Efficiency of inspection procedures for the detection of tuberculous lesions in cattle   总被引：2，自引：0，他引：2  

LA CORNER  L. MELVILLE†  K. McCUBBIN‡  KJ SMALL†  BS McCORMICK§  PR WOOD  JS ROTHEL 《Australian veterinary journal》1990,67(11):389-392

The sensitivity of the abattoir inspection procedure introduced for Australian export beef in 1976 was compared to a detailed necropsy procedure for the detection of tuberculous lesions in cattle. In a sample of cattle that were reactors to the tuberculin test, abattoir inspection failed to detect an estimated 47% of cattle with lesions. The detailed necropsy examination of cattle with lesions of tuberculosis identified 21 sites of infection compared with 13 to 18 in cattle examined by routine meat inspection procedures. Of the lesions detected during detailed necropsy, 15.9% did not involve the thoracic cavity or the medial retropharyngeal lymph nodes. The failure to detect lesions during abattoir inspection has its greatest significance in an animal with a single lesion. If the 245 cattle found with single lesions during detailed necropsy had been examined by abattoir inspection using the 1976 or the 1986 procedures, 0.8 and 8.9%, respectively, of these animals would not have been detected because the diseased tissues would not have been examined. If meat inspection is to provide an effective means of monitoring the level of bovine tuberculosis during the final stages of eradication, a procedure no less sensitive than that introduced in 1976 should be used.  相似文献   

Proteins Associated With the Early Intrauterine Equine Conceptus     

MA Hayes  BA Quinn  ND Keirstead  P Katavolos  RO Waelchli  KJ Betteridge 《Reproduction in domestic animals》2008,43(S2):232-237

A critical period of early gestation in the mare involves the immobilization (fixation) of the encapsulated conceptus at around days 16–17. We compared the major proteins in the normal equine embryonic capsule and endometrial secretions around the period of fixation with those from pregnancies in the process of termination induced by administration of an analogue of prostaglandin F_2α (PGF_2α). Uterocalin and β₂-microglobulin (β₂M) associated with the embryonic capsule were proteolytically converted to smaller forms during the fixation period. These conversions were similar in conceptuses from control and treated mares. A 17 kDa cationic protein identified as a secretory phospholipase A2 (sPLA2) type IIA was detected bound to normal capsules but increased substantially in response to PGF_2α. Two forms of uteroglobin were distinguished by partial amino acid sequences of ∼6 kDa bands in flush fluids from normal pregnant uteri. After administration of PGF_2α one immunoreactive form of uteroglobin was preferentially increased. These studies demonstrate that failure of pregnancy in this model is associated with an increase in secretory phospholipase in the capsule and a change in the forms of uteroglobin in the uterine secretions.  相似文献   

Prevalence of the AMHR2 mutation in Miniature Schnauzers and genetic investigation of a Belgian Malinois with persistent Müllerian duct syndrome          下载免费PDF全文

MM Smit  KJ Ekenstedt  KM Minor  CK Lim  PAJ Leegwater  E Furrow 《Reproduction in domestic animals》2018,53(2):371-376

Persistent Müllerian duct syndrome (PMDS) is a sex‐limited disorder in which males develop portions of the female reproductive tract. Important consequences of PMDS are cryptorchidism and its sequelae of infertility and increased risk of testicular cancer. Anti‐Müllerian hormone (AMH) and its receptor (AMHR2) induce the regression of the Müllerian ducts in male embryos. In Miniature Schnauzer dogs, the genetic basis has been identified as an autosomal recessive nonsense mutation in AMHR2, but the allele frequency of the mutation is unknown. Thus, the primary objective of this study was to estimate the prevalence of the AMHR2 mutation in North American Miniature Schnauzers, in order to ascertain the value of genetic testing in this breed. An additional objective was to determine whether mutations in AMH or AMHR2 were responsible for PMDS in a Belgian Malinois; this would aid development of a genetic test for the Belgian Malinois breed. Genomic DNA from 216 Miniature Schnauzers (including one known PMDS case) was genotyped for the AMHR2 mutation, and DNA from a single PMDS‐affected Belgian Malinois was sequenced for all coding exons of AMH and AMHR2. The Miniature Schnauzer cohort had an AMHR2 mutation allele frequency of 0.16 and a carrier genotypic frequency of 0.27. The genetic basis for PMDS in the Belgian Malinois was not determined, as no coding or splicing mutations were identified in either AMH or AMHR2. These findings support a benefit to AMHR2 mutation testing Miniature Schnauzers used for breeding or with cryptorchidism.  相似文献   

Automated identification of intensive animal production locations from aerial photography          下载免费PDF全文

KJ Sheffield  JC Hunnam  TN Cuzner  EM Morse‐McNabb  SM Sloan  J Nunan  J Smith  W Harvey  H Lewis 《Australian veterinary journal》2018,96(9):323-331

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Outbreaks of diarrhoea (‘winter scours’) in weaned Merino sheep in south‐eastern Australia          下载免费PDF全文

KJ Stanger  H McGregor  JWA Larsen 《Australian veterinary journal》2018,96(5):176-183

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Effects of Surface Oxidation and Oxygen on the Removal of Trichloroethylene From the Gas Phase Using Elemental Iron     

