The complete nucleotide sequences of L3 and S7 segments of Ibaraki virus encoding for the major inner capsid proteins, VP3 and VP7   总被引：2，自引：0，他引：2  

Iwata H  Manabe S  Yoshida A  Pereira EM  Inoue T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):73-78

The complete nucleotide sequences of the genes encoding two of the major inner capsid proteins of Ibaraki virus (IBAV), belonging to epizootic hemorrhagic disease virus serotype 2 (EHDV-2) were determined. The L3 RNA segment is 2768 nucleotides in length which encodes VP3 polypeptides of 899 amino acid residues (M.W. 103 kDa). The S7 RNA segment, which encodes the VP7 core protein, is 1162 nucleotides in length and encodes 349 amino acids (M.W. 38 kDa). These RNA segments had the characteristic consensus motifs of Orbivirus RNA segments in termini, namely 5'-GUUAAA... and ...ACUUAC-3'. The comparison of the IBAV L3 and S7 sequences with those of other two EHDV-2 isolates revealed the higher homologies of 93% and 92% against EHDV-2 Australia isolate (EHDV-2AUS) and lower homologies of 80% and 81% against EHDV-2 North America isolate, respectively. The phylogenetic analysis based on L3 and S7 genes also indicated close relationships between IBAV and EHDV-2AUS. KEY WORDS: dsRNA gene, lbaraki virus, inner capsid, VP3, VP7.  相似文献   

An investigation of heavy metal exposure and risks to wildlife in the Kafue flats of Zambia     

Syakalima O  Choongo K  Nakazato Y  Onuma M  Sugimoto C  Tsubota T  Fukushi H  Yoshida M  Itagaki T  Yasuda J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):315-318

Exposure and ecological risks to heavy metals (copper, zinc, manganese, iron) at Lochnivar and Blue Lagoon National Parks in wildlife dependent on the Kafue river contaminated with mining waste was evaluated. Samples included water, fish, grasses and Kafue Lechwe (Kobus leche kafuensis) liver. At both parks copper ranged from 0.03-0.04 mg/l; 3.0-6.0 mg/kg; 11.0-44.0 mg/kg; trace -199.0 mg/kg; while zinc was 0.01 mg/l; 32.0-82.0 mg/kg; 15.0-21.0 mg/kg; and 52.0-138.0 mg/kg; in water, fish, grasses and lechwe, respectively. Manganese ranges were 0.15-0.16 mg/l; 7.0-18.0 mg/kg; 51.0-145.0 mg/kg; and 40.0-53.0 mg/kg while iron ranges were 0.13-0.14 mg/l; 26.0-134.0 mg/kg; 1766.0-1797.0 mg/kg; and 131.0-856.0 mg/kg; in water, fish, grasses and lechwe, respectively. Levels in all samples except water were high indicating potential for adverse effects.  相似文献   

Antimicrobial Activity of Culture Filtrate of Bacillus amyloliquefaciens RC-2 Isolated from Mulberry Leaves   总被引：1，自引：0，他引：1  

Yoshida S  Hiradate S  Tsukamoto T  Hatakeda K  Shirata A 《Phytopathology》2001,91(2):181-187

ABSTRACT A potential antagonist, Bacillus amyloliquefaciens strain RC-2, against Colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. Application of culture filtrate of RC-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. Development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fungal inoculation, and it was not inhibited by application after inoculation. These results suggest that the antifungal compounds in the filtrate exhibit a preventive effect on the disease. Peptone significantly increased production of the antifungal compounds. The culture filtrate of RC-2 also inhibited the growth of several other phytopathogenic fungi and bacteria, such as Rosellinia necatrix, Pyricularia oryzae, Agrobacterium tumefaciens, and Xanthomonas campestris pv. campestris, in vitro. From the culture filtrate of RC-2, seven kinds of antifungal compounds were isolated by high performance liquid chromatography analysis, and one of the compounds was determined as iturin A2, a cyclic peptide, by nuclear magnetic resonance and fast atom bombardment mass analysis.  相似文献   

Invasion and colonization of mature apple fruit by Erwinia amylovora tagged with bioluminescence genes     

Koji Azegami  Takanori Tsukamoto  Takayuki Matsuura  Tatsuji Ohara  Yasuhiro Inoue  Akifumi Mizuno  Kouji Yoshida  Hideo Bessho  Shigeru Kimura  Masao Goto 《Journal of General Plant Pathology》2005,71(1):98-98

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Studies on red light-induced resistance of broad bean to Botrytis cinerea: I. Possible production of suppressor and elicitor by germinating spores of pathogen     

Nurun Nahar Khanam  Junichi Kihara  Yuichi Honda  Toshihide Tsukamoto  Sakae Arase 《Journal of General Plant Pathology》2005,71(4):285-288

When detached broad bean leaves were preinoculated with virulent strain B304 of Botrytis cinerea 24 h before a challenge inoculation with strain B304, lesion formation by B304 was significantly inhibited in red light but not in the dark. In leaves that were preinoculated with avirulent strain 021 and then challenged by B304, however, lesion formation was not inhibited even under red light. Such differences in lesion formation after the challenge inoculation with an avirulent strain were also observed with lesions caused by Alternaria alternata, a nonpathogen of broad bean and by avirulent strain 021R in the presence of germination fluid from spores of strains B304 and 021R. These results suggest the possibility that virulent B. cinerea produced a suppressor involved in induced susceptibility and an elicitor involved in resistance induced by red light during spore germination.  相似文献   

Restriction fragment length polymorphism as a genetic marker of the c-myc oncogene in rats     

T Kunieda  M Matsui  N Nomura  R Ishizaki  M C Yoshida  T Imamichi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(6):1295-1298

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In vitro fertilization of pig oocytes matured in vivo     

M Yoshida 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(4):711-718

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Pharmacological studies on doping drugs for race horses. IV. Chlorpromazine and phenobarbital (author's transl)]     

S Fujii  S Inada  S Yoshida  C Kusanagi  K Mima 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1975,37(2):133-139

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Generation of canine dendritic cells from peripheral blood mononuclear cells     

Yoshida H  Momoi Y  Taga N  Ide K  Yamazoe K  Iwasaki T  Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):663-669

Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.  相似文献   

Three dogs under 2 years of age with heartworm caval syndrome     

Hidaka Y  Hagio M  Murakami T  Okano S  Natsuhori K  Narita N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1147-1149

Heartworm caval syndrome (CS) occurred in three dogs under 2 years of age. A 23-month-old dog was recovered by surgical and medical treatments, but the other 2 dogs (15 and 21 months old) died. Necropsy demonstrated 12 heartworms in the 15-month-old dog and 8 worms in the 21-month-old dog. Histopathologically, pulmonary arterial embolism caused by dead worms and thrombi were observed in these cases. The findings suggested that CS could develop regardless of canine age and worm burden if pulmonary arterial embolism related to worm death or thrombus formation were induced.  相似文献