排序方式： 共有52条查询结果，搜索用时 15 毫秒
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【目的】研究不同培养基及不同土样处理方法对植物根际放线菌分离效果的影响,并从中挖掘具有开发潜力的高活性根皮苷降解放线菌株,为苹果连作障碍机制研究及微生物修复提供理论和实践指导。【方法】从吉林、河北、山东、陕西、甘肃、河南、湖北、四川、重庆、上海、江西、安徽、浙江等13个省（市）采集植物根际土样40份,自然风干处理后分别经10 mmol/L的MOPS溶液及CaCO3预处理,用涂布平板法在7种不同的培养基中进行放线菌的分离培养。将分离获得的菌株点种于根皮苷筛选培养基进行活性检测。【结果】在7种培养基B、MSC、MG、MT、Chi、HSG、HV中分别分离获得100、78、108、64、79、113和88株放线菌。土样用MOPS溶液处理后所分离得到的放线菌总数明显高于用CaCO3预处理所得。分离所得630株菌株中有29株对根皮苷有降解活性。对其中41株高活性菌株及形态特殊菌株进行16S rRNA基因测序分析,发现分属于14个不同的放线菌属。【结论】7种分离培养基中HSG培养基的分离效果最好,MG培养基次之,MT培养基最差。土样处理中用MOPS溶液处理的方法优于用CaCO3预处理。根际放线菌株中有一定数量具有降解根皮苷能力的菌株。根际放线菌资源具有丰富的生物多样性。 相似文献
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Based on an existing magnesium alloy database, a query system is optimized by considering the design characteristics, and an integrated query system for this magnesium alloy database is developed to meet the demands of more users. The result shows that the new system improves the query efficiency of the magnesium alloy database, especially for queries concerning phase diagrams and the structure of magnesium alloys. This means that the new system provides users a more powerful and useful magnesium alloy database. 相似文献
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【目的】提出适合广西北部湾海洋渔业快速健康发展的科技组织与服务有效模式,为政府提供决策参考。【方法】分析广西北部湾海洋渔业科技组织与服务现状及存在的问题,在此基础上提出完善广西北部湾海洋渔业科技组织与服务的发展策略。【结果】广西北部湾海洋渔业科技组织与服务存在主要职责划分不清、组织内部机构设置不合理、渔业科技服务组织运行机制不完善、渔业科技组织与服务资金投入不足、信息服务网络建设滞后、渔业推广人员素质普遍较低等问题。【建议】应构建适应广西北部湾海洋渔业发展的科技组织与服务体系新模式、设置科学的渔业技术推广体系、拓宽渔业科技组织与服务投资渠道、加强宣传力度、建立发达的渔业资讯网络,以促进广西北部湾海洋渔业持续健康发展。 相似文献
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【目的】明确桂蔬一号黑皮冬瓜氮磷钾施用效应及最高产量与最佳产量施肥量。【方法】采用“311-A”最优混合设计,研究氮、磷、钾组合对桂蔬一号黑皮冬瓜的产量效应,同时建立产量与施肥量数学模型,并对模型进行模拟寻优。【结果】施用氮、磷、钾肥对桂蔬一号黑皮冬瓜均起到增产作用,其肥效表现为：钾肥>磷肥>氮肥;氮磷、钾磷间存在明显的正交互效应,氮钾间存在明显的负交互效应。利用计算机对产量与施肥量数学模型进行摸拟寻优,获得桂蔬一号黑皮冬瓜最高产量（90992.84 kg/ha）的施肥量为N 309.4 kg/ha、P2O5 183.8 kg/ha、K2O 450.0 kg/ha,收益为102061.2元/ha;最佳产量（90906.20 kg/ha）的施肥量为N 168.8 kg/ha、P2O5 183.8 kg/ha、K2O 412.5 kg/ha,收益为103209.6元/ha。【结论】在该试验条件下,钾素是桂蔬一号黑皮冬瓜产量的主要限制因子。由于生产上存在各种变化因素对冬瓜产量的影响,栽培上应注意氮、磷、钾肥的合理搭配,以提高冬瓜产量和品质。生产75000～90000 kg/ha的冬瓜产量,氮、磷、钾优化施用配比应为1.00∶0.70～0.99∶0.86～0.92。 相似文献
36.
