牦牛脾脏转移因子对小鼠淋巴细胞转化增殖的影响   总被引：1，自引：1，他引：0  

常建军文英  李莉陈德坤  韩海玉 《中国农学通报》2014,30(5):38-40

为了研制一种能够增强动物抗病力的生物活性免疫制剂。采用冻融-透析法提取牦牛脾脏中非特异性转移因子(nTF),并用MTT法检测牦牛TF对小鼠淋巴细胞转化增殖的影响。结果表明：（淋巴细胞+ 刀豆蛋白 +TF）对小鼠脾淋巴细胞的转化增殖效果优于（淋巴细胞+ TF）组和（淋巴细胞+脂多糖+TF）组,差异显著(P<0.05);注射TF组对小鼠脾淋巴细胞的转化增殖的效果优于注射生理盐水对照组 (P<0.05)。牦牛脾脏转移因子对小鼠脾淋巴细胞增殖有促进作用,协同刀豆蛋白(ConA)或脂多糖(LPS)对淋巴细胞增殖的效果有增强趋势。  相似文献   

变量施肥条件下冬小麦长势及品质变异遥感监测   总被引：7，自引：6，他引：1  

宋晓宇  王纪华  黄文江  阎广建  常红 《农业工程学报》2009,25(9):155-162

卫星遥感数据能够在作物生长期内获取大范围“面状”地物光谱信息,反映作物的长势变异情况,以2005－2006年度国家精准农业研究示范基地冬小麦变量施肥试验为基础,以高空间分辨率卫星遥感影像Quickbird为数据源,结合地面获取的冬小麦品质、产量等数据,研究冬小麦长势及品质的变异情况。研究结果表明,Quickbird光谱参数能够反映冬小麦不同施肥处理小区的长势变异,而冬小麦早期的空间长势变异与其最终产量、品质变异有着密切的关系;冬小麦孕穗后期长势光谱信息与其产量有着很好的正相关关系,而与其品质信息存在着显著的负相关关系,其中OSAVI与产量的相关性达到0.536、GNDVI与冬小麦籽粒蛋白质及湿面筋含量的相关性分别达到了-0.531和-0.535;研究还发现,不同植被指数所反映的作物长势存在一定差异,反映冬小麦群体长势的植被参数和反映冬小麦叶绿素密度的植被指数在指示作物空间长势变异上有所不同。因此,利用遥感影像监测作物长势及其品质空间变异在技术上是可行的。  相似文献   

胡敏酸(富啡酸)热力学参数计算     

刘畅  欧阳华 《东北林业大学学报》2004,32(2):89-90

介绍一种计算热力学各参数的方法。它适用于土壤腐植质任何结构,能在标准状况下计算出下列参数：热容Cp,绝对熵S^0、生成熵△S^0、自由能△G^0、生成热△H^0、燃烧热△Q^0。其原理是根据胡敏酸元素组成从热容开始计算,该方法误差小,与实验值之差在允许范围内,有较好的应用价值。提出了芳香度的概念,它是反应胡敏酸和富啡酸分子中线型脂族聚合物片段与芳香性环型聚合物片段的比例,是研究土壤发生、土壤生态、土壤农业极为重要的特征参数。并给出由计算自由能直接计算胡敏酸芳香度的实例。  相似文献   

牛病毒性腹泻病毒(BVDV)抗原捕获ELISA检测方法的建立     

张丽颖  刘畅  鲍永华  赵志辉 《畜牧与兽医》2009,41(8)

用牛病毒性腹泻病毒(BVDV)单克隆抗体包被酶标板,以兔多克隆抗体作为夹心抗体,建立BVDV抗原捕获ELISA(AC-ELISA)检测方法,优化反应条件并对该方法的稳定性等指标进行了测试和评价。结果表明,单抗最佳包被质量浓度为5μg/mL,兔多抗血清最佳质量浓度为10μg/mL;单抗在4℃包被12~24 h,多抗在37℃作用1 h为双抗体的最佳反应条件;酶标抗体最适稀释度1∶10000,最适作用时间为1 h;采用1%BSA和1%明胶分别在抗体包被后和加入待检抗原反应后进行两次封闭效果好。用AC-ELISA方法检测临床采集的11份牛腹泻病料和12份健康牛组织样品,同时以病毒分离和RT-PCR检测方法做对比,3种方法符合率很高。研究表明AC-ELISA方法稳定性好,可用于BVDV的临床快速检测。  相似文献   

Pulmonary Maturation in Canine Foetuses From Early Pregnancy to Parturition     

TM Sipriani  F Grandi  LCG da Silva  PC Maiorka  CI Vannucchi 《Reproduction in domestic animals》2009,44(S2):137-140

Development of the foetal respiratory system includes both pulmonary growth and maturation. In human medicine, a higher incidence of respiratory distress is reported in newborn males. This study aimed to identify different phases of canine foetal lung maturation throughout pregnancy, to determine the stage of pregnancy in which surfactant production begins and to compare pulmonary development of male and female foetuses. Pregnant bitches (34) were subjected to elective ovariohysterectomy and allocated into four groups, according to the stage of pregnancy: 30–40 days of pregnancy (n = 10), 41–50 days (n = 10), 51–60 days (n = 10) and bitches in the first stage of parturition (n = 4). Foetal lungs were histologically processed and evaluated by optical microscopy. The pseudoglandular phase was identified between the 35th day and 46th day of gestation; the onset of canalicular and saccular periods was observed, respectively, from the 48th day and 60th day of pregnancy. Lungs from foetuses at term were in the saccular phase; thus, the development into the alveolar period occurs in the neonatal period. The histological analyses revealed that respiratory tract development is centrifugal, from upper to lower airways. Therefore, it is possible to identify distinct development periods in different portions of the same organ. In conclusion, the saccular phase of lung development begins around 57 and 60 days of pregnancy, the period in which surfactant production is believed to occur. Male and female foetuses present similar pulmonary development from early pregnancy until parturition.  相似文献   

