Prevalence of Bartonella infection in domestic cats in Denmark   总被引：1，自引：0，他引：1  

Chomel BB  Boulouis HJ  Petersen H  Kasten RW  Yamamoto K  Chang CC  Gandoin C  Bouillin C  Hew CM 《Veterinary research》2002,33(2):205-213

Whole blood and serum from 93 cats (44 pets and 49 shelter/stray cats) from Denmark were tested for the presence of feline Bartonella species by culture and for the presence of Bartonella antibodies by serology. Bartonella henselae was isolated from 21 (22.6%) cats. Bacteremia prevalence was not statistically different between shelter/stray cats (13/49, 26.5%) and pet cats (8/44, 18.2%), but varied widely by geographical origin of the cats, even after stratification for cat origin or age (p < 0.001). All isolates but one were B. henselae type II. The only cat bacteremic with B. henselae type I was not co-infected with B. henselae type II. None of the cats was harboring either B. clarridgeiae or B. koehlerae. Almost half (42/92, 45.6%) of the cats were seropositive for B. henselae and antibody prevalence was similar in shelter/stray cats (23/49, 46.9%) and pet cats (19/43, 44.2%). This is the first report of isolation of B. henselae from domestic cats in Denmark. This study also indicates that domestic cats, including pet cats, constitute a large Bartonella reservoir in Denmark.  相似文献   

Characterization of 26 isolates of Staphylococcus aureus, predominantly from dairy sheep, using four different techniques of molecular epidemiology.     

Eric Vautor  Corinne Jay  Nicolas Chevalier  Nathalie Visomblin  Guy Vernet  Michel Pépin 《Journal of veterinary diagnostic investigation》2005,17(4):363-368

Little information is available regarding the molecular epidemiology of Staphylococcus aureus-induced mastitis in dairy sheep. In this study, 4 different typing techniques were compared in typing 26 S. aureus isolates, predominantly from cases of subclinical mastitis in dairy ewes. The 4 techniques were pulsed-field gel electrophoresis (PFGE), restriction fragment length polymorphism (RFLP) on 2 genes (coagulase and clumping factor B), randomly amplified polymorphic DNA-polymerase chain reaction (PCR) (RAPD-PCR), and multilocus sequence typing (MLST). On the basis of discriminatory power as the key parameter of typing systems, MLST and PFGE were found to be the most powerful techniques. The MLST and PFGE could contribute to epidemiological surveillance and evaluation of mastitis control programs, by documenting prevalence and dissemination of endemic clones in infected populations. The results of this study show that a single clone of S. aureus is widely distributed in infected ewe mammary glands.  相似文献   

Detection and molecular typing of Streptococcus suis in tonsils from live pigs in France     

Corinne Marois  Laëtitia Le Devendec  Marcelo Gottschalk    Marylne Kobisch 《Canadian journal of veterinary research》2007,71(1):14-22

Streptococcus suis is an important pathogen of swine, causing meningitis, arthritis, polyserositis, septicemia, and sudden death in weaning piglets as well as fattening pigs. Recently, 3 molecular tests have been developed in our laboratory: a multiplex polymerase chain reaction (m-PCR) assay for the detection of S. suis species and serotypes 2 and 1/2, and 2 molecular typing methods, pulsed-field gel electrophoresis and an approach based on PCR amplification of a fragment of rRNA genes, including a part of the 16S and 23S genes and the 16S-23S rDNA intergenic spacer region (ISR), followed by restriction fragment length polymorphism (RFLP) analysis (ISR-RFLP). In the present study, we used these tests to analyze tonsil samples from clinically healthy pigs and to identify individual isolates of S. suis during epidemiologic investigations of 8 related herds with a history of septicemia caused by S. suis serotype 2. Capsular typing showed that 58% of the strains were nontypable. Of the 17 serotypes present, serotype 22 was the most prevalent. In the 7 farms without clinical signs on the day of sampling, we detected S. suis serotype 2 or 1/2, or both, in less than 5% of the pigs by m-PCR or by bacteriologic culture. In the 8th farm, on which 2 pigs had clinical signs of septicemia on the day of sampling, we detected S. suis serotype 2 or 1/2, or both, by m-PCR in the tonsils of 40% of fattening pigs (21 wk old) that lacked symptoms. Molecular typing of the serotype 2 strains showed a common origin of contamination in these herds, given that 1 pattern (C1) was detected in the isolates from 6 of the 8 herds. However, up to 4 patterns were associated with septicemia and sudden death. Several patterns of S. suis serotype 2 can be responsible for disease in the same herd. These molecular tools may be useful for confident studies of the transmission of S. suis, thereby contributing to the control of S. suis infection.  相似文献   

