苜蓿地撂荒过程中土壤-微生物-胞外酶及化学计量的变化     

于宗恺  景煜都  刘励  刘小伟  张强  程杰  郭梁 《草地学报》2023,31(4):1115-1124

紫花苜蓿(Medicago sativa L.)作为优良修复草种在黄土高原广泛种植,然而随着农村人口迁移及土地利用变化,苜蓿人工草地大量弃置的问题已引起广泛关注。为研究苜蓿弃置草地土壤质量变化规律,本研究分析了不同弃置年限(0年、5年和10年)苜蓿草地土壤养分、微生物量、参与土壤碳氮磷(C,N,P)循环的胞外酶活性及其化学计量变化。结果表明：总体上,土壤和微生物C,N含量随弃置年限呈增加趋势,而C,N相关胞外酶活性和化学计量变化幅度较小;土壤和微生物P含量随弃置年限增加而显著降低,但P相关胞外酶活性及化学计量均有不同程度的增加。进一步分析发现：相较于其他土层,表层(0～20 cm)土壤C,N,P含量及其酶活性较高,其变化趋势与总体结果相似;亚表层土壤(20～40 cm)和(80～100 cm)深层土壤C,N,P含量及其酶活性呈先增后减趋势,且各指标较于表层土壤均降低40%～80%。相关分析表明弃置草地土壤由于P匮乏而逐步退化,可通过补施磷肥改善。  相似文献   

饲粮添加过瘤胃半胱氨酸对育成期梅花鹿生长性能、营养物质表观消化率和血清生化指标的影响     

杨乾龙  魏倩倩  赵德辉  郭肖兰  张铁涛  王晓旭  鲍坤  王凯英 《草业学报》2023,32(2):148-159

本试验旨在研究饲粮添加过瘤胃半胱氨酸对育成期梅花鹿生长性能、营养物质表观消化率和血清生化指标的影响。采用单因素随机区组试验设计,将12头7月龄雄性梅花鹿仔鹿分为4组(每组3个重复,每个重复1只),组间体重差异不显著。对照组(Ⅰ组)饲喂基础饲粮,试验组分别饲喂额外添加1.25(Ⅱ组)、2.50(Ⅲ组)和5.00 g·kg^-1(Ⅳ组)过瘤胃半胱氨酸(有效成分51.70%)的试验饲粮。试验预饲期15 d,正试期112 d。结果表明,1)与Ⅰ组相比,添加过瘤胃半胱氨酸Ⅲ组总增重和平均日增重显著提高(P<0.05),Ⅲ组料重比显著降低(P<0.05);2)与Ⅰ组相比,添加过瘤胃半胱氨酸各组粗蛋白质、钙和磷表观消化率显著提高(P<0.05),Ⅲ组干物质和有机物表观消化率显著提高(P<0.05);3)与Ⅰ组相比,添加过瘤胃半胱氨酸Ⅱ组和Ⅲ组甘氨酸表观消化率显著提高(P<0.05),各组半胱氨酸、蛋氨酸、丝氨酸、赖氨酸、苏氨酸、缬氨酸、异亮氨酸、亮氨酸、精氨酸、苯丙氨酸、组氨酸、天冬氨酸、酪氨酸、谷氨酸、丙氨酸和脯氨酸表观消化率显著提高(P<0...  相似文献   

Determination of Total Cysteine in Plasma by High Performance Liquid Chromatography and Ultraviolet Detection     

DING Min  HE Jun  YE Lian  CHENG Shu-hui  BAO Hong-yong 《保鲜与加工》2005,(5):116-119

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Feasibility for Building the Under-ground Sewage Treatment Plant in Chongqing City     

LIU Xin-rong  BAO Tai  ZHU Ke-shan 《保鲜与加工》2004,(4):103-106

With the development of the economy, the demand forhabitation is enhanced and the sewage and industrial waster water must be disposed before outlet. Today, most of the sewage treatment plants are built on the aboveground without discussing the underground. Therefore, by the comparative analysis of the property and cost of the underground sewage treatment plant and the ground, the underground sewage treatment plant have merits in save the city land and improvement environment.The Chongqing citypossess a lot of advantages in using the space of underground with the analysis of the physiognomy and geology condition of the Chongqing city. Therefore, the development of underground sewage treatment plant is feasible in the Chongqing city.  相似文献   

玉米免耕破茬室内试验装置的研究设计     

吴仕宏  李宝筏  包文育 《农机化研究》2006,(4):108-109

通过对国内外玉米免耕播种机破茬防堵部件的对比研究,确定采用滚动式破茬犁刀研制出本试验装置,并进行了室内土槽中的实验。该装置测量准确、方便,为研制出适合我国北方垄作玉米免耕破茬防堵部件提供了技术数据。  相似文献   

大中型泵站行星变速传动装置的分析与应用     

鲍培德  马履中  谢俊  尹小琴  杨启志 《中国农村水利水电》2005,(7):77-80

简单地分析了国内大、中型泵站传动装置的现状，讨论了泵站用变速调节装置的作用，并对现有的双速电机调速、液力耦合器和磁力耦合器调速以及变频调速装置进行了分析和讨论，指出了其适用的局限性。提出了一种用新型2X-H型行星齿轮变速箱调节泵速度的方法，给出了3种具体方案。通过对各方案的配齿进行具体计算和分析，得出第2种方案具有行星轮系的结构简单，传动效率高的特点。  相似文献   

利用简易工况法对在用汽车排放检测之探讨     

郭常亮  鲍晓峰  刘汝成  王燕军  李维萍  高松 《农业装备与车辆工程》2007,(5):13-16

在用车尾气排放的治理和监管是中国城市大气环境改善的重点工作之一。利用典型在用车，对国家标准GB18285—2005规定的双怠速法、ASM法、VMAS法和IM195等在用车尾气排放检测方法进行了试验研究，探讨了ASM和VMAS两种检测方法的适用性。  相似文献   

发动机微机控制系统性能测试仪的研究     

李英  何宝文  李晓伟 《拖拉机与农用运输车》2007,34(2):23-25

介绍最新研制的发动机微机控制系统性能测试仪的结构和技术方案。  相似文献   

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D     

XU Jia-qi  AN Mei-ling  YUAN Yue  HAO Yi-fan  WANG Xiu-wen  ZHANG Min  WEI Xiao-wei  LI Hua  WANG Ren-jun 《园艺学报》2021,36(12):2148-2158

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.  相似文献   

大理州“红阳”猕猴桃真菌性枝干的发生与病原鉴定     

贺占雪  姜存良  李欣  包昌艳  王连春  刘惠民 《北方园艺》2021,(4):25-33

以大理州"红阳"猕猴桃枝干为试材,采用田间调查法调查病原菌对其危害状况,对采集的10份病样采用常规组织分离法分离病原,单孢纯化法获得病原菌,采用形态和分子法鉴定病原种类,以期明确大理州"红阳"猕猴桃枝干病害的发生状况及危害病原。结果表明:大理州"红阳"猕猴桃枝干病害发生严重,病株率高达69.6%,5个采样点共分离得到15个菌株,其中以链格孢菌(Alternaria alternata)、厚坦镰刀菌(Fusarium chlamydosporum)、小新壳梭孢菌(Neofusicoccum parvum)和间座壳属中一种(Diaporthe bohemiae)共4类病原菌出现频率最高,是优势种,分离率分别为55.6%、52.4%、49.8%和50.1%。利用柯赫氏法则对分离所得的所有菌株进行致病性测定,证实了优势菌株对猕猴桃茎杆的致病性,进一步明确了引起大理州猕猴桃茎杆的病原真菌。  相似文献