Genetic analysis and molecular mapping of QTLs associated with resistance to bacterial blight in a rice mutant,SA0423     

Hsin-Yi Tseng  Da-Gin Lin  Hsiao-Ying Hsieh  Ya-June Tseng  Wen-Bin Tseng  Chun-Wei Chen  Chang-Sheng Wang 《Euphytica》2015,205(1):231-241

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Radiation induced variations in photoperiod-sensitivity,thermo-sensitivity and the number of days to heading in rice     

S. C. Hsieh  T. M. Chang 《Euphytica》1975,24(2):487-496

Summary Radiation induced semi-dwarf mutants derived from five japonica type varieties of rice were studied with regard to their photoperiod-sensitivity, thermo-sensitivity and the number of days to heading. The experiment was carried out under the natural conditions at Taipei. The coefficient of photoperiod-sensitivity (T.D.M.) and thermo-sensitivity (byx. z and byz. x) as developed by Oka (1954) were estimated for the mutants in comparison with their original varieties.It was observed that these various physiological characters could be altered rather easily by mutations. Mutants showed wider ranges in both positive and negative directions than their original varieties in all the physiological characters studied. Even though heading date depends on both photoperiod-sensitivity and thermo-sensitivity, it was estimated which of the two contributed more to the induced earliness in each mutant. This offers a basis for selecting early maturing lines of rice.Taiwan Agricultural Research Institute, Taipei, Taiwan, Republic of China.  相似文献   

Isolation and gas chromatographic determination of chlorsulfuron in milk   总被引：2，自引：0，他引：2  

A R Long  L C Hsieh  M S Malbrough  C R Short  S A Barker 《Journal of the Association of Official Analytical Chemists》1989,72(5):813-815

A method for the isolation and gas chromatographic determination of chlorsulfuron in milk is presented. Blank or chlorsulfuron-spiked milk samples were blended into C-18 (octadecylsilyl derivatized silica, ODS) packing material. A column made from the C-18/milk matrix was washed with hexane after which chlorsulfuron was eluted with dichloromethane (DCM). The DCM eluate contained chlorsulfuron which was free from interfering co-extractants when analyzed by gas chromatography utilizing a nitrogen/phosphorus detector. Chlorsulfuron was found to undergo a thermally induced decomposition to give 2-amino-4-methoxy-6-methyl-1,3,5-triazine, which was detected and quantitated by this method. Standard curves for these analyses were linear (r = 0.992 +/- 0.004, n = 5), with an average percentage recovery of 91.6 +/- 10.8%, over the concentration range examined (62.5-2000 ng/mL). The inter- and intra-assay variabilities were 11.6 +/- 7.5% and 6.2%, respectively.  相似文献   

Analysis of phosphorus-containing hydrolytic products of organophosphorus insecticides in water.     

C G Daughton  D G Crosby  R L Garnas  D P Hsieh 《Journal of agricultural and food chemistry》1976,24(2):236-241

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selective production and characterization of levan by Bacillus subtilis (Natto) Takahashi   总被引：2，自引：0，他引：2  

Shih IL  Yu YT  Shieh CJ  Hsieh CY 《Journal of agricultural and food chemistry》2005,53(21):8211-8215

To meet the industrial need of an efficient microbial method for increased levan production, Bacillus subtilis (natto) Takahashi, a commercial natto starter for preparing fermented soybeans (natto), was used to produce levan. After cultivation for 21 h, 40-50 mg of levan mL(-1) was produced in medium containing 20% (w/w) sucrose, which was approximately 50% yield on available fructose. The product consisted of two fractions with different molecular masses (1794 and 11 kDa), which were easily separated by fractionation using an ethanol gradient. The products were well characterized by GPC, 13C NMR, and 1H NMR. The various sugars and concentrations, initial pH, fermentation temperature, and agitation speed affected the levan production by B. subtilis (natto) Takahashi. Takahashi strain is the most efficient levan-producing strain among all of the B. subtilis strains tested and, as previously reported, it produced the highest yield of levan in the least time (21 h) under the common cultivation condition.  相似文献   

Aqueous extract of Monascus purpureus M9011 prevents and reverses fructose-induced hypertension in rats     

Hsieh PS  Tai YH 《Journal of agricultural and food chemistry》2003,51(14):3945-3950

This study aimed to determine the antihypertensive and metabolic effects of an aqueous extract of Monascus purpureus M9011 on fructose-induced hypertensive rats. After dietary feeding of fructose for 2 weeks, the rats exhibited significantly higher systolic blood pressure (SBP), mean arterial pressure (MAP), and plasma insulin and triglyceride levels, but lower insulin sensitivity than those in control rats on regular diet. The intragastric loading of fructose-fed rats with M9011 containing gamma-aminobutyric acid (GABA, 1 mg.kg(-)(1).day(-)(1)) prevented the development of fructose-induced hypertension. After fructose-induced hypertension had been established, intragastric loading of M9011 reversed the elevated blood pressure to normal level. Administration of pure GABA at the same dose as that contained in M9011 failed to prevent or reverse hypertension due to fructose consumption. Chronic M9011 treatment significantly suppressed the fructose-induced elevation in total cholesterol levels and enhanced the recovery of high-density lipoprotein cholesterol/total cholesterol ratio. However, M9011 treatment did not alter insulin sensitivity or the plasma levels of insulin, glucose, and triglyceride in fructose-fed and control rats. The present results suggest that M9011 is a novel, potent, food-based antihypertensive agent with the capability to improve long-term control of cholesterol metabolism in rats and may be of importance in clinical application for the hypertensive diabetic population.  相似文献   

Effects of processing conditions on the physical and chemical properties of buckwheat grit cakes     

