Serologic responses of calves to sequential infections with epizootic hemorrhagic disease virus serotypes     

R A Bowen 《American journal of veterinary research》1987,48(10):1449-1452

Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (EHDV) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to EHDV after primary inoculation and was removed from the study. Viremia after primary inoculation was transient. Although each infected calf developed a high serum neutralizing antibody titer to EHDV, at no time after inoculation with one or both viruses was antibody detected that neutralized any US serotypes of bluetongue virus. After exposure to both serotypes of EHDV, 4 of 5 calves developed antibodies that cross-reacted with group-specific bluetongue virus antigens.  相似文献   

Extension of the prepatent period of Fasciola hepatica in infected animals following treatment with triclabendazole     

G Büscher  F L Bowen  M B Strong  J R Allison  R J Richards 《The Veterinary record》1987,120(19):460-461

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Embryo transfer from cattle infected with bluetongue virus   总被引：1，自引：0，他引：1  

R A Bowen  T H Howard  R P Elsden  G E Seidel 《American journal of veterinary research》1983,44(9):1625-1628

Embryos recovered nonsurgically from donor cattle during the peak of bluetongue viremia were surgically transferred to seronegative recipients 7 to 8 or 10 to 11 days after the onset of donor estrus. Virus was isolated from the uterine flushing medium recovered from 11 of the 20 donors. Bluetongue virus was not isolated from the blood of any of 39 recipients, nor did any recipient seroconvert to the virus following transfer. The number of recipients that became pregnant after transfer of embryos from infected donors (21 of 39) was not significantly different from contemporary controls. Virus antigen was not detected by immunofluorescence in any of 63 embryos and oocytes recovered from viremic donors. These results indicate that under standard embryo transfer conditions, transmission of bluetongue virus from infected donors to uninfected recipients is unlikely to occur.  相似文献   

Effect of tissue temperature on ulnar nerve conduction velocity in the dog.     

A F Lee  J M Bowen 《American journal of veterinary research》1975,36(9):1305-1307

The motor conduction velocity of the ulnar nerve of 19 mature dogs anesthetized with pentobarbital was electromyographically determined before and at tissue temperature decrements of 2 degrees (C) during cooling of the forelimb. Precooling (37 C) conduction velocity was 56 +/- 7.6 m/second (mean +/- standard deviation). At a tissue temperature of 20 C, conduction velocity was reduced to 31 +/- 6.3 m/second. Regression analysis indicated that conduction velocity decreased linearly by 1.7 m/second for each degree of decrease in tissue temperature between 37 and 20 C.  相似文献   

Acute pancreatitis in two dogs given azathioprine and prednisone   总被引：1，自引：0，他引：1  

K A Moriello  D Bowen  D J Meyer 《Journal of the American Veterinary Medical Association》1987,191(6):695-696

Acute pancreatitis was diagnosed in 2 dogs given azathioprine and prednisone. Prednisone and azathioprine had been given as immunosuppressive therapy for pemphigus foliaceus in dog 1 and for polymyositis in dog 2. Azathioprine was discontinued in both dogs. In dog 1, prednisone was reinstituted on day 6 of hospitalization. Prednisone was continued throughout the period of hospitalization in dog 2. Both dogs recovered without complication. Glucocorticoid therapy has been associated with the development of pancreatitis. In human beings, a common side effect of azathioprine is the development of drug-induced pancreatitis. Definitive identification of azathioprine as the cause of pancreatitis in these dogs was not possible; the owners refused to permit retreatment with the drug. Therefore, the synergistic action between these 2 drugs could not be ruled out as the cause of pancreatitis.  相似文献   

A subacute toxicity study on 99m Tc stannous Glucoheptonate injection.          下载免费PDF全文

L Belbeck  B M Bowen  J Jeu    M Richardson 《Canadian journal of veterinary research》1981,45(3):299-303

A subacute toxicity study on 99m Tc stannous glucoheptonate was performed with rats, dogs and rabbits, injected intravenously at ten to 100 times the human dose on a body weight basis. There were no abnormalities in the clinical status of any of the animals. No changes were found in urinalysis, blood chemistry or hematology in the rabbit nor in gross examination, renal histology or bone marrow smears in the rats and rabbits. Hepatic histology was also done. A focal area of necrosis in a liver of one rabbit that had been injected with 100 times the human dose was observed using light microscopy. Examination by electron microscopy in another group of rats and rabbits was prompted by the observation of that lesion. This revealed vacuolated and dilated smooth endoplasmic reticulum and degranulated and vesiculated rough endoplasmic reticulum in all the test livers. X-ray microanalysis indicates that the ultrastructural changes are linked to the deposition of tin.  相似文献   

