副猪嗜血杆菌单克隆抗体的制备及其B细胞抗原表位的鉴定     

杨玉菊  郑楠  符芳  柴政  姜福成  王香玲  张雪云  王卓  李曦 《中国预防兽医学报》2014,(5):400-403

为筛选具有临床诊断价值的副猪嗜血杆菌(H.parasuis)单克隆抗体(MAb),本研究采用H.parasuis 5型强毒株(HPS-5)免疫BALB/c小鼠,利用常规细胞融合技术制备了一株稳定分泌MAb的杂交瘤细胞株。Western blot结果表明该MAb能够特异性识别所有H.parasuis标准菌株;免疫共沉淀技术与蛋白质谱分析显示该MAb所识别的蛋白为ATP结合盒转运蛋白(ABCT)家族的寡肽透过酶(OppA);利用MAb对噬菌体12肽库进行淘选,8个阳性噬菌体克隆展示有"KTPAE-R"保守序列,对应于H.parasuis SH29755株和SH0165株的469位~475位的氨基酸残基。对OppA氨基酸序列比对结果表明H.parasuis与其它10种猪常见细菌病病原序列一致性为9.1%~74.5%,本研究结果为进一步研究H.parasuis感染的病原学诊断建立了良好的基础。  相似文献   

E. Melanie DuPuis: Nature’s perfect food: how milk became America’s drink     

Evan Perrault 《Agriculture and Human Values》2011,28(4):583-584

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Books received     

Carol J. Pierce Colfer 《Agriculture and Human Values》2011,28(4):589-590

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Performance evaluation of forest co-management: a case study of Chunati Wildlife Sanctuary,Bangladesh     

Umma Tamima 《林业研究》2016,27(4):853-861

The objective was to evaluate the performance of the co-management of Nishorgo Support Project at Chunati Wildlife Sanctuary in Bangladesh. I adopted the Focus Groups Discussion method for opinion survey and applied the SWOTAHP technique for data analysis. Local people did not participate in the decision-making process of forest management and they perceived co-management as a threat to their livelihoods. Moreover, mistrust and misunderstanding among different stakeholders, political and ethical conflicts, lack of property rights of tribal communities, and lack of accountability and transparency were identified as the drawbacks of co-management.  相似文献   

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys     

WANG Jing-jing  ZHANG Xue-mei  ZHAO Hong-ling  DONG Cheng-hong  PU Jing  LIAO Yun  WANG Li-chun  NA Rui-xiong  LIU Long-ding  ZHANG Ying  YANG Li-xian  WANG Qing-ling  SHEN Dong  WANG Xi  LI Qi-han 《园艺学报》2011,27(7):1249-1256

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.  相似文献   

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig     

QIN Zhao-xian  NONG Su-qun  GUAN Zhi-hui  LIANG Dan  QIN Qian  LAN Xi  PAN Tian-biao  XIE Bing-kun 《中国畜牧兽医》2016,43(9):2223-2229

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.  相似文献   

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究     

李春艳  王曦  周胜花  李希  钟华  董宽虎 《草地学报》2020,28(6):1784-1790

本文以白羊草（Bothriochloa ischaemum）为材料，利用其目前已有的转录组序列，通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段，对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子，它们具有高度保守的[W]-X（19）-[W]-X（19）-[W]-X（n）-[W]-X（18）-[W]结构，主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析，本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。  相似文献   

肾上腺皮质肿瘤良恶性鉴别方法探究     

熊蔚蔚  郭菁  张成义  陈曦 《吉林林学院学报》2011,(6):669-673

肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）是一种常见的肿瘤,存在于人体的内分泌系统中,并且是引发肾上腺皮质癌（Adrcnocortical Carcinoma,ACC）进而导致死亡的主要原因.经典的＂Weiss系统＂在诊断明确性上存在种种无法解决的问题,但随着科技水平的发展,人们开始探索新的途径来鉴别ACT的良恶性,于是开始有了影像学和分子生物学技术等特异性的检查方法,从而进一步增强了诊断的明确性.现通过对比的方式,探究肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）影像学检查和分子生物学技术检查的结果,从而能更准确地鉴别其良恶性.  相似文献   

Jody Emel and Harvey Neo: Political ecologies of meat     

Heide K. Bruckner 《Agriculture and Human Values》2016,33(3):739-740

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Brian K. Obach: Organic struggle: the movement for sustainable agriculture in the United States     

Kelly Yearick 《Agriculture and Human Values》2017,34(2):505-506

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