Differential serological testing by simultaneous indirect immunofluorescent antibody test in canine leishmaniosis and ehrlichiosis     

Guillén Llera JL  López García ML  Martín Reinoso E  De Vivar González R 《Veterinary parasitology》2002,109(3-4):185-190

A mixed indirect fluorescence antibody test (IFAT), based on cultured promastigotes Leishmania infantum and formol-inactivated suspension of cells infected with the bacteria Ehrlichia canis, was applied to make a differential diagnosis between canine ehrlichiosis and leishmaniosis. A titre greater than 80 was considered positive for antibodies to E. canis and suggestive of antibodies to L. infantum. Positive sera were titrated subsequently by serial dilutions to confirm antibodies positive to Leishmania and establishing the antibody titre of both pathogens. Fluorescence was absent with negative control sera and background staining was minimal. No serological cross-reactions between positive sera for L. infantum or E. canis were detected. Results obtained by mixed IFAT did not differ when the same serum IFAT standard was compared. The test showed equivalent sensitivity (100%). The specifities were 100% for L. infantum and 98.5% for E. canis. The equivalence in sensitivity was confirmed by calculating the correlation coefficient between IFAT standards and mixed IFAT (r>or=0.99 for both pathogens). The results of our investigations demonstrated that mixed IFAT is a specific means of establishing serological differential diagnosis of canine leishmaniosis and ehrlichiosis.  相似文献   

Risk factors for stillbirths in two swine farms in the south of Brazil     

Lucia T  Corrêa MN  Deschamps JC  Bianchi I  Donin MA  Machado AC  Meincke W  Matheus JE 《Preventive veterinary medicine》2002,53(4):57-292

We evaluated stillbirth risk factors in two commercial swine farms of the Rio Grande do Sul State (south of Brazil). The study was conducted during 1 month in Farm A and during 2 months in Farm B, both during 1999. Data for all farrowings that occurred during the study period were recorded (101 for Farm A and 373 for Farm B), without interference in the farm management. In Farm A, 39% of all litters born during the period of interest had stillborn piglets and the stillborn risk for piglets was 12%. In Farm B, 25% of all litters had stillborn piglets whereas the stillborn risk was 2%. Variables considered as potential risk factors for stillbirths were: parity (1, 2–3, 4+); breed (purebred or crossbred); sow body-condition (normal or fat); use of oxytocin during parturition (yes or no); obstetric intervention through vaginal palpation (yes or no); farrowing duration (<4 or ≥4 h); mummified fetuses (yes or no); total litter size (<12 or ≥12 piglets); and litter birth weight (<11 or ≥11 kg). All stillborn piglets had their classification validated by necropsy. In multivariable logistic-regressions, the cases were the litters having at least one stillborn piglet. In Farm A, litters having at least 12 pigs and in which oxytocin was used during the parturition had 20.8-times-higher odds of stillborn occurrence. In Farm B, litters from sows having parity ≥4 had 2.2-times-higher odds of stillborn occurrence than litters from parity 2 to 3 females, litters having ≥12 pigs had 2.0-times-higher odds of a stillborn piglet than smaller litters and farrowings in which vaginal palpation was performed had 8.0-times-higher odds. Farrowing room management to minimize stillborn risk should target higher-parity females, large litters and optimization of practices of obstetric interventions.  相似文献   

丹那唑联合维生素D3或(和)阿糖胞苷治疗骨髓增生异常综合征21例     

陈少通  赵淑华 《湛江医学院学报》2000,18(3):276-277

骨髓增生异常综合征(MDS)是骨髓造血干细胞增殖和分化异常的一组疾病,10%～40%可发展为急性白血病,治疗棘手,1993～1999年笔者使用丹那唑联合诱导分化剂治疗MDS21例,报道如下.  相似文献   

Rotavirus and concurrent infections with other enteropathogens in neonatal diarrheic dairy calves in Spain     

García A  Ruiz-Santa-Quiteria JA  Orden JA  Cid D  Sanz R  Gómez-Bautista M  de la Fuente R 《Comparative immunology, microbiology and infectious diseases》2000,23(3):175-183

Faeces samples from 218, one to 30 days old, diarrheic dairy calves in 65 dairy herds were screened for the presence of rotavirus and concurrent infections with coronavirus, Cryptosporidium, F5+ Escherichia coli and Salmonella spp. Calves were grouped according to their age as follows: 1-7, 8-14, 15-21 and 22-30 days. Rotavirus infection was detected in 46.9%, 45.6%, 33.8% and 48.3% of the calves in the respective age-groups. No significant differences in the detection rate of rotavirus were found among calves on the different age-groups. Rotavirus was the only enteropathogen detected in 39 of the 93 (41.9%) diarrheic calves positive to this agent. Concurrent infections with other enteropathogen(s) were detected in 31.3%, 33.3%, 20.6% and 3.4% of the rotavirus infected calves in the age-groups 1-7, 8-14, 15-21 and 22-30 d, respectively. A significant age-associated decrease in the detection rate of mixed infections (p < 0.01) was found. The detection rates of the other enteropathogens considered in calves with rotavirus infection were 20.4% for coronavirus, 85.2% for Cryptosporidium, 16.7% for F5+ E. coli and 1.8% for Salmonella.  相似文献   

