排序方式： 共有46条查询结果，搜索用时 31 毫秒
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Yeomans DK Antreasian PG Barriot J Chesley SR Dunham DW Farquhar RW Giorgini JD Helfrich CE Konopliv AS McAdams JV Miller JK Owen WM Scheeres DJ Thomas PC Veverka J Williams BG 《Science (New York, N.Y.)》2000,289(5487):2085-2088
We determined the mass of asteroid 433 Eros, its lower order gravitational harmonics, and rotation state, using ground-based Doppler and range tracking of the Near Earth Asteroid Rendezvous (NEAR)-Shoemaker spacecraft and images of the asteroid's surface landmarks. The mass of Eros is (6.687 +/- 0.003) x 10(18) grams, which, coupled with our volume estimate, implies a bulk density of 2. 67 +/- 0.03 grams per cubic centimeter. The asteroid appears to have a uniform density distribution. The right ascension and declination of the rotation pole are 11.37 +/- 0.05 and 17.22 +/- 0.05 degrees, respectively, and at least over the short term, the rotation state of Eros is stable with no measurable free precession of the spin pole. Escape velocities on the surface vary from 3.1 to 17.2 meters per second. The dynamical environment of Eros suggests that it is covered with regolith and that one might expect material transport toward the deepest potential wells in the saddle and 5.5-kilometer crater regions. 相似文献
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Isotope-edited NMR of cyclosporin A bound to cyclophilin: evidence for a trans 9,10 amide bond 总被引：2，自引：0，他引：2
S W Fesik R T Gampe T F Holzman D A Egan R Edalji J R Luly R Simmer R Helfrich V Kishore D H Rich 《Science (New York, N.Y.)》1990,250(4986):1406-1409
The binding of a 13C-labeled cyclosporin A (CsA) analog to cyclophilin (peptidyl prolyl isomerase) was examined by means of isotope-edited nuclear magnetic resonance (NMR) techniques. A trans 9,10 peptide bond was adopted when CsA was bound to cyclophilin, in contrast to the cis 9,10 peptide bond found in the crystalline and solution conformations of CsA. Furthermore, nuclear Overhauser effects (NOEs) were observed between the zeta 3 and epsilon 3 protons of the methylleucine (MeLeu) residue at position 9 of CsA and tryptophan121 (Trp121) and phenylalanine (Phe) protons of cyclophilin, suggesting that the MeLeu9 residue of CsA interacts with cyclophilin. These results illustrate the power of isotope-edited NMR techniques for rapidly providing useful information about the conformations and active site environment of inhibitors bound to their target enzymes. 相似文献
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Mirjam Helfrich Bernard Ludwig Martin Potthoff Heiner Flessa 《Soil biology & biochemistry》2008,40(7):1823-1835
We investigated the effect of plant residue decomposability and fungal biomass on the dynamics of macroaggregate (250–2000 μm) formation in a three months' incubation experiment and determined the distribution of residue-derived C and N in the microbial biomass and in aggregate size fractions (250–2000 μm, 53–250 μm and <53 μm) using 13C and 15N data. A silty loam soil (sieved <250 μm) was incubated with and without addition of 15N labelled maize leaves (C/N = 27.4) and roots (C/N = 86.4). Each treatment was carried out with and without fungicide application. The addition of maize residues enhanced soil respiration and microbial biomass C and N and resulted in increased macroaggregate formation with a higher and more rapid maximum macroaggregation in the soil amended with maize leaves than in that with addition of roots. Fungicide application led to a significant decline of microbial biomass C and mineralization of the added residues compared to untreated soils, which demonstrates a successful suppression of part of the active microbial biomass by the fungicide. However, this was not confirmed by a generally lower ergosterol concentration. Consequently, ergosterol was no reliable fungal biomarker in periods of rapid decline of the fungal biomass. A single addition of fungicide was insufficient for continued inhibition of the fungal biomass. Yet, a significant delay (28–42 days) in macroaggregation in fungicide treated compared to untreated samples highlighted the importance of the fungal biomass in macroaggregate formation. Macroaggregates were enriched in maize-derived 13C and 15N compared to microaggregates or the fraction < 53 μm. They turned over rapidly with decreasing substrate availability, which entailed a transfer of maize-derived C and N stored within macroaggregates during the first weeks of incubation to microaggregates with proceeding incubation time. Our results indicate that this transfer happened within macroaggregates, because no considerable amount of free particulate organic matter (POM) was released upon macroaggregate breakdown. We conclude that substrate decomposability and fungal activity are key factors determining extent and dynamics of macroaggregation during decomposition processes. Macroaggregate formation implied rapid incorporation and thereby short-term protection of maize-derived C and N. Moreover, macroaggregates allowed a transfer of maize-derived organic matter into microaggregates within macroaggregates, which prevented the release of significant amounts of free POM upon macroaggregate breakdown. Consequently, macroaggregates constitute to the transfer of recently added C into more stable soil organic matter fractions. 相似文献
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Jia-Xuan Yan Qihao Wu Eric J. N. Helfrich Marc G. Chevrette Doug R. Braun Heino Heyman Gene E. Ananiev Scott R. Rajski Cameron R. Currie Jon Clardy Tim S. Bugni 《Marine drugs》2022,20(1)
Chemical investigations of a marine sponge-associated Bacillus revealed six new imidazolium-containing compounds, bacillimidazoles A–F (1–6). Previous reports of related imidazolium-containing natural products are rare. Initially unveiled by timsTOF (trapped ion mobility spectrometry) MS data, extensive HRMS and 1D and 2D NMR analyses enabled the structural elucidation of 1–6. In addition, a plausible biosynthetic pathway to bacillimidazoles is proposed based on isotopic labeling experiments and invokes the highly reactive glycolytic adduct 2,3-butanedione. Combined, the results of structure elucidation efforts, isotopic labeling studies and bioinformatics suggest that 1–6 result from a fascinating intersection of primary and secondary metabolic pathways in Bacillus sp. WMMC1349. Antimicrobial assays revealed that, of 1–6, only compound six displayed discernible antibacterial activity, despite the close structural similarities shared by all six natural products. 相似文献