Strains of Mycoplasma mycoides subsp. mycoides small colony type isolated in China between 1953 and 1960 show close similarity to strains of the Africa/Australia cluster     

Li Y  Xin J  Gao Y  Zhang J  Nicholas RA  Lin Y 《Veterinary journal (London, England : 1997)》2009,179(1):137-141

In this study, six Chinese strains of Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) isolated between 1953-1960 were analysed and their molecular characteristics compared to those of the African PG1 and Afade strains, the European C305 and 138/5 strains and the closely related caprine M. mycoides subsp.mycoides large colony type Y-goat strain. PCR amplification of long DNA fragments showed that the six Chinese strains, the PG1 strain and the Y-goat strain, just like Afade, did not have the 8.84 kb deletion characteristic of the European strains C305 and 138/5. In comparison, the lppB gene sequence of the six MmmSC Chinese strains was found to be 99% homologous to that of PG1and Afade, but <93% homologous to the Y-goat sequence. The anti-rLppB antiserum reacted with PG1, Y-goat and the six Chinese strains at 67 kDa sites in Western blot, indicating that the lppB gene and its encoding protein exist in the Chinese strains. Multilocus sequence analysis (MLSA) of MmmSC strains from various regions confirmed that the Chinese strains were identical to the African and Australian cluster. This finding was further supported by the outcome of selective primer amplification. Based on these results, it is suggested that CBPP in China may have originated from Australia.  相似文献   

Performance and Microstructure of Ultra Low Ion Permeability Cementitious Materials     

WANG Xin gang  SONG Gu quan  CHEN Li he  ZHANG Ai ping 《保鲜与加工》2009,(5):138-144

Microstructure and macro performance of Ultra Low Ion Permeability Cementitious Materials (ULIPCM) were investigated by Micro hardness, MIP and SEM EDXA. It was shown that the compressive strength of ULIPCM was more than 80 MPa, flexural strength was more than 11.0 MPa, and elastic modulus was in the range of 38.0 to 42.0 GPa at the age of 28 days; chloride diffusion coefficient was lower than 0.8×10－13m2·s－1, conductive charge for 6 hours was lower than 300 coulombs; sulfate attack resistance was good; shrinkage at the age of 28 days was lower than 400×10－6. Compared with ordinary concrete with which thickness of interfacial transition zone is 60 to 100 μm, the thickness of interfacial transition zone of ULIPCM was lower than 30 μm. Penetration paths of corrosive medium were effectively interrupted in ULIPCM. And pore structure parameters, such as porosity and the most probable pore radius of ULIPCM, were also obviously optimized in ULIPCM. Besides, few CH crystals were in the ULIPCM aggregate and the interfacial transition zone of ULIPCM. And the orientation of CH crystals was poor.  相似文献   

Experimental Analysis of Construction Time Space Effect for Large Span and Flat Multi Arch Tunnel     

WU Meng jun  ZHANG Yong xing  JIANG Shu ping  LIU Xin rong  LI Xin tong 《保鲜与加工》2009,(5):54-58

For the large span and flat, together with the interaction effect of construction and disturbance of surrounding rock, the mechanical of large span and flat multi arch tunnel is very complicated, and the time space effect is different from that of two lane multi arch tunnel or separated tunnel. Adopting the “CTSSSRH”, the 3 D dynamic process of the tunnel construction was simulated. Analyzing the rule of surrounding rock displacement and stress with excavation, the time space effect of large span and flat multi arch tunnel was proposed.  相似文献   

Treatment Difficulties and Strategies for Landfill Leachate     

LONG Teng rui  YI Jie  LIN Yu lian  YOU Xin 《保鲜与加工》2009,(1):114-119

The current status of leachate treatment in garbage landfills and its treatment difficulties were elaborated and summarized. Based on the advantages and disadvantages of various landfill leachate treatment technologies, a multi stage treatment composed of pretreatment, bio treatment and deep treatment was suggested as an ideal treatment. A new way to treat landfill leachate was presented. It combines electrochemical and oxidation ditches with sand filtration. The advantages of the new technique were also analyzed.  相似文献   

Communication method for electromechanical equipment on fully mechanized face with wirelessmesh network     

ZHOU Xin  WANG Zhongbin  TAN Chao  LIU Xinhua  JI Rui 《保鲜与加工》2014,(3):108-114

Aiming at the problem that the pre-existing communication condition can’t satisfy the dynamic movement of the electric & mechanical equipment on fully mechanized face, a wireless communication method based on mesh network is proposed. The design of the dual antenna and the dynamic distribution method of channels based on the interference perception which make the limited bandwidth sources can be rationally used. A high throughput routing protocol and a multipath routing control are used for the efficient management of the wireless mesh network. The factors which affect the signal strength are analyzed to research the deployment method of the wireless network adapt to the fully mechanized face. Experiments are carried out and the result shows that the signal strength of the wireless mesh network is -41~-68 dBm and the data transfer rate is above or equal 12 Mbps. Thus, the mesh network can satisfy the requirement of the electric & mechanical equipment and the method is a new way to solve the communication problem on fully mechanized face.  相似文献   

Effects of Bacterial-Feeding Nematode Grazing and Tea Saponin Addition on the Enhanced Bioremediation of PyreneContaminated Soil Using Polycyclic Aromatic Hydrocarbon-Degrading Bacterial Strain     

SUN Mingming  LIU Kuan  ZHAO Yuanchao  TIAN D  YE Mao  LIU Manqiang  JIAO Jiaguo  JIANG Xin 《土壤圈》2017,27(6):1062-1072