灰梨孢菌产孢培养基筛选 总被引：1，自引：0，他引：1
【目的】筛选操作方法简便、产孢效果好、适宜于大多数灰梨孢菌的产孢培养基,为灰梨孢菌研究提供一定的技术支持。【方法】从水稻、香蕉和杂草马唐上分离获得6个灰梨孢菌菌株,接种于紫花鸭趾草、高粱粒和大麦粒等9种培养基上,测定不同培养基对灰梨孢菌产孢量的影响,并比较分析紫花鸭趾草培养基对病菌致病力的影响。【结果】高粱粒、大麦粒和紫花鸭趾草培养基对6个菌株孢子形成均有明显的促进作用,以紫花鸭趾草培养基的促进作用最明显。与常用的高粱粒培养基相比,6个菌株在紫花鸭趾草培养基上形成的病菌分生孢子对香蕉和水稻的致病力没有明显差异。在4℃冰箱中,用紫花鸭趾草培养基保存蕉瘟病和稻瘟病菌菌种,两年后病菌仍具有生活力。【结论】紫花鸭趾草培养基是适宜于灰梨孢菌分生孢子形成的理想培养基,紫花鸭趾草培养基也适合于保存灰梨孢菌。 相似文献
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Long polar fimbriae (Lpf) are recently discovered adhesins and increasingly important genetic markers of pathogenic Escherichia coli strains. The presence and genotype diversity of Lpf operons was screened in a collection of 97 Escherichia coli O157 strains representing different pathotypes, isolated from healthy cattle (n = 43) and human patients (n = 54) in several countries. Individual structural genes of Lpf were scanned by PCR, and allelic variants were detected with a recently developed typing scheme. Ninety-five strains carried at least one whole Lpf operon (genes lpfABCD and/or lpfABCDE). The 64 enterohaemorrhagic (EHEC) and 24 enteropathogenic (EPEC) strains all carried two Lpf operons, allele 3 of lpfA1 and allele 2 of lpfA2, a combination characteristic of the O157:H7/NM serotype. Out of the 9 bovine atypical (AT; stx-, eae-) strains, 7 carried one complete Lpf operon, allele 1 of lpfA2. The atypical strains belonged to main phylogenetic groups A and B1, while the EHEC and EPEC strains were from group D. Lpf variants carried by the 72 strains of the Escherichia coli Reference Collection (ECOR) were determined with the same typing scheme. Alleles were detected in 25 strains, of which 6 were found negative for the respective Lpf operons in earlier studies. The marker value of the Lpf allelic combination for the O157:H7/NM serotype was confirmed, and further evidence was given for the presence of at least two different genetic lineages of atypical bovine E. coli O157 strains. 相似文献
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在阐明发展油橄榄产业重要意义基础上,回顾我国油橄榄产业的发展历程,分析当前发展油橄榄产业面临的主要问题,着重对浙江发展油橄榄产业的基础和条件进行探讨,并进一步提出浙江省油橄榄产业的发展对策。 相似文献
40.
HE Yan song ZHAO Qin XU Zhong ming YANG Zhen dong ZHANG Yuan yuan b 《保鲜与加工》2009,(3):283-287
Noise samples of motorcycles at different engine speeds taken at the position of the ear of a motorcycle operator are selected as the evaluation stimuli to investigate sound preferences of motorcycle sound quality. Subjective testing is carried out via the paired comparison method. Precise calculation of the misjudging rate is adopted to ensure test result reliability. Formulae between subjective preference scores and psychoacoustic parameters are obtained through linear correlation and multi dimensional regression analysis. The results indicate that loudness and roughness are two predominant metrics influencing preference evaluation for motorcycle noise, and the nonlinear equation better describes the dependence of subjective preference scores on psychoacoustic parameters than the linear one. 相似文献