香猪SRY基因的克隆及序列分析     

刘畅 《贵州畜牧兽医》2009,33(4):1-3

试验参照猪SRY基因保守序列设计了1对引物,对香猪基因组DNA进行PCR扩增。将扩增产物通过T—A互补法克隆到质粒pGEM—T载体中,筛选阳性克隆进行DNA测序。测序结果显示,香猪SRY基因开放阅读框全长627bp,编码227个氨基酸。其中核心区域HMG—box长237bp,并且编码79个氨基酸。与白鲸、人、牛、绵羊4种哺乳动物的SRY基因序列相比较,香猪和白鲸的SRY序列具有较高的相似性,相似度达82．1％。序列相似性和邻接法聚类树的结果均显示,在SRY基因中香猪和白鲸有着较近的亲缘关系,表明以白鲸为代表的鲸目和以香猪为代表的偶蹄目有较近的亲缘关系。  相似文献   

大学课堂教学中学生创新能力的培养     

慈恩 《四川畜牧兽医学院学报》2009,(6):158-160

创新人才的培养对于一个国家综合国力的提高有着重要的战略意义。大学是培养创新人才的重要基地,学生创新能力的培养关乎大学教育中人才培养的成败。在我国大学教育中,课堂教学是培养学生创新能力的主要渠道。文中认为营造创新教育环境、引导学生进行探究性学习、培养学生的思维能力以及丰富课堂教学方法和手段等是大学课堂教学中实现学生创新能力培养的重要举措。  相似文献   

不同硼水平对烤烟干物质累积和氮磷钾吸收的影响   总被引：5，自引：0，他引：5  

徐畅  高明  谢德体  宋珍霞  王子芳  代先强 《水土保持学报》2010,24(3)

研究了不同硼浓度下烤烟干物质累积和氮磷钾养分的吸收特点。结果表明,不同硼浓度下烤烟干物质累积和氮磷钾吸收动态均可用Logistic方程描述。硼浓度为0～2.00 mg/L时,各处理烤烟的干物质累积量及氮磷钾吸收量均随着硼浓度的增加而逐渐增加;硼浓度为200～400 mg/L时,各处理烤烟的干物质累积量和氮磷钾吸收量则随着硼浓度的增加而逐渐减少。就烤烟氮磷钾最大累积速率而言,各施硼水平下最大养分累积速率均以钾素最高,氮素其次,磷素的累积速率最低,且硼浓度为0.25～2.00 mg/L的各处理烤烟干物质和氮磷钾养分最大累积速率均高于其他3个处理。就烤烟氮磷钾最大累积速率出现的时间而言,3种元素各不一致,基本表现为硼浓度为0.25～2.00 mg/L各处理烤烟干物质和氮磷钾养分最大累积速率出现时间均晚于其他3个处理。  相似文献   

Accounting for geographic heterogeneity of multifunctional rice policy in Taiwan     

Hung-Hao Chang  Richard N. Boisvert 《Paddy and Water Environment》2006,4(4):229-234

Through empirical simulations of the Taiwanese rice market, we estimate the effects of agricultural policies designed to ensure optimal levels of two multifunctional outputs, groundwater recharge, and methane emissions. Optimal policy instruments differ by region to reflect land quality and the conditions of the aquifers in major rice production regions. The effects on rice production, resource allocation, and returns to farm assets differ as well.

Richard N. BoisvertEmail:

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Isoflavone levels in five soybean (Glycine max) genotypes are altered by phytochrome-mediated light treatments     

Kirakosyan A  Kaufman P  Nelson RL  Kasperbauer MJ  Duke JA  Seymour E  Chang SC  Warber S  Bolling S 《Journal of agricultural and food chemistry》2006,54(1):54-58

The objective of the present study was to determine whether concentrations of different isoflavones (puerarin, genistein, genistin, daidzein, and daidzin) in shoots and roots of five selected soybean genotypes would respond the same or differently to red (650 nm peak transmittance) and far-red (750 nm peak transmittance) light treatments given under controlled environments. Levels of isoflavones (mg g(-1) dry weight biomass) present in seeds, control roots, and shoots and 10 day light-treated seedlings (light, dark, red, and far-red wavelengths) of soybean (Glycine max) were determined by high-performance liquid chromatography analysis in comparison with known isoflavone standards. Seeds of the five soybean genotypes studied consistently stored most of their isoflavones as glucosyl conjugates (e.g., daidzin, genistin, and puerarin). For the five soybean genotypes, isoflavone levels were lower in the seeds as compared with roots plus shoots of control, time zero (first true leaf stage) seedlings. Following 10 days of the respective light treatments, we found that (i) isoflavone levels were enhanced in dark-grown plants over light-grown plants for three of the five genotypes (a new finding) and the reverse occurred for a single genotype (a typical response of legumes) and (ii) generally, far-red end of day (EOD) light treatment enhanced total isoflavone levels in roots plus shoots over red EOD light treatment. Results from the present study show that phytochrome does appear to play a role in regulating isoflavone levels in developing soybean seedlings and that this influence by red/far-red-mediated phytochrome reactions is strongly dependent on the genotypes selected for study.  相似文献