Stress exposure during the preimplantation period affects blastocyst lineages and offspring development     

Ján BURKU?  Martina KA?MAROVá  Janka KUBANDOVá  Natália KOKO?OVá  Kamila FABIANOVá  Du?an FABIAN  Juraj KOPPEL  ?tefan ?IKO? 《The Journal of reproduction and development》2015,61(4):325-331

We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life.  相似文献   

Impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in Kenya     

Nicholas A Lyons  Neal Alexander  Katharina DC St?rk  Thomas D Dulu  Jonathan Rushton  Paul EM Fine 《Veterinary research》2015,46(1)

Foot and mouth disease (FMD) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into FMD-free countries. Information on the impact of the disease in FMDV-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. A FMD (virus serotype SAT2) outbreak on a large-scale dairy farm in Nakuru County, Kenya provided an opportunity to evaluate the impact of FMD on clinical mastitis and culling rate. A cohort approach followed animals over a 12-month period after the commencement of the outbreak. For culling, all animals were included; for mastitis, those over 18 months of age. FMD was recorded in 400/644 cattle over a 29-day period. During the follow-up period 76 animals were culled or died whilst in the over 18 month old cohort 63 developed clinical mastitis. Hazard ratios (HR) were generated using Cox regression accounting for non-proportional hazards by inclusion of time-varying effects. Univariable analysis showed FMD cases were culled sooner but there was no effect on clinical mastitis. After adjusting for possible confounders and inclusion of time-varying effects there was weak evidence to support an effect of FMD on culling (HR = 1.7, 95% confidence intervals [CI] 0.88-3.1, P = 0.12). For mastitis, there was stronger evidence of an increased rate in the first month after the onset of the outbreak (HR = 2.9, 95%CI 0.97-8.9, P = 0.057).

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0173-4) contains supplementary material, which is available to authorized users.  相似文献   

Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption     

Alison Burrells  Julio Benavides  German Cantón  Jo?o L Garcia  Paul M Bartley  Mintu Nath  Jackie Thomson  Francesca Chianini  Elisabeth A Innes  Frank Katzer 《Veterinary research》2015,46(1)

As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety.  相似文献   

Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection     

Fernando Nú?ez-Hernández  Lester J Pérez  Gonzalo Vera  Sarai Córdoba  Joaquim Segalés  Armand Sánchez  José I Nú?ez 《Veterinary research》2015,46(1)

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A novel epidemiological model to better understand and predict the observed seasonal spread of Pestivirus in Pyrenean chamois populations     

Ga?l Beaunée  Emmanuelle Gilot-Fromont  Mathieu Garel  Pauline Ezanno 《Veterinary research》2015,46(1)

Seasonal variations in individual contacts give rise to a complex interplay between host demography and pathogen transmission. This is particularly true for wild populations, which highly depend on their natural habitat. These seasonal cycles induce variations in pathogen transmission. The seasonality of these biological processes should therefore be considered to better represent and predict pathogen spread. In this study, we sought to better understand how the seasonality of both the demography and social contacts of a mountain ungulate population impacts the spread of a pestivirus within, and the dynamics of, this population. We propose a mathematical model to represent this complex biological system. The pestivirus can be transmitted both horizontally through direct contact and vertically in utero. Vertical transmission leads to abortion or to the birth of persistently infected animals with a short life expectancy. Horizontal transmission involves a complex dynamics because of seasonal variations in contact among sexes and age classes. We performed a sensitivity analysis that identified transmission rates and disease-related mortality as key parameters. We then used data from a long-term demographic and epidemiological survey of the studied population to estimate these mostly unknown epidemiological parameters. Our model adequately represents the system dynamics, observations and model predictions showing similar seasonal patterns. We show that the virus has a significant impact on population dynamics, and that persistently infected animals play a major role in the epidemic dynamics. Modeling the seasonal dynamics allowed us to obtain realistic prediction and to identify key parameters of transmission.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0218-8) contains supplementary material, which is available to authorized users.  相似文献   

