Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds     

Saiko Sugawara  Toshihiko Ito  Sho Sato  Mari Yokoo  Yuki Mori  Kano Kasuga  Ikuo Kojima  Tomokazu Fukuda  Ken‐ichi Yamanaka  Miki Sakatani  Masashi Takahashi  Masayuki Kobayashi 《Animal Science Journal》2013,84(3):275-280

Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the proper development of bovine embryos. We recently determined the FGF4 gene sequence in eight cattle derived from three breeds and revealed a common nucleotide sequence of the structural gene encoding FGF4, which leads to the deletion and mutation of amino acid sequences in the mature FGF4 (Pro³²‐Leu²⁰⁶) compared with the sequence previously reported. In the present study, HisbFGF4, a 6× histidine‐tagged bovine FGF4 (Pro³²‐Leu²⁰⁶), was produced in Escherichia coli based on the validated nucleotide sequence and purified by heparin column chromatography. In primary bovine fibroblasts, HisbFGF4 showed significant mitogenic activity, whereas, intriguingly, the activity of a commercially available recombinant human FGF4 (Gly²⁵‐Leu²⁰⁶) produced in E. coli was weaker than that of HisbFGF4. In conclusion, the present study provides a simple method for the production of a bioactive bovine FGF4 derivative in E. coli utilizing its structural gene elucidated by us.  相似文献   

GH secretory responses to ghrelin and GHRH in growing and lactating dairy cattle     

Itoh F  Komatsu T  Yonai M  Sugino T  Kojima M  Kangawa K  Hasegawa Y  Terashima Y  Hodate K 《Domestic animal endocrinology》2005,28(1):34-45

Release of growth hormone (GH) is known to be regulated mainly by GH-releasing hormone (GHRH) and somatostatin (SRIF) secreted from the hypothalamus. A novel peripheral release-regulating hormone, ghrelin, was recently identified. In this study, differences of the GH secretory response to ghrelin and GHRH in growing and lactating dairy cattle were investigated and an alteration of plasma ghrelin levels was observed. The same amounts of ghrelin and GHRH (0.3 nmol/kg) were intravenously injected to suckling and weanling calves, early and mid-lactating cows and non-lactating cows. Plasma ghrelin levels were also determined in dairy cattle in various physiological conditions. The peak values of ghrelin-induced GH secretion were increased in early lactating cows compared to those in non-lactating cows. The relative responsiveness of GH secretion to ghrelin was also increased compared with that to GHRH in early lactating cows. GH secretory responses to GHRH were blunted in mature cows with and without lactation. Conversely, GHRH-induced GH secretory response was greater than that to ghrelin in calves, and also greater in calves than in mature cows. Plasma ghrelin concentrations were elevated in early lactating cows compared to those in non-lactating cows. Plasma GH concentrations were higher in suckling calves and early lactating cows compared with those in non-lactating cows. These results suggest that GHRH is an effective inducer of GH release in growing calves, and that the relative importance of ghrelin in contributing to the rise in plasma GH increases in early lactating cows.  相似文献   

Antimicrobial susceptibility of pathogenic Escherichia coli isolated from sick cattle and pigs in Japan     

Harada K  Asai T  Kojima A  Oda C  Ishihara K  Takahashi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):999-1003

We examined the 12 antimicrobial susceptibilities of 175 E. coli isolates from sick cattle and pigs by an agar dilution method. Resistance was found in 78.3% of isolates for oxytetracycline, 70.3% of isolates for dihydrostreptomycin, and 49.1% of isolates for ampicillin. When compared with healthy animals reported by Kijima-Tanaka et al., resistance rates for 11 antimicrobial agents were higher in sick cattle than in healthy cattle, and resistance rates for all antimicrobial agents were higher in sick pigs than in healthy pigs. Comparing cattle and pigs, resistance rates to colistin was higher in porcine isolates than in bovine isolates, but was lower in porcine isolates than in bovine isolates for cefazolin. With regard to the association of virulence factors, higher resistance rates to colistin and enrofloxacin were observed in STEC (61 strains) than in non-STEC (57 strains) among porcine isolates, while there were no significant differences in bovine isolates. In conclusion, these results can be considered helpful for adequate selection and prudent use of antimicrobial agents for several types of colibacillosis.  相似文献   

