Method for Precision Nitrogen Management in Spring Wheat: II. Implementation     

Long  Dan S.  Engel  Richard E.  Carlson  Gregg R. 《Precision Agriculture》2000,2(1):25-38

By accounting for spatial variation in soil N levels, variable-rate fertilizer application may improve crop yield and quality, and N use efficiency within fields. The main purpose of this study was to demonstrate how site-specific wheat yield and protein data, and a geographic information system may be used in developing precision N-recommendations for spring wheat. The three steps in the procedure include: (1) estimate the amount of N-removed in wheat in the year in which the crop is harvested, (2) estimate the N-deficit, defined as the amount of additional N needed for raising protein concentration in a future crop to a specified target level, and (3) estimate the total N-recommendation by summing the mapped values of the N-removed and the N-deficit. A map for variable-rate application of fertilizer is derived by specifying cutoff values to divide the range in the total N-recommendation into classes representing N management zones.A field experiment was conducted within an annually cropped wheat field (101 ha) in northern Montana to determine whether the proposed method could improve grain yields and protein levels. The N-removal and N-deficit were estimated from site-specific wheat yield and protein data that were acquired during harvest of 1996. In 1997, which was a dry year, an experiment was conducted in the same field that consisted of a randomized complete block design arranged as pairs of strip plots. Variable- or uniform-rate N treatments were randomly assigned to each pair of strips. Both treatments received nearly the same amount of fertilizer, however, N in the variable treatment was varied to match patterns in grain yield and protein levels that previously existed in 1996. Yields were not significantly different between management systems, but proteins were significantly enhanced by spatially variable N application. In addition, variability in protein levels was reduced within the whole field. Field areas deficient in N fertility could be identified without having to sample for soil profile N.  相似文献   

Epiphyseal cartilage canal blood supply to the tarsus of foals and relationship to osteochondrosis     

Olstad K  Ytrehus B  Ekman S  Carlson CS  Dolvik NI 《Equine veterinary journal》2008,40(1):30-39

REASON FOR PERFORMING STUDY: Pathological changes in the blood supply to growth cartilage have been implicated in the pathogenesis of osteochondrosis (OC) in horses, but have not been reported using vascular perfusion techniques. OBJECTIVE: To describe the developmental pattern of cartilage canal vessels in the distal tibial epiphysis and talar growth cartilage of foals. METHODS: Nine foals bred from parents with OC were sacrificed between the ages of 0 and 7 weeks to undergo a barium perfusion procedure. The distal end of the tibia and the entire talus were cleared in methyl salicylate and perfused vessels studied in the intact bones. Slabs with a thickness of 4-5 mm from 3 predilection sites for OC were examined in the stereomicroscope and with light microscopy. RESULTS: Cartilage canals were present for a limited period of growth. Perfused vessels initially entered canals from the perichondrium. Vessels in the proximal portion of canals retained their perichondrial arterial source throughout. With time, the ossification front advanced to incorporate the mid-portion of canals; and anastomoses formed between canal vessels and subchondral vessels. A shift occurred and vessels in the distal terminus of canals came to use subchondral vessels as their arterial source. Twelve histological lesions were found in 7 foals. All contained necrotic vessels surrounded by necrotic growth cartilage and 3 caused macroscopically visible delay in endochondral ossification. Lesions were located where vessels traversed the ossification front to enter the distal terminus of canals. CONCLUSION: Cartilage canal vessels are particularly susceptible to failure at the point where they cross the ossification front, with consequences for the viability of those chondrocytes that depend on them. POTENTIAL RELEVANCE: A better understanding of how lesions of OC arise may improve the ability to identify, monitor, prevent and treat this disorder. Involvement of cartilage canals in the pathogenesis of equine tarsal OC plausibly explains several clinical features of this disease.  相似文献   

Aerosol exposure of ammonia, dust and Escherichia coli in broiler chickens.          下载免费PDF全文

O O Oyetunde  R G Thomson    H C Carlson 《The Canadian veterinary journal. La revue veterinaire canadienne》1978,19(7):187-193

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A review of thermal stress in cattle     