Ultrastructural characterization of normal Merkel cells in the dog          下载免费PDF全文

Gustavo A. Ramírez  Francisco Rodríguez  Pedro Herráez  Ayoze Castro‐Alonso  Marisa Andrada  Antonio Espinosa‐de‐los‐Monteros 《Veterinary dermatology》2015,26(5):328-e69

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Ocular dimensions,corneal thickness,and corneal curvature in quarter horses with hereditary equine regional dermal asthenia          下载免费PDF全文

Peres R. Badial  Luis Emiliano Cisneros‐Àlvarez  Cláudia Valéria S. Brandão  José Joaquim T. Ranzani  Mayana A.R.V. Tomaz  Vania M. Machado  Alexandre S. Borges 《Veterinary ophthalmology》2015,18(5):385-392

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Assessment of coagulation utilizing thromboelastometry in dogs undergoing orthopedic surgery          下载免费PDF全文

Barbara Bruno DVM  Cristiana Maurella DVM  Sara Falco DVM  Alberto Tarducci DVM  Renato Zanatta DVM  PhD  Paola Gianella DVM  PhD  DACVIM  Antonio D'Angelo DVM  PhD  DECVN  DECBHM  Lisa Piras DVM  PhD  Andrea Di Bella DVM  MRCVS  Antonio Borrelli DVM  PhD  MS 《Journal of Veterinary Emergency and Critical Care》2015,25(3):358-363

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The influence of the obstetrical condition on canine neonatal pulmonary functional competence          下载免费PDF全文

Liege Garcia Silva DVM  PhD  Guilherme Vannucchi Portari PhD  Cristina Fátima Lúcio DVM  PhD  Jaqueline Aguiar Rodrigues DVM  MSc  Gisele Lima Veiga DVM  PhD  Camila Infantosi Vannucchi DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2015,25(6):725-730

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Why cuckoos should parasitize parrotbills by laying eggs randomly rather than laying eggs matching the egg appearance of parrotbill hosts?     

Canchao Yang  Fugo Takasu  Wei Liang  Anders P M?ller 《中国鸟类》2015,(2):53-59

The coevolutionary interaction between cuckoos and their hosts has been studied for a long time, but to date some puzzles still remain unsolved. Whether cuckoos parasitize their hosts by laying eggs randomly or matching the egg morphs of their hosts is one of the mysteries of the cuckoo problem. Scientists tend to believe that cuckoos lay eggs matching the appearance of host eggs due to selection caused by the ability of the hosts to recognize their own eggs.In this paper, we first review previous empirical studies to test this mystery and found no studies have provided direct evidence of cuckoos choosing to parasitize host nests where egg color and pattern match. We then present examples of unmatched cuckoo eggs in host nests and key life history traits of cuckoos, e.g. secretive behavior and rapid egg-laying and link them to cuckoo egg laying behavior. Finally we develop a conceptual model to demonstrate the egg laying behaviour of cuckoos and propose an empirical test that can provide direct evidence of the egg-laying properties of female cuckoos. We speculate that the degree of egg matching between cuckoo eggs and those of the host as detected by humans is caused by the ability of the hosts to recognize their own eggs, rather than the selection of matching host eggs by cuckoos. The case of Common Cuckoos(Cuculus canorus) and their parrotbill hosts(Paradoxornis alphonsianus), where it has been shown that both have evolved polymorphic eggs(mainly blue and white), was used to develop a conceptual model to demonstrate why cuckoos should utilize parrotbill hosts by laying eggs randomly rather than laying eggs matching the appearance of host eggs.In conclusion, we found no evidence for the hypothesis that cuckoos lay eggs based on own egg color matching that of the parrotbill-cuckoo system. We argue theoretically that laying eggs matching those of the hosts in this system violates a key trait of the life history of cuckoos and therefore should be maladaptive.  相似文献