Male pronuclear formation by a boar spermatozoon with hairpin-curved tail in zona-free hamster egg     

M Yoshida  Y Kojima 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(2):428-430

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Hepatic fibrosis produced in rats by repeated intraperitoneal injections of swine serum     

K Kitamura  A Yasoshima  H O Iwasaki  A Kojima  K Doi  A Okaniwa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(3):265-271

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Morphological study of the abnormal spermatozoa of a bull using electron microscopy     

Y Kojima  T Ishikawa  Y Mifune 《The Japanese journal of veterinary research》1969,17(4):97-104

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Rapid identification and differentiation of pathogenic clostridia in gas gangrene by polymerase chain reaction based on the 16S-23S rDNA spacer region     

Sasaki Y  Yamamoto K  Kojima A  Norimatsu M  Tamura Y 《Research in veterinary science》2000,69(3):289-294

In cattle, sheep, and other ruminants, clostridial myonecrosis (gas gangrene) is mostly caused by Clostridium chauvoei, C septicum, C novyi and C sordellii. A polymerase chain reaction (PCR) system using common primers designed from multiple alignment of the 16S rRNA and 23S rRNA genes of Clostridium species was developed to identify pathogenic clostridia. The PCR was performed with total DNA from 26 strains which included seven different Clostridia species. These bacteria were differentiated at species level by the different PCR product patterns. To characterise the 16S-23S rDNA spacer regions of these clostridia further, most PCR products of these bacteria were sequenced. The smallest PCR products of each bacterium represented the fundamental 16S-23S rDNA spacer region; larger PCR products of each bacterium were caused by insertion sequences, i.e. tRNA gene sequences. The authors' observations indicate that the PCR patterns of the 16S-23S rDNA spacer regions have the potential to be used as an identification marker of pathogenic clostridia in gas gangrene.  相似文献   

Electron microscopic study of the bull spermatozoon. VI-VII     

Y Kojima 《The Japanese journal of veterinary research》1966,14(1):51-62

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Strain differences in the spontaneous incidence of sperm morphological abnormalities in Hatano rats     

Sato M  Ohta R  Kojima K  Shirota M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(4):389-390

Two inbred strains of Hatano rats, which had been bred selectively on the basis for shuttlebox avoidance responses (high- and low-avoidance animals; HAA and LAA), were different in sperm motility. We have found that there are strain differences in the spontaneous incidence of sperm morphological abnormalities. The percentages of abnormalities were extraordinarily higher in the LAA rats, whose sperm motility was lesser. Since the high sperm abnormality was an exceptional case in rats, these rats may be useful models for sperm quality.  相似文献   

Hepatic copper accumulation in a young cat with familial variations in the ATP7B gene     

Hajime Asada  Mari Kojima  Takuro Nagahara  Yuko Goto‐Koshino  James K. Chambers  Taisuke Nakagawa  Nozomu Yokoyama  Kazuyuki Uchida  Hajime Tsujimoto  Koichi Ohno 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):874-878

A 9‐month‐old intact crossbred female cat was presented with jaundice, intermittent anorexia and lethargy, increased hepatic enzyme activities, and hyperammonemia. Abdominal ultrasound and computed tomographic examinations determined that the liver had a rounded and irregular margin, and histopathological examination identified excessive accumulation of copper hepatocytes in the liver. Concentrations of both blood and urine copper were higher than in healthy cats. The patient responded well to treatment with penicillamine. Clinicopathological abnormalities and clinical signs improved within 2 months, and the patient was alive for >9 months after starting treatment. Genetic examination determined that the patient and its littermate had a single‐nucleotide variation (SNV, p. T1297R) that impaired the function of the ATP7B gene product; the gene that is mutated in patients with Wilson's disease (WD). Hepatic copper accumulation was believed to be associated with the SNV of the ATP7B gene, and the patient had a genetic disorder of copper metabolism equivalent to WD in humans.  相似文献