大豆卵磷脂研究现状     

孟凡钢  杨振宇  闫日红  董岭超  姜兆生  王立辉  赵久安 《大豆科技》2013,(2):40-44

大豆在我国有广泛的种植,大豆卵磷脂是大豆油生产过程中的副产品,是一种理想的多用途天然原料,具有十分独特的功能和作用,并已在许多领域内得到广泛的应用。介绍大豆卵磷脂的化学结构、理化性质、应用、提纯方法等方面的研究概况,并对此进行了展望。  相似文献   

RGO/Nylon-6 composite mat with unique structural features and electrical properties obtained from electrospinning and hydrothermal process     

Hem Raj Pant  Bishweshwar Pant  Chan Hee Park  Han Joo Kim  Dong Su Lee  Leonard D. Tijing  Bo Sang Hwang  Hak Yong Kim  Cheol Sang Kim 《Fibers and Polymers》2013,14(6):970-975

In this work, the reduced graphene oxide (RGO) sheets were effectively uploaded through nylon-6 fibers using combined process of electrospinning and hydrothermal treatment. Good dispersion of graphene oxide (GO) with nylon-6 solution could allow to upload GO sheets through nylon-6 fibers and facilitate the formation of spider-wave-like nano-nets during electrospinning. GO sheets present on/into nylon-6 spider-wave-like nano-nets were further reduced to RGO using hydrothermal treatment. The impregnated GO sheets into nylon-6 nanofibers and their reduction during hydrothermal treatment were confirmed by FE-SEM, TEM, FT-IR and Raman spectra. The electrical characteristics of pristine nylon-6, GO/nylon-6 and RGO/nylon-6 nanofibers were investigated and it was found that RGO/nylon-6 composite mat had better electrical conductivity than others. The formation of spider-wave-like nano-nets as well as indirect route of incorporation of RGO sheets on electrospun nylon-6 mat may open a new direction for future graphene/polymer electronics.  相似文献   

Utilization of fruit by-products for organic reducing agent in indigo dyeing     

Younsook Shin  Min Choi  Dong Il Yoo 《Fibers and Polymers》2013,14(12):2027-2031

This study aimed to investigate the efficacy of banana peel extract as a possible substitute for the environmentally unfavorable sodium dithionite in cellulosic fiber dyeing with indigo. Antioxidant activity and total sugar content of the banana peel extract were measured. Composition of the extract was investigated by GC/MS analysis. Its reducing power for synthetic indigo was evaluated by reduction potential measurements and dyeing tests. It was shown that the studied extract could be applied successfully in the reduction of indigo in spite of their lower reducing power when compared with that of sodium dithionite. In dyeing study, sodium dithionite showed its superiority in term of reducing power over the studied extract but the studied extract was superior stability of reduction to sodium dithionite. So, it can be concluded that banana peel extract could be a biodegradable and sustainable alternative to sodium dithionite as a reducing agent in indigo dyeing processes.  相似文献   

氟喹诺酮类药物多组分残留检测抗体的筛选     

魏东  张乃生 《兽医大学学报》2013,(11):1715-1719

通过N-羟基琥珀酰亚胺活性酯法将诺氟沙星、环丙沙星、达氟沙星和沙拉沙星分别与牛血清白蛋白偶联制备免疫抗原,与卵清白蛋白偶联制备检测抗原,筛选一种可用于多种氟喹诺酮残留的检测抗体。将免疫抗原分别免疫獭兔制备多克隆抗体,采用间接竞争ELISA法测定抗体特异性。诺氟沙星抗体特异性较强,与同类药物的交叉反应较少,沙拉沙星抗体与同类药物发生交叉反应最多,且与诺氟沙星、环丙沙星和恩诺沙星的交叉反应率较高。结果表明,确定沙拉沙星抗体作为进一步研究的抗体。  相似文献   

我国部分地区送检样品3种常见猪病血清学和病原学检测     

梁俊超  王辉之  刘晓东  刘红祥  李坤  孔宪好  马振乾  胡继明  刘东  于录 《中国动物检疫》2020,37(10):21-26

为了解我国部分地区猪瘟、猪繁殖与呼吸综合征和猪伪狂犬病3种常见疫病的流行情况,采用ELISA、聚合酶链式反应（PCR）和反转录PCR（RT-PCR）等方法,对2019年11个省（自治区）172个猪场送检的3 550份猪血清样品进行猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）及伪狂犬病野毒（PRV-gE）抗体检测,对9个省（自治区）72个猪场送检的371份病料进行抗原检测。结果显示：CSFV、PRRSV、PRV-gE抗体阳性率分别为82.75%、84.08%、31.86%,抗原阳性率分别为2.62%、26.15%、1.67%。保育猪群CSFV抗体平均阻断率最低,为44.62%,抗体整齐度差,CV为58.26%,且CSFV抗原主要在哺乳仔猪和保育猪病料中被检出,检出率分别为3.57%、3.00%;除育肥猪群外,其他各阶段猪群的PRRSV抗体S/P平均值为1.0~2.0,抗体整齐度整体较差,且平均抗原阳性率较高（26.15%）,其中保育阶段猪群病原检出率最高（37.68%）;育肥猪、母猪、公猪的PRV-gE抗体阳性率分别为36.82%、35.31%、15.65%,病原主要从死胎/木乃伊胎和哺乳仔猪病料中被检出,检出率分别为8.70%和2.99%。结果表明,PRRSV对我国猪场不同阶段猪群均有较大威胁;保育猪群CSFV抗体水平不理想,暴发疫情风险较高;公猪、母猪及育肥猪群普遍存在PRV感染,尤其是母猪。提议各猪场采取免疫、监测、净化等综合措施,加强这3类常见猪群疫病的防控。  相似文献   

