Genetic engineering of livestock   总被引：20，自引：0，他引：20  

V G Pursel  C A Pinkert  K F Miller  D J Bolt  R G Campbell  R D Palmiter  R L Brinster  R E Hammer 《Science (New York, N.Y.)》1989,244(4910):1281-1288

Genetic engineering of livestock is expected to have a major effect on the agricultural industry. However, accurate assessment of the consequences of transgene expression is impossible without multigenerational studies. A systematic study of the beneficial and adverse consequences of long-term elevations in the plasma levels of bovine growth hormone (bGH) was conducted on two lines of transgenic pigs. Two successive generations of pigs expressing the bGH gene showed significant improvements in both daily weight gain and feed efficiency and exhibited changes in carcass composition that included a marked reduction in subcutaneous fat. However, long-term elevation of bGH was generally detrimental to health: the pigs had a high incidence of gastric ulcers, arthritis, cardiomegaly, dermatitis, and renal disease. The ability to produce pigs exhibiting only the beneficial, growth-promoting effects of growth hormone by a transgenic approach may require better control of transgene expression, a different genetic background, or a modified husbandry regimen.  相似文献   

Gonadotroph-specific expression of metallothionein fusion genes in pituitaries of transgenic mice   总被引：4，自引：0，他引：4  

M J Low  R M Lechan  R E Hammer  R L Brinster  J F Habener  G Mandel  R H Goodman 《Science (New York, N.Y.)》1986,231(4741):1002-1004

Transgenic mice expressing a metallothionein-somatostatin fusion gene contain high concentrations of somatostatin in the anterior pituitary gland, a tissue that does not normally produce somatostatin. Immunoreactive somatostatin within the anterior pituitaries was found exclusively within gonadotrophs. Similarly, a metallothionein-human growth-hormone fusion gene was also expressed selectively in gonadotrophs. It is proposed that sequences common to the two fusion genes are responsible for the gonadotroph-specific expression.  相似文献   

Overexpression of low density lipoprotein (LDL) receptor eliminates LDL from plasma in transgenic mice   总被引：26，自引：0，他引：26  

S L Hofmann  D W Russell  M S Brown  J L Goldstein  R E Hammer 《Science (New York, N.Y.)》1988,239(4845):1277-1281

A complementary DNA encoding the human low density lipoprotein (LDL) receptor under control of the mouse metallothionein-I promoter was injected into fertilized mouse eggs, and a strain of mice expressing high levels of LDL receptors was established. After administration of cadmium, these mice cleared intravenously injected 125I-labeled LDL from blood eight to ten times more rapidly than did normal mice. The plasma concentrations of apoproteins B-100 and E, the two ligands for the LDL receptor, declined by more than 90 percent after cadmium treatment, but the concentration of another apoprotein, A-I, was unaffected. Therefore, overexpression of an endocytotic receptor can dramatically lower the ambient concentration of its ligand in vivo.  相似文献   

Placers of cosmic dust in the blue ice lakes of greenland     

Maurette M  Hammer C  Reeh N  Brownlee DE  Thomsen HH 《Science (New York, N.Y.)》1986,233(4766):869-872

A concentration process occurring in the melt zone of the Greenland ice cap has produced the richest known deposit of cosmic dust on the surface of the earth. Extraterrestrial particles collected from this region are well preserved and are collectable in large quantities. The collected particles are generally identical to cosmic spheres found on the ocean floor, but a pure glass type was discovered that has not been seen in deep-sea samples. Iron-rich spheres are conspicuously rare in the collected material.  相似文献   

White fat progenitor cells reside in the adipose vasculature   总被引：1，自引：0，他引：1  

Tang W  Zeve D  Suh JM  Bosnakovski D  Kyba M  Hammer RE  Tallquist MD  Graff JM 《Science (New York, N.Y.)》2008,322(5901):583-586

White adipose (fat) tissues regulate metabolism, reproduction, and life span. Adipocytes form throughout life, with the most marked expansion of the lineage occurring during the postnatal period. Adipocytes develop in coordination with the vasculature, but the identity and location of white adipocyte progenitor cells in vivo are unknown. We used genetically marked mice to isolate proliferating and renewing adipogenic progenitors. We found that most adipocytes descend from a pool of these proliferating progenitors that are already committed, either prenatally or early in postnatal life. These progenitors reside in the mural cell compartment of the adipose vasculature, but not in the vasculature of other tissues. Thus, the adipose vasculature appears to function as a progenitor niche and may provide signals for adipocyte development.  相似文献   

Another phocine distemper outbreak in Europe     

Jensen T  van de Bildt M  Dietz HH  Andersen TH  Hammer AS  Kuiken T  Osterhaus A 《Science (New York, N.Y.)》2002,297(5579):209

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Diet-induced hypercholesterolemia in mice: prevention by overexpression of LDL receptors   总被引：22，自引：0，他引：22  

M Yokode  R E Hammer  S Ishibashi  M S Brown  J L Goldstein 《Science (New York, N.Y.)》1990,250(4985):1273-1275

The current studies were designed to determine whether chronic overexpression of low density lipoprotein (LDL) receptors in the liver would protect mice from the increase in plasma LDL-cholesterol that is induced by high-fat diets. A line of transgenic mice was studied that express the human LDL receptor gene in the liver under control of the transferrin promoter. When fed a diet containing cholesterol, saturated fat, and bile acids for 3 weeks, the transgenic mice, in contrast to normal mice, did not develop a detectable increase in plasma LDL. The current data indicate that unregulated overexpression of LDL receptors can protect against diet-induced hypercholesterolemia in mice.  相似文献   

