Tissue-Specific Variations of Esterase Activities in the Tadpoles and Adults of Pseudis paradoxa (Anura: Hylidae)     

Attademo  Andrés M.  Peltzer  Paola M.  Lajmanovich  Rafael C.  Basso  Agustín  Junges  Celina 《Water, air, and soil pollution》2014,225(3):1-9

Water, Air, &; Soil Pollution - We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α-naphthyl acetate and 4-nitrophenylvalerate) in different...  相似文献   

Generalized Taenia crassiceps cysticercosis in a chinchilla (Chinchilla lanigera)     

Walter Basso  Maja Rütten  Peter Deplazes  Felix Grimm 《Veterinary parasitology》2014,199(1-2):116-120

Taenia crassiceps is a cestode parasite that uses carnivores as definitive hosts and rodents and rabbits as main intermediate hosts, but other animal species and humans may also get infected. One adult male chinchilla (Chinchilla lanigera) from an animal shelter in Switzerland presented widespread subcutaneous fluctuant swellings extended over the forehead, nose, face and thoracic regions with a progressive growth over 3 months. The thoracic swelling was surgically resected, and it consisted of numerous 3–4 mm small transparent vesicles, mainly confined to the subcutaneous tissue, which were morphologically identified as cysticerci of T. crassiceps. The diagnosis was confirmed by PCR and DNA sequence analysis of fragments of the mitochondrial small subunit rRNA and NADH dehydrogenase subunit 1 genes. After 1.5 months, due to enlargement of the swollen areas and deterioration of the general health condition, the chinchilla was euthanized and a necropsy was performed. Thousands of small cysticerci were observed widespread in the subcutis, involving underlying musculature of the whole body, in the thoracic cavity, larynx, pharynx and in the retropharyngeal region. Additionally, three larger metacestodes were detected in the liver and morphologically and molecularly identified as Taenia taeniaeformis strobilocerci. The present case represents an indicator of the environmental contamination with Taenia eggs, highlighting the risk of infection for susceptible animals and humans. Besides the clinical relevance for pets, T. crassiceps is a zoonotic parasite and can be also cause of severe cysticercosis in humans.  相似文献   

First isolation of Neospora caninum from the faeces of a dog from Portugal     

W. Basso  D.C. Herrmann  F.J. Conraths  N. Pantchev  M. Globokar Vrhovec  G. Schares 《Veterinary parasitology》2009

We report the in vitro isolation of Neospora caninum from the faeces of a naturally infected 8-year-old male stray boxer from Portugal. Vero cell cultures were infected using parasite stages obtained after oral inoculation of γ-interferon knockout mice with 10² sporulated oocysts. The isolate was identified by microscopical examination, as well as histological, immunological and molecular methods including a DNA-microsatellite-based typing technique, and was subsequently named NC-P1. The DNA-microsatellite pattern observed in the NC-P1 isolate was not previously reported for any N. caninum isolate. To our knowledge, this is the first isolation of N. caninum from the faeces of a naturally infected dog from Portugal.  相似文献   

Arrhythmogenic right ventricular cardiomyopathy associated with severe left ventricular involvement in a cat     

Paolo Ciaramella DVM  Cristina Basso MD  PhD  Antonio Di Loria DMV  PhD  Diego Piantedosi DMV  PhD 《Journal of Veterinary Cardiology》2009,11(1):41-45

An 8-year-old, 4 kg, intact female, domestic shorthaired cat was referred for tachypnea and pleural effusion. A 24-h Holter recording showed numerous polymorphic ventricular premature complexes with left and right bundle branch block morphology. Echocardiographic examination revealed right atrial and ventricular dilation. The right ventricular free wall was thin and aneurysmal. The cat died 10 days after initiation of antiarrhythmic therapy. Gross and histopathological findings were consistent with arrhythmogenic right ventricular cardiomyopathy (ARVC) associated with severe left ventricular involvement.  相似文献   

Effect of prednisolone therapy on serum levels of 1,2-O-dilauryl-rac-glycero glutaric acid-(6′-methylresorufin) ester lipase in dogs     

Beatriz Mendoza  Maria Joana Dias  Telmo Nunes  Maria Alexandra Basso  Juan Hernandez  Rodolfo Oliveira Leal 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(6):2330-2336

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Quantitative real time polymerase chain reaction assays for the sensitive detection of Besnoitia besnoiti infection in cattle     

Schares G  Maksimov A  Basso W  Moré G  Dubey JP  Rosenthal B  Majzoub M  Rostaher A  Selmair J  Langenmayer MC  Scharr JC  Conraths FJ  Gollnick NS 《Veterinary parasitology》2011,178(3-4):208-216

