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21.
Basso W Venturini MC Moré G Quiroga A Bacigalupe D Unzaga JM Larsen A Laplace R Venturini L 《Veterinary parasitology》2007,144(1-2):157-161
Wallabies and other Australian marsupials are among the most susceptible species to Toxoplasma gondii. Fatal generalized toxoplasmosis was diagnosed in two captive 3 year-old female Bennett's wallabies (Macropus rufogriseus) from Argentina (w 1 and w 2) with a history of sudden death. Both animals had internal joeys which died 2 days after their mothers. Serologically, both females and one adult male without clinical signs from the same enclosure (w 3) had antibody titers for T. gondii>or=800 by the modified agglutination test (MAT); another adult male (w 4) was negative (MAT titer<25). Microscopically, tachyzoites were observed associated to non-suppurative meningoencephalitis, hepatitis, myositis, myocarditis and severe enteritis in hematoxylin and eosin stained sections from both w 1 and w 2. Immunohistochemically, parasites in heart, brain and liver sections of both female wallabies reacted with T. gondii antiserum. T. gondii was isolated from brain tissues of w 1 and w 2 by bioassay in mice and by culture in bovine monocytes and both isolates were cryopreserved. Genomic DNA was isolated from tachyzoites grown in cultures derived from both animals. The primer pair B22/B23 specific for T. gondii produced 115bp amplicons on poliacrylamide electrophoretic gels. Stray cats were suspected as the possible source of infection. Not all infected macropods were ill, showing that the infection may be asymptomatic and is not always fatal. A vertical infection could not be proved in the joey from w 2. As far as we know, this is the first confirmed report of toxoplasmosis in Bennet's wallabies in Argentina. 相似文献
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Sarcocystis sporocysts from the intestines of four opossums (Didelphis albiventris) from Argentina were identified as Sarcocystis falcatula based on schizogonic stages and pathogenicity to budgerigars (Melopsittacus undulatus). Seven budgerigars fed sporocysts from the opossum feces died of acute sarcocystosis 8, 9, 11, 12, and 14 days after inoculation. Schizonts and merozoites found in the lungs and other organs of the budgerigars were identified as S. falcatula based on structure and immunoreactivity with S. falcatula-specific antibody. Sarcocystis falcatula was also isolated in bovine monocyte cell cultures inoculated with lung tissue from a budgerigar that died nine days after ingesting sporocysts. Two budgerigars inoculated subcutaneously with 1,000,000 culture-derived S. falcatula died 11 and 12 days post-inoculation. This is the first report of S. falcatula infection in South America. 相似文献
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Effects of Lactobacillus buchneri as a silage inoculant or probiotic on in vitro organic matter digestibility,gas production and volatile fatty acids of low dry‐matter whole‐crop maize silage 下载免费PDF全文
C. H. S. Rabelo F. C. Basso E. C. Lara L. G. O. Jorge C. J. Härter L. J. Mari R. A. Reis 《Grass and Forage Science》2017,72(3):534-544
Our objective was to investigate Lactobacillus buchneri as a silage inoculant or probiotic on in vitro ruminal measurements of low dry‐matter whole‐crop maize silage. In vitro gas production was conducted using untreated (without inoculant) and inoculated (treated with L. buchneri CNCM I‐4323 at 1 × 105 cfu g?1 of fresh forage) maize silages (wet‐ground) incubated with three different ruminal inocula, in a 2 × 3 factorial arrangement. Ruminal fluids were collected from wethers consuming (i) untreated maize silage (RF‐U); (ii) inoculated maize silage (RF‐I); and (iii) untreated maize silage with a daily dose of L. buchneri CNCM I‐4323 administered directly into the rumen (1 × 107 cfu g?1 of supplied silage [LB‐probiotic]). Gas production was consistently higher when inoculated silage was used as the substrate of fermentation, compared to the untreated silage. When untreated silage was used as substrate, the total volatile fatty acid concentration was higher using RF‐I and LB‐probiotic inocula, compared to the RF‐U inoculum, at 9 hr and at 48 hr of fermentation. It is concluded that L. buchneri should be used as a silage inoculant rather than as a probiotic because it alters fermentation within the silo thereby improving silage quality and enabling some benefits for ruminal fermentation. 相似文献
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Andrew O. Finley Sudipto Banerjee Bruno Basso 《Journal of Agricultural, Biological & Environmental Statistics》2011,16(4):453-474
An objective for applying a Crop Simulation Model (CSM) in precision agriculture is to explain the spatial variability of
crop performance and to help guide decisions related to the site-specific management of crop inputs. CSMs require inputs related
to soil, climate, management, and crop genetic information to simulate crop yield. In practice, however, measuring these inputs
at the desired high spatial resolution is prohibitively expensive. We propose a Bayesian modeling framework that melds a CSM
with sparse data from a yield monitoring system to deliver location specific posterior predicted distributions of yield and
associated unobserved spatially varying CSM parameter inputs. These products facilitate exploration of process-based explanations
for yield variability. The proposed Bayesian melding model consists of a systemic component representing output from the physical
model and a residual spatial process that compensates for the bias in the physical model. The spatially varying inputs to
the systemic component arise from a multivariate Gaussian process, while the residual component is modeled using a univariate
Gaussian process. Due to the large number of observed locations in the motivating dataset, we seek dimension reduction using
low-rank predictive processes to ease the computational burden. The proposed model is illustrated using the Crop Environment
Resources Synthesis (CERES)-Wheat CSM and wheat yield data collected in Foggia, Italy. 相似文献
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Wagner Rodrigo de Souza Nelson Geraldo de Oliveira Felipe Vinecky Ana Paula Ribeiro Marcos Fernando Basso Raphael Augusto das Chagas Noqueli Casari Brbara Andrade Dias Brito da Cunha Karoline Estefani Duarte Thaís Ribeiro Santiago Polyana Kelly Martins Carlos Eduardo Aucique‐Perez Sílvio Carlos Cristofoletti Júnior Alexandre Lima Nepomuceno Carlos Antnio Ferreira de Sousa Adilson Kenji Kobayashi Kazuo Nakashima Kazuko Yamaguchi‐Shinozaki Hugo Bruno Correa Molinari 《Journal of Agronomy and Crop Science》2019,205(6):545-553