四翅滨藜在干旱荒漠区的引种表现     

李小燕  孔东升  丁丽萍  赵典普  柴薇薇  李彩霞 《防护林科技》2008,(3):41-43

对引种到河西走廊地区的四翅滨藜生态适应性进行了研究,结果表明,四翅滨藜不仅具有饲料营养价值高、生长速度快、生物量大等多种优良特性,而且是适合在甘肃河西乃至西北荒漠、半荒漠地区栽培的耐干旱、耐寒冷和耐盐碱的一种饲料灌木,将在改善生态环境和发展畜牧业等方面具有很大的发展潜力。  相似文献   

民勤绿洲人工治沙林自然更新能力调查研究     

柴尔武 《甘肃林业科技》2010,35(2):38-42

民勤绿洲治沙造林以梭梭、柠条、沙拐枣为主要树种,经多年定点观测研究,结果表明:梭梭、柠条、沙拐枣在民勤地区均能实现一定量的自然更新,更新量与当年降水量及降水的理想分布呈正相关;梭梭更新一般发生在春季,但春夏无有效降水的情况下,秋季也有少量更新,而柠条、沙拐枣的更新以秋季为主,翌年春季也有更新;3树种自然更新对林木盖度增加几无影响,6年间梭梭、柠条、沙拐枣每hm2株数分别增加了16.7%、8.0%、37.2%;3树种自然更新有明显的地域差异性;个例调查,沙拐枣良好更新具有普遍性,柠条次之,而梭梭只能为个例;更新能力沙拐枣柠条梭梭。  相似文献   

固相微萃取技术在食用植物油分析中的应用     

吴翠蓉  孙楠  柴振林  杨柳  朱杰丽 《浙江林业科技》2013,33(3)

介绍了固相微萃取(SPME)技术的装置device、原理、萃取模式、影响因素、技术特点和应用范围;概述了SPME技术在植物油溶剂残留、香气组分分析、污染物的测定以及评估植物油氧化程度或氧化稳定性中的应用.  相似文献   

干旱胁迫对科罗拉多蓝杉幼树生理特性的影响     

孟凡金  叶景丰  魏忠平  刘怡菲  卜鹏图  郑颖 《北方园艺》2019,(15):76-81

以1年生科罗拉多蓝杉幼树为试材,对其盆栽进行干旱胁迫处理,共设对照(CK,40%水分)、轻度干旱(H1,30%水分)、中轻度干旱(H2,20%水分)、中度干旱(H3,10%水分)和重度干旱(H4,5%水分)5个处理,4周后测定蓝杉幼树的生理特性,以期为合理规划蓝杉苗圃水分补给及选择栽植地提供科学支持。结果表明:1)干旱胁迫干扰了蓝杉幼树叶片有机渗透物质及相对电导率的平衡,脯氨酸含量降低,可溶性糖含量升高;2)干旱胁迫抑制了蓝杉幼树叶片抗氧化酶活性,过氧化物酶及超氧化物歧化酶活性降低,丙二醛含量升高;3)干旱胁迫抑制了蓝杉幼树光合作用速率,叶绿素a、叶绿素b含量及叶片气体交换速率降低;4)在轻度干旱胁迫(H1)下,蓝杉幼树的生长生理特性相较于CK组并没有受到显著影响,但随着胁迫的加剧,其生理及光合作用特性则明显受到干扰;5)在干旱胁迫的作用下,水分不低于20%是蓝衫幼树能够正常生长的阔值。这说明蓝杉可以在轻度干旱地区进行栽植;其育苗及栽植过程中,应避免过于干旱对其造成有害影响。  相似文献   

利用CRISPR/Cas9敲除葡萄VviPDS1基因的研究     

郭晔  万东艳  柴壮壮  王跃进  文颖强 《园艺学报》2019,46(4):623-634

选择葡萄八氢番茄红素脱氢酶(Phytoene desaturase)基因VviPDS1为靶标,利用CRISPR/Cas9系统构建基因敲除载体,瞬时转化葡萄叶片原生质体,检测到不同类型的突变。通过农杆菌介导转化‘无核白’葡萄胚性愈伤组织,筛选获得卡那霉素抗性植株71株。经PCR鉴定,其中53株为阳性植株,阳性率为74.64%。测序结果表明,共有20株在靶点发生不同类型的突变,编辑效率为37.74%;其中9株产生了双等位基因突变。对其进行氨基酸序列预测,在第202位氨基酸之后发生了不同程度的变异。利用CRISPR/Cas9系统敲除VviPDS1获得的突变体植株呈现整体矮化,其叶片出现不同程度白化。表明CRISPR/Cas9系统可以通过细胞中的瞬时或稳定表达进行基因编辑,可以实现在葡萄编辑植株中产生纯合敲除。  相似文献   

Research progress on autophagy in treatment of glioma     

WANG Jing-ping  ZENG Ling-gong  WU Shi-wu  MA Li  CHAI Da-min 《园艺学报》2019,35(5):955-960

