Comparison of Survival Times for Dogs with Pituitary‐Dependent Hyperadrenocorticism in a Primary‐Care Hospital: Treated with Trilostane versus Untreated          下载免费PDF全文

N. Nagata  K. Kojima  M. Yuki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(1):22-28

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Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts          下载免费PDF全文

Tomohide Takaya  Yuma Nihashi  Shotaro Kojima  Tamao Ono  Hiroshi Kagami 《Animal Science Journal》2017,88(11):1880-1885

Cell‐cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB‐GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB‐DsRed. mMB‐GFP and chMB‐DsRed were cocultured and induced to differentiate. After 24 h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP⁺/DsRed⁺ hybrid myotubes were able to survive and grew to hyper‐multinucleated mature form. We also found that undifferentiated mMB‐GFP efficiently fuse to the chMB‐DsRed‐derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast‐based fusogenic technique will open up an alternative direction to create novel hybrid products.  相似文献   

Effect of accelerated aging treatment on a surface property and dynamic mechanical properties of commercial wood-based panels     

Sahriyanti Saad  Hikaru Kobori  Yoichi Kojima  Shigehiko Suzuki 《Journal of Wood Science》2017,63(5):496-505

Changes in surface property and mechanical properties in commercial particleboards and medium density fiberboards subjected to repetitive relative humidity treatment (i.e., aging treatment) were investigated. The stylus technique was used to evaluate surface roughness and a non-destructive vibrational test was used to evaluate dynamic bending strength during aging treatment. These methods evaluated the effect of aging treatment effectively. The aging treatment increased surface roughness and loss tangent (tan δ) values, but decreased dynamic modulus of elasticity (E _d) values of the panels compared to the respective initial values. The increment of surface roughness and E _d degradations observed were larger than the tan δ degradations. Surface roughness and tan δ seemed to be relatively dependent on resin type, whereas E _d was relatively independent under the aging treatment. Overall, the medium density fiberboard bonded methylene diphenyl diisocyanate (MDF(MDI)) panel was superior among the types of panels studied under the aging treatment. There was strong correlation between average roughness (R _a) increment and E _d retention.  相似文献   

An extraskeletal osteosarcoma in an aged rat     

Y Minato  T Yamamura  H Takada  A Kojima  K Imaizumi  I Wada  M Takeshita  A Okaniwa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(1):259-261

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Multiple headed spermatozoa in the boar testes     

Y Kojima  Y Kinoshita  M Fukuda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1974,36(5):364-374

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Antimicrobial susceptibility of Mannheimia haemolytica isolates from cattle in Japan from 2001 to 2002     

Esaki H  Asai T  Kojima A  Ishihara K  Morioka A  Tamura Y  Takahashi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):75-77

A total of 27 clinical isolates of Mannheimia haemolytica from cattle in Japan from 2001 to 2002 were examined for antimicrobial susceptibility to 25 antimicrobial agents. The minimum inhibitory concentrations of 25 different antimicrobials were determined by an agar dilution method according to the guidelines of the National Committee for Clinical Laboratory Standards. Of the 27 isolates, seven isolates (26.9%) were resistant to at least one of the 25 drugs and resistance rates ranged from 3.7 to 18.5%. Resistance rates to dihydrostreptomycin (18.5%), oxytetracycline (11.1%), and doxycycline (11.1%) were relatively high and those to the remaining drugs were less than 10%.  相似文献   

In vitro activity of 24 antimicrobial agents against Staphylococcus and Streptococcus isolated from diseased animals in Japan     

Morioka A  Asai T  Ishihara K  Kojima A  Tamura Y  Takahashi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(2):207-210

A total of 88 Staphylococcus and 61 Streptococcus isolates from diseased animals throughout Japan were examined in 2000 for the minimum inhibitory concentrations of 24 different antimicrobials by the agar dilution method standardized by the Japanese Society of Chemotherapy. The resistance rates to aminobenzylpenicillin (36.4%) and benzylpenicillin (35.2%) were high in Staphylococcus isolates, and those to oxytetracycline (45.9%) and kanamycin (21.3%) were high in Streptococcus isolates. Two isolates resistant to oxacillin harbored the mecA gene. One was Staphylococcus epidermidis derived from a pig with arthritis, and the other Staphylococcus cohnii from a head of cattle with mastitis.  相似文献   

Effect of an orally active progestin on follicular dynamics in cycling and anestrous postpartum beef cows     

Perry GA  Kojima FN  Salfen BE  Bader JF  Patterson DJ  Smith MF 《Journal of animal science》2002,80(7):1932-1938

