Inheritance pattern of chloroplast and mitochondrial genomes in artificial hybrids of Porphyra yezoensis (Rhodophyta)     

Sung-Je Choi  Eun-Jeong Park  Hirotoshi Endo  Yukihiro Kitade  Naotsune Saga 《Fisheries Science》2008,74(4):822-829

ABSTRACT:   To investigate inheritance of chloroplasts and mitochondria (organelles) in the marine red alga Porphyra yezoensis , cleaved amplified polymorphic sequence (CAPS) profiles of the two strains, TU-2 and KGJ, as parental gametophytes and 44 hybrid sporophytes (i.e. female of TU-2 and male of KGJ) were examined. A carbamyl phosphate synthase small subunit gene ( carA ) and ribosomal protein S11 gene ( rps11 )-small subunit ribosomal RNA gene ( rns ) spacer region were used as molecular markers for the chloroplast and mitochondrial DNAs, respectively. Thirty-eight of 44 (86.4%) conchocelis colonies showed maternal banding patterns, five (11.4%) colonies showed biparental bands and one (2.2%) colony showed paternal bands in CAPS analyses both with chloroplast and mitochondrial markers. These results suggest that organelles of P. yezoensis are inherited uniparentally from the female parent.  相似文献   

Acetyl- and butyrylcholinesterase inhibitory activities of sterols and phlorotannins from Ecklonia stolonifera     

Na Y Yoon  Hae Y Chung  Hyeung R Kim  Jae E Choi 《Fisheries Science》2008,74(1):200-207

ABSTRACT:   As part of this study on the isolation of cholinesterase inhibitors from natural marine products, the bioactivity of the ethanolic extracts from 27 Korean seaweeds were screened using acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory assays. Ecklonia stolonifera exhibited promising inhibitory properties against both AChE and BChE. Bioassay-guided fractionation of the active n -hexane and ethyl acetate (EtOAc) soluble fractions, obtained from the ethanolic extract of E. stolonifera , resulted in the isolation of the sterols; fucosterol ( 1 ) and 24-hydroperoxy 24-vinylcholesterol ( 2 ), from the n -hexane fraction and the phlorotannins; phloroglucinol ( 3 ), eckstolonol ( 4 ), eckol ( 5 ), phlorofucofuroeckol-A ( 6 ), dieckol ( 7 ), triphlorethol-A ( 8 ), 2-phloroeckol ( 9 ) and 7-phloroeckol ( 10 ), from the EtOAc fraction. Of these, compounds 2 , 9 and 10 were isolated from E. stolonifera for the first time. Compounds 4 – 7 , 9 and 10 exhibited inhibitory potential against AChE, with 50% inhibition concentration (IC₅₀) values of 42.66 ± 8.48, 20.56 ± 5.61, 4.89 ± 2.28, 17.11 ± 3.24, 38.13 ± 4.95 and 21.11 ± 4.16 μM, respectively; whereas, compounds 1 , 2 , 4 and 6 were found to be active against BChE, with IC₅₀ values of 421.72 ± 1.43, 176.46 ± 2.51, 230.27 ± 3.52 and 136.71 ± 3.33 μM, respectively. It has been suggested that the inhibition of these enzymes by the sterols and phlorotannins derived from marine brown algae could be a useful approach for the treatment of Alzheimer's disease.  相似文献   

The effect of temperature on the energy budget of the Manila clam, <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>     

Kyung Nam Han  Soon Woo Lee  Soon Young Wang 《Aquaculture International》2008,16(2):143-152

In this study, the energy budget of the Manila clam, Ruditapes philippinarum, was evaluated after one-week acclimation periods at 5, 10, 15, 20, and 25°C. Small clams (151 ± 12 mg DW) and large clams (353 ± 16 mg DW) were fed with the microalgae, Isochrysis galbana. Filtration rate, ingestion rate, assimilation efficiency, oxygen-consumption rate, and ammonia excretion rate were measured. Both filtration rate and ingestion rate of small and large clams were found to be related to temperature. The highest Q ₁₀ values were measured in the range 15–20°C for both small and large clams. Assimilation efficiency of both small and large clams was not significantly influenced by temperature, although the maximum mean values were detected at 20°C. Oxygen consumption rate and ammonia excretion rate of small and large clams were found to be related directly to temperature over the entire range, with a maximum being detected at 25°C. The highest Q ₁₀ value was estimated in the range 10–15°C with regard to oxygen consumption rate, and in the range of 15–20°C with regard to ammonia excretion rate. Scope for growth (SFG) was positive at all temperatures, achieving a maximum value at 20°C in both small and large clams, primarily as a consequence of the enhanced ingestion rate which offset the concomitant elevation in the metabolic rate. In this study we have estimated the thermal optimum for this species at 20°C.  相似文献   