Uludag-Demirer  Sibel  Bowers  Alan R. 《Water, air, and soil pollution》2003,142(1-4):229-242

The reduction of trichloroethylene (TCE) in the gas phase byFe° was examined under water vapor saturatedconditions (relative humidity (RH) = 100%). The reactionconformed to first-order rate kinetics under anaerobic(N₂ atmosphere) conditions and acid-washedFe°exhibited a faster TCE removal rate than unamended (partially oxidized) Fe°, i.e., k_obs = 0.015 h^-1 versus0.012 h^-1. Analysis of the two types of Fe° showedthat 40.3% of the unamended Fe° surface was nonreactive. Experiments with iron oxides, which form commonly on the surface of Fe° exposed to humid air (magnetiteand maghemite), showed that these solids were nonreactive with TCE under anaerobic conditions. Under aerobic conditions (air),TCE reduction occurred in two distinct phases. There was a fastinitial rate followed by a slower later rate of reduction when the oxide layer was formed. Further experiments showed that theFe° surface was saturated with TCE at higher concentrations (K_1/2 = 5,397.4 ± 345.4 ppmv) and thatoxygen acted as an irreversible inhibitor of TCE reduction(maximum rate of reaction decreased when oxygen was present).  相似文献   

Single-molecule DNA sequencing of a viral genome   总被引：4，自引：0，他引：4  

Harris TD  Buzby PR  Babcock H  Beer E  Bowers J  Braslavsky I  Causey M  Colonell J  Dimeo J  Efcavitch JW  Giladi E  Gill J  Healy J  Jarosz M  Lapen D  Moulton K  Quake SR  Steinmann K  Thayer E  Tyurina A  Ward R  Weiss H  Xie Z 《Science (New York, N.Y.)》2008,320(5872):106-109

The full promise of human genomics will be realized only when the genomes of thousands of individuals can be sequenced for comparative analysis. A reference sequence enables the use of short read length. We report an amplification-free method for determining the nucleotide sequence of more than 280,000 individual DNA molecules simultaneously. A DNA polymerase adds labeled nucleotides to surface-immobilized primer-template duplexes in stepwise fashion, and the asynchronous growth of individual DNA molecules was monitored by fluorescence imaging. Read lengths of >25 bases and equivalent phred software program quality scores approaching 30 were achieved. We used this method to sequence the M13 virus to an average depth of >150x and with 100% coverage; thus, we resequenced the M13 genome with high-sensitivity mutation detection. This demonstrates a strategy for high-throughput low-cost resequencing.  相似文献   

Antiallatotropins: inhibition of corpus allatum development     

Bowers WS  Martinez-Pardo R 《Science (New York, N.Y.)》1977,197(4311):1369-1371

Treatment of newly eclosed adult milkweed bug (Oncopeltus fasciatus) females with precocene 2 prevents secretion of juvenile hormone by inhibition of postimaginal development of the corpus allatum. Ovarian development which is dependent upon juvenile hormone is prevented or reversed, depending upon the timing of precocene treatment. Juvenile hormone secretion is shown to be related to the development of the corpus allatum.  相似文献   

Evidence from ion chromatography experiments that met-cars are hollow cage clusters     

Lee S  Gotts NG  von Helden G  Bowers MT 《Science (New York, N.Y.)》1995,267(5200):999-1001

Ion chromatography studies were performed to assess various models proposed for the structure of M(8)C(12) species, the met-cars. A laser desorption source was used to make a sequence of titanium-carbon clusters centered around Ti(8)C(12)(+). The Ti(8)C(12)(+) was determined to be a hollow cage cluster, with the dodechadron structure originally propposed termined to be a hollow cage cluster, with the dodecahedron structure originally proposed giving the best fit to experiment; cubic structures could be excluded. Collisional breakup of Ti(8)C(12)(+) yielded only Ti(7)C(12)(+) under the experimental conditions described herein, and modeling indicated that the cage structure was retained. Both Ti(8)C(11)(+) and Ti(8)C(13)(+) were made by the cluster source, and again, dodecahedral-type cage structures were consistent with experiment. The extra carbon atom in Ti(8)C(13)(+) was attached exohedrally to a single titanium atom. No evidence for an endohedral species was found.  相似文献   

Distribution of thiobacillus ferrooxidans and leptospirillum ferrooxidans: implications for generation of acid mine drainage   总被引：1，自引：0，他引：1  

MO Schrenk  KJ Edwards  RM Goodman  RJ Hamers  JF Banfield 《Science (New York, N.Y.)》1998,279(5356):1519-1522

Although Thiobacillus ferrooxidans and Leptospirillum ferrooxidans are widely considered to be the microorganisms that control the rate of generation of acid mine drainage, little is known about their natural distribution and abundance. Fluorescence in situ hybridization studies showed that at Iron Mountain, California, T. ferrooxidans occurs in peripheral slime-based communities (at pH over 1.3 and temperature under 30 degreesC) but not in important subsurface acid-forming environments (pH 0.3 to 0.7, temperature 30 degrees to 50 degreesC). Leptospirillum ferrooxidans is abundant in slimes and as a planktonic organism in environments with lower pH. Thiobacillus ferrooxidans affects the precipitation of ferric iron solids but plays a limited role in acid generation, and neither species controls direct catalysis at low pH at this site.  相似文献