Artificial insemination of Holstein heifers with sex-sorted semen during the hot season in a subtropical region     

Lian-Ben Chang  Chih-Jen Chou  Jia-Shian Shiu  Po-An Tu  Shi-Xuan Gao  Shao-Yu Peng  Shinn-Chih Wu 《Tropical animal health and production》2017,49(6):1157-1162

Our aim was to investigate insemination techniques in order to improve pregnancy rates of artificial insemination (AI) using sex-sorted semen (sexed AI) in cattle in tropical and subtropical (T/ST) regions. In T/ST regions, the pregnancy rates by sexed AI are reportedly the lowest in the hottest months of the year, with less than 15% in cows and 35–40% in heifers (PMID 24048822). We compared sexed AI by depositing the semen into the uterine body (UB-AI, n = 12) versus the unilateral uterine horn (UUH-AI, n = 14) of pre-ovulation heifers. The ovary and follicle were assessed by rectal ultrasound before AI. After insemination, pregnancy was determined by ultrasound at approximately 40 days and approximately 70 days. In the present study, we demonstrated that high pregnancy rates (>70%) by sexed AI in the hottest season in a subtropical region such as Taiwan can be achieved when heifers with pre-ovulation follicles are used. The overall pregnancy rates were 54% higher in the UUH-AI (71%) group than in the UB-AI (42%) group (P = 0.06), examined on approximately 40 days post-sexed AI. Surprisingly, however, the pregnancy outcome appeared to be higher in the hot season (62%) than in the cool season (46%) although this difference was not statistically significant. Based on the present study, we recommend that cattle breeders perform UUH-AI using sex-sorted semen for heifers with pre-ovulation follicles in order to achieve satisfactory pregnancy outcome in the hot seasons in T/ST regions.  相似文献   

Improvement of Fertility in Artificially Inseminated Ewes Following Vaginal Treatment with Misoprostol Plus Terbutaline Sulphate     

AEM Horta  JP Barbas  CC Marques  MC Baptista  MI Vasques  RM Pereira  RD Mascarenhas  S Cavaco‐Gonçalves 《Reproduction in domestic animals》2010,45(6):e412-e416

The effect of vaginal administration of misoprostol plus terbutaline sulphate 6 h prior to artificial insemination (AI) upon the site of AI (vaginal or cervical) and fertility was studied using a total of 87 estrous synchronized Serra da Estrela ewes (control n = 42 and treated n = 45). Artificial insemination was performed using refrigerated semen at 54–55 h after sponge removal. Lambing rate (fertility) and prolificacy were compared between control and treated ewes. The effect of the site of semen deposition on fertility was also evaluated. Prolificacy rate was not different between control (1.5) and treated (1.59) ewes. The proportion of cervical AI achieved in control (45.2%) and treated (37.8%) ewes was not significantly different. Overall, fertility was significantly lower in control than in treated ewes (42.9% vs 64.4%; p < 0.04). Fertility following vaginal AI was significantly lower for control for than treated ewes (30.4% vs 60.7%; p < 0.03) but the difference was smaller and not significant for cervical AI (control 57.9% vs 70.6%). It was concluded that vaginal administration of misoprostol plus terbutaline sulphate 6 h prior to artificial insemination did not affect the proportion of cervical inseminations but significantly improved the fertility of treated ewes. Although needing confirmation, it was hypothesized that drugs might have induced local secretory modifications leading to an increase of cervical ability to retain more viable spermatozoa for fertilization.  相似文献   

Haemoglobin in normal and neoplastic canine mammary glands     

Chang SC  Chen HF  Chou MH  Wang HC  Su HY  Wong ML 《Veterinary and comparative oncology》2010,8(4):302-309

Four types of globins for oxygen transport are known in vertebrates, and the haemoglobin is responsible for carrying oxygen in blood. In this study, we found that haemoglobin was also expressed in canine mammary glands. Samples were taken from 26 malignant mammary tumors, 16 normal mammary glands and 10 other normal tissues. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and mass spectrometry were used to investigate haemoglobin in mammary tissues. The results indicated that normal canine mammary glands expressed high levels of haemoglobin protein as shown by Coomassie blue staining. The identity of haemoglobin was confirmed by immunoblotting and mass spectrometry, and the mass spectrometry data revealed that both alpha-haemoglobin and beta-haemoglobin were expressed. Relative to normal mammary glands, the levels of haemoglobin expression in mammary tumors were lower. Our results also indicated that the haemoglobin was endogenously produced in mammary gland tissues and was not derived from the erythroid cells.  相似文献   

