Total and differential leukocyte counts of traumatic gastritis classified under three types in dairy cows     

Y Yoshida 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(6):1215-1219

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Antigenic analysis of avian Chlamydia psittaci using monoclonal antibodies to the major outer membrane protein.   总被引：1，自引：0，他引：1  

A Kikuta  N Furukawa  T Yoshida  H Fukushi  T Yamaguchi  K Hirai 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(3):385-389

Monoclonal antibodies to the major outer membrane protein (MOMP) of Chlamydia psittaci derived from a parrot were established for antigenic analysis of avian C. psittaci. With 17 monoclonal antibodies to MOMP, 17 reactivity patterns were identified on 112 strains of C. psittaci, C. pneumoniae and C. trachomatis, which were isolated from birds, mammals and humans in Japan, U.S.A., Canada and Taiwan, from 1938 to 1987. Immunological reactivity of budgerigar-derived strains to the monoclonal antibodies was different from that of pigeon-derived strains. Imported bird-derived strains were distinguishable from domestic bird-derived strains by the reactivity to the monoclonal antibodies. A close relationship between the subtypes and geographic origins was indicated on budgerigar-derived strains. On the contrary, various reactivity patterns were shown in pigeon-derived strains isolated in a narrow area. The monoclonal antibodies established in the present work may be useful probes for ecological study of avian C. psittaci.  相似文献   

Incidence of silent ovulation in dairy cows during post partum period     

Isobe N  Yoshimura T  Yoshida C  Nakao T 《DTW. Deutsche tier?rztliche Wochenschrift》2004,111(1):35-38

The present study was undertaken to show the incidence of silent ovulation in high producing dairy cows, by monitoring ovarian cyclicity based on practical milk progesterone assay which was established in this study. The direct milk progesterone enzyme linked immunosorbent assay (ELISA) was developed using anti-progesterone-3(E)-carboxymethyloxime-BSA antibody for the antibody and horse radish peroxidase labeled progesterone for tracer. High sensitivity (26 pg/mL, 0.65 pg/well), high recovery rates (83-97%), high reproducibility (CV of intra-assay 4.8-11.5%; CV of inter-assay 14.3-19.1%) and high correlation between milk progesterone concentrations measured by the direct ELISA and the values obtained by the ELISA after extraction proved the reliability of the assay. In second experiment the incidence of silent ovulation was investigated based on the milk progesterone concentrations in 32 dairy cows within 70 days post partum. The incidences of silent ovulation at the first, second, third and fourth ovulation post partum were 83, 46, 13 and 0%, respectively. Most commonly observed patterns of sequential occurrence of silent ovulation in cows ovulating 2, 3 or 4 times within 70 days post partum were silent-estrus (50%), silent-silent-estrus (60%) and silent-silent-estrus-estrus (67%), respectively. These results suggest that the present ELISA is a reliable and practically applicable method for determination of progesterone in milk and that high producing dairy cows show a high incidence of silent ovulation at the first post partum ovulation as well as the second ovulation, which then decreased with the increased frequency of ovulation.  相似文献   

A novel repeated sequence located on the bovine Y chromosome: its application to rapid and precise embryo sexing by PCR     

Kageyama S  Yoshida I  Kawakura K  Chikuni K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):509-514

A novel repeated sequence specific to male cattle was identified and named S4. S4 is a highly repetitive sequence and is a 1.5 kb repeating unit that contains various internal repeated sequences. FISH analysis showed that S4 is localized on the whole long arm and the proximal region of the short arm of the Y chromosome. We found that a PCR primer set for S4 amplified a male-specific 178 bp product in addition to a 145 bp product common to both male and female cells. Although the origin of the 145 bp product is unknown, it acts as a positive internal control in practical embryo sexing. Due to the high copy number of S4, PCR required only 0.5 pg purified DNA for accurate amplification. This made it possible to reduce the amount of biopsy sample required for embryo sexing and thus result in less damage to embryos manipulated. These studies indicate that embryo sexing based on the S4 sequence is accurate and sensitive.  相似文献   

Chicken peripheral blood CD3+CD4-CD8- cells are regulated by endocrine and nerve systems     

Kushima K  Yoshida K  Fujita M  Shigeta A  Horiuchi H  Matsuda H  Furusawa S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):143-148

The existence of CD3(+)CD4(-)CD8(-) T cells in thymus and spleen has already been known. However, because of the presence of large amounts of thrombocytes in peripheral blood (PB), the proportion of CD3(+)CD4(-)CD8(-) T cells in PB has yet to be investigated. Therefore, the proportion of peripheral T cell-subsets was investigated in 6-week-old chickens. The percentage of CD3(+) cells, CD4(+) cells, CD8 alpha(+) cells, CD8 beta(+), and CD3(+)CD4(-)CD8(-) cells was 76%, 41%, 14%, 5%, and 15%, respectively. The proportion of CD3(+)CD4(-)CD8(-) cells in PB increased during egg-laying periods and in chickens treated with an analog of estrogen, while it decreased with age and in response to restraint stress. All of the CD3(+)CD4(-)CD8(-) cells expressed TCR1, and did not have NK activity. CD3(+)CD4(-)CD8(-) cells represent about 60% of peripheral TCR1(+) cells. These findings indicate that the proportion of CD3(+)CD4(-)CD8(-) cells is regulated by the endocrine and nerve systems.  相似文献   

