Complications of Percutaneous Endoscopic Gastrostomy in Dogs and Cats Receiving Corticosteroid Treatment          下载免费PDF全文

J. Aguiar  Y.M. Chang  O.A. Garden 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):1008-1013

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Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA          下载免费PDF全文

CM Balao da Silva  C Ortega‐Ferrusola  JM Morrell  H Rodriguez Martínez  FJ Peña 《Reproduction in domestic animals》2016,51(1):18-25

To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling of spermatozoa – including sex sorting – as basic ground for oxidative damage. The aim of this study was to disclose whether the flow cytometric sorting procedure increases the production of reactive oxygen species (ROS), and to identify if ROS production relates to DNA damage in sorted spermatozoa using specific flow cytometry‐based assays. After sorting, oxidative stress increased from 26% to 33% in pre‐ and post‐incubation controls, to 46% after sex sorting (p < 0.05). Proportions of DNA fragmentation index post‐sorting were approximately 10% higher (31.3%); an effect apparently conduced via oxidative DNA damage as revealed by the oxyDNA assay. The probable origin of this increased oxidative stress owes the removal of enough seminal plasma due to the unphysiological sperm extension, alongside a deleterious effect of high pressure on mitochondria during the sorting procedure.  相似文献   

The Effect of Oxidative Stress on Thawed Bulk‐Sorted Red Deer Sperm          下载免费PDF全文

L Anel‐López  O García‐Álvarez  I Parrilla  D Del Olmo  MR Fernández‐Santos  AJ Soler  A Maroto‐Morales  JA Ortiz  DV Alkmin  T Tarantini  J Roca  EA Martínez  JM Vazquez  JJ Garde 《Reproduction in domestic animals》2016,51(3):407-414

The aims of this study were to assess the effects of the sex‐sorting process on post‐thaw sperm quality as well as on induced oxidative stress damage (H₂O₂ 0 mm  = H000; H₂O₂ 50 mm  = H050; H₂O₂ 100 mm  = H100) and the protective action of reduced glutathione (GSH) and Trolox, when comparing sorted (BSS) and non‐sorted (NS) red deer spermatozoa incubated at 37°C. Sperm samples from three stags were collected by electroejaculation and frozen. Immediately after thawing, sperm motility was higher (p < 0.05) for NS (59% ± 3.3) than BSS (36.9% ± 5.8) sperm. Furthermore, the percentage of apoptotic sperm was higher (p < 0.05) for BSS (21.6% ± 5.0) than NS sperm (14.6% ± 1.2). The presence of H₂O₂ increased DNA damage in NS (H000 = 4.1% ± 0.9; H050 = 9.3% ± 0.7; and H100 = 10.9% ± 2.3), but not in BSS sperm. However, in the presence of oxidant, GSH addition improved (p < 0.05) sperm motility in both groups of sperm samples as compared to their controls (NS: 44.5 ± 4.8 vs 21.1 ± 3.9 and BSS: 33.3 ± 8.1 vs 8.9 ± 1.8). These results demonstrate that the sperm‐sorting process induces sublethal effects, albeit selecting a sperm population with a chromatin more resistant to oxidative stress than that in non‐sorted sperm. Moreover, addition of GSH at 1 mm may be a good choice for maintaining the quality of stressed sperm samples, unlike Trolox, which inhibited sperm motility.  相似文献   

Gross composition,fatty acid profile and sensory characteristics of Saanen goat milk fed with Cacti varieties     

Karen Luanna Marinho Catunda  Emerson Moreira de Aguiar  Pedro Etelvino de Góes Neto  José Geraldo Medeiros da Silva  José Aparecido Moreira  Adriano Henrique do Nascimento Rangel  Dorgival Morais de Lima Júnior 《Tropical animal health and production》2016,48(6):1253-1259

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Preventive health care of Pony Club horses in rural New South Wales,Australia          下载免费PDF全文

P Buckley  D Buckley  GT Coleman  JM Morton 《Australian veterinary journal》2016,94(8):265-270

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Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis     

Barrouin-Melo SM  Poester FP  Ribeiro MB  de Alcântara AC  Aguiar PH  Nascimento IL  Schaer RE  Nascimento RM  Freire SM 《Research in veterinary science》2007,83(3):340-346

An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.  相似文献   

Determination of nutritive value of forages in south Texas using an in vitro gas production technique     

A. D. Aguiar  L. O. Tedeschi  F. M. Rouquette Jr  K. McCuistion  J. A. Ortega‐Santos  R. Anderson  D. DeLaney  S. Moore 《Grass and Forage Science》2011,66(4):526-540

The objectives of this study were to use the in vitro gas production (IVGP) technique to evaluate the pattern and parameters of anaerobic fermentation of forages from south Texas pastures throughout the year to (i) obtain empirical relationships between the IVGP technique fermentation parameters and chemical composition of the forages and (ii) develop equations to compute total digestible nutrients (TDN). During four consecutive years (2006–2009), forage samples were collected monthly (n = 39) at the King Ranch, TX, and chemical analyses and IVGP were obtained. For 2006, 2007, 2008 and 2009, the average lag times, h, were 6·47 ± 0·54, 7·75 ± 0·65, 7·49 ± 2·01 and 5·44 ± 1·46, and the average ratio of millilitre of gas per milligram of dry matter was 0·41 ± 0·11, 0·34 ± 0·09, 0·34 ± 0·07 and 0·26 ± 0·10 respectively. There was a moderate negative correlation (r = ?0·53) between lignin and neutral detergent fibre (NDF) and a moderate positive correlation (r = 0·58) between crude protein and NDF digestibility. The predicted fractional passage rate (kp) by the large ruminant nutrition system model using the level 2 solution was on average 0·0366 h^?1. The average computed TDN assuming a kp of 0·04 h^?1 was 55·9%. We concluded the IVGP technique may be used to predict TDN values of warm‐season forages.  相似文献   