适宜原料压实度改善玉米秸秆厌氧干发酵特性   总被引：1，自引：1，他引：0  

钟慧  丑三涛  朱继英 《农业工程学报》2016,32(22):227-232

干发酵原料中固形物含量高,结构疏松,原料的压实度直接关系到发酵罐的利用率和生产成本。为了探讨原料压实度对厌氧干发酵过程的影响,该文以玉米秸秆粉为试验材料,研究了不同固形物含量(TS)条件下原料压实度对厌氧干发酵产气特性及物料特性变化的影响,结果表明:相同TS下,随着压实度的增加,日产气量(基于可挥发性固形物,VS)峰值降低且出峰时间略有滞后;原料压实度对累积产气量、日产气量变化趋势以及甲烷体积分数的影响不明显。基于发酵罐容积计算产气量时,原料压实度与日产气量峰值、累积产气总量以及累积甲烷产量均呈显著正相关。不同TS条件下的原料压实度对厌氧干发酵过程的影响程度不同,TS含量越高原料压实度对产气量的影响越明显。TS为20%和25%时,逐层压实发酵罐的单位容积累积产气量比自然填料罐分别提高了37.1%和60.2%。当发酵料初始TS为20%时,原料压实度的增加可促使物料中形成连续的液相,改善物料层间的传质,从而使发酵料上下层间的差异减小。当发酵料初始TS提高到25%时,原料压实度增加对传质特性无明显影响。综上所述,在该试验研究范围内,提高原料压实度可以保证玉米秸秆厌氧干发酵产气过程的正常进行,且可以显著提高发酵罐的容积利用率。该研究对厌氧干发酵原料压实度与发酵特性之间的关系进行了初步探讨,以期为有效提高厌氧干发酵罐的产能能力提供参考。  相似文献   

Effect of the Medium Replacement Interval on the Viability,Growth and In Vitro Maturation of Isolated Caprine and Ovine Pre‐Antral Follicles     

DM Magalhães  DD Fernandes  MBS Mororó  CMG Silva  GQ Rodrigues  JB Bruno  MHT Matos  CC Campello  JR Figueiredo 《Reproduction in domestic animals》2011,46(1):134-140

The aim of the present study was to evaluate the effects of different medium replacement intervals on the viability, antral cavity formation, growth and in vitro maturation (IVM) of oocytes from caprine and ovine pre‐antral follicles. Pre‐antral ovarian follicles (≥150 μm) were isolated from the ovarian cortex of goats and sheep and were individually cultured for 24 days using two different medium replacement intervals [2 days (T₁) or 6 days (T₂)]. Follicle development was evaluated on the basis of antral cavity formation, increases in follicular diameter and the presence of healthy cumulus oocyte complexes and fully grown oocytes. For caprine species, results showed a higher percentage (p < 0.05) of viable follicles in T₁ than T₂ from day 6 until the end of the culture. In addition, when comparing both treatments after the same culture duration, the rate of antrum formation was significantly higher in T₁ than in T₂ from day 12 onwards. Yet, in ovines, when both treatments were compared on day 24 of the culture, there were more viable follicles in T₂ than in T₁ (p < 0.05). In the caprine species, percentages of fully grown oocytes (≥110 μm) acceptable for IVM after 24 days of culture were significantly higher in normal follicles cultured in T₁ (30.0%) than in T₂ (6.7%; p < 0.05). On the other hand, in ovines, at the end of the culture, the percentage of oocytes destined for IVM was higher in T₂ than in T₁ (23.5% vs 2.9%; p < 0.05). In conclusion, under the same conditions, the frequency of medium replacement significantly affected the in vitro development of caprine and ovine pre‐antral follicles. To improve the efficiency of the culture system, the medium must be replaced every 2 and 6 days for goat and sheep pre‐antral follicles, respectively.  相似文献   

农业机械化工程研究生创新能力的研究   总被引：1，自引：0，他引：1  

张喜瑞  翁绍捷  李粤  于涛  仇倩倩 《农业装备与车辆工程》2011,(3):37-38,44

研究生教育作为我国教育结构中的高层次的教育,有责任而且有义务为国家培养高水平、高素质,且具备高层次创新能力的人才,教育体系应加强创新教育,逐步提高研究生的创新能力.在了解了农业机械化工程学科现状与进展后,提出了农业机械化工程学科研究生创新能力培养的建议:进行创新性学习,提高创新意识和创新思维;学校改进教学课程体系;多参...  相似文献   

Bioactive cembranoids from the soft coral Sinularia crassa     

Chao CH  Chou KJ  Huang CY  Wen ZH  Hsu CH  Wu YC  Dai CF  Sheu JH 《Marine drugs》2011,9(10):1955-1968

Eight new cembranoids, crassarines A-H (1-8) were isolated from the Formosan soft coral Sinularia crassa. Compounds 1-3 represent the rare cembranoids with a 1,12-oxa-bridged tetrahydrofuran ring, while 4 and 5 are the firstly discovered 1,11-oxa-bridged tetrahydropyranocembranoids. The absolute configuration of 6 was determined using the Mosher's method. Compounds 6 and 8 were found to significantly inhibit the expression of both pro-inflammatory iNOS and COX-2 proteins at 10 μM, respectively, while compounds 4-8 were found to be non-cytotoxic toward the selected human liver cancer cells.  相似文献   