Disseminated mycoses in a dog by Paecilomyces sp     

García ME  Caballero J  Toni P  Garcia I  Martinez de Merlo E  Rollan E  Gonzalez M  Blanco JL 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(4):243-249

We describe a case of canine mycoses initially diagnosed by clinical signs and enzyme-linked immunosorbent assay anti-fungal test, and later confirmed by the isolation of Paecilomyces sp. during the post-mortem examination. The fungus was isolated from lesions in the kidneys, mitral valve, abdominal aorta and vertebral discs. In this kind of process, it is important to identify the responsible agent early in order to make a study of anti-fungal susceptibility and establish effective treatment.  相似文献   

颗粒饲料育肥不同品种羯羔效果试验     

曹志东 张涛王维中  曹福文陈学通马先峰  朱强孙京奎  刘彦江裴学义 张兵 《牧草与饲料》2007,1(2):51-53

2005年7-9月，开展了小尾寒羊和萨寒F1羔羊育肥效果测定，试验组分小尾寒羊、萨寒F1和对照组（小尾寒羊）共3组，3月龄羯羔每组10只，试验组饲喂颗粒饲料和少量混合草、对照组喂混合草，采用分组舍饲养殖，定量采食。正试期分二个阶段共60d，结果表明：第一阶段育肥期40d，小尾寒羊、萨寒F1、对照组只均日增重分别达到171．1、250．1和25．8g，各组差异达到极显著水平（P〈0．01）；第二阶段育肥期20d内，只均日增重分别达到237．0、296．5和67．1g（P〈0．01），试验组间比较差异不显著；育肥全期60d，只均日增重分别达到193．0、260．3和39．6g，各组差异达到极显著水平（P〈0．01）。小尾寒羊、萨寒F1、对照组在育肥全期内只均利润分别达到15．95、30．57和-26．50元，投入产出比为1：1．22、1：1．35、1：0．40，料重比为2．99：1、2．90：1。  相似文献   

小粒牧草种子播种器及其播种技术研究     

曹志东 张涛王维中  陈学通马先锋王志明  侯振禄史锦平岳民勤  刘安平虎凌云 《牧草与饲料》2007,1(3):38-39

播种器由种子仓、导粒管、开沟器3部分组成，经试验示范，结果表明：岷山红三叶适宜播种孔径3mm，手动压力使种子仓内缩1．5cm，播种量控制在4．14—4．66kg／hm^2，与实际播种量4．5kg／hm^2相比变幅92．0％～103．6％；岷山猫尾草适宜播种孔径2．5mm，手动压力使种子仓内缩1cm，播量控制在3．58～4．02kg／hm^2，与实际播种量3．75kg／hm^2相比变幅95．5％-107．2％；结合导粒管、开沟器就能使种子均匀地流入播种行中。本播种器播种技术适宜于各种地形条件，在岷县9个乡镇示范推广380hm^2取得成功。  相似文献   

铜的来源和水平对生长猪生长性能禾粪铜排出量的影响     

郑春田蒋宗勇  林映才邹书通 余德谦 《中国畜牧杂志》2007,43(13):24-27

选用平均体重29．6kg的三元杂（杜×大×长）生长猪144头，按随机区组设计分为6个处理。饲粮中分别添加0、100、200mg／kg硫酸铜、200mg／kg碱式氯化铜、100mg／kg小肽铜、100mg／kg复合氨基酸螯合铜（以铜含量计）。结果表明：100mg／kg小肽铜和100mg／kg复合氨基酸螯合铜均能有效提高生长猪日增重和日采食量，其促生长效果与200mg／kg硫酸铜无显著（P〉0．05）差异，而粪中铜含量分别下降了36．8％和48．4％（P〈0．05）。  相似文献   

猪肉品质营养调控的研究进展   总被引：4，自引：0，他引：4  

蒋宗勇  邹书通  林映才  郑春田 《养猪》2006,(2):49-54

此文从能量、脂肪、蛋白质与氨基酸、维生素、矿物元素及其它一些添加剂等几个方面对猪肉品质营养调控的研究进展进行综述,并提出今后值得关注的几个研究方向。  相似文献   

金属离子对霍山石斛类原球茎增殖及植株再生的影响   总被引：5，自引：1，他引：5  

查学强  罗建平  石玮  姜绍通 《园艺学报》2006,33(1):179-181

 采用单因素浓度试验和正交试验研究了Mg²⁺ 、Ca²⁺ 、Fe²⁺ 、Mn²⁺和Zn²⁺离子对液体培养霍山石斛类原球茎增殖的影响。结果表明, 5种金属离子对类原球茎增殖影响的主次顺序为Fe²⁺ >Mn²⁺ >Mg²⁺ >Ca²⁺ > Zn^{2 +} , 促进类原球茎增殖的优化组合为Mg²⁺ 1.5 mmol·L ^-1、Ca²⁺ 4.5 mmol·L ^-1、Fe²⁺ 0.1 mmol·L ^-1、Mn²⁺ 0.5 mmol·L ^-1、Zn²⁺ 0.06 mmol·L ^-1。经优化的液体培养基增殖的类原球茎能保持植株再生能力, 平均每克类原球茎增殖后可再生植株1 985.2株, 是相同时间内对照类原球茎再生植株数的4.2倍。  相似文献