Metabolic responses of matrinxã, Brycon amazonicus (Spix & Agassiz, 1829), exposed to environmental nitrite     

Ive Marchioni Avilez  Lucia Helena de Aguiar  Tiago Silvestre Hori  Gilberto Moraes 《Aquaculture Research》2013,44(4):596-603

Nitrite is usually found in aquatic environments where nitrification process occurs. This ion can cause several injuries to aquatic species, particularly fish. Nitrite reacts with haemoglobin yielding the non‐functional methaemoglobin, which leads to many physiological consequences such as functional anaemia and supposed hypoxia. Metabolism of the freshwater teleost matrinxã, Brycon amazonicus, exposed to environmental 0.6 mg L^?1 of nitrite N‐NO₂^? for 96 h was studied, and the fermentative/oxidative preference was gauged. Concentrations of glycogen, glucose, lactate, pyruvate and ammonia, plus the activities of lactate, glutamate and malate dehydrogenase were assayed. The exposure resulted in a metabolic profile that allowed inferring the continuity of oxidative metabolism. Catabolism of amino acids prevailed or was apparently exacerbated by inferred branchial injury and consequent impairment of nitrogen excretion. Moreover, the studied enzymes glutamate dehydrogenase, lactate dehydrogenase and malate dehydrogenase from brain and heart were little affected by nitrite. The expected fermentative metabolism due to the high methaemoglobin formation was not observed.  相似文献   

Use of Vaginal Electrical Resistance to Diagnose Oestrus,Dioestrus and Early Pregnancy in Synchronized Tropically Adapted Beef Heifers     

CD Hockey  ST Norman  JM Morton  D Boothby  NJ Phillips  MR McGowan 《Reproduction in domestic animals》2010,45(4):629-636

The primary objective of this study was to determine whether a single measurement of intravaginal electrical resistance (VER), using the commercially available Ovatec^® probe, can discriminate between dioestrus and oestrus in Bos indicus females, which had been treated to synchronize oestrus. Santa Gertrudis heifers (n = 226) received one of three oestrous synchronization treatments: double PGF_2α 10 days apart, 8‐day controlled internal drug release (CIDR) treatment or CIDR pre‐synchronization + PGF_2α 10 days after CIDR removal. The heifers were inseminated within 12 h following observed oestrus, or, if not observed, at a fixed time approximately 80 h, following the last synchronization treatment. They were palpated per rectum for signs of pregnancy 9 weeks after artificial insemination (AI). Vaginal electrical resistance measurements were taken at the completion of synchronization treatments (presumed dioestrus), immediately prior to AI (oestrus), and then at 3 and 9 weeks post‐AI. Mean VER differed between presumed dioestrus and oestrus (113.7 vs 87.4, p < 0.001). The area under the receiver operating characteristics (ROC) curve was 0.925, indicating that VER was highly discriminatory between dioestrus and oestrus. Vaginal electrical resistance at time of AI was negatively associated with odds of conception when all inseminations were included in the analyses [odds ratio (OR) = 0.97; 95% CI 0.95–1.00; p = 0.018], but not when fixed time AIs were excluded (OR = 1.00; 95% CI 0.97–1.03; p = 0.982). Mean VER readings differed between pregnant and non‐pregnant animals at both 3 weeks (120.5 vs 96.7, p < 0.001) and 9 weeks (124.0 vs 100.3, p < 0.001) post‐AI. However, 3‐ and 9‐week VER measurements were not highly discriminatory between pregnancy and non‐pregnancy (area under ROC curve = 0.791 and 0.736, respectively). Mean VER at time of AI for animals diagnosed in oestrus differed between each of the oestrous synchronization treatments (84.7, 73.6 and 78.9, groups 1–3 respectively, p < 0.001). These findings suggest that measurement of VER may improve accuracy of oestrus diagnoses when selecting cattle for AI following oestrous synchronization programmes involving tropically adapted cattle.  相似文献   

Extracellular matrix in epitheliochorial,endotheliochorial and haemochorial placentation and its potential application for regenerative medicine          下载免费PDF全文

D Orechio  BA Aguiar  PO Favaron  MA Miglino 《Reproduction in domestic animals》2017,52(1):3-15

A placenta is defined as structural approximation of maternal and foetal tissues to perform physiological exchange. Associated processes of differentiation and the establishment of its cells take place within the extracellular matrix (ECM) that provides a rich environment of collagens, fibronectins, cytokines and other components. Placental ECM is promising for tissue regeneration purposes, because it has immune tolerance capacities that may cause only minimal rejections of transplants with immunological differences between donor and recipient. However, specific characteristics of ECM during evolution of the structurally very diverse mammalian placenta are not yet revealed. We here address the major aspects of placental types, that is non‐invasive (epitheliochorial), medium (endotheliochorial)‐to‐high (haemochorial) invasive nature of the interhemal barrier between the foetal and maternal blood system as well as their main components of ECM with special reference to species that are commonly used as animal models for human placentation and in the potential applications for regenerative medicine.  相似文献