Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment     

Dae Young Yoo  Woosuk Kim  Dae Won Kim  Sung Min Nam  Hyo Young Jung  Jong Whi Kim  Choong Hyun Lee  Jung Hoon Choi  Moo-Ho Won  Yeo Sung Yoon  In Koo Hwang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(1):27-33

In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.  相似文献   

Thermal and mechanical properties of modified CaCO3 filled poly (ethylene terephthalate) nanocomposites     

Chang Soo Lee  Kwan Han Yoon  Jae Cheol Park  Hong-Un Kim  Young-Bin Park 《Fibers and Polymers》2014,15(7):1493-1499

Poly(ethylene terephthalate) (PET)/CaCO₃ and PET/modified-CaCO₃ (m-CaCO₃) nanocomposites were prepared by melt blending. The morphology indicated that m-CaCO₃ produced by reacting sodium oxalate and calcium chloride, was well dispersed in PET matrix and showed good interfacial interaction with PET compared to CaCO₃. No significant differences in the thermal properties such as, glass transition, melting and degradation temperatures, of the nanocomposites were observed. The thermal shrinkage of PET at 120 °C was 10.8 %, while those of PET/CaCO₃ and PET/m-CaCO₃ nanocomposites were 2.9–5.2 % and 1.2–2.8 %, respectively depending on filler content. The tensile strength of PET/CaCO₃ nanocomposite decreased with CaCO₃ loading, whereas that of PET/m-CaCO₃ nanocomposites at 0.5 wt% loading showed a 17 % improvement as compared to neat PET. The storage modulus at 120 °C increased from 1660 MPa for PET to 2350 MPa for PET/CaCO₃ nanocomposite at 3 wt% loading, and 3230 MPa for PET/m-CaCO₃ nanocomposite at 1 wt% loading.  相似文献   

Estimating water footprint of paddy rice in Korea   总被引：1，自引：0，他引：1  

Seung-Hwan Yoo  Jin-Yong Choi  Sang-Hyun Lee  Taegon Kim 《Paddy and Water Environment》2014,12(1):43-54

The purpose of this article is to examine the issues of low grain self-sufficiency and the high portion of agricultural water use in South Korea, and to trace the water footprint (WF) of rice products. In this study, different types of water use were described as green, blue, and gray WFs and were analysed using suitable estimation methods to determine irrigation water requirements in South Korea. Virtual water (VW) import and export of rice product were calculated based on international trade statistics during 2004–2009, and the WF of the national consumption was estimated. The WF of rice was 844.5 m³/ton, and green, blue, and gray water accounted for 294.5, 501.6, and 48.4 m³/ton, respectively. The VW import and export were 404.17 and 2.03 Mm³/year, respectively, against an import 199.5 times that of the export. Three countries (China, USA, and Thailand) accounted for over 95 % of the total VW imports of rice products into South Korea. The total WF was 5,712.08 Mm³/year, thus the WF per capita for rice was estimated at 118.1 m³/year. The internal WF of rice consumption was 5,308.05 Mm³/year, and the external WF was 404.03 Mm³/year. The WF of total agricultural water use should be analyzed for sustainable agricultural production and water management, and these results should be applied in establishing long-term policies for agricultural water resources.  相似文献   

Placotylene A,an Inhibitor of the Receptor Activator of Nuclear Factor-κB Ligand-Induced Osteoclast Differentiation,from a Korean Sponge Placospongia sp.     

Hiyoung Kim  Kwang-Jin Kim  Jeong-Tae Yeon  Seong Hwan Kim  Dong Hwan Won  Hyukjae Choi  Sang-Jip Nam  Young-Jin Son  Heonjoong Kang 《Marine drugs》2014,12(4):2054-2065

A new inhibitor, placotylene A (1), of the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation, and a regioisomer of placotylene A, placotylene B (2), were isolated from a Korean marine sponge Placospongia sp. The chemical structures of placotylenes A and B were elucidated on the basis of 1D and 2D NMR, along with MS spectral analysis and revealed as an iodinated polyacetylene class of natural products. Placotylene A (1) displayed inhibitory activity against RANKL-induced osteoclast differentiation at 10 μM while placotylene B (2) did not show any significant activity up to 100 μM, respectively.  相似文献   

Long-term evaluation of extruded diets compared with raw fish moist diet for growing Korean rockfish, Sebastes schlegeli (Hilgendorf)     

Kang-Woong Kim  Xiaojie Wang  So-Min Choi  Sungehul C. Bai  Youn-Hee Choi  & Seong-Hee Choi 《Aquaculture Research》2002,33(12):979-985

