Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum     

DENG Hong  ZOU Fei  GUO Hong-bo 《园艺学报》2005,21(10):1999-2003

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.  相似文献   

田间小棚繁殖烟蚜茧蜂的繁蜂效果研究   总被引：3，自引：0，他引：3  

邓建华  吴兴富  宋春满  黄江梅  刘光辉  杨硕媛 《西南大学学报(自然科学版)》2006,28(1):66-69,73

研究了田间小棚繁殖烟蚜茧蜂的繁蜂效果,比较了在不同接蜂量和蚜量范围条件下的僵蚜数量及其羽化情况。接蜂量和接蜂时的蚜量是影响僵蚜数量的2个重要因素,以接蜂时的蚜量对繁蜂效果的影响更为明显。在接蜂量为蜂(♀)蚜比1∶100、蚜量范围2 000~3 000头/株的条件下,可获得较好的繁蜂效果,僵蚜的数量可达到8 000个/株以上。在繁蜂前期僵蚜的质量较高,僵蚜的羽化率可保持在90%以上,在繁蜂后期受重寄生蜂的影响,僵蚜的质量明显下降。应用田间小棚繁殖烟蚜茧蜂时,重寄生蜂是影响后期繁蜂质量的重要因素。  相似文献   

Changes in Endopeptidase Activity during Photosynthetic Declination in Rice Leaf     

DENG Zhi-rui ZHANG Rong-xian 《水稻科学》2004,11(1)

Two japonica rice varieties, Wuyujing 3 and 97-7, were used to study the changes in contents of soluble protein, free amino acids and endopeptidase activity during photosynthetic declination. The content of soluble protein in flag leaf of cv.Wuyujing 3 was higher than that of cv. 97-7, but decreased rapidly in Wuyujing 3. Free amino acids in flag leaf and the thirteenth leaf of Wuyujing 3 started to increase 10 days before the turning point of photosynthetic declination (TPPD), while it occurred just 1-2 days before TPPD in the flag leaf and the thirteenth leaf of 97-7. During reversible phase of photosynthetic declination,endopeptidase activity remained at a low level and increased slightly only in the later part of this phase. Then it rose up rapidly at irreversible decline phase and reached a very high level. For Wuyujing 3, the change in endopeptidase activity in the thirteenth leaf was parallel to that in flag leaf. However, for 97-7, the rapid increase of endopeptidase activity in the thirteenth leaf started later than that of flag leaf. The results implied that the rate of protein breakdown and conversion to transportable nitrogen in leaves of 97-7 was slower than that in leaves of Wuyujing 3 during photosynthetic declination and it led to relatively lower seed setting rate and fully filling grains rate in 97-7. This may be one of the important reasons why 97-7 could not bring the high yield potentiality into play and the findings may be taken into consideration while breeding for high potential varieties in future.  相似文献   

广义连续格的结构     

邓自克 《湖南农业大学学报(自然科学版)》2001,28(6)

以上（下）子格为工具建立广义连续格的诸结构定理，它们奠定这些格的完全链表现的基础。  相似文献   

江西省主要人工混交林类型的优化与筛选     

陈际伸  吴建福  李勇俊  邓晓媛 《江西林业科技》2001,(Z1)

以杉木或湿地松为主要树种,用南酸枣、檫树、樟树、乐昌含笑、福建柏、鹅掌楸、蓝果树等作伴生树种,开展了13个类型的混交林试验研究,在定性分析筛选的基础上,初选出9个比较优良的混交类型,再依据Ii=P1h/H+P2d/D+P3v/V计算出混交类型的优选指标,用I标准=I单均+aδn计算出类型的优化标准为6.12.据此对参试的混交类型进行优选,选出的最佳混交类型为杉木+南酸枣、杉木+檫树、杉木+樟树和湿地松+鹅掌楸4个混交类型,供林业生产上推广应用.  相似文献   

山区池塘无公害草鱼养殖技术     

邓志武 《水利渔业》2005,25(3):45-47

采取鱼种高密度放养等微流水或半流水、高产高效、无公害养殖草鱼技术模式，于2001年1月至2004年1月在顺昌县洋口镇道吴村和道平自然村实施，通过对典型示范户的抽查，取得甲均单产12891kg／hm^2、年均产值87755元／hm^2、年均毛利49413元／hm^2、投入产出比1：2．02、养殖面积亦从2．8hm^2增至5.7hm^2的成果。2004年1～6月平均每户已轮捕成鱼上市500多kg，平均实现销售收入5000元左右，效益有望进一步提高。  相似文献   

大五星枇杷花期修剪试验初报     

周政华  邓秀玉  文向多  蒋小军 《特产研究》2005,27(1):26-28

春季开花期，根椐大五星枇杷的树势、花量、不同的栽培管理及产量要求等进行花期修剪试验。结果表明：修剪量为10％～20％、去果穗数为30％～35％是比较理想的，既能提高果实的坐果率(30％～70％)、果实的大小(1％～2％)、产量(45％～50％)，又不影响第2年的正常生长与结果。  相似文献   

城市拆迁空地对城市绿化率的影响   总被引：1，自引：0，他引：1  

邓理  郭松青  解清生  高丹  梁应仙 《水土保持研究》2006,13(3):244-246

近年来,我国城市建设发展迅速,产生了大量的拆迁空地,就拆迁空地对城市绿化率的影响进行了定量的研究,并提出相应的对策。  相似文献   

桂东南旅游资源特色及其旅游文化的开发   总被引：2，自引：0，他引：2  

邓飞虎  李少游 《林业调查规划》2006,31(4):51-54,60

旅游文化发展成为近年来旅游业新的增长点,旅游资源文化的正确定位和开发层次是提升旅游产品档次的关键。桂东南旅游资源文化内涵丰富,特色是侨乡风情文化、喀斯特———丹霞地质文化、自然生态文化和名人历史文化等。文章首先阐述旅游与文化的关系,进而论述旅游资源文化的内涵,然后深层次挖掘桂东南旅游资源文化,提出桂东南旅游资源文化发展构想。  相似文献   

氨基酸铁在大鼠小肠中的吸收及组织中沉积研究   总被引：5，自引：0，他引：5  

周桂莲  韩友文  滕冰  徐子伟  刘敏华  徐莹  邓波  徐海风 《动物营养学报》2003,15(3):18-24

本试验用50日龄Wistar纯系雄性大鼠,采用体内原位结扎肠段灌注技术和放射性同位素示踪技术,研究了氯基酸螯合铁(以赖氨酸螯合铁和甘氨酸螯合铁为代表)的吸收特点。试验中观察了向结扎肠段灌注含不同形态铁的吸收液后,不同时间点(5、15、30、60、90、120分钟)血液中59Fe比放射性的动态变化;120分钟(试验结束)时不同组织器官中59Fe的放射性大小和总铁含量以及主要血液学指标的变化。试验结果表明:①赖氨酸和甘氨酸都能促进大鼠对铁的吸收。②与相应摩尔浓度的氨基酸与氯化亚铁的混合物相比,赖氨酸螯合铁和甘氨酸螯合铁能更有效地被大鼠吸收。综合分析本试验结果认为,氨基酸螯合铁都是一种优秀的铁添加剂。从本试验结果看,放射性同位素示踪技术结合结扎十二指肠段灌注技术不失为一种研究动物对微量元素吸收情况的理想试验手段。  相似文献