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大豆叶部病害研究的简易离体叶片培养技术 总被引:6,自引:0,他引:6
为寻找简易有效的大豆叶部病害研究方法,利用大豆离体叶片,进行了相关试验.试验结果表明,大豆离体复叶培养30 d,新根长达16.8~41.2 cm,平均根长》24 cm,移至营养杯30 d后,叶色及叶片仍保持完好.经离体叶片法和整株法接种大豆锈病病原菌后,不同品种和不同菌株表达出不同的病斑反应型;接种大豆白粉病病原菌,也同样表达出在不同品种和不同接种浓度条件下,大豆白粉病发生程度不同;两种接种方试验结果一致.本方法试验条件可控、易控,省时,省空间,因此可考虑应用于大豆叶部病害研究的各种试验. 相似文献
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In order to identify the pathogen of Botryosphaeriaceae causing mango stem-end rot, tissue isolation method was used to process mango samples collected from Hainan, Guangxi, Yunnan, and Sichuan Provinces in China, and 58 fungal isolates were obtained. Among them, 39 isolates were belonged to Botryosphaeriaceae, accounting for 67.24%. These isolates were identified based on morphological characteristics and phylogenetic analysis with internal transcribed spacer (ITS) and elongation factor 1 alpha (EF-1α). The results showed that there were four main species of Botryosphaeriaceae, including Neofusicoccum parvum (33.33%), Lasiodiplodia theobromae (30.77%), Botryosphaeria dothidea (28.21%), and L. pseudotheobromae (7.69%). The mango stem-end rot caused by L. pseudotheobroma was firstly reported in China. The virulence of the four species was tested by in vitro inoculation and the results showed that all isolates were pathogenic, among which L. theobromae, L. pseudotheobroma, and N. parvum were the “strong” grade, and B. dothidea was the “middle” grade. 相似文献
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