated because we had demonstrated previously that expression of hrp genes of X. oryzae pv. oryzae is induced in a synthetic medium containing xylose. However, pgi and the hrp gene (hrcU) were expressed independently. This study suggests that PGI is involved in pathogenicity of X. oryzae pv. oryzae.  相似文献   

Molecular epidemiological survey of benign Theileria parasites of cattle in Japan: detection of a new type of major piroplasm surface protein gene     

Kim JY  Yokoyama N  Kumar S  Inoue N  Yamaguchi T  Sentoku S  Fujisaki K  Sugimoto C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):251-256

Benign Theileria species of cattle are found in most parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a maker for epidemiological and phylogenetical studies of benign Theileria species. Parasites with Ikeda- or Chitose-type MPSP genes are dominant in Japan, but we report here mixed infection cases of Theileria parasites with an additional MPSP type parasite infecting cattle in Abashiri District, Hokkaido. The MPSP gene sequence found in the additional type was closely related to MPSP genes of Theileria parasites found in Southeast Asian countries, including Thailand (Narathiwat) and Indonesia (Java). Theileria parasites from the blood sample were also distinguishable from the Ikeda or Chitose type parasites by the small subunit (SSU) rRNA gene sequence analysis, and they are grouped into the SSU rRNA types C/D found in Korea, North America, and Spain. The present finding of mixed infections of cattle with three different types of Theileria makes epidemiological feature of bovine theileriosis in Japan more complex. We have designed a set of primers specific to this MPSP type in order to conduct further epidemiological study.  相似文献   

Establishment and characterization of four canine melanoma cell lines     

Inoue K  Ohashi E  Kadosawa T  Hong SH  Matsunaga S  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1437-1440

Four canine melanoma cell lines were established from the subcutaneous, oral gingival and mucosal melanoma tissues at the primary and metastatic sites. These cell lines were designated as CMeC-1, CMeC-2, KMeC and LMeC. The cells were spindles in shape, similar to that of primary tumor cells. The doubling times of these cells ranged from 34.1 +/- 5.61 to 57.9 +/- 3.28 hr and their chromosome number ranged from 46 to 80. When transplanted into nude mice, CMeC-1 and LMeC produced tumors, whereas CMeC-2 and KMeC did not. The morphology of the tissue formed by xenotransplantation of these cells was similar to their primary tumors.  相似文献   

Porcine (Sus scrofa) cellular FLICE-like inhibitory protein (cFLIP): molecular cloning and comparison with the human and murine cFLIP     

Goto Y  Matsuda-Minehata F  Inoue N  Matsui T  Maeda A  Manabe N 《The Journal of reproduction and development》2004,50(5):549-555

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Real-space observation of helical spin order     

Uchida M  Onose Y  Matsui Y  Tokura Y 《Science (New York, N.Y.)》2006,311(5759):359-361

Helical spin order in magnetic materials has been investigated only in reciprocal space. We visualized the helical spin order and dynamics in a metal silicide in real space by means of Lorentz electron microscopy. The real space of the helical spin order proves to be much richer than that expected from the averaged structure; it exhibits a variety of magnetic defects similar to atomic dislocations in the crystal lattice. The application of magnetic fields allows us to directly observe the deformation processes of the helical spin order accompanied by nucleation, movement, and annihilation of the magnetic defects.  相似文献