Simple sequence repeats marker polymorphism in emmer wheat (<Emphasis Type="Italic">Triticum dicoccon</Emphasis> Schrank): Analysis of genetic diversity and differentiation     

Yifru Teklu  K. Hammer  M. S. Röder 《Genetic Resources and Crop Evolution》2007,54(3):543-554

Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 different alleles with an average of 12.31 alleles per locus. The number of fragments detected by each primer ranged between 6 (Xgwm1066) and 21 (Xgwm268). Null alleles were detected in nine of the 29 primers used. A high level of gene diversity index was observed. Across the 29 primers, gene diversity ranged from 0.60 (Xgwm46) to 0.94 (Xgwm655), with a mean of 0.82. There was a highly significant correlation (r=0.882; p<0.01) between gene diversity index and the number of loci, showing the number of loci per se is a strong indicator of diversity. Analysis of genetic diversity within and among eleven geographical regions revealed most of the genetic diversity of the total sample resided within regions. The coefficient of gene differentiation (Gst = 0.27) showed that the genetic variation within and among the 11 geographical regions was 73 and 27%, respectively. High value of mean number of alleles per locus was found in Iran (4.86) followed by Morocco (4.10) and Armenia (4.03). On the contrary, lower mean number of alleles per locus was detected in Yemen (2.83). The average gene diversity index across regions ranged from 0.52 (Slovakia) to 0.67 (Morocco) with an average of 0.60. Multivariate techniques of principal component analysis and clustering were employed to examine genetic relationship among the 73 emmer wheat accessions vis-à-vis geographical regions of collections. The genetic distance coefficients for all possible 55 pairs of regional comparisons ranged from 0.63 (between Iran and Armenia, Georgia and Azerbaijan, Georgia and Slovakia) to 0.97 (between Morocco and Yemen, Spain and Georgia, and Turkey and Iran) with a mean of 0.82. From the PCA results, a two dimensional plot of PC1 versus PC2 was constructed. The scatter plot of the first two principal components which explained altogether 27% of the total variation depicted the presence of a clear pattern of geographical differentiation except in few cases like accessions from Caucasian region. Similar pattern of genetic relationships among accessions was observed in cluster analysis. The study provided genetic information of emmer wheat in relation to geographical regions of origin. The information could be utilized in crop improvement, germplasm conservation programs, and in further investigation.  相似文献   

Vorschlag für einen Index der Allogamieneigung bei der Kulturgerste (Hordeum vulgare L. s. l.)     

Karl Hammer 《Genetic Resources and Crop Evolution》1976,24(1):213-220

Zusammenfassung Die Fremdbefruchtungsrate bei Kulturgerste ist in der Regel sehr gering. Einige Anemophiliemerkmale sind aber gut ausgebildet, und unter besonderen Bedingungen kann das Ausmaß der Allogamie recht hoch sein. Aus den blütenbiologischen Merkmalen Antherenejektion (AE), Antherenlänge (AL), und Stigmatafläche (SF) wurden nach zwei verschiedenen Methoden Indices der Allogamieneigung (AN) kalkuliert, mit denen die potentielle Neigung der Gerste zur Allogamie geschätzt werden kann: AN=3,33 (AL·AE)+2,22 (SF·AE) und AN=–3,5+3,58 (AL·AE)+2,29 (SF·AE)Indices der Allogamieneigung werden für ausgewähltes Material des Gaterslebener Gerstensortiments angeführt (Tabellen 1 und 2).

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Proposal for an index of the tendency to allogamy in barley (Hordeum vulgare L. s. l.)

Summary Outcrossing in cultivated barley can be observed relatively seldom. There are some well developed characters of anemophily in barley, however, and under certain conditions a tendency to allogamy can be found. From the flower characters anther extrusion (AE), anther length (AL), and stigma size (SF) in two different manners the following indexes were calculated: AN=3,33 (AL·AE)+2,22 (SF·AE) and AN=–3,5+3,58 (AL·AE)+2,29 (SF·AE)By using these indexes it is possible to demostrate the actual tendency to allogamy in barley. Some results from the barley collection in Gatersleben are shown in tables 1 and 2.

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(Hordeum vulgare L. s. l.)

, , . , , , . , (), (AL) (SF), (AN), : AN=3,33 (AL · AE)+2,22 (SF·AE); AN=–3,5+3,58 (AL·AE)+2,29 (SF·AE); . (. 1 2).

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Genetic Erosion – Examples from Italy<Superscript>1,2</Superscript>     

Karl?Hammer  Gaetano?LaghettiEmail author 《Genetic Resources and Crop Evolution》2005,52(5):629-634

Italy has been used as a country for estimating genetic erosion in crops. It was possible to compare early surveys (from the 1920s to the 1950s), especially on wheats, with results of later missions in the 1980s and 1990s. In the early years, a relatively high genetic erosion was observed (13.2% p.a.). From the 1950s until the 1980s erosion rates between 0.48 and 4% p.a. were estimated. In the little island of Favignana there was an erosion rate of 12.2% p.a. leading to the extinction of the last wheat landraces of this island. There have been no significant differences in erosion rates between field- and garden-crops though there has been the impression that garden crops are better preserved over the long run. Interestingly extinction rates of wild plants (0.13% in the Mediterranean) come close to the average erosion rates of crop plants in the area. ¹Dedicated to Erna Bennett on the occasion of her 80^th birthday ²Lecture presented during the XVII^th Congress of EUCARPIA in Tulln, Austria, 2004  相似文献