Molecularly Imprinted Polymer (MIP) Applications in Natural Product Studies Based on Medicinal Plant and Secondary Metabolite Analysis     

Zahra Karimi Baker  Soroush Sardari 《Iranian Biomedical Journal》2021,25(2):68

Characterization and extraction of plant secondary metabolites are important in agriculture, pharmaceutical, and food industry. In this regard, the applied analytical methods are mostly costly and time-consuming; therefore, choosing a suitable approach is essential for optimum results and economic suitability. One of the recently considered methods used to characterize new types of materials is MIPs. Among the various applications of MIPs is the identification and separation of various plant-derived compounds, such as secondary metabolites, chemical residues, and pesticides. The present review describes the application of MIPs as a tool in medicinal plant material analysis, focusing on plant secondary metabolism. Key Words: Molecularly imprinted polymers, Plants medicinal, Secondary metabolism  相似文献   

Latency-Associated Transcript-Derived MicroRNAs in Herpes Simplex Virus Type 1 Target SMAD3 and SMAD4 in TGF-β/Smad Signaling Pathway     

Zahra Shojaei Jeshvaghani  Ehsan Arefian  Sara Asgharpour  Masoud Soleimani 《Iranian Biomedical Journal》2021,25(3):169

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Clinical and Genetic Characteristics of Splicing Variant in CYP27A1 in an Iranian Family with Cerebrotendinous xanthomatosis     

Zahra Rashvand  Kimia Kahrizi  Hossein Najmabadi  Reza Najafipour  Mir Davood Omrani 《Iranian Biomedical Journal》2021,25(2):132

Background:CTX is a rare congenital lipid-storage disorder, leading to a progressive multisystem disease. CTX with autosomal recessive inheritance is caused by a defect in the CYP27A1 gene. Chronic diarrhea, tendon xanthomas, neurologic impairment, and bilateral cataracts are common symptoms of the disease. Methods:Three affected siblings with an initial diagnosis of non-syndromic intellectual disability were recruited for further molecular investigations. To identify the possible genetic cause(s), WES was performed on the proband. Sanger sequencing was applied to confirm the final variant. The clinical and molecular genetic features of the three siblings from the new CTX family and other patients with the same mutations, as previously reported, were analyzed. The CYP27A1 gene was also studied for the number of pathogenic variants and their location. Results:We found a homozygous splicing mutation, {"type":"entrez-nucleotide","attrs":{"text":"NM_000784","term_id":"1519312912"}}NM_000784: exon6: c.1184+1G>A, in CYP27A1 gene, which was confirmed by Sanger sequencing. Among the detected pathogenic variants, the splice site mutation had the highest prevalence, and the mutations were mostly found in exon 4. Conclusion:This study is the first to report the c.1184+1G>A mutation in Iran. Our findings highlight the other feature of the disease, which is the lack of relationship between phenotype and genotype. Due to nonspecific symptoms and delay in diagnosis, CYP27A1 genetic analysis should be the definitive method for CTX diagnosis. Key Words: Cerebrotendinous xanthomatosis, CYP27A1, Intellectual disability, Iran, Whole exome sequencing  相似文献   

Comparative study on hormones and biochemistry indices in plasma,ovarian fluid and oocytes of LHRHa2‐induced female Stellate sturgeon (Acipenser stellatus)     

Zahra Roosta  Sareh Ghiasi  Bahram Falahatkar 《Aquaculture Research》2019,50(1):139-145

Reproduction efficacy of sturgeon as donors of oocytes is regulated by hormones effects. It can influence the egg quality or larvae functions as rearing mediators for successful production. This study is conducted to evaluate the concentration of sex steroids, cortisol and thyroid hormones and distinguish the relationship of maternal hormones' correlation in Stellate sturgeon. The study followed the hormones fluctuations that could impress some egg quality information such as fertilization rate. Female breeders were injected with LHRH_a2 for controlled propagation. Samples from blood, ovarian fluid and oocytes were obtained for hormonal and biochemical analysis. Our results showed that the concentrations of estradiol 17‐β and testosterone were significantly higher and lower in oocyte and the plasma, respectively. The level of plasma cortisol was significantly higher than in ovarian fluid and oocytes. The results showed elevation of triiodothyronine and thyroxine concentrations in oocytes, which were significantly different with plasma and ovarian fluid. Thyroxine concentration of plasma and ovarian fluid revealed a significant negative correlation. The mentioned parameters showed negative correlation with fertilization rate. The biochemical parameters (calcium, glucose, cholesterol and triglyceride) revealed significant differences in blood, ovarian fluid and oocytes. These results showed direct relations of fertilization rate with hormones level. Therefore, fertilization rate as egg quality index could be recommended as a relevant indicator of maternal transferring of hormones from blood and ovarian fluid to oocyte.  相似文献   

Molecular characterization of a pigeon paramyxovirus type 1 virus isolated from Eurasian collared doves in Iran, 2017     

Reza Esmaeelzadeh-Dizaji  Aidin Molouki  Hossein Hosseini  Mohammad Hossein Fallah-Mehrabadi  Zahra Ziafati-Kafi  Azin Takalou  Nava Eram  Niloufar Kumar  Alireza Ashuri  Naser Sadri  Arash Ghalyanchi-Langeroudi 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(3)

In September 2017, an outbreak with high mortality, which showed the typical signs of ND, occurred among a flock of more than 2000 Eurasian collared doves in Konarak, southeast of Iran. A confirmed pigeon paramyxovirus type 1 strain was isolated from the brain tissues of the dead doves. The isolate, which was called Pigeon/Iran/Konarak/Barin/2017, was classified as a highly velogenic NDV. Complete genome sequencing and phylogenetic analysis showed that the isolate belonged to subgenotype XXI.2, which has never been reported from Iran before. The isolate had the highest homology (96.15%) with early 2010s Italian isolates. Further studies will be required to understand the diversity better.  相似文献