RW Shephard  SK Maloney 《Australian veterinary journal》2023,101(11):417-429

Cattle control body temperature in a narrow range over varying climatic conditions. Endogenous body heat is generated by metabolism, digestion and activity. Radiation is the primary external source of heat transfer into the body of cattle. Cattle homeothermy uses behavioural and physiological controls to manage radiation, convection, conduction, and evaporative exchange of heat between the body and the environment, noting that evaporative mechanisms almost exclusively transfer body heat to the environment. Cattle control radiation by shade seeking (hot) and shelter (cold) and by huddling or standing further apart, noting there are intrinsic breed and age differences in radiative transfer potential. The temperature gradient between the skin and the external environment and wind speed (convection) determines heat transfer by these means. Cattle control these mechanisms by managing blood flow to the periphery (physiology), by shelter-seeking and standing/lying activity in the short term (behaviourally) and by modifying their coats and adjusting their metabolic rates in the longer term (acclimatisation). Evaporative heat loss in cattle is primarily from sweating, with some respiratory contribution, and is the primary mechanism for dissipating excess heat when environmental temperatures exceed skin temperature (~36°C). Cattle tend to be better adapted to cooler rather than hotter external conditions, with Bos indicus breeds more adapted to hotter conditions than Bos taurus. Management can minimise the risk of thermal stress by ensuring appropriate breeds of suitably acclimatised cattle, at appropriate stocking densities, fed appropriate diets (and water), and with access to suitable shelter and ventilation are better suited to their expected farm environment.  相似文献   

Development and application of genetically engineered viral vaccines of poultry     

J H Carlson 《Avian diseases》1986,30(1):24-27

Recombinant DNA techniques may completely revolutionize the way vaccines are developed and used. They offer potentially purer, safer, and greater efficacy than many currently used vaccines. This paper describes the various current approaches being used to develop recombinant vaccines. Genes are being cloned into bacteria, yeast, viruses, and eukaryotic cells. Synthetic polypeptides of specific epitopes are also covered. Scientific, practical, economic, and government approval to use recombinant vaccines in the field is also discussed.  相似文献   

Preliminary investigation of alterations in blood viscosity, cellular composition, and electrophoresis plasma protein fraction profile after competitive racing activity in Thoroughbred horses     

C P Coyne  G P Carlson  M S Spensley  J Smith 《American journal of veterinary research》1990,51(12):1956-1963

In this preliminary investigation, various hematologic variables potentially influential in determining the degree of blood viscosity were evaluated in 10 Thoroughbred horses subjected to competitive acute running exercise. Following completion of sprints over a distance of 1.25 miles, mean percent (+/- SD) increases in PCV (38.3 +/- 12.9%), RBC (47.8 +/- 15.3%), and rouleaux index (232.7 +/- 176.8%) were recognized. Simultaneous increases in total plasma protein (28.3 +/- 5.31%), serum albumin (26.7 +/- 6.80%), alpha 1-globulin (60.0 +/- 49.0%), alpha 2-globulin (25.5 +/- 27.9%), beta 1-globulin (46.7 +/- 21.1%), beta 2-globulin (35.0 +/- 50.6%), gamma 1- and 2-globulins (38.7 +/- 29.6%), and plasma fibrinogen (12.5 +/- 10.4%) concentrations increased simultaneously. Horses also had consistent decreases in albumin:globulin ratio (-10.0 +/- 7.43%). Alterations in these hematologic values after acute running exercise in Thoroughbred horses accompanied increases in serum (69.3 +/- 39.7%), plasma (39.7 +/- 11.9%), and blood (134.7 +/- 55.3%) viscosity.  相似文献   

Effect of monensin and supplemental hay on ruminal 3-methylindole formation in adult cows after abrupt change to lush pasture.     

M J Potchoiba  J R Carlson  M R Nocerini  R G Breeze 《American journal of veterinary research》1992,53(1):129-133

The effect of feeding monensin, with or without dry hay plus wilted forage, on ruminal formation of 3-methylindole (3MI) was investigated in pastured cattle. Eighty-two cows were allotted to 3 groups. Cows of group-1 served as controls and were given a daily energy supplement (1 kg/head) without monensin for 1 day before and for 7 days after being allowed access to lush pasture. Cows of groups 2 and 3 were given the same daily energy supplement, which also contained monensin (200 mg/kg of supplement). Cows of group 3 also were fed dry hay for 5 days before the start of the study and continued to be given supplemental hay for 4 days after being allowed access to lush pasture containing a layer of wilted forage. Ruminal 3MI and indole concentrations increased on day 1 after all groups were allowed access to lush pasture. By day 7, 3MI concentration in all cows had decreased to pregrazing concentration. Indole concentration did not reach pregrazing concentration until day 10 for cows of groups 1 and 2. Group-3 cows had pregrazing indole concentration on day 7. Ruminal indole concentration did not differ (P greater than 0.05) between groups 1 and 2. Ruminal indole concentration was lower (P less than 0.01) in group-3 cows on all sample collection days, except day 10, compared with that in the other groups. Monensin reduced (P less than 0.01) 3MI formation on days 1 and 7 in group-2 cows, compared with group-1 cows. Group-3 cows had lower 3MI concentration than did group-1 cows (P less than 0.01) on days -1, 1, 4, and 7.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Clinical and diagnostic features of portosystemic shunt in a foal     

A M Buonanno  G P Carlson  B Kantrowitz 《Journal of the American Veterinary Medical Association》1988,192(3):387-389

