Further characterization of dandelion yellow mosaic virus from lettuce and dandelion     

L. Bos  N. Huijberts  H. Huttinga  D. Z. Maat 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(5):207-222

A damaging virus isolated in the Netherlands from lettuce was studied and compared with a virus isolated from dandelion orginating from Czechoslovakia. It was found to biologically resemble dandelion yellow mosaic virus incompletely described from dandelion and lettuce in Great Britain (Kassanis, 1944, 1947) and from dandelion in Germany (Hein, 1963). Mechanical transmission was greatly improved by buffer solution and transmission byMyzus persicae seemed to be in the non-persistent manner. Longevity in vitro of the virus hardly exceeded one day. Thermal inactivation was between 60 and 65 °C and the dilution end-point was between 10 000 and 100 000. It was still infectious in leaf material dried and stored over CaCl₂ at 4 °C for 6 1/2 years. The virus was isolated and purified with difficulty and was found to consist of one type of spherical particle of ca 30 nm diameter, with a sedimentation coefficient of 159 S, a buoyant density of 1.42 g.cm^?3 and an A₂₆₀/A₂₈₀ ratio of 1.67. An antiserum was prepared with a titre of 256 in the agar double-diffusion test. The virus could be identified in crude extracts from lettuce andChenopodium amaranticolor by enzyme-linked immunosorbent assay (ELISA), but not by agar double diffusion. It could only be visualized in crude sap in the electron microscope after trapping of virus particles on antiserum-coated grids. The virus cannot yet be assigned to any known virus group. It is of potential economic importance to lettuce because of its occurrence in widely differing regions in Europe, its aggressiveness and virulence on 22 out of 23 lettuce cultivars tested (and on endive) and its pathogenicity toLactuca genotypes which are resistant to lettuce mosaic virus and other important pathogens of lettuce. ‘Laibacher Eis’ was the only cultivar showing some tolerance.  相似文献   

Inactivation of foot-and-mouth disease virus in the preparation of a complement fixing antigen     

E E Nikitin  A V Esionov  A I Sobko  V N Prokhorov 《Veterinariia》1968,45(9):20-22

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Arrhenoblastoma in an Indian Desi hen     

B N Gupta  R F Langham 《Avian diseases》1968,12(3):441-444

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Lead poisoning in Canada geese in Delaware   总被引：1，自引：0，他引：1  

G E Bagley  L N Locke  G T Nightingale 《Avian diseases》1967,11(4):601-608

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Studies on the effect of thyroid on vitamin A metabolism. II. Mechanism of decrease of vitamin A in tissues of rats treated with desiccated thyroid     

C Ushimi  N Yoshida 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1964,26(1):7-13

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Inheritance of tolerance to halo blight in bean     

P N Patel  J C Walker 《Phytopathology》1966,56(6):681-682

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Retention of Selenium in Kidneys,Liver and Striated Muscle After Prolonged Feeding of Therapeutic Amounts of Sodium Selenite to Pigs     

P. Lindberg  N. Lannek 《Acta veterinaria Scandinavica》1965,6(1):217

Sodium selenite was mixed in a commercial pig food so as to increase the original selenium content (0.126 ppm) by 1.2 ppm. The supplemented food was given to 7 pigs during 78 days. A control group of as many pigs was given the non-supplemented commercial food. The pigs were killed, starting 4 hours after the last selenium-supplemented food had been given and then with intervals during the next 14 days. Small but significant increases of tissue selenium were noticed in liver and striated muscle as compared with the control animals. The kidneys showed no increase.  相似文献   

An industry in china: technological innovation in china     

Lardy NR 《Science (New York, N.Y.)》1989,244(4902):369

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Magnetic flux transport on the sun     

Wang YM  Nash AG  Sheeley NR 《Science (New York, N.Y.)》1989,245(4919):712-718

Although most of the magnetic flux observed on the sun originates in the low-latitude sunspot belts, this flux is gradually dispersed over a much wider range of latitudes by supergranular convective motions and meridional circulation. Numerical simulations show how these transport processes interact over the 11-year sunspot cycle to produce a strong "topknot" polar field, whose existence near sunspot minimum is suggested by the observed strength of the interplanetary magnetic field and by the observed areal extent of polar coronal holes. The required rates of diffusion and flow are consistent with the decay rates of active regions and with the rotational properties of the large-scale solar magnetic field.  相似文献   

Environmental and behavioural factors affecting the prevalence of foot lameness in New Zealand dairy herds - a case-control study     

Chesterton RN  Pfeiffer DU  Morris RS  Tanner CM 《New Zealand veterinary journal》1989,37(4):135-142

A case-control study of environmental and behavioural factors influencing foot lameness was undertaken on 62 dairy herds comprising an average of 185 milking cows in Taranaki, New Zealand. Thirty two case herds were identified as having had at least 10 per cent of the cows lame during the milking season in which the herd was studied, and thirty control herds were selected on the basis that no more than 3 per cent of cows in these herds had been lame per year for at least two years immediately prior to investigation. Each herd was visited at both a morning and an afternoon milking, and 58 risk factors were measured between the time the farmer began to assemble the cows for milking and the completion of milking. Comparison of single variables between case and control herds identified 24 which showed differences (p<0.10). These variables were then subjected to stepwise multivariate logistic regression, and statistically significant variables in this analysis were used to create a tentative path diagram of possible causal web relationships between the various risk factors and the outcome variable, the lameness prevalence level. Information from a review of the published literature was used to include further variables to the 24 into the initial (or null hypothesis) path model. Logistic path analysis was then used to eliminate non-significant paths from the diagram, leaving 19 arrows joining 13 variables in the final path diagram, compared with 33 joining 20 variables in the initial version. The most influential variables in explaining variation between case and control herds were the average level of maintenance of the track and the degree of patience shown by the farmer in bringing the cows in for milking. Overall, factors associated with the movement of animals to the milking shed explained 40 per cent of the variation (deviance) with regard to the lameness prevalence level. Risk factors associated with characteristics of the milking process explain 24 per cent, and risk factors associated with characteristics of the cows in the herd explain 9.5 per cent. Recommendations are made on management changes which deserve further investigation as ways of reducing lameness problems in dairy herds.  相似文献