Construction of cDNA Library from Populus euphratica   总被引：1，自引：0，他引：1  

Yu Guangjun Wang Yiqin Shen Xin * College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P.R. China Genetics Institute  Chinese Academy of Sciences  Beijing  P.R. China 《中国林学(英文版)》2003,5(2):7-9

1 Introduction Populus euphratica is a salt tolerant tree species, which is mainly distributed in the desert regions in northwest China (Wei 1993). Moreover, it is the only large tree species, which can survive and develop into forest in these arid and saline-alkali areas. P. euphratica forest plays a very important role in restraining the expansion of desert, maintaining ecological environment in west China, protecting the biological diversity and raising the local people抯 living standard…  相似文献   

Ultrastructural and Extracellular Protein Changes in Cell Suspension Cultures of Populus euphratica Associated with Low Temperature-induced Cold Acclimation   总被引：1，自引：0，他引：1  

Dai Huanqin Lu Cunfu* Zhang Hui Zhang Xujia College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P. R. China Institute of Biophysics  Chinese Academy of Sciences  Beijing  P. R. China 《中国林学(英文版)》2003,5(4)

Populus euphratica Olive is the only tree species that can grow in the saline land and also survive cold winters in northwest China, and it plays a very important role in stabilizing the vulnerable ecosystem there. A cell suspension culture was initiated from callus derived from plantlets of Populus euphratica. Cold acclimation was induced (LT50 of-17.5 ℃) in cell suspension at 4-5 ℃ in the dark for 30 days and the freezing tolerance increased from LT50 of-12.5 ℃ in nonacclimated cells to LT50 of-17.5 ℃ in cold-acclimated cells. Microvacuolation, cytoplasmic augmentation and accumulation of starch granules were observed in cells that were cold-acclimated by exposure to low temperatures. Several qualitative and quantitative changes in proteins were noted during cold acclimation. Antibodies to carrot extracellular (apoplastic) 36 kD antifreeze protein did not cross react on immunoelectroblots with extracellular proteins in cell suspension culture medium of Populus euphratica, indicating no common epitopes in the carrot 36 kD antifreeze protein and P euphratica extracellular proteins. The relationship of these changes to cold acclimation in Populus euphratica cell cultures was discussed.  相似文献   

Analysis on Schistosomiasis Occurrence And Preventive Measures in Three Gorges Reservoir     

SUN Qixiang ZHANG Jianfeng* . Silvicultural Laboratory of State Forestry Administration  Research Institute of Forestry Chinese Academy of Forestry  Beijing  P.R.China . International Center for Bamboo & Rattan  Bamboo & Rattan Laboratory of SFA  Beijing  P.R. China 《中国林业科技(英文版)》2007,6(1):82-86

Recently, due to more flooding, schistosomiasis is in expanding tendency in some areas. With Three Gorges Engineering progress, schistosomiasis will occurr potentially in Three Gorges reservoir. In order to control the disease, some methods must be applied, e.g. chemicals, biological measures. To some extent every way has its specific effect. However, the vital way is improving the environment and reducing the amount of snails. One of the effective ways is conducting forestry ecological engineering that focuses on snail control and schistosomiasis prevention. The practical ways cover such as pollution controlling, ditches surface hardening. Especially for water-level-fluctuation zone, conducting forestry ecological engineering to improve environment to prevent snails＇ propagation is significant and vital. By operating the project environment could be greatly improved and people＇s living conditions would be bettered.  相似文献   

北京地区节水型屋顶绿化植物配置及养护     

王雅楠 巨关升*刘俊祥  杜小娟许新桥 孙振元 《农业科技与信息》2014,(3):7-11

节水型屋顶绿化是北京地区增加城市绿化面积,改善城市生态环境的重要手段。本文通过查阅文献、实地调查和总结分析,从植物生长的条件和配置原则出发,归纳了北京地区屋顶绿化常用的植物材料,提出适合北京地区节水型屋顶绿化的植物选择原则,并根据这些原则,提出了推荐北京地区节水型屋顶绿化使用的乔木植物油松(Pinustabulaeformis)等15种、灌木植物大叶黄杨(Buxusmegistophylla)等27种、地被植物景天类(Sedum spectabilis)等13种,推荐了喷灌、滴灌和渗灌等3种可用于屋顶绿化的节水灌溉技术,并简要介绍了屋顶绿化的修剪要求,为北京地区节水型屋顶绿化的推广提供了理论依据和技术支持。  相似文献   

盐胁迫对甜瓜种子发芽的影响   总被引：2，自引：0，他引：2  

王喜涛  周秀艳  辛明  秦智伟 《北方园艺》2014,(9)

以35个国内外普遍种植的优良甜瓜品种种子为试材,采用培养皿发芽法,研究了不同浓度NaCl胁迫对种子发芽率、发芽势、发芽指数、一级侧根数的影响。结果表明:NaCl胁迫对甜瓜种子的发芽能力有不同程度的抑制作用,随着盐胁迫浓度的增大而抑制加强;50mmol/L NaCl溶液胁迫能够促进种子生长;发芽率、发芽势、发芽指数3个指标综合在一起可作为甜瓜种子耐盐性鉴定的方法,"白糖罐2号"为最耐盐品种,"7号自交系"为最不耐盐品种。  相似文献   

