Ploidy levels of Dioscorea alata L. germplasm determined by flow cytometry     

J. Obidiegwu  E. Rodriguez  E. Ene-Obong  J. Loureiro  C. Muoneke  C. Santos  M. Kolesnikova-Allen  R. Asiedu 《Genetic Resources and Crop Evolution》2010,57(3):351-356

Dioscorea alata L. is a highly important crop, widely distributed in the humid and semi-humid tropics. Flow cytometry was used to determine the ploidy levels of 74 D. alata genotypes collected mainly from West African countries. Sixty three of the genotypes were found to be tetraploid, one was hexaploid and ten were octoploid. The high percentage of tetraploids together with the small percentage of hexaploid individuals and the absence of diploid individuals gives us some more clues on the possible origin of these species. No association between ploidy level and place of cultivation was found for the tested material. The obtained results represent important knowledge for enhancing the breeding methodologies and optimize germplasm management of this species. It also offers further insights to the phylogeny and evolution of Dioscorea species.  相似文献   

Clues to wood quality and production from analyzing ring width and density variabilities of fertilized Pinus taeda trees     

Ortega Rodriguez  Daigard Ricardo  Tomazello-Filho  Mario 《New Forests》2019,50(5):821-843

Tree growth and wood density are influenced by forest management. Nevertheless, few studies have evaluated their variability responses to fertilizer treatments at inter-annual, inter-tree and stand-production levels. Therefore, the annual ring width (RW) and density (RD) of sixty 17-year-old-Pinus taeda trees fertilized with six doses of composted pulp-mill sludge (CPMS) were analyzed. Ten trees for each treatment were felled and from which wood discs were taken at different trunk heights. The annual RW and RD were provided by X-ray microdensitometry, synchronized and the trunk basic specific gravity (SG_B) and biomass calculated. The effects of CPMS treatments were explored using interaction of variables RW and RD with cambial age, diameter, trunk SG_B and biomass production. Trees treated with CPMS grow faster, increasing their biomass (up to 108%), presenting lower wood density values (significant up to the 6th year) and reaching the mature wood later than untreated trees. Furthermore, the potential use of RW and RD in allometric equations showed good accuracy to predict trunk SG_B and biomass. Altogether, our results indicated that ring width and density revealed the impacts of fertilization treatment on wood quality and production. Our study also provides useful information for forest managers on the fertilization monitoring process.

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Rhodotorulic acid enhances root colonization of tomato plants by arbuscular mycorrhizal (AM) fungi due to its stimulatory effect on the pre-symbiotic stages of the AM fungi     

J.M. Scervino  I. Sampedro  M.A. Ponce  M.A. Rodriguez  J.A. Ocampo  A. Godeas 《Soil biology & biochemistry》2008,40(9):2474

Exudates of Rhodotorula mucilaginosa, a yeast commonly found in the rhizosphere, increased hyphal length of the arbuscular mycorrhizal (AM) fungi Gigaspora rosea and Gigaspora margarita. Rhodotorulic acid (RA), a siderophore compound obtained from R. mucilaginosa exudates, increased hyphal length and branching. Thus, the increase in the number of entry points and the higher AM root colonization of tomato plants in the presence of RA can at least partially be explained by the positive effect of RA on the pre-symbiotic stages of the AM fungi.  相似文献   

Characterization and growth conditions of Vibrio parahaemolyticus strains with different virulence degrees that cause acute hepatopancreatic necrosis disease in Litopenaeus vannamei     

Sonia A. Soto‐Rodriguez  Rodolfo Lozano‐Olvera  Daniel A. Palacios‐Gonzalez  Carmen Bolan‐Mejia  Karla G. Rendon‐Aguilar 《Journal of the World Aquaculture Society》2019,50(5):1002-1015

The phenotypic characteristics and growth kinetics at several temperatures, salinities, and pH values of three Vibrio parahaemolyticus (Vp) strains with different virulence and one nonpathogenic strain were evaluated. Independent of the virulence of the strain, a high metabolic diversity was found, which yielded different colored phenotypes on the CHROMagar? Vibrio. All strains were resistant to ampicillin and carbenicillin, and Vp AHPND+ organisms were the most sensitive to enrofloxacin. The exponential growth of Vp strains started at 1–2 hr of incubation, although no relationship was observed between the bacterial density and degree of virulence. Moreover, the growth of the most virulent strain was independent of the nutrients in the incubation media during the initial hour postinoculation. No strain grew at 4°C in 0% NaCl and pH 4, but only Vp AHPND+ grew at 44°C. For all strains, the lag phase was proportional to the NaCl concentration, and the growth was better at pH 8–9. However, the Vp AHPND? strain displayed a greater variability, was more sensitive to extreme conditions, and showed a lag phase of 9 hr independent of the pH.  相似文献   

The effects of sex and age on phytohaemagglutinin skin-testing of deer   总被引：1，自引：0，他引：1  

Fernandez-de-Mera IG  Vicente J  Hofle U  Rodriguez O  Gaspar-Lopez E  Gortazar C 《New Zealand veterinary journal》2008,56(2):71-73

