Identification and Molecular Characteristics Analysis of Porcine Epidemic Diarrhea Virus Variants     

ZHANG Zhi  CHENG Xiao-na  FAN Ya-ting  DONG Ya-qin  LIU Shuang  WU Fa-xing  SHAO Wei-xing  LI Xiao-cheng 《中国畜牧兽医》2015,42(7):1654-1660

To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains.  相似文献   

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice     

LIU Lin  #  ZHANG Ying xin  #  LI Zhi  LIU Qun en  YU Ning  SUN Bin  YANG Zheng fu  ZHOU Quan  CHENG Shi hua  CAO Li yong 《中国水稻科学》2014,28(5):465

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Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells     

LI Fu-hai  LI Zong-zhuang  JIANG Zhi  YI Wei  ZHANG Chen-yun 《园艺学报》2015,31(6):1130-1136

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.  相似文献   

农业科研单位编制外用工的管理策略     

陈洁  张智  蚁秀清 《热带作物学报》2016,(3):72-74

编制外用工是农业科研单位普遍存在的用工形式。本文阐述了农业科研单位编制外用工的特点，分析了编制外用工存在问题及原因，提出从规范用工管理方式、强化考核管理、拓宽发展空间等方面提高编制外人员的工作积极性，提升编制外用工人力资源管理的效能，促进单位发展。  相似文献   

基于GRA-TOPSIS耦合的草莓品质综合评价     

张智  党思荣  李曼宁  朱姝仪  李大勇  杨晓庆 《东北农业大学学报》2021,52(10):47-56

研究不同水肥滴灌条件对草莓品质的综合影响,为草莓高品质生产提供水肥管理依据.以灌水量(X1)和施肥量(X2)为试验因子,依据蒸腾量(ETc)设置4个灌水水平(W1:100％ETc、W2:85％ETc、W3:70％ETc、W4:55％ETc);依据目标产量(F0)设置3个施肥水平(F1:120％F0、F2:100％F0、F3:80％F0),完全组合共12个处理.测定草莓可溶性总糖、可溶性蛋白、维生素C、可溶性固形物、糖酸比、游离氨基酸、还原性糖、硝酸盐8个品质指标,解析水肥耦合对各品质指标的调控效应.各品质指标对水肥因素响应不一致,基于博弈论组合赋权中,单一指标权重最大是可溶性蛋白为0.1689,维生素C次之为0.1551,硝酸盐最小为0.0918;基于GRA-TOPSIS建立草莓品质综合评价,解析草莓综合品质对水肥因素响应模型,可知,水肥交互作用显著,且施肥效应强于灌水.当灌水量控制在2 003～2566m3.hm-2,施肥量控制在1 878～2 195 kg.hm-2时,草莓综合品质最佳.  相似文献   

吉林省蜜蜂疫病危害现状及发展对策     

王志  陈东海  常帅  刘楠楠  柏建民  杨春红  牛庆生 《蜜蜂杂志》2014,34(12):3-6

调查分析了吉林省主要蜜蜂饲养区蜜蜂疫病危害的现状,发现蜜蜂白垩病(56%)、蜂螨(40%)及爬蜂病(20%)为吉林省3大主要蜜蜂病害,同时通过实验室检测病蜂样本发现,蜜蜂微孢子虫的隐性感染情况较为严重,32%的蜂场存在不同程度的感染,应引起重视。蜜蜂敌害中胡蜂危害(42%)较为严重,个别蜂场兼受地胆、蚂蚁及蟾蜍的侵害。蜂病的确诊是目前饲养者面临的第一难题,建议尽快建立蜜蜂疫病防治技术体系,宣传蜜蜂健康养殖技术,加强流行病学及相关基础研究,加大抗病蜂种的培育力度,加快无公害对症蜂药的研究进展,给饲养者养成防患于未然、饲养强群等优良习惯,为蜜蜂产业的健康发展奠定良好的基础。  相似文献   