The grafting of recycled polyol from waste polyurethane foam onto new polyurethane and its impact on shape recovery and water vapor permeation     

Ha Youn Kim  Jae Won Choi  Yong-Chan Chung  Byoung Chul Chun 《Fibers and Polymers》2017,18(5):842-851

Recycled polyols from waste polyurethane (PU) foams were grafted onto PU to improve the properties such as tensile strength, shape recovery, low-temperature flexibility, and water compatibility. The recycled polyol was either purified by column chromatography before grafting or was used directly for grafting. The soft segment melting temperature of PU did not notably increase with the addition of polyol, whereas the glass transition temperature increased with increased polyol content. The tensile strength sharply increased at low polyol content and decreased at high polyol content, while the strain at break did not significantly change with an increase in polyol content. The shape recovery at 10 oC notably improved compared with unmodified PU and remained high after four cyclic tests. Polyol-grafted PU demonstrated better lowtemperature flexibility and reduced the water vapor permeability of PU membranes. Overall, grafting recycled polyol onto PU significantly improved the tensile stress, shape recovery, and low-temperature flexibility of PU.  相似文献   

Isolation and characterization of nanocrystalline cellulose from different precursor materials     

Byoung-Min Lee  Joon-Pyo Jeun  Phil-Hyun Kang  Jae-Hak Choi  Sung-Kwon Hong 《Fibers and Polymers》2017,18(2):272-277

Nanocrytalline cellulose (NCC) was isolated using cellulose extracted from two different precursor materials: Eucalyptus globulus and rice straw. The two ground precursor materials were autoclaved with a 10 % NaOH solution at 120 °C for 3 h. The alkali-treated precursor materials were bleached using sodium chlorite/acetic acid and sodium hypochlorite. The bleached precursor materials were acid-hydrolyzed in 65 % (w/w) sulfuric acid at 45 °C for 30-120 min. The changes in the chemical composition of the two precursor materials were studied before and after bleaching by Fourier transform infrared spectroscopy according to the NREL report and TAPPI standards. Hydrolyzates were characterized by X-ray diffractometry, thermogravimetric analysis, Zeta-potential analysis, and transmission electron microscopy. The results revealed that the physical properties of NCC were strongly dependent on the acid-hydrolysis time.  相似文献   

Preparation and properties of solid polymer electrolyte based on imidazolium-based ionic liquids for structural capacitors     

Bong-Sang Cho  Jun Choi  Ki-Young Kim 《Fibers and Polymers》2017,18(8):1452-1458

We prepared solid polymer electrolytes (SPEs) composed of poly(ethylene glycol) monomethyl ether acrylate (1A9OMe) and 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIm][OTF]) and 1-ethyl-3-methylimidazolium bis(trifluoromethane sulfonyl) imide ([EMIm][TFSI]) as the ionic liquid. The SPEs formed by appropriately adding ionic liquids in the 1A9OMe prior to thermal cure. The ratio of 1A9OMe and ionic liquid was 1:9, 3:7, and 5:5, respectively. The characterization of solid polymer electrolytes were investigated using Fourier transform infrared spectroscopy in the attenuated total reflectance mode (FTIR-ATR), Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and glavanostatic charge-discharge test. The highest ionic conductivity of SPEs was found to be 4.90×10^?4 S/cm in a 1A9OMe/[EMIm][OTF] of 3:7. As IL contents were increased, the specific capacitance of supercapacitor was increased. The specific capacitance of supercapacitor for ionic liquid with large ion size was lower than that for ionic liquid with smaller ion size.  相似文献   