林业常用16种杀虫剂对赤眼蜂的急性毒性和安全性评价   总被引：1，自引：0，他引：1  

徐华强  薛  明*  赵海朋 《林业科技》2014,(2):10-13

采用管测药膜法测定了16种杀虫剂对松毛虫赤眼蜂的急性毒性,并进行安全性评价。急性毒性测定的结果表明:苏云金杆菌、球孢白僵菌、灭幼脲、杀铃脲、甲氧虫酰肼、苦皮藤素对松毛虫赤眼蜂毒性较低,其LR50均高于9.16×10-3mg·cm-2,为低风险性药剂。甲氨基阿维菌素苯甲酸盐、啶虫脒、噻虫啉、辛硫磷、高效氯氟氰菊酯、吡虫啉、氟虫腈和阿维菌素对松毛虫赤眼蜂毒性较高,其LR50分别是2.28×10-4、9.81×10-5、5.23×10-5、2.43×10-5、1.39×10-5、4.33×10-6、2.10×10-6和1.92×10-6mg·cm-2,安全性评价均为高风险性和极高风险性。敌敌畏对松毛虫赤眼蜂的毒性最高,其LR50是1.11×10-6mg·cm-2;其次为虫螨腈,LR50是1.31×10-6mg·cm-2,对松毛虫赤眼蜂伤害均较严重。  相似文献   

热应激诱导大鼠小肠昼夜节律基因差异表达的研究Heat stress induced different circadian rhythms genes expression in rat small intestine     

赵宏  种新禄  陈佳佳  何莎莎  万长荣  刘凤华*ZHAO Hong  CHONG Xinlu  CHEN Jiajia  HE Shasha  WAN Changrong  LIU Fenghua* 《北京农学院学报》2013,(4)

采用石蜡切片H.E染色观察热应激大鼠小肠形态学变化，利用Realtime-PCR技术对小肠中昼夜节律相关基因的表达进行研究。结果：大鼠小肠上皮出现严重损伤，肠黏膜绒毛顶端上皮细胞脱落，固有层裸露；热应激导致Per2和DBP基因瞬时表达极大升高，节律性受到严重影响，Bmal1基因瞬时表达差异不明显，其mRNA表达量峰值后移。To observe the morphological changes in response to heat stress, rat small intestine was embedded in paraffin, sectioned in transverse and stained with hematoxylin and eosin. The expressions of genes related to circadian rhythms were detected by Realtime-PCR. Results: heat stress resulted in profound damage to the epithelium of rat small intestine, the villus tips shed into lumen and the lamina propria was exposed; the gene expressions of Per2 and DBP were significantly increased so after the heat stress and the rhythms was affected; heat stress could not induce Bmal1 different expression immediatley, whereas, the peak of mRNA was hysteretic.  相似文献   

Study of Differentiation and Characteristics of Sieve Elements and P-protein Body in Ginkgo biloba     

LING Yuping * CHEN Peng LI Li WANG Lili PAN Bin TAI Yunlin . Department of Horticulture  College of Agronomy  Yangzhou University  Yangzhou  P. R.China . Department of Plant Science  McGill University    Lakeshore RoadSte. Anne de Bellevue  Quebec  Canada HX V 《中国林业科技(英文版)》2006,5(3):44-51

INTRODUCTION After his study in the change of liver cell in human body, Kerr (1972) discovered Programmed Cell Death (PCD), a cell death model totally different from cell necrotic death, in which the cell necrosis usually appears typically as break of the protoplasm membrane and inflammatory leak of cell inclusions, which is a kind of abnormal process. PCD is a kind of active, physiologic death process. This kind of cell death process can helpthe body to eliminate decrepit, excrescent …  相似文献   

植物低温诱导蛋白和诱导基因研究新进展   总被引：7，自引：0，他引：7  

丁国华  秦智伟周秀艳 《中国农学通报》2003,19(6):33-33

本文就近年来植物低温诱导蛋白和低温诱导基因的研究进展进行了概述，同时探讨了低温诱导蛋白和低温诱导基因与植物抗寒能力之间的关系。  相似文献   

黄瓜的分子标记和连锁图谱研究进展     

丁国华  秦智伟 《勤云标准版测试》2004,(6)

黄瓜分子标记和遗传连锁图谱的研究工作相对番茄、小麦等作物比较落后,至今开展的分子标记研究多围绕黄瓜遗传关系分析进行,现有的几张遗传连锁图谱基本是由美国Staub研究小组完成,已被定位在图谱上的同工酶、RAPD、RFLP、SSR、AFLP等标记总数达到300多个,各标记间平均距离达到2.1cM,被整合在一起的遗传基因不足10个(F、B、de、ll、dm)。  相似文献