HAEMOLYTIC DISEASE OF NEWBORN CALVES IN A DAIRY HERD IN QUEENSLAND     

G. Langford  B.V.Sc.  S. G. Knott  B.V.Sc.  Corinne K.  Dimmock B.Sc.† P. Derrington  B.V.Sc.†† 《Australian veterinary journal》1971,47(1):1-4

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Effect of tibial rotation on the tibial plateau angle measurement in dogs: An ex vivo study     

Corinne L. Buirkle  Karanvir S. Aulakh  J. Alberto Gines  Nathalie Rademacher  Chin‐Chi Liu  Katherine Barnes 《Veterinary surgery : VS》2019,48(3):408-416

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Management of an invasive and metastatic Sertoli cell tumor with associated myelotoxicosis in a dog     

Sita S. Withers  Corinne M. Lawson  Andrew G. Burton  Robert B. Rebhun  Michele A. Steffey 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(3):299-304

We describe the surgical and post-operative management of a large, invasive, and metastatic functional Sertoli cell tumor in a 9-year-old cryptorchid male Labrador retriever dog. Despite residual disease after surgery, bone marrow recovery occurred without administration of bone marrow stimulants and serum estradiol accurately predicted tumor recurrence.  相似文献   

Factors affecting farm operators’ interest in incorporating riparian buffers and forest farming practices in northeast and southeast Missouri   总被引：2，自引：2，他引：0  

Corinne Valdivia  Christine Poulos 《Agroforestry Systems》2009,75(1):61-71

Interest in the incorporation of riparian buffers and forest farming were modeled following agricultural conservation and agroforestry adoption studies. Attitudes, individual characteristics, economic diversity of landowners’ household portfolio, and physical and ecological conditions were explanatory variables in Logit regression models of interest. Habitus and field, the values and institutions of farm operators, were included in the framework. Knowledge of the practice, perceived problems with the environment and attitude/habitus variables had a positive effect on the probability of being interested in riparian buffers. Knowledge of the practice was the most important factor in explaining interest in the case of forest farming, followed by attitudes about trees and concern for future generations, both habitus variables. Field variables like CRP payments representing familiarity with government programs that support conservation, had no significant effect on interest in riparian buffers. Older farmers were less interested in both practices, consistent with other findings. Existing economic diversification of the household portfolio had no effect on the probability of being interested in either practice.

Corinne ValdiviaEmail:

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Response of soil microbial communities to the herbicide mesotrione: A dose-effect microcosm approach     

Olivier Crouzet  Isabelle Batisson  Pascale Besse-Hoggan  Frédérique Bonnemoy  Corinne Bardot  Franck Poly  Clarisse Mallet 《Soil biology & biochemistry》2010,42(2):193-1249