AIM: To determine if there are sex- or age-related differences in the increase in skinfold thickness in response to the mitogen phytohaemagglutinin (PHA) in red deer. METHODS: One dose of 250 mug PHA was injected intradermally in the right side of the neck, and phosphate buffered saline (PBS) was injected at a second site as a control, in 110 (51 males and 59 females) captive Iberian red deer (Cervus elaphus hispanicus), ranging in age from 21 months to > or =5 years. Skinfold thicknesses were measured immediately before and 72 h following injection. RESULTS: There was a significant effect of gender on the average increase in skinfold thickness; males had greater increases (8.8 (SEM 0.57) mm) than females (4.23 (SEM 0.39) mm) after correcting for other confounding variables. No age-related differences were evident, but differences between sexes were more marked with increasing age. CONCLUSIONS AND CLINICAL RELEVANCE: Effects of gender, probably due to differences in energetic and reproductive constraints in red deer, should be taken into account when interpreting skinfold-test data, both in ecology and in the control of tuberculosis (Tb). Males tend to have a thicker skin than females, so skinfold increase relative to the thickness of the skin, rather than skinfold increase per se, should be used as a more appropriate measure of skinfold increase. This may also have clinical relevance in the interpretation of tuberculin skin testing.  相似文献   

Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain     

Goicoa A  Espino L  Rodriguez I  Puigdemont A  Brazis P  Rejas J 《Acta veterinaria Hungarica》2008,56(2):163-171

Sensitisation to mites is frequent in atopic dogs. The main mite genus involved in canine atopic dermatitis is Dermatophagoides. The importance of storage mite allergens in dogs has been controversial. The aim of this study was to evaluate the sensitisation rates against storage mites (Lepidoglyphus destructor and Tyrophagus putrescentiae) and house dust mites (Dermatophagoides farinae and D. pteronyssinus) in atopic dogs from Galicia, a highly humid and temperate region of Spain, using a FcepsilonRIalpha-based immunoglobulin E (IgE) in vitro test. The study was performed on 95 dogs suffering from atopic dermatitis and presenting detectable specific serum IgE levels: 91.6% of the dogs tested positive for storage mites, whereas sensitisation to house dust mites was detected in 87.4%. These results indicate the importance of storage mites in this specific geographic area.  相似文献   

Preliminary findings in 30 dogs treated with micropulse transscleral cyclophotocoagulation for refractory glaucoma     

John S. Sapienza  Kay Kim  Elena Rodriguez  Nicola DiGirolamo 《Veterinary ophthalmology》2019,22(4):520-528

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Association of macrophage and lymphocyte infiltration with outcome in canine osteosarcoma     

Sita S. Withers  Katherine A. Skorupski  Daniel York  Jin W. Choi  Kevin D. Woolard  Renee Laufer‐Amorim  Ellen E. Sparger  Carlos O. Rodriguez  Stephen J. McSorley  Arta M. Monjazeb  William J. Murphy  Robert J. Canter  Robert B. Rebhun 《Veterinary and comparative oncology》2019,17(1):49-60

Immunotherapeutic strategies have shown promise for the treatment of canine osteosarcoma (cOSA). Very little is known about the immune microenvironment within cOSA, however, limiting our ability to identify potential immune targets and biomarkers of therapeutic response. We therefore prospectively assessed the disease‐free interval (DFI) and overall survival time (ST) of 30 dogs with cOSA treated with amputation and six doses of adjuvant carboplatin. We then quantified lymphocytic (CD3+, FOXP3+) and macrophage (CD204+) infiltrates within the primary tumours of this cohort using immunohistochemistry, and evaluated their association with outcome. Overall, the median DFI and ST were 392 and 455 days, respectively. The median number of CD3+ and FOXP3+ infiltrates were 45.8 cells/mm² (4.6‐607.6 cells/mm²) and 8.5 mm² (0‐163.1 cells/mm²), respectively. The median area of CD204+ macrophages was 4.7% (1.3%‐23.3%), and dogs with tumours containing greater than 4.7% CD204+ macrophages experienced a significantly longer DFI (P = 0.016). Interestingly, a significantly lower percentage of CD204+ macrophages was detected in cOSA arising from the proximal humerus compared to other appendicular bone locations (P = 0.016). Lymphocytic infiltrates did not appear to correlate with outcome in cOSA. Overall, our findings suggest that macrophages may play a role in inhibiting cOSA progression, as has been suggested in human osteosarcoma.  相似文献   

Repeated cross‐sectional study of Trypanosoma cruzi in shelter dogs in Texas,in the context of Dirofilaria immitis and tick‐borne pathogen prevalence     

Carolyn L. Hodo  Jessica Y. Rodriguez  Rachel Curtis‐Robles  Italo B. Zecca  Karen F. Snowden  Kevin J. Cummings  Sarah A. Hamer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(1):158-166

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Pharmacokinetics of meloxicam after oral administration of a granule formulation to healthy horses     

Francisco Javier Mendoza  Juan Manuel Serrano‐Rodriguez  Alejandro Perez‐Ecija 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):961-967

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