Im JS  Huff HE  Hsieh FH 《Journal of agricultural and food chemistry》2003,51(3):659-666

Buckwheat grit cakes were prepared with a rice cake machine using the following independent variables: tempering moisture contents (15, 17, and 19%, wb), heating temperatures (240, 246, 252, and 258 degrees C), and heating times (5, 6, 7, and 8 s). Higher moisture, higher heating temperature, or longer heating time produced cakes with a higher cake specific volume. Cakes became lighter in color at a lower heating temperature or a shorter heating time. The hardest cake was produced at 252 degrees C for 5 s at 19% moisture content. The percent weight loss after tumbling decreased with increasing heating times and heating temperatures. Increased heating time resulted in more expanded products. The average rutin content decreased as the heating temperature or heating time increased. These results suggest that processing conditions, including tempering moisture, heating temperature, and heating time, significantly influenced physical and chemical qualities of buckwheat grit cakes such as specific volume, hardness, integrity, color, internal structure, and rutin content.  相似文献   

Matrix solid phase dispersion (MSPD) isolation and liquid chromatographic determination of furazolidone in pork muscle tissue     

A R Long  L C Hsieh  M S Malbrough  C R Short  S A Barker 《Journal of the Association of Official Analytical Chemists》1991,74(2):292-294

A method for the isolation and liquid chromatographic (LC) determination of furazolidone in pork muscle tissue is presented. Blank or furazolidone-fortified pork muscle tissue samples (0.5 g) were blended with octadecylsilyl (C18, 18% load, endcapped, 2 g) derivatized silica. A column made from C18/pork matrix was first washed with hexane (8 mL), followed by elution of furazolidone with ethyl acetate. The ethyl acetate extract was then passed through an activated alumina column. The eluate contained furazolidone that was free from interfering compounds when analyzed by LC with UV detection (photodiode array, 365 nm). Detector response with increasing concentrations of furazolidone isolated from fortified samples was linear (r = 0.998 +/- 0.002) with an average percentage recovery of 89.5 +/- 8.1% for the concentration range (7.8-250 ng/g) examined and resulted in a minimum detectable limit of 390 pg on column, and a detector response of more than 5 times baseline noise. The inter-assay variability was 9.9 +/- 5.4% with an intra-assay variability of 1.5%.  相似文献   

Solar wind-induced atmospheric erosion at Mars: first results from ASPERA-3 on Mars Express     

Lundin R  Barabash S  Andersson H  Holmström M  Grigoriev A  Yamauchi M  Sauvaud JA  Fedorov A  Budnik E  Thocaven JJ  Winningham D  Frahm R  Scherrer J  Sharber J  Asamura K  Hayakawa H  Coates A  Linder DR  Curtis C  Hsieh KC  Sandel BR  Grande M  Carter M  Reading DH  Koskinen H  Kallio E  Riihela P  Schmidt W  Säles T  Kozyra J  Krupp N  Woch J  Luhmann J  McKenna-Lawler S  Cerulli-Irelli R  Orsini S  Maggi M  Mura A  Milillo A  Roelof E  Williams D  Livi S  Brandt P  Wurz P  Bochsler P 《Science (New York, N.Y.)》2004,305(5692):1933-1936

The Analyzer of Space Plasma and Energetic Atoms (ASPERA) on board the Mars Express spacecraft found that solar wind plasma and accelerated ionospheric ions may be observed all the way down to the Mars Express pericenter of 270 kilometers above the dayside planetary surface. This is very deep in the ionosphere, implying direct exposure of the martian topside atmosphere to solar wind plasma forcing. The low-altitude penetration of solar wind plasma and the energization of ionospheric plasma may be due to solar wind irregularities or perturbations, to magnetic anomalies at Mars, or both.  相似文献   

Isolation and identification of cucurbitane-type triterpenoids with partial agonist/antagonist potential for estrogen receptors from Momordica charantia     

Hsu C  Hsieh CL  Kuo YH  Huang CJ 《Journal of agricultural and food chemistry》2011,59(9):4553-4561

This study aims at investigating the estrogenic activity and active cucurbitane-type triterpenoid compounds of bitter gourd (Momordica charantia, MC) using a transactivation assay for estrogen receptors (ER) α and β. The lyophilized fruits of MC were exhaustively extracted with ethyl acetate (EA) and 95% ethanol (EtOH), sequentially. The nonsaponifiable fraction (NS) of the EA extract as well as the acid hydrolyzed EtOH extract (AH) was fractionated and isolated by repeated column chromatography and further purified by preparative HPLC or RP-HPLC. One known compound, 5β,19-epoxycucurbita-6,24-diene-3β,23ξ-diol (6), was isolated from the NS, and five new compounds (1-5) were isolated from AH and identified as cucurbita-6,22(E),24-trien-3β-ol-19,5β-olide (1), 5β,19-epoxycucurbita-6,22(E),24-triene-3β,19-diol (2), 3β-hydroxycucurbita-5(10),6,22(E),24-tetraen-19-al (3), 19-dimethoxycucurbita-5(10),6,22(E),24-tetraen-3β-ol (4), and 19-nor-cucurbita-5(10),6,8,22(E),24-pentaen-3β-ol (5). In the noncytotoxic concentration range, compounds 1, 2, 5 and 6 showed weak agonistic activity via ER α and β. Compounds 1, 2, 3 and 6 significantly antagonized the transactvation of 17β-estradiol (E(2)) via both ER α and β. In conclusion, this study demonstrates, for the first time as far as we know, the partial agonist/antagonist activity via ER of four new and one known cucurbitane-type triterpenoids from MC. Further studies are worthy to explore the selective estrogen receptor modulator (SERM) activity of MC.  相似文献