Mesotrione is a new selective herbicide used for maize crops. The responses of microbial communities of a chernozem soil (Limagne basin, France) to pure or formulated (Callisto^®) mesotrione, applied at three different doses [one fold field rate (1 × FR), 10 × FR and 100 × FR], were studied using a laboratory microcosm approach. The effects were assessed on the prokaryotic cell abundance, the overall microbial activities (substrate-induced respiration (SIR) and dehydrogenase activity (DHA)) and the genetic structure of the bacterial and fungal communities (temporal temperature/denaturing gradient gel electrophoresis (TT/DGGE)). Mesotrione dissipation was similar whatever the formulation applied and the amounts dissipated were positively correlated to application rates. Several biodegradation products including the metabolites 4-methylsulfonyl-2-nitrobenzoic acid (MNBA) and 2-amino-4-methylsulfonylbenzoic acid (AMBA) were detected from day 42 post-treatment, in 10 × FR and 100 × FR treated soils. No response of the soil microbial communities was detected in soil spread with both the 1 × FR applications. Overall soil microbial activity was stimulated from day 6 by 10 × FR of Callisto^® and more strongly by 100 × FR of pure mesotrione and Callisto^®, whereas prokaryote abundance did not increase before day 95 in both the 100 × FR treatments. Genetic structural shifts recorded from day 42 in the bacterial and fungal communities were small and mainly attributable to variations in band intensity. Maximum dissimilarity of the bacterial and fungal genetic structures between control and 100 × FR treated soils did not exceed 12% and 28%, respectively. The general pattern was that more consistent effects occurred with increasing exposure times, especially in both the 100 × FR treated soils. These microbial responses could be due to the stimulation of (i) adapted mesotrione-degrading microorganisms and (ii) the activity of resistant heterotrophic microbial groups promoted by dead biomass from sensitive organisms. In addition, at 100 × FR doses, pure mesotrione seemed to induce stronger microbial responses than Callisto^®, formulation which contains adjuvants with potential side-effects on some microbial populations. This experimental approach indicated that pure mesotrione and Callisto^® affected soil microbial communities, but the effects were only detected at doses far exceeding the recommended field rates.  相似文献   

Quantification of six phytoestrogens at the nanogram per liter level in aqueous environmental samples using 13C3-labeled internal standards     

Erbs M  Hoerger CC  Hartmann N  Bucheli TD 《Journal of agricultural and food chemistry》2007,55(21):8339-8345

In light of the estrogenic potentials and the recent concentration levels found for six phytoestrogens in surface waters, detailed monitoring and assessment of potential input sources are required. An accurate, precise, and sensitive HPLC-MS/MS analytical method incorporating five (13)C 3-labeled internal standards for the quantification of these plant estrogens in various aqueous environmental samples is presented here for the first time. The compounds investigated included biochanin A, daidzein, equol, formononetin, genistein, and coumestrol. The use of [ (13)C 3]biochanin A, [ (13)C 3]daidzein, [ (13)C 3]equol, [ (13)C 3]formononetin, and [ (13)C 3]genistein ensured an accurate quantification of the target analytes unaffected by matrix effects and analyte losses. Absolute method recoveries for all analytes ranged from 63 to 105%, from 63 to 99%, and from 73 to 133%, relative recoveries from 90 to 132%, from 89 to 139%, and from 89 to 115%, method detection levels from 0.5 to 2.7 ng/L, from 0.5 to 2.6 ng/L, and from 0.4 to 11.0 ng/L, and precision from 1 to 19%, from 1 to 16%, and from 1 to 11% in drainage water, river water, and WWTP effluent, respectively. The validated analytical method was applied in investigating the emission of the phytoestrogens via drainage water from a pasture containing 43% red clover ( Trifolium pratense) and in monitoring their occurrence in Swiss surface waters. Isoflavone concentrations ranging from 4 to 157 ng/L and up to 22 ng/L were found in drainage and river water, respectively.  相似文献   

In vitro study of adherence, invasion, and persistence of Streptococcus iniae in fibroblastic-like fish cell line SAF-1     

El Aamri F  Real F  Acosta F  Acosta B  Valdivia J  Ramos-Vivas J  Padilla D 《Journal of aquatic animal health》2012,24(3):165-170

Streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. Gentamicin protection assays were used to investigate the ability of different S. iniae strains to invade and adhere to fibroblastic-like fish cell line SAF-1. All strains tested were detected intracellularly using both techniques, with variable internalization degrees between strains. The experiments carried out at 4°C demonstrated that active cell metabolism is necessary for bacterial internalization. Intracellular bacteria were detected for up to 3 d with a round morphology and were stained with 4',6-diamidino-2-phenylindole (DAPI), indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that S. iniae is capable of adhering, entering, and surviving within fibroblastic cells, which may be important for the persistence and establishment of a carrier state.  相似文献