Efficacy of gamma irradiation for protection against postharvest insect damage and microbial contamination of adlay     

Hsiao-Wei Wen  Hsiao-Ping Chung  Ya-Ting Wang  Po-Chow Hsieh  I-Hsin Lin  Fong-In Chou   《Postharvest Biology and Technology》2008,50(2-3):208-215

This study examines the protective effects of gamma irradiation against postharvest insect damage and microbial contamination and its effect on the nutritive value of adlay (Coix lacryma-jobi L.). Adlay was treated with doses of 0–20 kGy gamma irradiation and subsequently stored at ambient temperature. The number of insects, microbial quality and chemical properties of irradiated and non-irradiated adlay were evaluated immediately after treatment and after 6 months of storage. Before irradiation, total aerobic microbial counts ranged from 5.6 × 10² to 1.4 × 10⁵ CFU g⁻¹ and the mean total number of insects was 3.2 ± 2.1 per 100 g of polished kernel. Two kilograys was the lowest dose that provided 100% insect control. A radiation-resistant bacterium, Deinococcus radiodurans RC1, was found in 2 of 10 adlay samples. Four kilograys was a sufficient dose for Enterobacteriaceae inactivation, and 6 kGy was a sufficient dose for yeast and fungi inactivation. Twenty and 8 kGy sufficed for the inactivation of all mesophilic microbes in samples with and without D. radiodurans, separately. Moreover the moisture, ash, crude fiber, crude fat, crude protein and riboflavin content all remained constant. However, 8 kGy irradiation reduced vitamin B1 concentration by 24.2%, but did not measurably reduce the amount of amino acids, except methionine and cysteine. Fatty acid contents did not alter after 8 kGy irradiation, but changes were observed after the 6 months of storage. These changes caused by irradiation were no greater than those caused by the 6-month storage. Irradiation up to 8 kGy did not markedly increase the acid value, but did increase the peroxide value to 13% of the initial value right after irradiation. However, significant changes in acid value and peroxide value were noted after 6 months of storage both in non-irradiated and post-irradiated storage samples. Additionally, 8 kGy irradiation did not significantly change the adlay appearance. The improvement in the hygiene of this vital food source compensates for the small loss of some nutritional constituents. Hence 8 kGy of gamma irradiation can be used in cold decontamination of adlay to prolong shelf-life, to improve postharvest quality, and to reduce the risk of food-borne disease.  相似文献   

半干旱黄土丘陵区典型抗旱造林整地技术集流效果研究   总被引：4，自引：0，他引：4  

蔡进军  李生宝  蒋齐  张源润  许畴 《水土保持研究》2009,16(5)

以宁南山区生态建设中推广应用的典型抗旱造林整地工程为研究对象,针对工程措施的集流效果进行研究,结果表明:实施"88542"集流造林整地工程,能够有效地改善土壤的水分状况,尤其在0-60 cm土层表现较为明显;工程措施的集流效果随地形的变化有所不同:随坡位自上而下改变,"88542"集流水平沟比集流坡面水分的增加率总体上呈增大趋势;随着坡长增大,工程措施对水分增加率也呈增大趋势;随坡度的增大,水平沟相对集流面的水分增加率也随之增加;随坡向不同,工程措施的集流效果则无明显差异;相对于工程措施的集流效果,坡长和坡度之间呈正相关关系,故在工程设计中,坡度越大,相应集流坡面长度就要缩短,反之坡度越缓,集流坡面长度就要增加.  相似文献   

Cloning, functional expression, and characterization of cystatin in sesame seed     

Shyu DJ  Chou WM  Yiu TJ  Lin CP  Tzen JT 《Journal of agricultural and food chemistry》2004,52(5):1350-1356

A cDNA fragment encoding cystatin, a cysteine protease inhibitor, was obtained from maturing sesame seeds. The clone was constructed in a nonfusion or fusion vector and then overexpressed in Escherichia coli. The recombinant cystatins were found in the soluble fraction of cell extract and were demonstrated to be functionally active in a reverse zymographic assay. The corresponding endogenous 22 kDa cystatin of low abundance in mature seeds was purified to homogeneity via a papain-coupling affinity column and confirmed by western blotting with antibodies against the recombinant cystatin. Both endogenous and recombinant cystatin proteins showed effective inhibitory activities against papain with K(i) values of 7.89 x 10(-8) M and 2.77 x 10(-8) M, respectively. Immunodetection indicated that cystatin was specifically expressed in maturing seeds and rapidly degraded in germination. Accordingly, zymographic and inhibition analyses showed that sesame cystatin could not inhibit the de novo synthesized proteases in germinating seeds. It is suggested that sesame cystatin may play a role in the regulation of endogenous cysteine proteases during seed maturation and germination.  相似文献