Phytophthora bilorbang sp. nov., a new species associated with the decline of Rubus anglocandicans (European blackberry) in Western Australia     

Sonia Aghighi  Giles E. St. J. Hardy  John K. Scott  Treena I. Burgess 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(4):841-855

A new homothallic Phytophthora species, isolated from rhizosphere soil and roots of declining or dead Rubus anglocandicans (European blackberry) in south-west Western Australia, is described as Phytophthora bilorbang sp. nov. It produces non-papillate sporangia, smooth-walled oogonia containing thick-walled oospores, and paragynous antheridia. Although morphologically similar to several species within ITS Clade 6 and sub-clade II, namely P. gibbosa, P. gregata and P. megasperma, phylogenetic analyses of the ITS, cox1, HSP90, BT and NADH gene regions demonstrate that P. bilorbang sp. nov. is a distinct species. Additionally, P. bilorbang differs from these species in its growth and colony morphology on several media. Pathogenicity tests indicate that P. bilorbang could be responsible for the decline syndrome of blackberry within the Warren and Donnelly River catchments in the south-west of Western Australia.  相似文献   

First report of Phytophthora nicotianae associated with Eucalyptus die-back in South Africa     

B. Maseko  T. Burgess  T. Coutinho  M. Wingfield 《Plant pathology》2001,50(3):413-413

  相似文献   

Hematopoietic prostaglandin D2 synthase in the chicken Harderian gland     

Scott TR  Messersmith AR  McCrary WJ  Herlong JL  Burgess SC 《Veterinary immunology and immunopathology》2005,108(3-4):295-306

The Harderian gland (HG), a sero-mucous secreting organ in the eye orbit, has long been recognized as immunologically important in chickens. During experimentation to characterize immune components of the gland, proteomics analysis revealed the presence of hematopoietic prostaglandin D synthase (H-PGDS). Extraction of total RNA followed by RT-PCR produced cDNA of 597 base pairs. DNA sequencing revealed nucleic acid and predicted amino acid sequences that were 99% aligned with the one published sequence for chicken H-PGDS of the spleen. Alignment with murine, rat, and human H-PGDS were 69, 69, and 66%, respectively. Ocular vaccination of chickens with a Newcastle Disease/Infectious Bronchitis vaccine (Mass.-Ark. Strain) induced an increase in H-PGDS expression determined by real-time PCR. Furthermore, immunohistochemistry of frozen HG sections showed positive stained cells for both H-PGDS and mast cell tryptase in the sub-epithelial cell layers of the HG ducts. Based on the potent vasoactive role of PGD(2), it appears that the chicken HG is a site of active mucosal immunity partially mediated by PGD(2) synthesized by H-PGDS in the gland.  相似文献   

Magnetic resonance imaging characterization of canine splenic lesions     

C. A.  Clifford  E. S. Pretorius  C. Weisse  E. S. Siegelman  K. U. Sorenmo  K. Skorupski  K. Drobatz  K. E. Burgess  R. M. Fred  E. N. Glass  M. Murray  J. R. Solomon 《Veterinary and comparative oncology》2005,3(1):37-38

Introduction:  Splenic lesions are a common finding in veterinary medicine and typically 1/2 to 2/3 of these lesions are malignant. Due to the limited accuracy of ultrasound, unnecessary exploratory surgeries/biopsies may be performed for benign lesions and treatment may be delayed for malignant ones. Splenic lesions are rare in people. MR imaging, with its inherently high soft tissue contrast, is efficacious in imaging the human spleen. We have previously demonstrated the efficacy of MRI to differentiate canine hepatic lesions. In that study 8 splenic lesions were all accurately characterized. This current study represents a further evaluation of splenic lesions.
Methods:  In this prospective study, 27 dogs with splenic lesions were accrued. Histopathological/cytological confirmation of lesions occurred either before or shortly after imaging. MRI clinicians were blinded to histopathology results. MR (General Electric, 1.5 Tesla) images using a variety of sequences were obtained before and after intravenous administration of gadolinium.
Results:  32 lesions (9 malignant, 23 benign) were evaluated in 27 dogs. Lesions were confirmed via histopathology (n = 20) or cytology (n = 12). Benign lesions included, EMH (n = 7), hematoma/hemorrhage (n = 5), lymphoid hyperplasia (n = 9), and hemangioma (n = 2). Malignant lesions included anaplastic sarcoma (n = 3), malignant histiocytosis (n = 2), hemangiosarcoma (n = 2), plasma cell tumor (n = 1) and adenocarcinoma (n = 1). The overall accuracy in differentiating benign from malignant lesions was 88%(29/32 lesions). The overall sensitivity and specificity were 100%(95% CI, 66–100) and 87%(95% CI 66–97).
Conclusions:  Based upon these results, MRI is both sensitive and specific in distinguishing between malignant and benign splenic lesions.  相似文献   

Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells     

Sahle M  Burgess WG  Kidanemariam A 《The Onderstepoort journal of veterinary research》2002,69(3):201-206

This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.  相似文献   

A survey of feline viral upper respiratory tract infections.     