Autophagy is a transport pathway from the cytoplasm to the lysosome, which is a major intracellular degradation/recycling system ubiquitous in eukaryotic cells. Autophagy regulation has achieved some gratifying results in the treatment of glioma. It is currently an exciting field of clinical development. In chemotherapy or radiotherapy, autophagy-related drugs are currently used in vitro and in vivo for treating tumors with significant effects. Autophagy inducers and inhibitors may potentially block tumor formation and enhance the anti-cancer immune response. A more comprehensive understanding of the role of autophagy in different stages of glioma development may guide the development of new therapeutic strategies.  相似文献   

瑞丽山龙眼幼苗光合特性研究   总被引：1，自引：0，他引：1  

马加芳  李贵祥  柴勇  和丽萍  孟广涛 《热带作物学报》2017,38(4):640-645

使用Li-6400XT便携式光合仪测定高黎贡山南段2种环境(野外、大棚)下生长的我国特有种植物瑞丽山龙眼幼苗的光合特性,结果表明:(1)2种环境下生长的幼苗净光合速率(PN)日变化出现"双峰"曲线,有"午休"现象,但峰值出现的时刻与大小不同,野外幼苗在10:00、16:00出现高峰值,分别为4.608、2.118μmol/(m~2·s),大棚幼苗在11:00、15:00出现2个高峰值,分别为4.102、3.982μmol/(m~2·s);蒸腾速率(Tr)、气孔导度(Gs)变化趋势与净光合速率变化趋势一致,也有"午休"现象;水分利用率(WUE)无明显变化规律;胞间CO_2浓度(Ci)与净光合速率日变化相反。(2)净光合速率与环境因子偏相关性分析表明,环境因子:光合有效辐射(PAR)、大气CO_2浓度(Ca)、空气温度(Ta)、相对湿度(RH)对幼苗PN值影响均为:PARCaTaRH;(3)两地幼苗光饱和点(LSP)均较高,野外为2 674.995μmol/(m~2·s)、大棚幼苗为2 228.754μmol/(m~2·s),光补偿点(LCP)分别为38.250、8.322μmol/(m~2·s)。  相似文献   

抗除草剂谷子新品种赤谷20的选育及简化栽培技术     

柴晓娇  王显瑞  白晓雷  刘艳春  付颖  沈轶男  李书田 《种子》2017,(8):118-121

利用夏谷区谷子抗除草剂基因,选育适合简化栽培的两系谷子新品种赤谷20.该新品种由抗除草剂拿捕净的新品系k 23-18及不抗除草剂的同型姐妹系k 23-19组成.2014-2015年参加全国谷子品种区域试验,平均单产6 292.50 kg/hm2,较对照谷子品种九谷11(平均单产5 914.5 kg/hm2)增产6.39％.2015年12月通过全国谷子品种鉴定委员会鉴定.通过对辅助除草“剂谷有”用药的研究、对同型姐妹系谷种的最佳混合比例、播种时期、播种量的研究以及对除草剂拿捕净最适宜的喷药时期、喷药浓度、安全剂量等方面研究,制定与其配套的简化栽培技术.确定了同时实现间苗和除草的化学方法,实现全生育期基本不用人工间苗与除草,简化劳动力.  相似文献   

Effects of CENP-W down-regulation on human glioma U87 cells     

JI Qian-kun  LI Jian-bin  FAN Yang-hua  XU Bin  CHAI Yi  JI Chen-xing  ZHU Xin-gen 《园艺学报》2017,33(2):263-270

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.  相似文献   

Neuroprotective effect of fasudil combined with bone marrow-derived neural stem cells on mice with experimental autoimmune encephalomyelitis     

SONG Guo-bin  XI Guo-ping  LI Yan-hua  LI Jia-shan  LIU Jian-chun  CHAI Zhi  XIAO Bao-guo  ZHANG Guang-xian  MA Cun-gen 《园艺学报》2017,33(12):2113-2120

AIM: To explore the neuroprotective effect of fasudil combined with bone marrow-derived neural stem cells (BM-NSCs) on the mice with experimental autoimmune encephalomyelitis (EAE). METHODS: Female C57BL/6 mice (8~10 weeks old, n=32) were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG_35-55) to establish chronic EAE model. The mice were randomly divided into control (ddH₂O) group, fasudil group, BM-NSCs group, and fasudil+BM-NSCs group. The clinical score and body weight were recorded every other day. The expression of neurotrophic factors was determined by immunofluorescence staining. RESULTS: In comparison with ddH₂O group, fasudil combined with BM-NSCs delayed onset and ameliorated severity of EAE. The numbers of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and ciliary neurotrophic factor positive cells in fasudil group, BM-NSCs group and fasudil+BM-NSCs group were all increased in various extents. In particularly, the expression of these neurotrophic factors in fasudil+BM-NSCs group was significantly higher than that in the mice treated with fasudil or BM-NSCs alone (P<0.01). CONCLUSION: Fasudil combined with BM-NSCs promotes the expression of neurotrophic factors and improves microenvironment of central nervous system, thus playing a positive role in neural restoration and regeneration through a synergistic and superimposed effect.  相似文献