A long‐term experiment was conducted in sea cages to compare three experimental extruded pellet (EP) diets with a raw fish moist pellet (MP) diet for Korean rockfish, Sebastes schlegeli (Hilgendorf). White fishmeal (WFM), soybean meal (SM) and fishmeal analogue (BAIFA‐M?)‐based experimental EP diets were formulated to be isonitrogenous and isocaloric with 50% crude protein (CP) with available energy of 16.7 kJ g^?1 diet. An MP diet made of 80% frozen horse mackerel + 20% commercial binder meal was also included in the experiment. The proportions of the major protein ingredients in three experimental EP diets were as follows: WFM diet, 100% WFM; SM diet, 70% WFM + 30% SM; BAIFA‐M? diet, 70% WFM + 30% BAIFA‐M?. The experiment was divided into three periods according to fish size: period I, initial weight of 20.2 ± 3.6 g (mean ± SD); period II, initial weight of 57.6 ± 4.7 g (mean ± SD); period III, initial weight of 96.3 ± 6.9 g (mean ± SD). There were no significant differences in weight gain, feed efficiency ratio, thermal‐unit growth coefficient, specific growth rate, haemoglobin, or haematocrit among fish that were fed the four diets during all three periods. However, fish that were fed the MP diet had a lower survival rate than the fish that were fed the other three EP diets for all periods. These results strongly suggest that EP diets could be developed to replace MP diet for the grow‐out stage of production of Korean rockfish without adverse effects on growth performance.  相似文献   

Attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced gap junctional intercellular communication (GJIC) inhibition in MCF-10A cells by c9,t11-conjugated linoleic acid     

Rakib MA  Kim YS  Jang WJ  Choi BD  Kim JO  Kong IK  Ha YL 《Journal of agricultural and food chemistry》2010,58(22):12022-12030

The protective effect of c9,t11-conjugated linoleic acid (CLA) on the inhibition of gap junctional intercellular communication (GJIC) was examined in a human mammary epithelial cell line (MCF-10A) treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), relative to t10,c12-CLA isomer. TPA inhibited GJIC in a dose-dependent and reversible manner and was associated with connexin 43 phosphorylation. Pretreatment of 20 μM c9,t11-CLA for 24 h prior to 60 nM TPA for 1 h prevented the inhibition of GJIC by reducing the phosphorylation of connexin 43 via suppressing extracellular signal-regulated kinases (ERK1/2) activation. Reactive oxygen species (ROS) accumulation by TPA was attenuated by c9,t11-CLA. The efficacy of c9,t11-CLA in protecting inhibition of GJIC, connexin 43 phosphorylation, and ROS production was superior to that of t10,c12-CLA. These results suggest that c9,t11-CLA, including t10,c12-CLA, prevents the carcinogenesis of MCF-10A cells by protecting down-regulation of GJIC during the cancer promotion stage, and lack of their toxicities could be an excellent indicator for the chemoprevention of breast cancer.  相似文献   

Generation of transgenic homozygous line carrying the CAT gene in mud loach Misgurnus mizolepis     

Yoon Kwon Nam  Young Sun Cho  Young Jin Chang  Jae-Yoon Jo and  Dong Soo Kim 《Fisheries Science》2000,66(1):58-62

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Energy utilisation in germ-free and conventional chicks fed diets containing sorbose     

M Furuse  S I Yang  N Niwa  Y H Choi  J Okumura 《British poultry science》1991,32(2):383-390

1. In experiment 1, growing conventional (CV) chicks were fed on diets containing graded amounts (0, 100, 200 and 300 g/kg diet) of sorbose from 4 to 14 d. Protein, fat and energy deposition were determined after carcase analysis. The values for growth, food efficiency, metabolisable energy (ME) and fat and energy depositions declined as the dietary sorbose content increased. 2. In experiment 2, the performances of germ-free (GF) and CV chicks fed on diets with (100 g sorbose/kg diet) or without sorbose were investigated. On both diets, body weight gain, food consumption and protein accumulation in GF chicks were significantly higher than those in CV birds. No significant differences were observed between the dietary treatment except for ME values, which were significantly lower for the sorbose diet. 3. It is suggested that dietary sorbose decreased energy utilisation, and that the microbial contribution to the utilisation of dietary sorbose was negligible in the chicken.  相似文献   

Detection of classical swine fever virus in boar semen by reverse transcription-polymerase chain reaction.   总被引：6，自引：0，他引：6  

Changsun Choi  Chanhee Chae 《Journal of veterinary diagnostic investigation》2003,15(1):35-41

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Isolation of H3N2 swine influenza virus in South Korea.     

Dae S Song  Jae Y Lee  Jin S Oh  Kwang S Lyoo  Kyung J Yoon  Yong H Park  Bong K Park 《Journal of veterinary diagnostic investigation》2003,15(1):30-34

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