Establishment of a large-scale purification procedure for purified recombinant bovine interferon-tau produced by a silkworm-baculovirus gene expression system     

Nagaya H  Kanaya T  Kaki H  Tobita Y  Takahashi M  Takahashi H  Yokomizo Y  Inumaru S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1395-1401

We developed a procedure for the large-scale purification of bovine interferon-tau (boIFN-tau) by means of a silkworm-baculovirus gene expression system. Recombinant boIFN-tau (rboIFN-tau) was efficiently produced in the silkworm infected with boIFN-tau cDNA recombinant baculovirus and accumulated in the haemolymph. To establish a purification method suitable for mass production, we tried three crude purification methods, namely, an acidification and neutralization treatment (ANT), silica gel column chromatography (SGCC), and Blue sepharose column chromatography (BSCC) with a combination of Q-sepharose (QSC) and chelating sepharose column chromatographies (CSCC). As a result, the acidification and neutralization treatment was found to be the most efficient and cost effective. With this combination, we obtained 91% pure products. To confirm the applicability of the procedure for mass production, we inoculated 100 silkworms with the recombinant virus, and recovered about 4.55 mg (1.26 x 10(8)U/mg) of 91% pure rboIFN-tau by means of a combination of the ANT, followed by QSC and CSCC.  相似文献   

Maternal exposure to low doses of bisphenol a has no effects on development of female reproductive tract and uterine carcinogenesis in Donryu rats     

Yoshida M  Shimomoto T  Katashima S  Watanabe G  Taya K  Maekawa A 《The Journal of reproduction and development》2004,50(3):349-360

Effects of maternal exposure to low doses of bisphenol A (BPA), including those comparable with human exposure levels, on growth and development of the female reproductive system and uterine carcinogenesis in Donryu rats were investigated. Dams were administered BPA (0, 0.006 and 6 mg/kg/day) daily by gavage from gestation day 2 up to the day before weaning (postnatal day 21 at offspring). The serum levels of BPA were significantly elevated in the dams receiving 6 mg/kg/day, however, BPA levels in the milk of dams, and those in the serum and liver of offspring were similar between control and treated groups. The treatment did not exert any influences on uterine development including weight, gland genesis and estrogen receptor alpha expression, vaginal opening and gonadotropin secretion in the female offspring up to puberty. After maturation, no effects were evident with regard to estrous cyclicity in female offspring treated with BPA. In addition, the treatment had no effects on age-related morphological changes of the reproductive and endocrine organs and uterine carcinogenesis until 15 months of age. The results demonstrate that maternal exposure to BPA at levels comparable to human exposure did not have any effects on the female reproductive system of offspring in rats. In addition, BPA was also found in the serum, milk and liver of control dams and pups, and low levels of BPA were detected in drinking water and pellet diet. The present study showed that the experimental animals were also exposed to environmental BPA in the animal room.  相似文献   

Studies on the control of Fusarium head blight of barley and wheat and mycotoxin levels in grains based on time of infection and toxin accumulation     

Megumi Yoshida 《Journal of General Plant Pathology》2012,78(6):425-426

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Geochemistry. Tracking the Fukushima radionuclides     

Yoshida N  Kanda J 《Science (New York, N.Y.)》2012,336(6085):1115-1116

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Genetic analyses of agronomic traits in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.)     

Chunhua Li  Kiwa Kobayashi  Yasuko Yoshida  Ryo Ohsawa 《Breeding Science》2012,62(4):303-309

The consumption of products made from Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) has increased in recent years in Japan. Increased consumer demand has led to recognition of the need for early varieties of this crop with high and stable yields. In order to accomplish this, more information is needed on the genetic mechanisms affecting earliness and yield. We conducted genetic analysis of 3 agronomic traits (days to flowering, plant height and total seed weight per plant) to segregate F₂ and F₃ populations derived from a cross between Tartary buckwheat cultivars ‘Hokuriku No. 4’ and ‘Ishisoba’. Broad-sense heritability estimates for days to flowering, plant height and total seed weight were 0.70, 0.62 and 0.75, respectively, in F₃ population. Narrow-sense heritability for total seed weight (0.51) was highest, followed by heritability for days to flowering (0.37), with heritability for plant height (0.26) lowest. Later flowering was associated with increased plant height and higher yields. From the F₄ generation, we identified twelve candidate plants with earlier maturity and reduced plant height compared to ‘Hokuriku No. 4’, but almost the same total seed weight. These results suggest that hybridization breeding using the single seed descent (SSD) method is an effective approach for improving agronomic characteristics of Tartary buckwheat.  相似文献