Activity of glutathione S-transferase toward some herbicides and its regulation by benoxacor in non-embryogenic callus and in vitro regenerated tissues of Zea mays     

Daniele Del Buono  Alvaro Standardi 《Pesticide biochemistry and physiology》2006,85(2):61-67

A procedure was developed to obtain non-embryogenic callus and regenerated lines from root segments of Zea mays grown in aseptic conditions. The activity of glutathione-S-transferases (GSTs), for non-embryogenic callus, was determined toward 1-chloro-2,4-dinitrobenzene (CDNB) and it was compared with that obtained for corn seedlings grown without hormones. For the callus masses, increases of specific activity toward CDNB and the kinetic parameter V_max were observed with respect to corn seedlings. The procedure permitted the regenerating of tissues from callus explants, therefore the GST(CDNB) activity and the effect of the safener benoxacor on its expression were investigated for the regenerated tissues grown in agarized substrate and in liquid medium. These explants showed a constitutive GST(CDNB) activity higher than corn seedlings and this activity was increased, for both tissues, in response to the presence of the safener benoxacor in the growing medium. The GST activity for the above tissues was also assayed toward benoxacor and terbuthylazine, metolachlor and fluorodifen herbicides. Measurable GST activity was found toward some of the above chemicals and it was found to be significantly enhanced in response to benoxacor treatment.  相似文献   

Empirical relationships between land use/cover and estuarine condition in the Northeastern United States   总被引：1，自引：0，他引：1  

Wilfrid Rodriguez  Peter V. August  Yeqiao Wang  John F. Paul  Arthur Gold  Norman Rubinstein 《Landscape Ecology》2007,22(3):403-417

Land–water interactions were examined in three regions in the Virginian Biogeographic Province; the southern shore of Cape Cod, Massachusetts; the Hudson/Raritan region of New York; and the eastern shore of the Delmarva (Delaware/Maryland/Virginia) Peninsula. Cumulative distribution functions were used to evaluate similarity in environmental condition among estuaries. Spatial-setting variables (location in a river, coastal lagoon, or in open waters) were associated with variation for some measures of estuarine condition. Patterns of coastal urban and agriculture gradients were measured and their relationship with indicators of estuarine condition was modeled statistically. When estuaries were pooled, the highest variation explained by spatial-setting variables was found for dissolved oxygen (DO, R ² = 0.44) and salinity (R ² = 0.58), with DO decreasing in river locations and salinity decreasing with rainfall and sampling locations near rivers. The explanatory power for the other indicator variables was low and varied from 6% to 27%. Rainfall explained some of the variation (R ² = 0.23) in total suspended solids. Moderate (0.4 < | r | < 0.7) to strong (| r | ≥ 0.7) linear associations were found between total urban area and measures of estuarine condition. Within regions, total urban area was positively associated with Silver (r = 0.59), Cadmium (r = 0.65), and Mercury (r = 0.47) in Cape Cod, and inversely related to DO (r = −0.65) in the Hudson/Raritan region. No associations were found in the Delmarva Peninsula study area. Total area of agriculture showed a moderate association with Arsenic in Cape Cod, but no other associations were found in the other two regions. Our analyses show a measurable impact of urban land use on coastal ecosystem condition over large areas of the northeastern United States. This pattern was most evident when many different landscapes were considered simultaneously. The relationship between urban development and estuarine condition were weaker within the individual regions studied. The use of land use/cover models for predicting estuarine condition is a challenging task that warrants enhancements in the type, quantity, and quality of data to improve our ability to discern relationships between anthropogenic activities on land and the condition of coastal environments.  相似文献   

Evaluation of a cloned Babesia bovis organism as a live immunogen     

G M Buening  K L Kuttler  S D Rodriguez 《Veterinary parasitology》1986,22(3-4):235-242

A Babesia bovis isolate was cloned by in vitro cultivation and compared to the original cultured isolate for pathogenicity by animal inoculation. Four yearling heifers were inoculated with cloned material and 4 with the original culture. The four animals which received the cloned Babesia showed comparatively minor hematologic changes and no clinical signs. One animal died in the group that received uncloned Babesia and the mean temperature increase and mean reduction in packed cell volume (PCV) was greater in that group. The four animals receiving the cloned material were challenged 100 days following initial inoculation. All of the animals were totally immune with no significant change in temperature or decrease in PCV, whereas control (previously non-inoculated) animals developed significant (P less than 0.001) increases in temperature and severe anemia. The cloned organism appears to be a candidate live immunogen for use in endemic areas to induce protection against bovine babesiosis.  相似文献   

Evaluation,tissue culture propagation,and dissemination of ‘Saba’ and ‘Pelipita’ plantains in Costa Rica     