Portosystemic shunt was diagnosed in a 6-month-old Quarter Horse filly with acute onset of apparent blindness and a 3-month history of depression, lethargy, and ataxia. Clinicopathologic test results indicated slightly high gamma-glutamyl transpeptidase activity and serum total bilirubin concentration. Sulfobromophthalein half time was prolonged, and plasma ammonia and serum bile acid concentrations were high as well. Histopathologic findings of percutaneous liver biopsy included widespread hepatocyte atrophy and numerous prominent small arterioles in the area of the portal triad. On the basis of history, clinical findings, and clinicopathologic abnormalities, a presumptive diagnosis of portosystemic vascular anomaly was made. To confirm the tentative diagnosis, nuclear hepatic scintigraphy and operative mesenteric portography were performed. Medical treatment was unsuccessful, and the foal was euthanatized. Portosystemic shunts have been described in dogs and cats, but few cases have been reported in large animal species. Other, more common causes of neurologic abnormalities in foals, such as trauma, vertebral body abscesses, brain abscesses, and meningitis, must be ruled out before portosystemic shunt is considered.  相似文献   

Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome.   总被引：6，自引：0，他引：6  

I J Yoon  H S Joo  W T Christianson  H S Kim  J E Collins  J H Carlson  S A Dee 《Journal of veterinary diagnostic investigation》1992,4(2):139-143

Severe clinical signs of swine infertility and respiratory syndrome (SIRS) of unknown cause were observed in several Minnesota swine farms between November 1990 and March 1991. Forty-five lung samples of weak pigs were collected from 13 swine farms, and virus isolation was attempted using swine alveolar macrophage (SAM) cultures. A cytopathic virus was isolated from 19 lung samples collected from 6 different farms. Four pregnant sows were infected intranasally with a tissue suspension from which virus was isolated, and 4 6-week-old pigs and 2 contact pigs were infected intranasally with 1 of the isolates. The 4 sows farrowed 12 stillborn and 32 normal pigs. Virus was recovered from 10 of 19 pigs examined. Infected 6-week-old pigs were clinically normal except for slightly elevated rectal temperatures and mild respiratory signs. No or mild interstitial pneumonic lesions were observed in inoculated pigs, but the lesion was obvious in the 2 contact pigs. Seroconversion was observed in sows and pigs as measured by indirect fluorescent antibody (IFA). Serologic identification of the isolates was carried out by IFA using reference serum prepared from an experimentally infected sow. A cytoplasmic fluorescence was observed on the SAM monolayers infected with each of the 19 different isolates. Fluorescence was also observed when the monolayers were tested with SIRS virus ATCC VR-2332-infected sow sera. Replication of the isolates was not affected in the medium containing 5-iodo-2'-deoxyuridine but was inhibited by treatment with ether. The isolates were relatively stable at 56 C and did not agglutinate with various erythrocytes tested.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Nondestructive Estimation of Standing Crop and Fuel Moisture Content in Tallgrass Prairie     

Sonisa Sharma  Tyson E. Ochsner  Dirac Twidwell  J.D. Carlson  Erik S. Krueger  David M. Engle  Samuel D. Fuhlendorf 《Strength and Conditioning Journal》2018,71(3):356-362

Accurate estimation of standing crop and herbaceous fuel moisture content (FMC) are important for grazing management and wildfire preparedness. Destructive sampling techniques have been used to accurately estimate standing crop and FMC, but those techniques are laborious and time consuming. Existing nondestructive methods for estimating standing crop in tallgrass prairie have limitations, and few studies have examined nondestructive estimation techniques for FMC in this environment. Therefore, our objective was to develop robust models for nondestructive estimation of standing crop and FMC in tallgrass prairie. We calibrated and validated stepwise multiple linear regression (SMLR) and artificial neural network (ANN) models for standing crop and FMC using data collected in tallgrass prairies near Stillwater, Oklahoma. Day of year (DOY), canopy height (CH), Normalized Difference Vegetation Index (NDVI), and percent reflectance in five wavelength bands were candidate input variables for the models. The study spanned two growing seasons and nine patches located within three pastures under patch burn management, and the resulting data set with > 3 000 observations was split randomly with 85% for model calibration and 15% withheld for validation. Standing crop ranged from 0 to 852 g m^? 2, and FMC ranged from 0% to 204%. With DOY, CH, and NDVI as predictors, the SMLR model for standing crop produced a root mean squared error (RMSE) of 119 g m^? 2 on the validation data, while the RMSE of the corresponding ANN model was 116 g m^? 2. With the same predictors, the SMLR model for FMC produced an RMSE of 26.7% compared with 23.8% for the corresponding ANN model. Thus, the ANN models provided better prediction accuracy but at the cost of added computational complexity. Given the large variability in the underlying datasets, the models developed here may prove useful for nondestructive estimation of standing crop and FMC in other similar grassland environments.  相似文献