Comments on efforts to eradicate brucellosis     

Kakoma I  Baek BK  Boyle SM  Srianganathan N  Olsen S  Young E 《Journal of the American Veterinary Medical Association》2007,230(1):27

  相似文献   

Identification of dspEF, hrpW, and hrpN loci and characterization of the hrpN Ep gene in Erwinia pyrifoliae     

Rosemary Shrestha  Kenichi Tsuchiya  Su Jin Baek  Hu Nam Bae  Ingyu Hwang  Jang Hyun Hur  Chun Keun Lim 《Journal of General Plant Pathology》2005,71(3):211-220

Erwinia pyrifoliae, the causal pathogen of shoot blight in the Asian pear tree (Pyrus pyrifolia cv. Singo), is host-specific and endemic to Korea. To identify the genes associated with the hypersensitive response (HR) and pathogenicity, a genomic library of E. pyrifoliae WT3 was constructed, and the cosmid clone Escherichia coli (pCEP33) was selected. Sequence analysis of 19.7-kb pCEP33 determined disease-specific (dsp) region homolog and approximately 40% of the hrp genes, which included hrpW, hrpN_Ep, hrpV, hrpT, hrcC, hrpG, hrpF, and partial hrpE homologs, with respect to the cluster of Erwinia amylovora. Additionally, two open reading frames, ORFD and ORFE, were found downstream of the dspEF region. The results of the sequence analysis showed that the pCEP33 did not contain any hrp regulatory genes or most of the genes encoding components of the Hrp protein secretion system. The hrpN_Ep gene of E. pyrifoliae contained five intergenic nucleotide fragment insertions (INFIs) and produced the HR elicitor protein harpin_Ep, with a molecular mass of approximately 44kDa. The purified HrpN_Ep protein elicited faster and stronger HR when infiltrated into tobacco leaves than did HrpN_Ea from E. amylovora. To observe the role of the hrpL gene in the expression of HrpN_Ep, the pEL2 containing hrpL was used to transform E. coli (pCEP33). Expression of HrpN_Ep in E. coli (pCEP33 + pEPL2) was detected with an immunoblot using antiserum raised against HrpN_Ep, indicating a role of hrpL gene in enhancing the expression of HrpN_Ep.  相似文献   

Effects of exposure to genistein during pubertal development on the reproductive system of male mice   总被引：1，自引：0，他引：1  

Lee BJ  Jung EY  Yun YW  Kang JK  Baek IJ  Yon JM  Lee YB  Sohn HS  Lee JY  Kim KS  Nam SY 《The Journal of reproduction and development》2004,50(4):399-409

Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may affect adversely the development of the male reproductive system. Twenty-one days old ICR mice weaned from dams fed with a casein-based AIN-76A diet during gestation and lactation were exposed to genistein (2.5 and 5.0 mg/kg/day, p.o.) for 5 weeks. 17beta-Estradiol (7.5 microg/kg/day) and corn oil were used for the positive and negative vehicle controls, respectively. The animals were fed the casein-based AIN-76A diet throughout the experiment. There were no significant differences in body weights of mice between the genistein groups and the negative control group. No significant differences in relative reproductive organ weights were found among all experimental groups. Sperm counts in epididymis and testes were slightly decreased in the genistein-exposed groups compared with control group. Sperm motile characteristics in genistein-exposed groups were slightly higher than those of the control group. Levels of phospholipid hydroxide glutathione peroxidase mRNA in the testis, epididymis, and prostate of mice exposed to genistein or estradiol were significantly higher than those of the controls (P<0.05). Exposure to genistein caused hyperplasia of Leydig cells in the testis and a slight increase of interstitial fibroblasts in the epididymis, while estradiol treatment caused severe damage to the testis and epididymis. These results suggest that dietary uptake of genistein during the juvenile period may affect male reproductive development, resulting in a slight decrease in sperm count, but with an increase in sperm motion quality.  相似文献   

In vivo alternative testing with zebrafish in ecotoxicology     

Seung-Hyeok Seok  Min-Won Baek  Hui-Young Lee  Dong-Jae Kim  Yi-Rang Na  Kyoung-Jin Noh  Sung-Hoon Park  Hyun-Kyoung Lee  Byoung-Hee Lee  Jae-Hak Park 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):351-357

Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhRE-EGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3'',4,4'',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.  相似文献   

Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis     

Jung-Min Yon  In-Jeoung Baek  Se-Ra Lee  Mi-Ra Kim  Beom Jun Lee  Young Won Yun  Sang-Yoon Nam 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):233-240

Cytoplasmic Cu/Zn superoxide dismutase (SOD1) is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production, allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wide range of pathologies in mice, but little is known about normal SOD1 protein expression in developing embryos. In this study, the expression pattern of SOD1 was investigated in post-implantation mouse embryos and extraembryonic tissues, including placenta, using Western blotting and immunohistochemical analyses. SOD1 was detected in embryos and extraembryonic tissues from embryonic day (ED) 8.5 to 18.5. The signal in embryos was observed at the lowest level on ED 9.5-11.5, and the highest level on ED 17.5-18.5, while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED 13.5-18.5 revealed its ubiquitous distribution throughout developing organs. In particular, high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus, ganglia, sensory cells of the olfactory and vestibulocochlear epithelia, blood cells and vessels, hepatocytes and hematopoietic cells of the liver, lymph nodes, osteogenic tissues, and skin. Thus, SOD1 is highly expressed at late stages of embryonic development in a cell- and tissue-specific manner, and can function as an important antioxidant enzyme during organogenesis in mouse embryos.  相似文献   

