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Bacteria use conjugation systems, a subfamily of the type IV secretion systems, to transfer DNA to recipient cells. Despite 50 years of research, the architecture and mechanism of action of the channel mediating DNA transfer across the bacterial cell envelope remains obscure. By use of a sensitive, quantifiable assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Agrobacterium tumefaciens. Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage. 相似文献
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Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth 总被引:6,自引:0,他引:6
The aim of this study was to identifiy the endophytic bacteria recovered from the foliage, tap roots and nodules of red clover
plants (Trifolium pratense L.); and to assess the effects of the nodule bacteria, alone and in combination with Rhizobium spp., on the growth and development of red clover seedlings. Thirty-one bacteria species from 14 different genera were recovered
from within the foliage, roots and nodules of red clover plants cv. AC Charlie. Genera diversity and species number were greatest
in foliage tissues. Pantoea agglomerans (59.6%) was the most frequent species recovered in foliage tissues, Agrobacterium rhizogenes A in the tap root (49.2%) and Rhizobium leguminosarum BV phaseoli and R. loti B in the nodules (27.2% each). Recovery of Rhizobium species was not restricted to the nodules, and species of this genus were systemic throughout the plant. Clover root nodules
were host to 12 bacteria species other than rhizobia, of which 8 were specific to this tissue. Using non-selective media,
R. leguminosarum BV trifolii constituted only 8.8% of all the root nodule bacteria recovered. In root bacterization experiments, species of nodule bacteria
promoted growth of red clover more often when applied in combination with R. leguminosarum BV trifolii than when applied singly. However, Bacillus megaterium, Bordetella avium and Curtobacterium luteum consistently promoted growth either individually or in combination with R. leguminosarum BV trifolii. Nodulation was promoted when R. leguminosarum BV trifolii was coinoculated with Bacillus insolitus, B. brevis or A. rhizogenes A. Single isolate applications of Rhizobium species to roots always led to the depression of clover growth, but mixtures of R. leguminosarum BV trifolii and R. leguminosarum BV phaseoli resulted in growth promotion. The latter is considered further evidence of the beneficial allelopathic side effect of strain
competition for the same ecological niche.
Received: 27 June 1996 相似文献
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Norman Q. Arancon Clive A. Edwards Peter Bierman James D. Metzger Stephen Lee Christie Welch 《Pedobiologia》2003,47(5-6):731-735
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Summary Two Digitaria hybrids were compared with pangolagrass, Digitaria decumbens
Stent., using both the light and electron microscope. The purpose of the study was to develop a diagnostic method of screening breeding lines for resistance or tolerance to pangola stunt virus (PSV). Pangolagrass from Surinam and Guyana, naturally infected with PSV, was found to have occassional bundle sheath cells with very thick cell walls. One of the two hybrids, which appeared to be highly susceptible to PSV, also showed similar thick cell walls. It had spherical particles 70 nm in diameter, resembling PSV, maize rough dwarf virus (MRDV) and rice dwarf virus (RDV). Sieve element cells of the phloem were the primary site of particles and the probable site of virus replication. The second hybrid, an apparently resistant line, was found to be completely free of thick-walled bundle sheath cells, and no viruslike particles were found in the bundle sheath or phloem cells. The presence of thick-walled bundle sheath cells appears to offer an excellent diagnostic test for PSV.Journal Paper No 4036 from the Florida Agricultural Experiment Station, Gainesville, Florida. In cooperation with the Plant Virus Laboratory, Department of Plant Pathology, IFAS, University of Florida. 相似文献
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Objective To determine the frequency of the states of depression, anxiety, stress and burnout using internationally validated methods and to relate these to the demographic characteristics of veterinarians in Australia. Methods A postal survey of registered veterinarians with at least one year's experience and whose address was available; 1947 returned the questionnaire providing data for analysis. Results Overall, veterinarians describe higher levels of depression, anxiety, stress and burnout than the general population. The severity of these states was determined by gender, background, type of practice and years after graduation. Conclusions Modifying the curricula of veterinary schools to include the teaching of personal cognitive and coping skills to undergraduate veterinary students, the provision of the opportunity to enhance these skills throughout their veterinary career and changes in the veterinary workplace could result in improved mental health, increased job engagement and work satisfaction. 相似文献
69.
Expression of a cloned rat brain potassium channel in Xenopus oocytes 总被引:21,自引:0,他引:21
Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium. 相似文献
70.
SUMMARY: A survey by mail was used to determine the views of beef producers in the Boophilus microplus endemic area of Queensland on the control of and vaccination against tick fever. Data from 448 questionnaires were analysed, representing 2.7% of beef producers in the survey area. Producers considered buffalo fly ( Haematobia irritans exigua ) infestation as the most important problem whereas tick fever ranked sixth overall. Private veterinarians were regarded as the most important source of information on vaccines for cattle followed by a weekly rural newspaper. From the survey we estimate that about 33% of producers used the tick fever vaccine produced by the Tick Fever Research Centre of Queensland Department of Primary Industries but there were significant (P < 0.05) variations between regions and herds. Large herds (≥ 400 head) in south-east Queensland were the most likely to be vaccinated against tick fever. Of the producers who did not use the vaccine, over 70% replied that there was no need to vaccinate because of the low risk of the disease in their herds. In 52% of unvaccinated herds the treatment of animals with acaricide was considered the most important means of tick fever control and 61% of these herds comprised Bos indicus x Bos taurus or Bos indicus cattle. 相似文献