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竹产业转型升级离不开科技创新及成果转化。文章依托国家林业和草原局科技成果储备数据库和中央财政林业科技推广示范项目数据库，统计分析了中国竹类研究领域成果储备及2014—2017年中央财政林业科技推广示范项目资助的情况。结果表明：1）竹类研究成果的储备量、立项推广量与竹林资源量三者之间在区域分布上呈明显的一致性；2）研究成果所属领域不同，成果储备数与推广立项数也不同，竹材加工领域储备的科技成果最多，但推广立项数较少，立项比仅为8.04%；竹下经济领域的成果储备数较少，但推广立项数较高，立项比高达90.91%；竹笋培育及加工领域是竹林特色产业，成果储备数和立项数均较高；3）各省份关注的成果领域存在一定的差异，体现了竹产业发展的地区差异性和不均衡性；4）鉴定成果是储备数量和推广立项数量均最多的成果类型，其成果成熟度和创新性均具备较高的水平。 相似文献
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Agrobacteriurn tumefaciens-mediated transformation (AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 gene. We studied the key factors influencing transformation, such as A.tumefaciens strains,bacterial cell volume initially used, co-cultivation time, effective period and concentration of AS. The results shows that the ideal A.tumefaciens strain is AGL-1, the optimum bacterial cell volume initially used is 50-100 μL and co-culture 24 hours will get the most transformants. At co-culture period, AS induction is indispensable. It is beneficial to improve transformation ratio by adding to 200 μmol·L-1 AS at preincubate period and increasing AS concentration to 400-600 μmol·L-1 at co-culture period. It determined that antisense VeFAD2 gene has been integrated into the genome of R. arrhizus by PCR-Southern detection. 相似文献
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