Bovine besnoitiosis, an economically important disease in cattle in some countries of Africa and Asia, is emerging in Europe. The definitive host of Besnoitia besnoiti, the causative agent of bovine besnoitiosis, is unknown and the transmission of the parasite is not completely understood. Sensitive and quantitative DNA detection methods are needed to determine whether serologically positive animals are infectious and to examine the role of vectors (e.g. haematophagous insects) in the transmission of the parasite. To this end, we established two different 5'-nuclease quantitative assays to detect B. besnoiti infection in cattle and to estimate the parasite load in samples (BbRT1 and BbRT2). These PCRs are based on the sequence of the internal transcribed spacer region 1 (ITS-1) of the ribosomal RNA gene. Tests with serial dilutions of B. besnoiti genomic DNA in a buffer containing 100 ng/μl bovine DNA revealed a detection limit of 0.01 pg genomic B. besnoiti DNA. Reliable quantification was possible in samples containing ≥1 pg B. besnoiti genomic DNA with a coefficient of variation of ≤ 2%. To estimate the diagnostic sensitivity of the tests, skin biopsies and scrapings from the mucous membrane of the vestibulum vaginae (vaginal scrapings) were taken from cattle with clinical signs of chronic besnoitiosis. Regardless of the real time PCR assay used, 90.7% (39/43) of these animals were positive in at least one of two samples (skin or vaginal scrapings). Antibody titers, as determined by an immunofluorescent antibody test, and the threshold cycle values of the real time PCR obtained for skin samples and vaginal scrapings, were significantly correlated. The specificity of the PCRs was confirmed using genomic DNA from related parasites, including genomic DNA of Besnoitia spp., Neospora caninum, Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Isospora spp., Sarcocystis spp., Eimeria bovis, Cryptosporidium parvum, and Trypanosoma brucei brucei. Since the sequence of the ITS-1 region of B. besnoiti is identical with that of Besnoitia species isolated from donkeys (Besnoitia bennetti), and reindeer (Besnoitia tarandi), both real time PCRs detected also DNA of these parasites. One of the B. besnoiti real time PCRs, BbRT1, but not BbRT2, cross-reacted with Besnoitia darlingi, Besnoitia oryctofelisi, and Besnoitia neotomofelis when large amounts of genomic DNA (10 ng) were used. The other B. besnoiti real time PCR assay (BbRT2) was specific for B. besnoiti, B. bennetti and B. tarandi, but did not react when 10 ng DNA of other related parasite species from the genus Besnoitia or other genera were subjected to analysis.  相似文献   

First report of naturally acquired clinical sarcocystosis in a pig breeding stock     

Caspari K  Grimm F  Kühn N  Caspari NC  Basso W 《Veterinary parasitology》2011,177(1-2):175-178

A two-year-old Large White boar from a pig breeding stock in an indoor farm in Switzerland presented anorexia, reduced general condition and fever. Despite antibiotic and anti-inflammatory treatment, the boar developed severe dyspnoea and cyanosis, and died after 4 days. At necropsy, no gross lesions were observed. Histopathologically, multifocal degeneration and necrosis of myocardial fibers with interstitial edema, severe multifocal non-suppurative myocarditis and hepatitis, and non-suppurative interstitial nephritis were observed. In heart samples, groups of organisms resembling apicomplexan tachyzoites were seen associated with the lesions. A PCR using the primers COC1-COC2 that target a conserved region of the small-subunit rRNA gene of Apicomplexa was performed with DNA from paraffin-embedded tissues. An amplification product of about 350 bp was obtained from heart samples. A sequence analysis showed 100% identities with GenBank sequences reported for Sarcocystis miescheriana. The histopathological observations and molecular findings in combination with the clinical signs, and absence of other pathologic agents highly suggested that an acute infection with S. miescheriana was the cause of death in this boar. To our knowledge, this the first report of fatal acute sarcocystosis after natural infection in a pig breeding herd.  相似文献   

First report of the Q biotype of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> in Argentina and Uruguay     

Gabriela Grille  Nathalie Gauthier  José Buenahora  César Basso  Olivier Bonato 《Phytoparasitica》2011,39(3):235-238

Bemisia tabaci adults were collected from pepper and melon at different commercial production greenhouses in Argentina and Uruguay. The biotype status of adults was then established using cytochrome oxidase I gene (mtCOI) as molecular marker. Only the Q biotype was found on all plants sampled. This is the first report of the Q biotype in Argentina and Uruguay.  相似文献   

The contribution of maize cropping in the Midwest USA to global warming: A regional estimate   总被引：2，自引：0，他引：2  

Peter R. Grace  G. Philip Robertson  Neville MillarManuel Colunga-Garcia  Bruno Basso  Stuart H. Gage  John Hoben 《Agricultural Systems》2011,104(3):292-296

Agricultural soils emit about 50% of the global flux of N₂O attributable to human influence, mostly in response to nitrogen fertilizer use. Recent evidence that the relationship between N₂O fluxes and N-fertilizer additions to cereal maize are non-linear provides an opportunity to estimate regional N₂O fluxes based on estimates of N application rates rather than as a simple percentage of N inputs as used by the Intergovernmental Panel on Climate Change (IPCC). We combined a simple empirical model of N₂O production with the SOCRATES soil carbon dynamics model to estimate N₂O and other sources of Global Warming Potential (GWP) from cereal maize across 19,000 cropland polygons in the North Central Region (NCR) of the US over the period 1964-2005. Results indicate that the loading of greenhouse gases to the atmosphere from cereal maize production in the NCR was 1.7 Gt CO₂e, with an average 268 t CO₂e produced per tonne of grain. From 1970 until 2005, GHG emissions per unit product declined on average by 2.8 t CO₂e ha⁻¹ annum⁻¹, coinciding with a stabilisation in N application rate and consistent increases in grain yield from the mid-1970’s. Nitrous oxide production from N fertilizer inputs represented 59% of these emissions, soil C decline (0-30 cm) represented 11% of total emissions, with the remaining 30% (517 Mt) from the combustion of fuel associated with farm operations. Of the 126 Mt of N fertilizer applied to cereal maize from 1964 to 2005, we estimate that 2.2 Mt N was emitted as N₂O when using a non-linear response model, equivalent to 1.75% of the applied N.  相似文献   

A Western apache writing system: the symbols of silas john     

Basso KH  Anderson N 《Science (New York, N.Y.)》1973,180(4090):1013-1022

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