Robert L. Jarret  Werner Rodriguez  Roberto Fernandez 《Scientia Horticulturae》1985,25(2):137-147

Fruit characteristics of the disease-resistant bluggoe-type (ABB) cooking bananas (Musa × paradisiaca L.) ‘Saba’ and ‘Pelipita’ were compared with those of the susceptible ‘Currare’ (‘Horn’). Yields of ‘Saba’ and ‘Pelipita’ were similar to those of ‘Currare’; however, ‘Saba’ and ‘Pelipita’ yielded fewer hands with a greater number of small fruit when compared with ‘Currare’. Shoot-tip cultures of both clones were readily initiated on a modified Murashige and Skoog (MS) basal medium supplemented with N⁶-benzyladenine (BA) in combination with indole-3-acetic acid (IAA). Propagation cultures were initiated by splitting shoot tips along their longitudinal axis and re-culturing the individual pieces to basal medium supplemented with 5 mg l^?1 BA. Transfer of axillary shoots to hormone-free medium resulted in rapid and extensive root formation. Plantlet survival after transfer to methyl bromide-treated soil exceeded 90%. Establishment in the field was achieved following procedures normally used for vegetative propagation of this crop. Trial plantings of in vitro propagated ‘Saba’ and ‘Pelipita’ were established in various provinces of Costa Rica for grower evaluation and for future comparison of growth and reproductive development with plants of these and other cultivars propagated from corms.  相似文献   

Effects of two calculolytic diets on parameters of feline mineral metabolism          下载免费PDF全文

C. Pineda  E. Aguilera‐Tejero  A. I. Raya  A. Montes de Oca  M. Rodriguez  I. Lopez 《The Journal of small animal practice》2015,56(8):499-504

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Prevalence of internal parasites in beef cows in the United States: Results of the National Animal Health Monitoring System’s (NAHMS) beef study, 2007–2008     

Bert E. Stromberg  Louis C. Gasbarre  Lora R. Ballweber  David A. Dargatz  Judith M. Rodriguez  Christine A. Kopral  Dante S. Zarlenga 《Canadian journal of veterinary research》2015,79(4):290-295

During the United States Department of Agriculture (USDA) National Animal Health Monitoring System’s (NAHMS) 2007–2008 beef study, 567 producers from 24 US States were offered the opportunity to collect fecal samples from weaned beef calves and have them evaluated for the presence of parasite eggs (Phase 1). Participating producers were provided with instructions and materials for sample collection. Up to 20 fresh fecal samples were collected from each of the 99 participating operations. Fresh fecal samples were submitted to one of 3 randomly assigned laboratories for evaluation. Upon arrival at the laboratories, all samples were processed for the enumeration of strongyle, Nematodirus, and Trichuris eggs using the modified Wisconsin technique. The presence or absence of coccidian oocysts and tapeworm eggs was also noted. In submissions where the strongyle eggs per gram exceeded 30, aliquots from 2 to 6 animals were pooled for DNA extraction. Extracted DNA was subjected to genus level polymerase chain reaction (PCR) identification for the presence of Ostertagia, Cooperia, Haemonchus, Oesophagostomum, and Trichostrongylus. In this study, 85.6% of the samples had strongyle type, Nematodirus, and Trichuris eggs. Among the samples evaluated, 91% had Cooperia, 79% Ostertagia, 53% Haemonchus, 38% Oesophagostomum, 18% Nematodirus, 7% Trichuris, and 3% Trichostrongylus. The prevalence of coccidia and tapeworm eggs was 59.9% and 13.7%, respectively.  相似文献   

Effectiveness of current anthelmintic treatment programs on reducing fecal egg counts in United States cow-calf operations     

Louis C. Gasbarre  Lora R. Ballweber  Bert E. Stromberg  David A. Dargatz  Judy M. Rodriguez  Christine A. Kopral  Dante S. Zarlenga 《Canadian journal of veterinary research》2015,79(4):296-302

During the United States Department of Agriculture (USDA) National Animal Health Monitoring System’s (NAHMS) 2007–2008 beef study, producers from 24 states were offered the opportunity to evaluate their animals for internal parasites and for overall responses to treatment with anthelmintics. A lapse of 45 d was required between initial sampling and any previous treatments. Choice of anthelmintic (oral benzimidazoles, and both injectable and pour-on endectocides) was at the discretion of the producer so as not to alter the local control programs. Fresh fecal samples were collected from 20 animals, or from the entire group if less than 20, then randomly assigned to 1 of 3 participating laboratories for examination. Analyses consisted of double centrifugation flotation followed by enumeration of strongyle, Nematodirus, and Trichuris eggs (the presence of coccidian oocysts and tapeworm eggs was also noted). Where strongyle eggs per gram (epg) exceeded 30, aliquots from 2 to 6 animals were pooled for egg isolation and polymerase chain reaction (PCR) analysis for the presence of Ostertagia, Cooperia, Haemonchus, Oesophagostomum, and Trichostrongylus. Results from 72 producers (19 States) indicated that fecal egg count reductions were < 90% in 1/3 of the operations. All operations exhibiting less than a 90% reduction had used pour-on macrocyclic lactones as the anthelmintic treatment. While some of these less than expected reductions could have been the result of improper drug application, PCR analyses of the parasite populations surviving treatment, coupled with follow-up studies at a limited number of sites, indicated that less than expected reductions were most likely due to anthelmintic resistance in Cooperia spp. and possibly Haemonchus spp.  相似文献   