Amino acid substitutions in gyrA and parC associated with quinolone resistance in nalidixic acid-resistant Salmonella isolates     

Dong Hwa Bae  Ho Jeong Baek  So Jeong Jeong  Young Ju Lee 《Irish veterinary journal》2013,66(1):23

This study was undertaken to identify and characterize amino acid substitutions in gyrA and parC related with quinolone resistance of 27 nalidixic acid-resistant (Na^R) Salmonella isolates collected in poultry slaughterhouses in Korea. A total of 51 Salmonella isolates were detected from 44.8% (47/105) of the total samples from 15 poultry slaughterhouses examined, among which 27 (52.9%) Na^R isolates were detected while ciprofloxacin (Cip) resistance was not present in the isolates. These 27 Na^R isolates of DNA sequencing revealed that it contained three types of gyrA mutations in only D87 codon. Mutations in the D87 codon resulted in substitutions to G in most of the isolates, but D87Y and D87N exchanges were also detected. Although Cip resistance was absent, reduced susceptibility characterized by mutations in gyrA was apparent among Salmonella isolates from poultry slaughterhouses in Korea.  相似文献   

H9N2亚型禽流感流行分析及疫苗毒株的筛选     

刘东  宫晓  刘晓东  梁俊超  师东方 《中国动物检疫》2013,30(6):45-50

本研究旨在筛选新型免疫原性好、广谱的优秀 H9亚型禽流感病毒疫苗株。通过 H9N2分子流行病学调查对分离得到的37株禽流感 H9N2亚型病毒进行 HA 基因分子遗传进化及其关键位点分析,利用交叉 HI 效价及鸡胚中和试验分析毒株间毒力及其相关性,制备疫苗用毒株进行免疫攻毒保护试验。结果显示,从37株病毒中选出12株病毒作为代表,分为3个分支,同分支之间交叉 HI 效价及中和效价最高。HI 试验及鸡胚中和试验均显示同分支毒株间的相关系数高。免疫攻毒保护试验显示同分支疫苗株安全性高。表明我国禽流感 H9N2亚型情况复杂,且疫苗保护效果与疫苗株的抗原匹配性密切相关,生产多种分支 H9疫苗株联合更有利于控制 H9型禽流感,  相似文献   

Cytotoxic and Antibacterial Cembranoids from a South China Sea Soft Coral,Lobophytum sp     

Min Zhao  Jian Yin  Wei Jiang  Minshan Ma  Xinxiang Lei  Zheng Xiang  Jianyong Dong  Kexin Huang  Pengcheng Yan 《Marine drugs》2013,11(4):1162-1172

Chemical examination of a South China Sea soft coral Lobophytum sp. led to the isolation of three new α-methylene-γ-lactone-containing cembranoids, (1R*,3R*,4R*,14R*,7E,11E)-3,4-epoxycembra-7,11,15(17)-trien-16,14-olide (1), (1R*,7S*,14S*,3E,11E)-7-hydroperoxycembra-3,8(19),11,15(17)-tetraen-16,14-olide (2), and (1R*,7S*,14S*,3E,11E)-18-acetoxy-7-hydroperoxycembra-3,8(19),11,15(17)-tetraen-16,14-olide (3), along with eleven known analogues 4–14. The structures of the new compounds were elucidated through extensive spectroscopic analysis, including 1D and 2D NMR data. Compounds 1–3 exhibited moderate cytotoxic activity against the selected tumor cell lines. Moreover, 2 and 3 were found to be moderate inhibitors against the bacteria S. aureus and S. pneumoniae.  相似文献   

Invasion biology of spotted wing Drosophila (Drosophila suzukii): a global perspective and future priorities     

Mark K. Asplen  Gianfranco Anfora  Antonio Biondi  Deuk-Soo Choi  Dong Chu  Kent M. Daane  Patricia Gibert  Andrew P. Gutierrez  Kim A. Hoelmer  William D. Hutchison  Rufus Isaacs  Zhi-Lin Jiang  Zsolt Kárpáti  Masahito T. Kimura  Marta Pascual  Christopher R. Philips  Christophe Plantamp  Luigi Ponti  Gábor Vétek  Heidrun Vogt  Vaughn M. Walton  Yi Yu  Lucia Zappalà  Nicolas Desneux 《Journal of pest science》2015,88(3):469-494

  相似文献   

Effects of water deficit and high N fertilization on wheat storage protein synthesis,gluten secondary structure,and breadmaking quality     

Junxian Liu  Junwei Zhang  Gengrui Zhu  Dong Zhu  Yueming Yan 《作物学报(英文版)》2022,(1):216-223

The content and composition of wheat storage proteins are the major determinants of dough rheological properties and breadmaking quality and are influenced by cultivation conditions.This study aimed to investigate the effects of water deficit and high N-fertilizer application on wheat storage protein synthesis,gluten secondary structure,and breadmaking quality.Reverse-phase ultrahigh-performance liquid chromatography analysis showed that storage protein and gluten macropolymer accumulation was promoted under both independent applications and a combination of water-deficit and high N-fertilizer treatments.Fourier-transform infrared spectroscopy showed that water deficit and high N-fertilizer treatments generally improved protein secondary structure formation and lipid accumulation,and reduced flour moisture.In particular,high N-fertilizer application increasedβ-sheet content by 10.4%and the combination of water-deficit and high N-fertilizer treatments increased random coil content by 7.6%.These changes in gluten content and secondary structure led to improved dough rheological properties and breadmaking quality,including superior loaf internal structure,volume,and score.Our results demonstrate that moderately high N-fertilizer application under drought conditions can improve gluten accumulation,gluten secondary structure formation,and baking quality.  相似文献