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Encapsulated Stuphylococcus aureus from bovine mastitis 总被引:1,自引:0,他引:1
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Restriction endonuclease digestions were performed on plasmids purified from Moraxella bovis isolates GRS, Newport, and IBH64. It was determined from single and double digestions of plasmid DNA that GRS and Newport isolates carried 3 large plasmids having molecular sizes of 43.8, 41.3, and 32.8 kilobases (kb). Digestion of the 3 large plasmids and restriction endonucleases Hae III, HindIII, Nde I, and Ava I strongly indicated that these isolates shared structurally identical large plasmids. Timed single digestions with Ava I revealed that the IBH64 isolate carried 2 large plasmids having molecular sizes of 45 and 32.8 kb. The 32.8-kb plasmid was the only large plasmid that appeared to be shared by all 3 M bovis isolates. Two isolates, Newport and IBH64, carried small plasmids in addition to the large plasmids. Restriction maps were constructed for the 43.8-, 41.3-, and 32.8-kb plasmids. 相似文献
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The effect on the early inflammatory response in the bovine mammary gland of a factor extracted from the cell wall of Staphylococcus aureus was studied. The insoluble factor was extracted from a 3h-culture, and was mixed with S. aureus from an overnight culture (OF) for experimental infection of a lactating quarter. Other quarters were infected with a 3h or an overnight culture (O). Five cows were used in the experiment. The O-quarters showed more severe clinical signs than seen in the 3h-and OF-quarters. Antitrypsin levels and NAGase concentrations were much reduced in the 3h-and OF-quarters. While the cell wall factor profoundly reduced the early inflammatory response, the effect on the final outcome of the infection was not determined. 相似文献
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Bird P 《Science (New York, N.Y.)》1988,239(4847):1501-1507
One hypothesis for the information of the Rocky Mountain structures in late Cretaceous through Eocene time is that plate of oceanic lithosphere was underthrust horizontally along the base of the North American lithosphere. The horizontal components of the motion of this plate are known from paleomagnetism, and the edge of the region of flat slab can estimated from reconstructed patterns of volcanism. New techniques of finite-element modeling allow prediction of the thermal and mechanical effects of horizontal subduction on the North American plate. A model that has a realistic temperature-dependent rheology and a simple plane-layered initial condition is used to compute the consequences of horizontal underthrusting in the time interval 75 million to 30 million years before present. Successful prediction of this model include (i) the location, amount, and direction of horizontal shortening that has been inferred from Laramide structures; (ii) massive transport of lower crust from southwest to northeast; (iii) the location and timing of the subsequent extension in metamorphic core complexes and the Rio Grande rift; and (iv) the total area eventually involved in Basin-and-Range style extension. In a broad sense, this model has predicted the belt of Laramide structures, the transport of crust from the coastal region to the continental interior, the subsequent extension in metamorphic core complexes and the Rio Grande rift, and the geographic region of late Tertiary Basin-and-Range extension. Its principal defects are that (i) many events are predicted about 5 million to 10 million years too late and (ii) the wave of crustal thickening does not travel far enough to the east. Reasonable modifications to the oceanic plate kinematics and rheologies that were assumed may correct these defects. The correspondence of model predictions to actual geology is already sufficiently close to show that the hypothesis that horizontal subduction caused the Laramide orogeny is probably correct. The Rocky Mountain thrust and reverse faults formed in an environment of east-west to northeast-southwest compressive stress that was caused by the viscous coupling between the oceanic plate and the base of the North American crust. Nonuniform crustal thickening by simple-shear transport also caused relative uplifts; therefore, this model is consistent with both of the range-forming mechanisms that have been inferred (1). A new proposal that arises from this simulation is that horizontal subduction also caused the subsequent extensional Basin-and-Range taphrogeny by stripping away the mantle lithosphere so that the crust was exposed to hot asthenosphere after the oceanic slab dropped away. 相似文献
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