Effects of ginsenosides on organogenesis and expression of glutathione peroxidase genes in cultured rat embryos   总被引：2，自引：0，他引：2  

Lee SR  Kim MR  Yon JM  Baek IJ  Lee BJ  Ahn B  Kim YB  Kwack SJ  Lee RD  Kim SS  Cho DH  Hur GH  Yun YW  Nam SY 《The Journal of reproduction and development》2008,54(3):164-170

Ginseng has been extensively used around the world for several thousand years as a food or drug. However, recently, several reports have indicated that the organogenesis of cultured embryos is inhibited by treatment with ginsenoside, the principal component of ginseng. In this study, we evaluated the morphological changes of embryos and the gene expression patterns of antioxidant enzymes, 3 types of glutathione peroxidases [GPx; cytosolic (cGPx), plasma (pGPx) and phospholipid hydroperoxide (phGPx) forms], in cultured rat embryos (embryonic days 9.5-11.5) exposed to ginsenosides Rb1, Rg1, Re and Rc at levels of 5, 50 and 100 microg/ml. With regard to total morphological scores, no significant differences were noted in the embryos exposed to all doses of ginsenosides, with the exception of 50 microg/ml of Rc. In the cultured embryos exposed to Rg1, a majority of the developmental parameters were normal, but growth of the hind- and mid- brains and the caudal neural tube was significantly increased compared with that observed in the control group (P<0.05). Furthermore, Rc significantly enhanced the growth of a variety of developmental parameters in the cultured embryos, with the exception of the hindlimbs. According to the results of our semiquantitative RT-PCR analysis, the levels of cGPx and phGPx mRNA in the cultured embryos were unaffected by treatment with the ginsenosides. However, the levels of pGPx mRNA increased significantly in the embryos treated with ginsenosides Re, Rc and Rb1 compared with the control group (P<0.05). These findings indicate that ginsenosides may exert a stimulatory effect on the growth of embryos via differential expression of GPx genes.  相似文献   

Effect of dietary supplemental medium chain fatty acids instead of antibiotics on the growth performance,digestibility and blood profiles in growing pigs     

Jian Ying Zhang  Dong Heon Baek  In Ho Kim 《Journal of animal physiology and animal nutrition》2019,103(6):1946-1951

Antibiotics have great functions in farm animal. However, the harm of antibiotics can't be ignored. The effects of medium‐chain fatty acids (MCFAs) supplementation to basal diet instead of antibiotics (CSP, Chlortetracycline, sulphonamide dimethazine and procaine penicillin, 1:1:1) on growth performance, nutrient digestibility and blood profile in growing pigs were studied. A total of 140 growing pigs (Landrace × Yorkshire × Duroc) with an average body weight of 27.84 ± 0.42 kg were allotted to four treatments of seven replicates/treatment and five pigs/replicate. The four experimental diets included: CON (basal diet, non‐antibiotic, negative control); CSP (CON + CSP 0.1%, positive control); M1 (CON + MCFA 0.15%) and M2 (CON + MCFA 0.3%). After 5 weeks, the fresh faecal and blood samples were collected from rectum and jugular vein respectively. The average daily gain (ADG) was significantly improved for pigs fed 0.3% MCFAs in relation to basal diet. Meanwhile, CSP supplementation had comparable effect on ADG. The lymphocyte percentage and IgG concentration were higher in blood of pigs‐fed MCFAs in relation to that of CON and CSP treatment while white blood cell and red blood cell were not affected. In relation to basal diet and CSP treatment, the digestibility of dry matter, nitrogen and gross energy (E) were unaffected with MCFAs supplementation. In conclusion, MCFAs improved growth performance on body weight gain and immune profile. Addition 0.3% MCFAs into the diet indicated that its partial positive effect as an alternative to antibiotic.  相似文献   

Canine pyoderma gangrenosum with recurring skin lesions of unknown origin and splenic involvement     

Jung‐Hun Kang  Jung‐Hee Yoon  Yong‐Baek Kim  Cheol‐Yong Hwang 《Veterinary dermatology》2019,30(4):359-e105

  相似文献   

Preface—Biochar and agricultural sustainability     

Wang  Hailong  Baek  Kitae  Xue  Jianming  Li  Yongfu  Beiyuan  Jingzi 《Journal of Soils and Sediments》2020,20(8):3015-3016

Journal of Soils and Sediments -  相似文献   

Image analysis of shatter and pinning events on hard‐to‐wet leaf surfaces by drops containing surfactant     

Olivier DY Huet  Mathieu Massinon  Nicolas De Cock  W Alison Forster  Jerzy A Zabkiewicz  Ravindra Pethiyagoda  Timothy J Moroney  Frederic Lebeau  Scott W McCue 《Pest management science》2020,76(10):3477-3486

  相似文献