Although treatment of cycling cows with low concentrations of melengesterol acetate (MGA) results in formation of persistent follicles, in the absence of corpora lutea, it is not known whether persistent follicles form in anestrous cows in response to a similar treatment. The objective of this experiment was to determine the effect of long-term MGA treatment (14 d) on follicular dynamics and the secretion of estradiol in anestrous postpartum beef cows. Treatment groups (replicated over 2 yr) included the following: anestrous control (AC; n = 11), anestrous MGA (AM; n = 16), and cycling MGA (CM; positive control; n = 16). Angus-crossbred cows were assigned to treatment by age, cow body condition, and days postpartum. Cows were fed carrier (AC group) or 0.5 mg MGA x animal(-1) x d(-1) (AM and CM groups) for 14 d beginning approximately 38 d postpartum. Cows allotted to the CM group were injected with PGF2alpha, on the first day of MGA treatment to induce luteolysis. The preceding treatment (CM) results in formation of persistent follicles and secretion of elevated concentrations of estradiol. Ovaries of each cow were examined daily by transrectal ultrasonography beginning 5 to 7 d preceding the initiation of feeding MGA or carrier and continued until ovulation or 7 d following MGA feeding. There was no difference among groups in the stage of follicular wave or diameter of the largest follicle at the start of carrier or MGA feeding. The length of the follicular wave present at the start of MGA feeding was greater (P < 0.01) for cows in the CM (14.5 d, yr 1; 18.3 d, yr 2) group compared to the AM (9.4 d, yr 1; 7.9 d, yr 2) or AC (9.7 d, yr 1; 10.7 d, yr 2) groups. Maximum follicular diameter over both years was greater (P < 0.01) for the CM (20.6 mm) group than the AM (15.1 mm) or AC (16.4 mm) groups. Circulating concentrations of estradiol were also increased (P < 0.05) in the CM group compared to the AM or AC groups. However, MGA appeared to have no effect (P > 0.05) on the number of follicles recruited, growth rate of the dominant follicle during the first 6 d oftreatment, or growth rate to the maximum follicular diameter. In summary, MGA treatment did not increase the duration ot the follicular wave, maximum follicular diameter, or secretion of estradiol in anestrous postpartum cows, nor did MGA affect the number of follicles recruited or growth rate of dominant follicles in cycling or anestrous animals.  相似文献   

Characterization of Staphylococcus aureus strains isolated from bovine mastitis in Japan     

Nagase N  Shimizu A  Kawano J  Yamashita K  Yoshimura H  Ishimaru M  Kojima A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1169-1172

Fifty-three strains of Staphylococcus aureus isolated from cows affected with mastitis from 21 prefectures in Japan were characterized by phenotypic and genotypic methods. Thirty-three (62.3%) strains showed biotype K-beta+CV:A, coagulase type VI, and sensitivity to bovine phages of group III or IV. These 33 strains could be subdivided into two groups on the basis of the production of staphylococcal enterotoxins (SEs) and on toxic shock syndrome toxin-1 (TSST-1). By pulsed-field gel electrophoresis, the 16 SEC- and TSST-1-producing strains showed similar patterns that differed by only a few fragments, suggesting that they were genetically closely related. Fifteen of 17 non SEC-producing strains which did not produce any other SEs and TSST-1 were genetically different from the SEC-producing strains and showed genetic diversity.  相似文献   

Antimicrobial susceptibility of Arcanobacterium pyogenes isolated from cattle and pigs     

Yoshimura H  Kojima A  Ishimaru M 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(2):139-143

The minimum inhibitory concentrations (MICs) of 18 antimicrobial agents were determined for 49 Arcanobacterium pyogenes isolates (42 bovine isolates and 7 porcine isolates). Benzylpenicillin and ampicillin were the most active antibiotics, with MIC ranges of < or = 0.0125-0.05 microgram/ml for both bovine and porcine isolates. All isolates were susceptible to penicillins and cephems. MICs for 90% of the isolates of dihydrostreptomycin, gentamicin and oxytetracycline for bovine isolates were > 100 micrograms/ml, 1.56 micrograms/ml and 25 micrograms/ml, respectively. More resistance to dihydrostreptomycin appeared among porcine isolates (85.7%) than among bovine isolates (52.4%). Resistance to gentamicin occurred in only 3 (7.1%) of the bovine isolates. Resistance to oxytetracycline also appeared more frequent among porcine isolates (85.7%) than among bovine isolates (57.1%). All bovine isolates were susceptible to erythromycin, tilmocosin and lincomycin, but two porcine isolates (28.6%) were simultaneously resistant to these antibiotics. Tiamulin was as active as tilmicosin, with an MIC for 50% of the isolates (MIC50) of 0.05 microgram/ml for both bovine and porcine isolates. The MIC50s of chloramphenicol and its derivatives florfenicol and thiamphenicol were all 1.56 micrograms/ml. The fluoroquinolones enrofloxacin and ofloxacin were not so active as penicillins and macrolides, with MIC50s of 0.78 microgram/ml and 1.56 micrograms/ml, respectively, for both bovine and porcine isolates.  相似文献