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32.
作者应用琼胶电泳法对拟鲌和梭鲈的血清旦白成分进行了研究。研究证明.这两种鱼的血清旦白可分离出6—7个主要区带。几种旦白成分在鱼类不同性周期的各个时期是不恒定的,在产卵时期变化明显。拟鲌和梭鲈血清中几种旦白成分含量的季节变化具有共同规律。 相似文献
33.
长期以来农药的不合理利用已经引发了环境污染、食品安全等一系列问题、探索减少农药投入的生产
模式、构建稻田生物多样性生态控制病虫害的水稻生产模式已经成为稻田可持续发展的重要手段之一。 为了更好
地开展海南稻田生物多样性保护、发挥稻田生态系统服务功能、有必要系统全面地了解农民对稻田生物多样性保护
认知及农药使用情况、我们在海南北部和南部分别开展了调查。 结果显示、调查区大部分稻农对稻田生物多样性概
念不了解、相关知识掌握较少、且仅有极少数农民接受过相关的多样性种植培训或者指导。 在农药使用上、存在过
量使用、盲目使用和跟风使用等一系列不科学问题、直接导致海南稻田生态系统遭到破坏、种植成本骤增、稻农净收
益降低。 通过分析、就海南水稻生产中的农药使用问题提出了意见和建议。 相似文献
34.
Pérez-Sánchez T Balcázar JL García Y Halaihel N Vendrell D de Blas I Merrifield DL Ruiz-Zarzuela I 《Journal of fish diseases》2011,34(7):499-507
A study was conducted to evaluate the probiotic properties of endogenous rainbow trout microbiota against pathogenic Lactococcus garvieae. A total of 335 bacterial strains were isolated from rainbow trout and screened for antagonistic activity against L. garvieae using an agar spot assay. Antagonistic strains were grouped by PCR amplification of repetitive bacterial DNA elements (rep‐PCR) and identified by 16S rRNA gene sequence analysis. The results revealed that the antagonistic strains belonged to the genera Lactobacillus, Lactococcus and Leuconostoc. Further probiotic characteristics, such as specific growth rate, doubling time, resistance to biological barriers, antibiotic resistance, hydrophobicity and production of antimicrobial substances, were also studied. These strains were able to survive low pH and high bile concentrations, showed good adherence characteristics and a broad spectrum of antibiotic resistance. The antagonistic efficacy was maintained after sterile filtration and was sensitive to proteinase K, indicating that proteinaceous extracellular inhibitory compounds were at least partially responsible for pathogen antagonism. Based on these results, these strains should be further studied to explore their probiotic effects in challenge experiments in vivo. This study shows clear evidence that the indigenous trout‐associated microbiota may provide a defensive barrier against L. garvieae. 相似文献
35.
Twelve multiparous, cycling, lactating Holstein-Friesian cows were synchronised with prostaglandin F(2alpha) and treated with either 2.5 mg carazolol or saline. There were no differences between the peripheral blood concentrations of oestradiol or progesterone, but in the cows treated with carazolol the periovulatory surge of luteinising hormone was delayed, and oestrous behaviour was expressed later than in the control cows. 相似文献
36.
37.
At present transgenic plants are globally grown. Availability of a reliable regeneration system predominantly from a single transformed cell is the prerequisites for gene transfer but regeneration is still a key problem 《分子植物育种》2007,5(2):289-290
At present, transgenic plants are globally grown. Availability of a reliable regeneration system predominantly from a single transformed cell is the prerequisites for gene transfer, but regeneration is still a key problem (Wenzel, 2006). The application of genetic modification technology in plants is closely related to the efficiency of the regeneration protocol. Shoot formation is oilen enhanced by the combination of auxins and cytokinins. TDZ (Thidiazuron, N-phenyl-N'-1,2,3-thiodiazol-5-ylurea), a non-purine, has been shown to promote differentiation of organized centers of growth in cultured tissues at much lower concentrations, and shoot regeneration occurs with an efficiency comparable to or greater than that of other cytokinins (Fiola et al., 1990). By addition of TDZ, a series of plants which were difficult to culture in vitro or less sensitive to plants growth regulators obtained somatic embryos and regenerated plants, some of them have become the eminent transformation system for genetic engineering. TDZ has been reported to be very efficient in stimulating adventitious shoot production in several recalcitrant plants. TDZ is considered as a potential growth regulator for in vitro shoot regeneration and somatic embryogenesis of several crops. This review summarized how the new growth regulator TDZ to affect the regeneration of transgenic plants. 相似文献
38.
