Assessment of DNA Damage during In Vitro Development of Buffalo (Bubalus bubalis) Embryos: Effect of Cysteamine     

A Mukherjee  D Kumar  KP Singh  MS Chauhan  SK Singla  P Palta  RS Manik 《Reproduction in domestic animals》2010,45(6):1118-1121

Comet assay was used in the present study to examine DNA damage to buffalo oocytes and embryos during in vitro culture. Embryos were produced in vitro from oocytes obtained from slaughterhouse ovaries in presence of cysteamine (IVM and IVC media supplemented with 50 and 100 μm , respectively) or in its absence (controls). Compared to controls, cysteamine supplementation increased (p < 0.01) cleavage rate and proportion of oocytes that developed to 8‐ to 16‐cell stage. The incidence of DNA damage was lower (p < 0.01) in cysteamine group than that in controls at 8‐ to 16‐ (19.3 ± 4.24 vs 72.0 ± 5.22%) but not in 2‐cell stage embryos (11.7 ± 5.63 vs 20.8 ± 5.49%) or in mature oocytes (5.3 ± 3.43 vs 10.3 ± 4.73%). The tail length, which indicates magnitude of DNA damage, was shorter (p < 0.01) in cysteamine group than in controls in mature oocytes (25.5 ± 0.5 vs 36.0 ± 0.71 pixels) and 8‐ to 16‐cell stage (49.2 ± 1.64 vs 152.7 ± 1.28 pixels) but not in 2‐cell stage embryos (36.3 ± 1.54 vs 36.4 ± 0.75 pixels). Also, exposure of oocytes/embryos to UV radiation or H₂O₂ caused extensive DNA damage. In conclusion, these results suggest that oocytes/embryos suffer from DNA damage during progress of in vitro culture, which can be partly ameliorated by cysteamine supplementation of culture media.  相似文献   

Movement of cyantraniliprole in plants after foliar applications and its impact on the control of sucking and chewing insects          下载免费PDF全文

James D Barry  Hector E Portillo  I Billy Annan  Rachel A Cameron  Donald G Clagg  Robert F Dietrich  Lawrence J Watson  Robert M Leighty  David L Ryan  James A McMillan  R Scott Swain  Raymond A Kaczmarczyk 《Pest management science》2015,71(3):395-403

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Off-axis crustal thickness across and along the east pacific rise within the MELT area   总被引：1，自引：0，他引：1  

JP Canales  RS Detrick  S Bazin  AJ Harding  JA Orcutt 《Science (New York, N.Y.)》1998,280(5367):1218-1221

Wide-angle seismic data along the Mantle Electromagnetic and Tomography (MELT) arrays show that the thickness of 0.5- to 1. 5-million-year-old crust of the Nazca Plate is not resolvably different from that of the Pacific Plate, despite an asymmetry in depth and gravity across this portion of the East Pacific Rise. Crustal thickness on similarly aged crust on the Nazca plate near a magmatically robust part of the East Pacific Rise at 17 degrees15'S is slightly thinner (5.1 to 5.7 kilometers) than at the 15 degrees55'S overlapping spreading center (5.8 to 6.3 kilometers). This small north-south off-axis crustal thickness difference may reflect along-axis temporal variations in magma supply, whereas the across-axis asymmetry in depth and gravity must be caused by density variations in the underlying mantle.  相似文献   

The Establishment of Early Pregnancy in Cows     

DC Wathes  RS Robinson  GE Mann  GE Lamming 《Reproduction in domestic animals》1998,33(3-4):279-284

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Science for all nations     

Annan K 《Science (New York, N.Y.)》2004,303(5660):925

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Corpus Luteum–Endometrium–Embryo Interactions in the Dairy Cow: Underlying Mechanisms and Clinical Relevance     

RS Robinson  AJ Hammond  DC Wathes  MG Hunter  GE Mann 《Reproduction in domestic animals》2008,43(S2):104-112

Conception rates of dairy cows are currently declining at an estimated 1% every year. Approximately, 35% of embryos fail to prevent luteolysis during the first three weeks of gestation. Interactions between the corpus luteum, endometrium and embryo are critical to the successful establishment of pregnancy and inadequacies will result in the mortality of the embryo. For example, as little as a one day delay in the post-ovulatory rise of progesterone has serious consequences for embryo development and survival. Recently, we found that LH support, degree of vascularization and luteal cell steroidogenic capacity were not the major factors responsible for this luteal inadequacy, but are nevertheless essential for luteal development and function. Progesterone acting on its receptor in the endometrium stimulates the production of endometrial secretions on which the free-living embryo is dependent. However, their exact composition and effects of inadequate progesterone remains to be determined. The embryo is recognized through its secretion of interferon tau (IFNT), which suppresses luteolytic pulses of prostaglandin F_2α. In the cow, it is most likely that IFNT inhibits oxytocin receptor up-regulation directly and does not require the prior inhibition of oestrogen receptor α (ESR1). Unravelling the precise luteal-endometrium and embryo interactions is essential for us to understand pregnancy establishment and development of strategies to reverse the declining fertility of dairy cows.  相似文献   

Cattle herd inspections and fly trapping for the detection of the Old World screw‐worm fly (Chrysomya bezziana)     

R Urech  S Muharsini  RS Tozer  Sumartono  PE Green  GW Brown  JP Spradbery  DG Mayer  Y Tack Kan  A Kison 《Australian veterinary journal》2014,92(1-2):28-32

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具抗水稻白叶枯病新基因的新种质及其利用     

孙立华  高东迎  许志刚  陈志谊  朱永兰  胡白石  陆凡  杨安南  刘永锋 《江苏农业学报》2000,16(4)

运用细胞突变体离体筛选技术,以感白叶枯病的杂交稻恢复系"明恢63"的成熟胚为外植体,诱导筛选育成抗白叶枯病突变品系"HX-3".  相似文献   

Lupinosis in yearling cattle     

JT MACKIE  RS RAHALEY  R BENNETT 《Australian veterinary journal》1992,69(7):172-173

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Simulation of the economic impact of proliferative enteritis on pig production in Australia   总被引：2，自引：0，他引：2  

PK HOLYOAKE  BP MULLAN†  RS CUTLER 《Australian veterinary journal》1996,73(3):89-92

The economic impact of proliferative enteritis (PE) on an ‘average’ pig farm was calculated using the AUSPIG decision support system. Inputs were modelled on actual cases of PE, in which affected herds suffered from depressed growth rate, decreased feed efficiency and stock losses. The costs associated with non-haemorrhagic PE and proliferative haemorrhagic enteropathy ranged from $15/sow/yr to $141/sow/yr, respectively, depending on the clinical severity of the disease, incidence of infection and the type of medication strategy used to treat and control the disease.  相似文献