基于代码生成的电控空气悬架系统电子控制单元   总被引：1，自引：0，他引：1  

严天一  刘欣强  张鲁邹  王玉林  S. B. Choi 《农业机械学报》2014,45(9):14-19

为提高某型SUV车辆的行驶平顺性、通过性等,对其进行空气悬架改装,并设计了由最小系统、车速信号调理模块、电动气泵控制模块、组合电磁阀控制模块、车身高度检测模块、CAN总线模块、车身加速度测量模块等组成的以Freescale XDP512为核心芯片的电控空气悬架系统电子控制单元,利用Real-Time Workshop(RTW)代码生成技术将所制定电控空气悬架系统控制策略转化为ANSI C代码并下载至电子控制单元,然后对安装电控空气悬架系统的试验车辆进行了车身高度与车速耦合试验、转向试验、急加速试验、急减速试验、平顺性试验,结果表明所设计的电控空气悬架系统控制单元能够实现车速信号调理、车身高度与车速耦合、电动气泵控制及组合阀控制等功能。  相似文献   

Isolation and Characterization of a Yellow Xanthophyll Pigment-Producing Marine Bacterium,Erythrobacter sp. SDW2 Strain,in Coastal Seawater     

Sun Wook Jeong  Jung Eun Yang  Yong Jun Choi 《Marine drugs》2022,20(1)

Xanthophylls, a yellow pigment belonging to the carotenoid family, have attracted much attention for industrial applications due to their versatile nature. We report the isolation of a homo xanthophyll pigment-producing marine bacterium, identified as the Erythrobacter sp. SDW2 strain, from coastal seawater. The isolated Erythrobacter sp. SDW2 strain can produce 263 ± 12.9 mg/L (89.7 ± 5.4 mg/g dry cell weight) of yellow xanthophyll pigment from 5 g/L of glucose. Moreover, the xanthophyll pigment produced by the SDW2 strain exhibits remarkable antioxidative activities, confirmed by the DPPH (73.4 ± 1.4%) and ABTS (84.9 ± 0.7%) assays. These results suggest that the yellow xanthophyll pigment-producing Erythrobacter sp. SDW2 strain could be a promising industrial microorganism for producing marine-derived bioactive compounds with potential for foods, cosmetics, and pharmaceuticals.  相似文献   

Cellular Localization of Rice SUMO/SUMO Conjugates and in vitro Sumoylation Using Rice Components     

Joo Joungsu  Hee Choi Dong  Hyon Kim Sang  Ik Song Sang 《水稻科学》2020,27(1):1-4

正Many proteins are regulated by post-translational modifications,such as the reversible covalent attachment of ubiquitin and ubiquitin-like proteins in eukaryotes(Kerscher et al, 2006). Post-translational modification of proteins by the SUMO protein family is involved in diverse cellular processes, including development, hormonal responses, and biotic and abiotic stress signaling(Park et al, 2011). SUMO modification can modulate protein-protein interactions, intracellular localization or the activities of the protein(Gareau and Lima, 2010).  相似文献   

Microbial biomass and activity along a natural pH gradient in forest soils in a karst region of the upper Yangtze River,China     

Ye Tian  Kikuo Haibara  Hiroto Toda  Fangjun Ding  Yanhui Liu  Dongsu Choi 《Journal of Forest Research》2008,13(4):205-214

Mountain closure, considered an effective and economic measure for natural restoration of degraded forest ecosystems, has been widely carried out in the karst region of southwest China. The aim of this study was to evaluate microbial aspects of soil quality after mountain closure by analyzing soil microbial biomass, basal respiration, metabolic quotient, and relationships with basic chemical properties in Guizhou Province, a karst region of the upper Yangtze River. Soil quality was considered poor from the low levels of microbial biomass carbon (MBC), nitrogen (MBN), and microbial quotient (MBC/total C and MBN/total N), but high metabolic quotient (qCO₂). Soil pH, showing marked variation from 4.1 to 7.9 in this karst region, was proved to significantly affect soil microbial biomass and activity. Soil microbial biomass, microbial quotient, and soil basal respiration declined significantly with decreasing soil pH, while qCO₂ showed an apparently increasing, but not statistically significant, trend. The changes in microbial biomass and activity following the change in soil pH could possibly be because of a change in soil microbial composition, and more detailed research is necessary. Compared with soil pH, soil organic matter content was another, more important, factor that directly restricted microbial growth because of the serious loss as a result of disturbance. As a practical application based on microbial aspects, introduction of some N-fixing tree species may be an active and effective measure to improve soil fertility and thus to accelerate restoration of the forest ecosystem in the karst region.  相似文献