Development of cytoplasmic male sterile 《分子植物育种》2007,5(2):215-216
Development of cytoplasmic male sterile (CMS) lines with elongated uppermost internode (EUI) trait provides a genetic option to eliminate the use of GA3 in hybrid rice. During the past two decades, extensive work has been carried out on the subject that resulted in identification of several mutants with EUI trait for use in developing cytoplasmic/thermo-sensitive genic male-sterile lines with complete panicle exsertion (Okuno and Kawai, 1978; Rutger and Carnahan, 1981; Shen et al., 1987; Yang et al., 2000; 2002; Gangashetti et al., 2004). Two recessive genes (eui-1 and eui-2) mapped on chromosome 5 and 10 respectively have so far been reported to control the internode elongation in rice (Librojo and Khush, 1986; Yang et al., 2001; Ma et al., 2004). Considerable progress has been made in China in exploiting 'eur gene for development of hybrid rice parental lines (Yang et al., 2000; 2002; Zhang et al., 2002). 相似文献
39.
Garde S Carbonell M Fernández-García E Medina M Nuñez M 《Journal of agricultural and food chemistry》2002,50(23):6752-6757
The effect of the addition of Lactococcus lactis subsp. lactis INIA 415, a strain harboring the structural genes of nisin Z and lacticin 481, on the formation of volatile compounds in Hispánico cheese manufactured with a mesophilic starter or with the mesophilic starter and a thermophilic starter was investigated. Addition of bacteriocin-producing L. lactis subsp. lactis INIA 415 to milk enhanced the formation of 2-methyl-propanal, 2-methylbutanal, 3-methylbutanal, 2-methyl-1-propanol, 3-methyl-1-butanol, 1-octanol, 2-butanone, and 2,3-butanedione. On the other hand, addition of thermophilic starter enhanced the formation of acetaldehyde, ethanol, 3-methyl-2-buten-1-ol, ethyl butanoate, ethyl hexanoate, 2-butanone, and 2,3-butanedione in Hispánico cheese. Stepwise discriminant analysis using the relative abundances of volatile compounds classified cheeses by type of starter, with function 1 related to thermophilic starter and function 2 to bacteriocin producer. 相似文献
40.
Consumption of fruits and vegetables, which are rich in polyphenols, has been associated with a reduced risk of chronic diseases such as cancer. Dietary polyphenols have antioxidant and antiproliferative properties that might explain their beneficial effect on cancer prevention. The aim of this study was to investigate the effects of different pure polyphenols [quercetin, chlorogenic acid, and (-)-epicatechin] and natural fruit extracts (strawberry and plum) on viability or apoptosis of human hepatoma HepG2 cells. The treatment of cells for 18 h with quercetin and fruit extracts reduced cell viability in a dose-dependent manner; however, chlorogenic acid and (-)-epicatechin had no prominent effects on the cell death rate. Similarly, quercetin and strawberry and plum extracts, rather than chlorogenic acid and (-)-epicatechin, induced apoptosis in HepG2 cells. Moreover, quercetin and fruit extracts arrested the G1 phase in the cell cycle progression prior to apoptosis. Quercetin and strawberry and plum extracts may induce apoptosis and contribute to a reduced cell viability in HepG2 cells. 相似文献