As one of the most widely distributed bacterial predators in the soil, the role of bacterivorous nematodes on the enhanced bioremediation of polycyclic aromatic hydrocarbon-contaminated soils is crucial, but remains to be investigated.A microcosm-level study was conducted to examine the effects of bacterial-feeding nematode grazing and tea saponin(TS) addition on bioremediation of a pyrene-contaminated soil enhanced by the polycyclic aromatic hydrocarbon(PAH)-degrading bacterial strain Sphingobium sp.PHE9.After 180 d of incubation, the highest pyrene dissipation(71.3%) was achieved through a combination of Sphingobium sp.PHE9 inoculation with nematode and TS addition.Meanwhile, high counts of culturable PAH-degrading bacteria, soil enzyme activity, and biodiversity indices were observed under the combined treatment, implying that the microbiological function of the contaminated soil was significantly restored.Additionally, the results of Tenax~ extraction with the first-order three-compartment model indicated that rate-limiting factors varied among treatments.The lack of degrading microorganisms was the main rate-limiting factor for the treatments involving TS/nematode addition, and inadequate bioaccessible pyrene was the vital rate-limiting factor in the treatments involving Sphingobium sp.PHE9 inoculation.The proposed combined clean-up strategy proved to be a promising bioremediation technology for aged pyrene-contaminated soils.  相似文献   

茶叶皂角用于提高大豆油-水体系洗脱修复焦化厂土壤中多环芳烃、Cd、Ni的可行性研究     

YE Mao  SUN Mingming  XIE Shanni  LIU Kuan  FENG Yanfang  ZHAO Yu  WAN Jinzhong  HU Feng  LI Huixin  ZONG Lianggang  JIANG Xin 《土壤圈》2017,27(3):452-464

Mixed contaminated brownfield sites have brought serious risks to human health and environmental safety. With the purpose of removing polycyclic aromatic hydrocarbons(PAHs) and heavy metals from a coking plant site, an innovative technology for ex-situ washing was developed in the present work. The combination of 15.0 mL L~(-1) soybean oil and 7.5 g L~(-1) tea saponin proved an effective method to extract co-pollutants from soil. After two consecutive washing cycles, the efficiency rates of removal for 3-, 4-,5(+6)-ring, and total PAHs, Cd, and Ni were approximately 98.2%, 96.4%, 92.3%, 96.3%, 94.1%, and 89.4%, respectively. Meanwhile,as evaluated by Tenax extraction method and metal stability indices, the residual PAHs and heavy metals after consecutive washing mainly existed in the form with extremely low bioaccessibility in the soil. Thus, in the soil after two washing cycles, there appeared limited environmental transfer risk of co-pollutants. Moreover, a subsequent precipitation method with alkaline solution and PAHdegrading strain Sphingobium sp. PHE9 inoculation effectively removed 84.6%–100% of Cd, 82.5%–91.7% of Ni, and 92.6%–98.4% of PAHs from the first and second washing solvents. The recovered solvents also exhibited a high recycling effectiveness. Therefore, the combined cleanup strategy proposed in this study proved environmentally friendly, which also played a major role in risk assessment and management in mixed polluted sites.  相似文献   

Multi scale and multi orientation features for image de noising     

CHEN Jian jun  TIAN Feng chun  QIU Yu  LI Xin li 《保鲜与加工》2010,(8):23-28

An adaptive algorithm for image de noising is proposed based on the multi scale and multi orientation features. The coefficients in different scales and different directions are obtained by image decomposition using the nonsubsampled contourlet transform. Then thresholds functions are adaptively set with these coefficients. The texture of the image information is introduced by using the mean of decomposition scale and the energy of regional. The greater the energy, the more information of the texture while the same decomposition scales, the smaller the threshold is set. On the contrary, the greater the threshold is set. After the de noising and then reconstruction of these coefficients, image de noising is implemented. Compare to the wavelet transform threshold and contourlet transform threshold, the nonsubsampled contourlet transform pick up the image detail better and improve the quality of the image.  相似文献   

Microscopical static properties of the confined fluids in nanometer tubes     

LIU Jun fang  LI Qin  LING Xin li 《保鲜与加工》2010,(8):91-95

The static properties of the confined in the nanometer cylinder tubes are investigated by molecular dynamics simulation. The modified Lennard Jones potential function is adopted to describe the interaction between fluid fluid and fluid solid. In which, the introduced parameter α can be adjusted to change the wetting intensity between the fluids and solid solid wall. Furthermore, the microscopic properties of the fluids in the nanometer tube are characterized by the density distribution, the pair distribution function and the axial velocity distribution. The results show that the interaction strength of the atoms between the fluids and solid wall has a significant influence on the microscopic properties of the fluids as well changes the microscopic state in the different radial region and the motional properties of the atoms consisted of the fluids in the tube.  相似文献   

Mechanical Analysis of Herbaceous Plant Strengthening Slope     

YAN Zhi Xin  SONG Jie  CAI Han cheng  WANG Hou yu 《保鲜与加工》2010,(2):30-34

Since there are the root systems of herbaceous plant in the shallow layer of soil masses of slope, the shallow layer of slope becomes a compound materials made of soil masses and root system.Owing to friction action and tensile resistance action of roots, the shear strength of soil layer with root system is increased.And a comprehensive mechanical analysis of how herbaceous plants strengthen slope was carried out.Not only a mechanical model of interaction between root and soil, but also a quantitative calculation of increase of shear strength and stability coefficient of slope was put forward.As a result, the theoretical basis of herbaceous plant ecological slope protection was provided.  相似文献