Renal myxozoanosis in weedy sea dragons, Phyllopteryx taeniolatus (Lacepède), caused by Sinuolinea phyllopteryxa n. sp.     

M M Garner  S D Atkinson  S L Hallett  J L Bartholomew  R W Nordhausen  H Reed  L Adams  B Whitaker 《Journal of fish diseases》2008,31(1):27-35

Renal myxozoanosis was diagnosed histologically in 11 captive, wild caught, adult weedy (common) sea dragons, Phyllopteryx taeniolatus, from three separate public aquaria in the United States. Myxozoan spores were visible in wet mounts of kidney tissue and were associated with renal tubular dilatation and tubular epithelial cell hypertrophy. Light and electron microscopy revealed spore morphology consistent with the genus Sinuolinea. Spores were spheroidal, slightly dorso-ventrally compressed, length (L) 17.1 x width (W) 16.4 x thickness (T) 15.6 microm, with two shell valves joined at a distinct, sinuous sutural ridge, and had two nearly spherical polar capsules, L 5.5 x W 5.0 microm, with five to seven turns of the polar filament. There were no extra-valvular ridges or protrusions. DNA sequencing required the design of three new primers that yielded 1740 bp of 18S ribosomal DNA sequence. The parasite was determined to be novel based on morphological and molecular data, and was given the name Sinuolinea phyllopteryxa after its vertebrate host.  相似文献   

Simulating forest cover change in the northeastern U.S.: decreasing forest area and increasing fragmentation     

Adams  Alison B.  Pontius  Jennifer  Galford  Gillian  Gudex-Cross  David 《Landscape Ecology》2019,34(10):2401-2419

Landscape Ecology - Understanding how the Northern Forest landscape has changed and is likely to change, both in terms of forest extent and forest configuration, has important implications for...  相似文献   

Complementary DNA sequencing: expressed sequence tags and human genome project   总被引：227，自引：0，他引：227  

M D Adams  J M Kelley  J D Gocayne  M Dubnick  M H Polymeropoulos  H Xiao  C R Merril  A Wu  B Olde  R F Moreno 《Science (New York, N.Y.)》1991,252(5013):1651-1656

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.  相似文献   

土传花叶病毒外壳蛋白基因导入小麦的研究   总被引：7，自引：0，他引：7  

庞俊兰  徐惠君  杜丽璞  叶兴国  李连城  辛志勇  马有志  刁爱波  Adams M.J 《中国农业科学》2002,35(7):738-742

 利用基因枪技术 ,将小麦土传花叶病毒外壳蛋白基因CWMV CP1和筛选基因bar导入扬麦 15 8,获得14 5株抗Bialaphos再生植株 ;PCR Southern分析 ,其中 2 1株为阳性植株 ,转化率达到 0 .99% ;T1代植株的PCR Southern、单酶切和双酶切Southern杂交 ,证明外源抗性基因已经完整地整合到小麦基因组中 ;T1代植株分离比CP1+ ∶CP1-为 1∶1.3,偏离孟德尔分离定律 ;T2 代植株总RNA ,RT PCR的试验结果表明CWMV CP1在转录水平上得到了表达  相似文献   

Concentrations of bioactive and immunoactive luteinizing hormone in bovine anterior pituitary tissue     

G E Moss  R P Lemenager  J R Parfet  B M Adams  T E Adams 《Domestic animal endocrinology》1988,5(2):185-190

Tissue concentrations of LH as determined by radioimmunoassay (RIA) may not accurately measure actual changes that could occur in biological activity of the hormone. To examine this possibility, pituitary homogenates from 135 beef cows in various physiological states were analyzed for content of LH by both RIA and an in vitro bioassay. The ratio of biological/immunological active concentrations of LH remained constant (.52 +/- .02) even though tissue concentrations of immunoactive LH differed among groups. Tissue concentrations of bioactive LH were linearly related to, and highly correlated with (P less than .001), tissue concentrations of immunoactive LH. These data indicate that only a fraction of the immunoactive LH in the bovine pituitary is biologically active. However, this fraction does not vary with the reproductive status or plane of nutrition.  相似文献   

Carbonate mineral filtrants with new surfaces reduce alkalinity in seawater and artificial seawater: Preliminary findings     

Gary Adams  Stephen Spotte 《Aquacultural Engineering》1985,4(4):305-311

Silica sand (silica), coral (aragonite), and oyster shell (calcite) were ground to similar particle sizes and placed in seawater and artificial seawater (GP2 Medium). Alkalinity and pH values of the artificial seawater decreased substantially over 24 h when in contact with coral and oyster shell; the effects in seawater were minor. Once alkalinity has been reduced, the maintenance of stable pH at values typical of seawater is made more difficult. The data, which are preliminary, have practical application.  相似文献   

Ultrastructural morphogenesis of salmonid alphavirus 1     

T K Herath  H W Ferguson  K D Thompson  A Adams  R H Richards 《Journal of fish diseases》2012,35(11):799-808

Studies on the ultrastructural morphogenesis of viruses give an insight into how the host cell mechanisms are utilized for new virion synthesis. A time course examining salmonid alphavirus 1 (SAV 1) assembly was performed by culturing the virus on Chinook salmon embryo cells (CHSE‐214). Different stages of viral replication were observed under electron microscopy. Virus‐like particles were observed inside membrane‐bound vesicles as early as 1 h following contact of the virus with the cells. Membrane‐dependent replication complexes were observed in the cytoplasm of the cells, with spherules found at the periphery of late endosome‐like vacuoles. The use of intracellular membranes for RNA replication is similar to other positive‐sense single‐stranded RNA (+ssRNA) viruses. The number of Golgi apparatus and associated vacuoles characterized by ‘fuzzy’‐coated membranes was greater in virus‐infected cells. The mature enveloped virions started to bud out from the cells at approximately 24 h post‐infection. These observations suggest that the pathway used by SAV 1 for the generation of new virus particles in vitro is comparable to viral replication observed with mammalian alphaviruses but with some interesting differences.  相似文献   

Identification of B-cell epitopes on the betanodavirus capsid protein   总被引：1，自引：0，他引：1  

Costa JZ  Adams A  Bron JE  Thompson KD  Starkey WG  Richards RH 《Journal of fish diseases》2007,30(7):419-426

The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1-32, 91-162 and 181-212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181-212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents.  相似文献   

Notes     

Daniel Dean  WM. Stuart  H. O. Werner 《American Journal of Potato Research》1928,5(9):266-274

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Review of Recent literature     

E. V. Hardenburg  WM. Stuart 《American Journal of Potato Research》1928,5(9):274-278

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