Productivity of individual cranberry uprights in Washington and British Columbia     

G.W. Eaton  A.Y. Shawa  P.A. Bowen 《Scientia Horticulturae》1983,20(2):179-184

Yield-component partitioning of the individual cranberry upright (fruiting shoot) was undertaken using a model which included upright length, number of leaves, flowers, berries and seeds, and fruit weight. Repeat cropping of the upright during the following year was also modelled and tested. Fruit set was a major component of yield, but its action was sometimes indirect. Less important components were the number of seeds per berry, berry enlargement per seed, and upright length. Measures of antagonism of “fruiting” to floral initiation were obtained which partially explain the biennial bearing habit of uprights.  相似文献   

Use of goose red blood cells for detection of infection with psittacine beak and feather disease virus by haemagglutination and haemagglutination inhibition     

N SEXTON  WJ PENHALE  SL PLANT  DA PASS 《Australian veterinary journal》1994,71(10):345-347

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Fertility of stallion spermatozoa isolated on albumin gradients     

J.A. Pruitt MS  J.L. Kreider PhD  G.D. Potter PhD  J.M. Bowen MS  DVM  J.W. Evans PhD 《Journal of Equine Veterinary Science》1988,8(2)

Fertility of stallion semen extended with bovine serum albumin (BSA) sucrose extender or cream-gelatin extender was compared. Pregnancy rates were 95% of 47 mares and 86% of 46 mares inseminated with BSA-sucrose and creamgelatin extended semen, respectively. Foaling rates and cycles per conception were not significantly different between treatment groups.Semen from 5 mature stallions was used in an attempt to isolate a population of highly motile spermatozoa. Immediately following collection, samples were evaluated for motility and forward movement. Seven to 10 ml of semen, extended 1:1 with BSA-sucrose extender, were pipetted onto BSA medium separation columns. After 1 hour incubation at 37°C, the top, middle and bottom layers were separately withdrawn from each separation column and pooled, respectively. A marked decrease (P<.001) was noted in the mean motility of spermatozoa in the top layer (35%) as compared to the mean pre-incubation motility (59%). Spermatozoa from middle and bottom layers were significantly (P<.001) more motile (70.6 and 87%) than those from the top layer and pre-incubation samples. Rate of forward movement (RFM) of spermatozoa in lower fractions was higher (P<.001) than RFM of spermatozoa in the top layer. Concentration of spermatozoa decreased (P<.001) as the concentration of BSA in the medium increased.  相似文献   

Use of the partial farm budget technique to predict the economic impact of the flock management decision to use B-mode ultrasonographic pregnancy diagnosis     

D J Sprecher  W B Ley  W D Whittier  J M Bowen  C D Thatcher  K D Pelzer  J M Moore 《Journal of the American Veterinary Medical Association》1989,195(2):199-204

A computer spreadsheet was developed to predict the economic impact of a management decision to use B-mode ultrasonographic ovine pregnancy diagnosis. The spreadsheet design and spreadsheet cell formulas are provided. The program used the partial farm budget technique to calculate net return (NR) or cash flow changes that resulted from the decision to use ultrasonography. Using the program, either simple pregnancy diagnosis or pregnancy diagnosis with the ability to determine singleton or multiple pregnancies may be compared with no flock ultrasonographic pregnancy diagnosis. A wide range of user-selected regional variables are used to calculate the cash flow changes associated with the ultrasonography decisions. A variable may be altered through a range of values to conduct a sensitivity analysis of predicted NR. Example sensitivity analyses are included for flock conception rate, veterinary ultrasound fee, and the price of corn. Variables that influence the number of cull animals and the cost of ultrasonography have the greatest impact on predicted NR. Because the determination of singleton or multiple pregnancies is more time consuming, its economic practicality in comparison with simple pregnancy diagnosis is questionable. The value of feed saved by identifying and separately feeding ewes with singleton pregnancies is not offset by the increased ultrasonography cost.  相似文献