Effect of chlorogenic acid on intestinal inflammation,antioxidant status,and microbial community of young hens challenged with acute heat stress     

Fang Chen  Hao Zhang  Na Zhao  Xuehai Yang  Encun Du  Shaowen Huang  Wanzheng Guo  Wei Zhang  Jintao Wei 《Animal Science Journal》2021,92(1):e13619

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA₃₀₀ group and HS + CGA₆₀₀ group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA₃₀₀, and HS + CGA₆₀₀ group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.  相似文献   

民族种业发展战略构想     

郝恩玉 《种子世界》2001,(Z1)

“民以农为本,农以种为先“,种业是农业的上游产业之一。《种子法》的颁布实施,标志着种子产业已进入市场经济的轨道,多元化结构的种子产业时代已经到来,揭开了民族种业发展的新篇章。在这特定的历史条件下,面对一体化的全球经济,面对“入世“的到来,作为终身执着于种子产仆的有志者们,抓住机遇迎接挑战,勇于竞争,赢得未来,这是我们无法回避的现实。  相似文献   

Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model     

LI Zhong  ZHANG Cheng  CHEN Guo-jun  LIU Xiao-rong 《园艺学报》2004,20(3):311-316

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.  相似文献   

钙在橄榄树器官中的定量研究   总被引：3，自引：0，他引：3  

钟明  甘廉生 《中国南方果树》2004,33(4):21-23

本研究用原子吸收光谱法分析了果园土壤及橄榄树体各器官中钙量和不同组分钙的含量。结果表明：0～30cm土层中钙含量最高为0．65mg／g。橄榄器官中叶的钙含量最高为4．523mg／g，其次为根、果、结果枝。地上部器官钙含量由主干一主枝一结果枝一叶片逐渐增加。在各器官中钙主要以醋酸溶钙和盐酸溶钙的形式存在。  相似文献   

落叶松人工林林分径级结构的研究     

仲秋维  于常玲 《林业科技》1996,21(1):17-19

本文描述了落叶松人工林抚育后径级结构特征。试验表明，抚育后的林分在一定密度条件下，其密度遵从Ｗｅｉｂｕｌｌ分布，并能保持相当长的时间不变；其密度较大，Ｗｅｉｂｕｌｌ参数值在２～３间变动，ｂ值在６～８间变动；稀疏时不能用Ｗｅｉｂｕｌｌ分布模拟。  相似文献   

毛竹残次林改造试验报告     

钟承广 《竹子研究汇刊》1996,15(2):20-27

１９８７～１９９０年，对３５１５ｈｍ２残次毛竹林试验区进行４年２度的砍杂、全垦、全垦结合施肥３种不同的作业方式的对比试验，试验结果均比试验前取得了显著的效益，其平均立竹量由１７１６支／ｈｍ２增加到３０７５支／ｈｍ２，平均胸径由６．６７ｃｍ增加到８．９１ｃｍ，平均度产新竹由３７８０ｋｇ／ｈｍ２增加到１５０９０ｋｇ／ｈｍ２，平均度产竹笋由５７０ｋｇ／ｈｍ２增加到２４３０ｋｇ／ｋｍ２，度产值由７９５元／ｈｍ２增加到３２３５．５元／ｈｍ２，投入产出比为１：７．０８。３种作业方式的比较，以全垦结合施肥为最好。  相似文献   

竹缺爪螨实验种群生命表的研究     

张飞萍  蔡秋锦  钟景辉 《林业科学》2001,37(3):101-105

在25℃温度条件下，用黄金间碧竹（Bambusa vulgaris cv.vittata)叶饲养竹缺爪螨（Aponychus corpuzae Rimando)，应用生存分析理论和多维矩阵模型组建竹缺爪螨种群生命表，计算得种群主要参数为：内禀增长率rm＝0．0811，净增殖率R0＝5．8368，平均世代长度T＝21．7532d，周限增长率λ=1.0845/d；并分析了种群在不同年龄的生命状态，各发育期重叠情况以及稳定的年龄一阶段分布；最后讨论了应用这2种方法组建螨类生命表的优点。  相似文献   

越桔对长白山区酸性土壤的适应性   总被引：15，自引：1，他引：14  

李亚东  郝瑞 《园艺学报》1994,21(2):129-133

在长白山区３种类型的酸性土壤上栽培越桔，研究结果表明，｀北空＇、｀美登＇、｀斯卫克＇在暗棕色森林土上生长结果较好，树体营养关系合理；在草甸沼泽土上３个种生长结果均较差，｀北空＇在水湿地潜育土上生长旺盛，但产量低，综合分析认为，矮丛越桔类型品种对我国长白山区酸性土壤条件的适应性较强，半高丛越桔则适应性差，针对长白山不同类型的土壤，以美登最为适宜暗棕色森林土栽培，斯卫克次之；水湿地潜育土可栽培美登品种  相似文献   

多层螺旋CT与核磁共振血管成像、数字减影对主动脉夹层诊断的比较研究     

李冲云蔡勇  贺红艳刘志 《湛江医学院学报》2004,22(2):126-128

目的：比较多层螺旋CT(multi-slice spiral CT，MSCT)与核磁共振血管成像(MRA)、数字减影(DSA)对主动脉夹层(aortic dissection，AD)的诊断价值。方法：对17例AD患者行MSCT检查，并与MRA、DSA的检查结果进行比较。结果：对17例AD患者用MSCT、MRA、DSA都能作出诊断(按DeBakey分型，Ⅰ型有4例，Ⅱ型有1例，Ⅲ型有12例)，但只有MSCT能明确显示夹层的范围和分支的受累情况，以及剥离内膜瓣、裂口及主动脉的周围情况。而MRA对裂口的检出率只有21．74％，DSA可达87．0％。结论：MSCT对AD的分型以及破裂口的定位诊断更具准确性，对动脉分支显示更清晰。  相似文献   

4株酸奶噬菌体的分离     

徐成勇  吴昊  顾晓琳  何楚莹  陶仲炎  秦玉青  周凌华  应杰  诸葛健  郭本恒 《乳业科学与技术》2004,26(4):147-149

从上海光明乳业股份有限公司车间发酵罐中分离出4株酸奶噬菌体。1号噬菌体头部呈六角形，平均直径为95.1nm，头部剖面平均面积为8184．8nm^2，尾部的平均直径为18．3nm，平均长度为403．4nm；2号噬菌体头部呈六角形，平均直径为85．4nm，头部剖面平均面积为8046．2nm^2，尾部的平均直径为23．3nm，平均长度为549．4nm；3号噬菌体头部呈六角形，平均直径为95．5nm，头部剖面平均面积为9792．3nm^2，尾部的平均直径为19．8nm，平均长度为450．5nm；4号噬菌体头部呈六角形，平均直径为119．3nm．头部剖面平均面积为12586．1nm^2，尾部的平均直径为23．6nm，平均长度为520．5nm。  相似文献