The Human Genome Project: lessons from large-scale biology   总被引：1，自引：0，他引：1  

Collins FS  Morgan M  Patrinos A 《Science (New York, N.Y.)》2003,300(5617):286-290

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Peroxisomal defects in neonatal-onset and X-linked adrenoleukodystrophies   总被引：13，自引：0，他引：13  

S Goldfischer  J Collins  I Rapin  B Coltoff-Schiller  C H Chang  M Nigro  V H Black  N B Javitt  H W Moser  P B Lazarow 《Science (New York, N.Y.)》1985,227(4682):67-70

Accumulation of very long chain fatty acids in X-linked and neonatal forms of adrenoleukodystrophy (ALD) appears to be a consequence of deficient peroxisomal oxidation of very long chain fatty acids. Peroxisomes were readily identified in liver biopsies taken from a patient having the X-linked disorder. However, in liver biopsies from a patient having neonatal-onset ALD, hepatocellular peroxisomes were greatly reduced in size and number, and sedimentable catalase was markedly diminished. The presence of increased concentrations of serum pipecolic acid and the bile acid intermediate, trihydroxycoprostanic acid, in the neonatal ALD patient are associated with a generalized diminution of peroxisomal activities that was not observed in the patient with X-linked ALD.  相似文献   

Control of embryonic motoneuron survival in vivo by ciliary neurotrophic factor   总被引：22，自引：0，他引：22  

R W Oppenheim  D Prevette  Q W Yin  F Collins  J MacDonald 《Science (New York, N.Y.)》1991,251(5001):1616-1618

During development of the nervous system, neurons in many regions are overproduced by proliferation, after which the excess cells are eliminated by cell death. The survival of only a proportion of neurons during normal development is thought to be regulated by the limited availability of neurotrophic agents. One such putative trophic agent is ciliary neurotrophic factor (CNTF), a polypeptide that promotes the survival of ciliary, sensory, and sympathetic neurons in vitro. In contrast to the results of in vitro studies, however, the daily treatment of chick embryos in vivo with purified human recombinant CNTF failed to rescue any of these cell populations from cell death, whereas CNTF did promote the in vivo survival of spinal motoneurons. Thus, CNTF may not act as a neurotrophic agent in vivo for those embryonic neurons (especially ciliary neurons) on which it acts in vitro. Rather, CNTF may be required for in vivo survival of motoneurons.  相似文献   

Conductance-controlled point functionalization of single-walled carbon nanotubes     

Goldsmith BR  Coroneus JG  Khalap VR  Kane AA  Weiss GA  Collins PG 《Science (New York, N.Y.)》2007,315(5808):77-81

We used covalent attachments to single-walled carbon nanotubes (SWNTs) to fabricate single-molecule electronic devices. The technique does not rely on submicrometer lithography or precision mechanical manipulation, but instead uses circuit conductance to monitor and control covalent attachment to an electrically connected SWNT. Discrete changes in the circuit conductance revealed chemical processes happening in real time and allowed the SWNT sidewalls to be deterministically broken, reformed, and conjugated to target species. By controlling the chemistry through electronically controlled electrochemical potentials, we were able to achieve single chemical attachments. We routinely functionalized pristine, defect-free SWNTs at one, two, or more sites and demonstrated three-terminal devices in which a single attachment controls the electronic response.  相似文献   

Lack of correlation between the levels of soluble cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and the CT-60 genotypes     

Sharad Purohit  Robert Podolsky  Christin Collins  Weipeng Zheng  Desmond Schatz  Andy Muir  Diane Hopkins  Yi-Hua Huang  Jin-Xiong She 《Journal of Autoimmune Diseases》2005,2(1):8

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Correlation of the antinociceptive action and plasma concentration of fentanyl following intravenous administration in cats     

SA Robertson  WL Davies  PM Taylor  MJ Dixon 《Veterinary anaesthesia and analgesia》2002,29(2):94-94

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Lymphocystis disease in snapper (Pagrus auratus) from Spencer Gulf, South Australia     

PJK DURHAM  MJ ALLANSON  WG HUTCHINSON 《Australian veterinary journal》1996,74(4):312-313

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Antibody recognition to secreted proteins of Mycobacterium avium subsp. paratuberculosis in sera from infected ruminants     

M. Pradenas  M.C. Jara  N. Hernndez  A. Zambrano  M.T. Collins  J. Kruze 《Veterinary microbiology》2009,138(3-4):378-383

Two liquid culture media to obtain secreted proteins of Mycobacterium avium subsp. paratuberculosis at different incubation periods were evaluated. Middlebrook 7H9-OADC (7H9) and Watson-Reid (WR) broths were inoculated with a field strain of M. paratuberculosis and growth curves determined using nonlinear regression analysis. Most culture filtrate (CF) proteins were of low molecular weight and reacted strongly against sera from cultured-positive cases of paratuberculosis. CF proteins obtained in WR yielded a higher number of bands and were detected earlier than those obtained from 7H9. A high degree of variability in CF protein immunoreactivity was seen among infected animals. Sera from cattle with clinical paratuberculosis or heavy fecal shedders of M. paratuberculosis reacted more intensively and to more CF proteins than did sera from other infected cattle. Immunoblots showed differences in antibody binding to CF proteins when sera were absorbed with M. avium but not with others environmental mycobacteria. Immunoblots with sera from infected goats and a sheep showed reactivity with proteins of 32, 33 and 46 kDa both before and after the sera were absorbed with M. phlei. Antibodies found in serum of infected deer reacted with CF proteins in a similar way as did for cattle. These results suggest that a pool of CF proteins of M. paratuberculosis could be good candidates as antigens for serodiagnosis of paratuberculosis.  相似文献   

Effects of dietary Camelina sativa products on digestible nutrient compositions for rainbow trout (Oncorhynchus mykiss)          下载免费PDF全文

J.M. Fraser  S.A. Collins  Z. Chen  S.M. Tibbetts  S.P. Lall  D.M. Anderson 《Aquaculture Nutrition》2017,23(5):973-982

Two trials were conducted to determine the effect of dietary inclusion of camelina (Camelina sativa) products on their digestible nutrient compositions in rainbow trout. In the first experiment, the following camelina products: full‐fat seed, oil, high‐oil residue meal (HORM), pre‐press solvent‐extracted meal (SECM) and toasted SECM were evaluated. In the second trial, the utilization of SECM, SECM soaked in water (WS), SECM treated with a multicarbohydrase, Superzyme?‐OM, SECM treated with Bio‐Phytase and SECM treated with Superzyme?‐OM and Bio‐Phytase (MIX) was determined. The experimental diets consisted of a basal diet and test ingredient with a ratio of 70:30, except for camelina oil where the ratio was 80:20. In Experiment 1, SECM had the highest level of digestible crude protein (352 g kg^?1), whereas the oil was the best source of digestible energy (8,063 kcal kg^?1) and digestible crude fat (908 g kg^?1). In Experiment 2, there were no significant differences in nutrient digestibility of SECM and HORM; however, WS and MIX had significantly more digestible dry matter and digestible crude fat than SECM and HORM. Camelina by‐products have the potential to replace fishery by‐products in rainbow trout feeds.  相似文献