网室内棉花不育系蜜蜂授粉试验研究     

夏芝  杨慧勇  吴翠翠  侯保国 《中国棉花》2019,46(2):16-18

本研究在网室内利用蜜蜂为6个棉花不育系材料授粉，以人工授粉作为对照，研究蜜蜂授粉和人工授粉在农艺性状等指标上的差异。结果表明，蜜蜂授粉和人工授粉的单株结铃数和空果枝数有极显著差异；不同不育系的单株结铃数差异极显著，果枝数有显著差异。晋56-1A、晋56-3A、晋56-6A的单株结铃数在蜜蜂授粉和人工授粉间有显著性差异，晋56-2A和晋56-5A的单株结铃数在不同授粉方式下有极显著差异；晋56-4A和晋56-6A的空果枝数有极显著差异。室内考种表明，蜜蜂授粉的衣分范围为26.3%～39.6%，人工授粉的衣分为29.4%～35.3%，人工授粉衣分范围相对较小。人工授粉的单铃籽粒数、制种产量均比蜜蜂授粉大，铃重、籽指、发芽率相差不大。蜜蜂授粉制种产量达人工授粉的80%左右；而制种成本可节约50%以上。因此，选择强群势蜂群、适时有效的虫害管理等措施，可提高棉花不育系制种效率，对棉花“三系”的发展有重要的推动作用。  相似文献   

一次降雪的积雪密实化过程研究   总被引：1，自引：0，他引：1  

刘宝河  左合君  董智  王嫣娇  杨阳  闫敏  李钢铁 《干旱区资源与环境》2017,31(1):178-184

通过对呼和浩特市冬季一次降雪后积雪厚度和积雪密度两个指标的持续测量,研究了观测期18d内的积雪密实化过程,分时段和观测时间点对两个指标的变化过程进行对比分析.结果表明:比较发现积雪厚度具有更好的阶段变化特征,因此依据积雪厚度划分法,将积雪密实化过程分为剧烈变化期(前24h)、快速变化期(24h-48h)和稳步发展期(48h之后)三个阶段.其中剧烈变化期和快速变化期分别完成整个积雪密实化过程的52.63％和12.17％,说明积雪在前期较短时间内快速密实;短期内不同观测时间点的积雪密度由于受气温的主要影响出现小幅波动,14:00测得的积雪密度值最高,8:00和20:00差别不大.相关研究结果可为季节性积雪区积雪资源的估算与利用提供理论依据.  相似文献   

饲粮铜源及水平对育成期雄性水貂生长性能、营养物质表观消化率及氮、铜代谢的影响   总被引：1，自引：0，他引：1  

吴学壮  高秀华  杨福合  张铁涛  崔虎  刘志  郭俊刚  邢秀梅 《动物营养学报》2015,(3):795-803

本试验旨在研究饲粮铜源及水平对育成期雄性水貂生长性能、营养物质表观消化率及氮、铜代谢的影响。采用3×3+1双因素试验设计,设3种铜源(硫酸铜、碱式氯化铜、蛋氨酸铜)和3个铜添加水平(10、25、40 mg/kg),1个对照组。选择60日龄健康、体重接近的美国短毛黑雄性水貂120只,随机分成10组,每组12个重复,每个重复1只貂。预试期7 d,正试期45 d。结果表明:1)水貂的平均日采食量随饲粮铜添加水平的增加呈极显著线性增加(P0.01),并且平均日增重随饲粮铜添加水平的增加也呈线性增加(P=0.053)。2)水貂的粗脂肪表观消化率随饲粮铜添加水平的增加呈极显著线性增加(P0.01),并且粗蛋白质表观消化率随饲粮铜添加水平的增加也呈显著线性增加(P0.05)。3)水貂铜摄入量、粪铜排泄量、尿铜排泄量以及铜沉积量均随饲粮铜添加水平的增加呈极显著线性增加(P0.01),然而铜的表观吸收率却随饲粮铜添加水平的增加呈极显著线性降低(P0.01)。水貂对碱式氯化铜和蛋氨酸铜的表观吸收率极显著高于对硫酸铜的表观吸收率(P0.01)。水貂氮沉积量随饲粮铜添加水平的增加而呈显著线性增加(P0.05)。由此可见,基础饲粮中铜含量为8.05 mg/kg时,饲粮添加40 mg/kg的铜能改善育成期雄性水貂生长性能,提高水貂粗脂肪表观消化率;育成期雄性水貂对碱式氯化铜和蛋氨酸铜的表观吸收率高于对硫酸铜的表观吸收率。  相似文献   

Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella)     

Wei Hu  Zhi Luo  Kang-Sen Mai  Cai-Xia Liu  Jia-Lang Zheng 《Fish physiology and biochemistry》2015,41(6):1393-1401

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