N J MacLachlan  G W Burgess 《New Zealand veterinary journal》1978,26(10):260-261

  相似文献   

Detection of immunological tolerance to bovine spumavirus (BSV) with evidence for salivary excretion and spread of BSV from the tolerant animal   总被引：2，自引：0，他引：2  

I G Kertayadnya  R H Johnson  I Abher  G W Burgess 《Veterinary microbiology》1988,16(1):35-39

A group of 17 Friesian-Holstein steers held in individual pens was examined for evidence of infection with bovine spumavirus (BSV). Serum was examined for specific antibody by 2 serological procedures, and circulatory leucocytes and throat swabs were examined for the presence of circulatory leucocyte-associated BSV (CLAB) and saliva-associated BSV (SAB). Initial tests showed that 7 of the 17 steers had specific antibody to BSV by both serological procedures, and a further steer developed such antibody during the first 3 months of holding the animals in single contiguous pens. All 8 of these specific antibody-positive steers were CLAB positive and SAB negative. Nine steers showed no specific antibody to BSV by either of the 2 serological procedures; 8 of these 9 steers showed no evidence of CLAB or SAB. The exception was one steer which was CLAB- and SAB-positive at each of 30 samplings taken over a period of 9 months observation, whilst remaining specific-antibody free. This steer was classed as immunologically tolerant of BSV, and epidemiological data suggested that lateral spread of infection had originated from this animal through the agency of saliva.  相似文献   

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya     

Thomas Dzeha  Michael John Hall  James Grant Burgess 《Marine drugs》2022,20(2)

Epibiotic bacteria associated with the filamentous marine cyanobacterium Moorea producens were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 (1) (C₄₈H₄₉N₁₃O₉S₆; obs. m/z 1144.21930/572.60381) and micrococcin P2 (2) (C₄₈H₄₇N₁₃O₉S₆; obs. m/z 1142.20446/571.60370) from a strain of Bacillus marisflavi isolated from M. producens’ filaments. Interestingly, most bacteria isolated from M. producens’ filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO₄·5H₂O assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of M. producens differed from L. majuscula strain CCAP 1446/4 and L. majuscula clones. Organic extracts of the epibiotic bacteria Pseudoalteromonas carrageenovora and Ochrobactrum anthropi did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from M. producens identified extracts of B. marisflavi as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (1), discovered in Bacillus cereus ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of M. producens species worldwide and their epibionts. These findings indicate that M. producens-associated bacteria are an overlooked source of antimicrobial compounds.  相似文献   

A protein interaction map of Drosophila melanogaster     

Giot L  Bader JS  Brouwer C  Chaudhuri A  Kuang B  Li Y  Hao YL  Ooi CE  Godwin B  Vitols E  Vijayadamodar G  Pochart P  Machineni H  Welsh M  Kong Y  Zerhusen B  Malcolm R  Varrone Z  Collis A  Minto M  Burgess S  McDaniel L  Stimpson E  Spriggs F  Williams J  Neurath K  Ioime N  Agee M  Voss E  Furtak K  Renzulli R  Aanensen N  Carrolla S  Bickelhaupt E  Lazovatsky Y  DaSilva A  Zhong J  Stanyon CA  Finley RL  White KP  Braverman M  Jarvie T  Gold S  Leach M  Knight J  Shimkets RA  McKenna MP  Chant J  Rothberg JM 《Science (New York, N.Y.)》2003,302(5651):1727-1736

  相似文献   

Prevalence of the causes of fleece derangement among sheep flocks in New South Wales     

PW JOHNSON  JC BORAY  JW PLANT  SC BLUNT† 《Australian veterinary journal》1993,70(6):220-224

SUMMARY An investigation of sheep flocks with unknown histories of fleece derangement, selected at random from the main sheep raising areas of New South Wales, showed that Psorergates ovis was present in 53 of the 177 flocks surveyed and In 162 of the 3495 sheep examined. Of the single causes of fleece derangement, the most prevalent was grass seed contamination (15.7%) followed by Infestation with sheep body lice, Bovicola ovis, (14.7%) and Infestation with Itch mite (7.8%). The prevalence of fleece derangement was significantly higher on sheep In which lice were the only ectoparasites found than on those In which Itch mites were the only ectoparasites present. The greatest prevalence of P ovis was In flocks In the Young, Yass, Mudgee and Tenterfield districts. In most affected flocks, more than one potential cause of fleece derangement was present. Itch mite Infestation was more prevalent in flocks that received no treatment for ectoparasites than in those that had been treated in the year preceding the survey. Our results did not support a commonly held belief among farmers that arsenical compounds control Infestations of Itch mites more effectively than other types of pesticides. There was no significant difference in prevalence of fleece derangement between flocks treated with different pesticide groups and those that received no treatment, but mean fleece derangement score was significantly lower in sheep treated with organophosphates than in untreated sheep. Infestations of lice were significantly more prevalent in sheep from flocks treated with synthetic pyrethroids than from those treated with organophosphates or those that received no treatment.  相似文献