An overview of animal science research 1945–2011 through science mapping analysis     

A. Rodriguez‐Ledesma  M.J. Cobo  C. Lopez‐Pujalte  E. Herrera‐Viedma 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):475-497

The conceptual structure of the field of Animal Science (AS) research is examined by means of a longitudinal science mapping analysis. The whole of the AS research field is analysed, revealing its conceptual evolution. To this end, an automatic approach to detecting and visualizing hidden themes or topics and their evolution across a consecutive span of years was applied to AS publications of the JCR category ‘Agriculture, Dairy & Animal Science’ during the period 1945–2011. This automatic approach was based on a coword analysis and combines performance analysis and science mapping. To observe the conceptual evolution of AS, six consecutive periods were defined: 1945–1969, 1970–1979, 1980–1989, 1990–1999, 2000–2005 and 2006–2011. Research in AS was identified as having focused on ten main thematic areas: ANIMAL‐FEEDING, SMALL‐RUMINANTS, ANIMAL‐REPRODUCTION, DAIRY‐PRODUCTION, MEAT‐QUALITY, SWINE‐PRODUCTION, GENETICS‐AND‐ANIMAL‐BREEDING, POULTRY, ANIMAL‐WELFARE and GROWTH‐FACTORS‐AND‐FATTY‐ACIDS. The results show how genomic studies gain in weight and integrate with other thematic areas. The whole of AS research has become oriented towards an overall framework in which animal welfare, sustainable management and human health play a major role. All this would affect the future structure and management of livestock farming.  相似文献   

Infection dynamics of foot-and-mouth disease virus in pigs using two novel simulated-natural inoculation methods     

C. Stenfeldt  J.M. Pacheco  L.L. Rodriguez  J. Arzt 《Research in veterinary science》2014

In order to characterize foot-and-mouth disease virus (FMDV) infection dynamics in pigs, two simulated-natural inoculation systems were developed and evaluated. Intra-oropharyngeal (IOP) and intra-nasopharyngeal (INP) inoculation both enabled precise control of dose and timing of inoculation while simulating field exposure conditions.  相似文献   

Effects of month of breeding on reproductive efficiency of Holstein cows and heifers inseminated with sex-sorted or conventional semen in a hot environment     

Miguel Mellado  Edgar Sepulveda  Ulises Macias-Cruz  Leonel Avendaño  Jose E. Garcia  Francisco G. Veliz  Alvaro Rodríguez 《Tropical animal health and production》2014,46(1):265-269

The main objective of this study was to assess the effect of month of breeding on reproduction performance of Holstein heifers and cows inseminated with sex-sorted or conventional semen in a hot environment. Pregnancy per artificial insemination (P/AI; 64,666 services over an 8-year period) both in heifers (n?=?22,313) and cows (n?=?42,353) from a large dairy herd in northern Mexico (26°N) were evaluated with the GENMOD procedure of SAS, with respect to month of AI. Overall, P/AI with sex-sorted semen was greater (P?<?0.01) in heifers (41.6 %) than cows (17.3 %). P/AI for cows serviced with conventional semen was 10 % points higher (P?<?0.01) in January and December (31 vs. 21 %) than cows serviced with sex-sorted semen. While there was no difference in P/AI between the sex-sorted sperm and conventional semen in cows inseminated in July (16 and 18 %, respectively), P/AI plummeted for both groups of cows during the summer and fall (more severe heat stress). P/AI was not different between heifers serviced with sex-sorted or conventional semen during the hottest months of the year (July to October). However, during the coldest month of the year (January and February), P/AI was 10 percentage points greater (P?<?0.01) in heifers serviced with conventional than sex-sorted semen. It was concluded that in this hot climate cow and heifer fertility declined in the summer and fall when inseminated with conventional semen. However, the use of sex-sorted semen during summer and fall did not compromise the breeding success in heifers. Thus, this data suggest that sex-sorted semen promotes some embryonic thermoprotective mechanism, which leads to a marginal summer and fall fertility depression with this type of semen in this particular hot environment.  相似文献